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Additional file 3: Table S1. Correlations of bacterial taxa with clinical and laboratory data. Document S1. Standardized Questionnaire.

Additional file 1: Figure S1. Alpha diversity in postpartum women with (1) or without (0) GDM.

Additional file 2: Figure S2. Alpha diversity in babies with (1) or without (0) exposition to GDM.

Introdução: O câncer mamário é o mais comum entre as mulheres, sendo a segunda maior causa de morte entre esse público. Sua etiologia é bastante variada, estando ligada a causas genética e a fatores de risco ambientais. O exame de escolha para o rastreamento desse câncer é a mamografia, que objetiva a sua detecção precoce em mulheres assintomáticas. A realização da mamografia é recomendada para a população feminina em geral a partir dos 40 anos de idade e com frequência anual. Metodologia: O presente estudo analisou o perfil clinico e epidemiológico das pacientes que realizaram exames mamográficos numa clínica de referência em Teresina-PI. Resultados: Foram analisados, por meio de prontuários eletrônicos, 252 exames de um total de 2.4000 exames realizados em 2019, tendo como critério de exclusão os exames de pacientes com idade inferior a 35 anos. Conclusão: O conhecimento do perfil clínico e epidemiológico das pacientes que realizam exames mamográficos no Piauí é de grande auxílio para a implantação de medidas que visam diagnosticar e tratar precocemente o câncer de mama, possibilitando maior qualidade de vida às pacientes e menos custos ao Estado., Breast cancer. Epidemiology. Health Profile

Purpose: To evaluate the effect of therapeutic resources on the biomechanical characteristics of normal and grafted skin after a burn. Methods: Forty-four participants, both male and female with an average age of 35.89 (±11.53) years, with second- and third-degree burns of both normal skin and scars (skin sheet graft) were evaluated. Participants were randomly chosen, with a crossover design, and a washout of 7 days after each intervention. Biomechanical parameters of the skin, such as distensibility and viscoelasticity, were assessed by Cutometer® before and after 0, 10, 20, and 30 minutes of intervention. This included therapeutic ultrasound and paraffin, with or without vacuum massage. Results: All groups evaluated in this study demonstrated a profile of increased distensibility of their skin in the period immediately after the application of the resources. However, there was a progressive reduction of the effects in the consecutive times tested, most likely due to the probable "therapeutic window”. Participants with scars, showed a decrease in viscoelasticity in all groups except those treated with paraffin. Conclusion: The findings of this study indicate that the post-burn scars after a skin graft present different biomechanical characteristics compared to normal skin, before and after stimulation with various therapeutic interventions.

A mangueira (Mangifera indica L.) é uma planta originária do sudoeste asiático e disseminada em quase todas as regiões do mundo. No Brasil a cultura da mangueira se destaca devido a sua importância econômica, nutricional e social. O Vale do São Francisco, localizado no Nordeste brasileiro, apresenta grande potencial na mangicultura, respondendo por cerca de 66% das exportações brasileiras da fruta. Entretanto, nessa região tem sido relatada a ocorrência de desordens fisiológicas nos frutos da mangueira, como a “manguita” (estenoespermocarpia) que acomete principalmente a cv. Palmer, e cujas causas ainda não estão totalmente elucidadas. A influência do estado nutricional sobre as desordens fisiológicas tem sido comumente observada, em especial, associadas a deficiência de boro. O boro desempenha diversas funções na planta, por fazer parte de compostos essenciais, este micronutriente tem demonstrado influência na produção e qualidade de frutos da mangueira. Neste contexto, entender a relação entre a ocorrência da estenoespermocarpia e a nutrição boratada, é uma necessidade para a cadeia produtiva da manga. Portanto, objetivou-se elaborar uma breve revisão sobre as principais causas do desenvolvimento dessa desordem fisiológica, e a interferência do boro nesse processo. O manejo da adubação com boro apresenta resultados promissores na redução da estenoespermocarpia em mangueira, sendo uma alternativa viável para a mangicultura, uma vez que afeta positivamente a produção e qualidade de frutos de mangueira, no entanto, são necessários estudos para definição das fontes e doses do nutriente a serem aplicadas sob diferentes condições

Memoria de Trabajo fin de Máster presentada por Camila Rodrigues de Carvalho Carvalho, para obtener el Máster en Ingeniería Biomédica por la Universidad Politécnica de Madrid (UPM), realizada bajo la dirección del Dr. Juan Camilo Moreno.-- 70 páginas., Spinal Cord Injury (SCI) is affecting each time a more significant number of people worldwide. People with SCI have compromised their muscle recruitment and are more predisposed to secondary problems. In order to recover or enhance the lower limbs functions, rehabilitation programs are the most used treatment and, more recently, in combination with robots. The use of robots in SCI rehabilitation brings benefits for both the patient and the therapist since it can provide more control, accuracy, and variety of training modes, providing new outcomes to rehabilitation. Electromyography (EMG) activity is often used to trigger orthosis, prosthesis and exoskeletons in robotic rehabilitation. EMG can be recorded using either surface or intramuscular (invasive) detection systems. The use of intramuscular electrodes allows the detection of electric potentials closer to the muscle fibres and the register of EMG activity from deeper muscles. The high selectivity contrariwise, impose also some limitations as the activity reflection of only a small number of active motor units whose fibres are close to the position of the detection site. The European project EXTEND, where this master thesis is inset has, as of purpose, to develop neural interfaced assistive wearable robots for spinal cord injury. In this context, it was performed the analysis of the viability on the use of intramuscular recordings and the characterisation of signal quality and robustness against mechanical interference. Intramuscular electrodes showed a flaw compared to the superficial due to their maintenance in its initial local position, leading to loss of the muscle signal during the task. However, in compensation, they showed less interference from external means (like the exoskeleton) showing cleaner signals of mechanical noise., La lesión de la médula espinal (LME) afecta cada vez a más personas en todo el mundo. Las personas con LME han comprometido su reclutamiento muscular y están más predispuestas a problemas secundarios. Para recuperar o mejorar las funciones de las extremidades inferiores, los programas de rehabilitación son el tratamiento más utilizado y, más recientemente, en combinación con robots. El uso de robots en la rehabilitación con SCI aporta beneficios tanto para el paciente como para el terapeuta, ya que puede proporcionar más control, precisión y variedad de modos de entrenamiento, lo que proporciona nuevos resultados para la rehabilitación. La actividad de electromiografía (EMG) a menudo se utiliza para desencadenar ortesis, prótesis y exoesqueletos en la rehabilitación robótica. La EMG se puede registrar utilizando sistemas de detección de superficie o intramuscular (invasivos). El uso de electrodos intramusculares permite la detección de potenciales eléctricos más cercanos a las fibras musculares y el registro de la actividad EMG de los músculos más profundos. Por el contrario, la alta selectividad impone también algunas limitaciones como el reflejo de la actividad de solo un pequeño número de unidades motoras activas cuyas fibras están cerca de la posición del sitio de detección. El proyecto europeo EXTEND, donde se inserta esta tesis de maestría, tiene como objetivo desarrollar robots portátiles con interfaz neural para la lesión de la médula espinal. En este contexto, se realizó el análisis de la viabilidad en el uso de grabaciones intramusculares y la caracterización de la calidad de la señal y la robustez frente a la interferencia mecánica. Los electrodos intramusculares mostraron una falla en comparación con la superficie debido a su mantenimiento en su posición local inicial, lo que llevó a la pérdida de la señal muscular durante la tarea. Sin embargo, en compensación, mostraron menos interferencia de medios externos (como el exoesqueleto) mostrando señales más limpias de ruido mecánico.

Objective: The purpose of this study was to investigate the presence of postural imbalances, which can interfere with the mandible position and affect the actions of the stomatognathic system. Materials and Methods: Forty men, aged between 23 to 29 years, were selected and divided into two groups: Group I comprised 20 healthy individuals with no clinical postural change diagnosis; Group II, 20 individuals who had ACL surgical, unilateral with patellar tendon graft, paired individual to individual (age and weight). All of them were submitted to an electromyography in different clinical conditions. In addition, a condylar biomechanical assessment was performed through videogrametry. Results: In electromyography and videogrametry, a normal standard biomechanical was observed for both groups, however, Group GII—Ligamentplasty presented an inhibition of the masticatory muscles and decreased joint kinesthetic. Conclusion: Based on the results obtained, it was concluded that after the reconstruction of the ACL, individuals presented changes in the stomatog-nathic system.

Orientadores: Silmara Marques Allegretti, Cláudia Moura de Melo Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia Resumo: As parasitoses gastrintestinais constituem fator limitante à caprinocultura. Este estudo propôs avaliar o potencial antiparasitário da suplementação de sementes de Cucurbita moschata, cascas de Solanum tuberosum, folhas de Musa spp. e polpa cítrica na dieta caprina. Os experimentos foram realizados nos Estados de Sergipe (10°57¿52" S; 37°03¿42" O) e São Paulo (22°57¿26" S; 47°13¿06" W). Os caprinos utilizados nesta pesquisa eram machos (SRD) com infecção natural, 4-6 meses de vida, peso médio de 19,79 ± 5,53Kg e alimentados com ração Guabi® 1kg/animal. Distribuição dos grupos: em Sergipe 30 caprinos: GV/SE ¿ caprinos vermifugados com Ivermectina® - 1mL/25kg; GS/SE ¿ alimentação com 0,8kg/animal de ração + 0,1kg de farinha de semente de C. moschata (FSA) + 0,1kg de farinha da casca de batata S. tuberosum (FCB), adicionados à ração comercial; GC/SE ¿ Controle; em São Paulo 24 caprinos: GV/SP - caprinos vermifugados com Ripercol® - 2mL/20kg e Neguvon® 1mL/20kg; GM/SP - alimentados com 130g de folhas frescas de Musa spp. e 130g de polpa cítrica peletizada; GC/SP - Controle. Os parâmetros analisados foram, contagem de ovos/oocistos por grama de fezes (OPG/OoPG), avaliação clínica individual (Famacha®), avaliação morfométrica, pesagem quinzenal e 3 coletas sanguíneas (dia 0, 37 e 90). Ao final do experimento, os animais eutanasiados tiveram a carcaça limpa pesada, o trato gastrointestinal conduzido ao laboratório para recuperar dos parasitas e parte da musculatura removida para avaliação da composição centesimal. Todos os coprodutos utilizados foram submetidos à análise fitoquimica e de atividade antioxidante. Os resultados foram analisados pelo SAS (? ? 0,05). Em Sergipe foram encontradas espécies de Eimeria sp. (603,4 ± 87,00 OoPG) e a redução do OPG geral foi de 70,73% no GS e 83,82% no GV ao final do experimento sem diferença estatistica (p= 0,074), em relação aos grupos de nematódeos da superfamília Trichostrongyloidea, Strongyloides papillosus e Trichuris sp. a redução foi de 56,42; 86,22 e 97,60% no grupo GV, e 70,47; 61,82 e 98,22% no grupo GS. Este grupo (GS) teve maior ganho de peso (34,86%), de medidas de comprimento corporal (57,10 ± 7,27cm) e altura anterior (58,10 ± 3,81cm), com correlação positiva de 0,65 para o peso final vivo e r = 0,82 para carcaça quente. Os grupos GV e GS apresentaram composição centesimal semelhante. Nos extratos das cascas de S. tuberosum a atividade antioxidante foi IC50 = 0,116mg/mL e nas sementes de C. moschata identificaram-se vitamina E e ácido linoléico. Em São Paulo foram encontradas Eimeria sp. (807,6 ± 78,96 OoPG) e a eficácia nos grupos GM e GV foi de 69,05% e 45,24% respectivamente, ao final do experimento com diferença estatística (p = 0,041). As avaliações hematológicas indicaram leve anemia macrocítica normocrômica no grupo GV no tempo 0, enquanto o grupo GM apresentou monocitose e leucocitose em 62,50% dos caprinos para o mesmo período. O ganho de peso no GM foi 47,59% superior aos demais grupos com p=0,001. Os helmintos recuperados pertenciam as famílias Strongyloidae, Oxiuridae, Trichostrongylidae, Trichuridae e Anaplocephalidae. Os extratos hidroetanólicos das folhas de Musa sp. e da polpa citrica revelaram a presença de rutina, vitamina E, ácidos graxos, cafeico e clorogênico, tais compostos identificados podem ter contribuído com a redução do OPG, a melhoria do aporte produtivo, qualidade da carne (GS/SE) e indução e continuidade da resposta leucocitária frente às infecções helmínticas (GM/SP). Dessa forma, os coprodutos supracitados constituem uma alternativa na diminuição das infecções helmínticas, agregando benefícios econômicos à caprinocultura Abstract: Gastrointestinal parasites are a limiting factor to goat culture. This study aimed to evaluate the antiparasitic and growth potentials, weight gain and hematologic response of goats feed with the supplement of Cucurbita moschata seeds, Solanum tuberosum barks and Musa spp. leaves and citrus pulp. The trials were carried out at the States of Sergipe (10°57'52" S, 37°03'42" W) and São Paulo (22° 57'26" S, 47°13'06" W). Goats used in this research were males (SRD) with natural infection, 4?6 months old, average weight of 19.79 ± 5.53Kg and feed with 1kg/animal of Guabi¿ animal food. Distribution of the groups in Sergipe 30 goats: GV/SE ¿ goats treated with Ivermectina¿ - 1ml/25kg; GS/SE ¿ goats fed with 0.8Kg/animal of the commercial animal food + 100g of C. moschata seed flour (FSA) + 100g of S. tuberosum seed flour (FCB); GC/SE ¿ Control, the experiment lasted 60 days. In São Paulo: 24 goats: GV/SP ¿ goats treated with Ripercol¿ - 2mL/20Kg and Neguvon¿ 1mL/20Kg; GM/SP ¿ feed with 0.740Kg of the commercial animal food + 130g of Musa sp. leaves and 130g citrus pulp; GC/SP ¿ Control, the experiment lasted 90 days. Parameters analyzed fortnightly were counting of eggs/oocysts per gram of feces (OPG/OoPG), clinical individual evaluation (Famacha¿), morphometric analysis, weighing, and 3 blood collections (at days 0, 37 and 90). At the end of the experiment, the goats were euthanized, the cleaned carcasses were weighed, the gastrointestinal tract were taken to the lab (LDIP/Unit ¿ SE and L3/Unicamp ¿ SP) for recovering the parasites and part of the muscle was removed to evaluation of the centesimal composition. All co-products used were examined regarding phytochemical composition and antioxidant activity. The results were analyzed using an analysis of variance (ANOVA) and Pearson¿s correlation coefficient (? ? 0.05). In Sergipe it was found species of Eimeria sp. (603.4 OoPG) and OPG general reduction of 70.73% in the GS and of 83.82% in GV at the end of the experiment without statistical difference (p = 0.074). Groups of nematodes of Trichostrongyloidea superfamily, Strongyloides papillosus and Trichuris sp. presented reduction of 56.42, 86.22 and 97.60% in GV group, and of 70.47, 61.82 and 98.22% in GS group. GS group had the highest weight gain (34.86%), body length measurements (57.10 ± 7.27cm) and height of the withers (58.10 ± 3.81cm), with mean positive correlation of 0.65 for live final weight and r = 0.82 for warm carcass. GV and GS groups presented similar centesimal composition. In extracts from S. tuberosum bark, the antioxidant activity was of IC50 = 0.116mg/mL and vitamin E and linoleic acid were identified from the C. moschata seeds. In São Paulo it was found Eimeria sp. (807.6 ± 78.96 OoPG) and effectiveness in GM and GV groups was of 69.05% and 45.24%, respectively, at the end of the experiment with statistical difference (p = 0.021). Hematologic evaluations revealed discreet normochromic macrocytic anemia in GV group at time 0. Weight gain in GM group was 47.59% higher than the other groups (p = 0.001). Helminthes recovered belonged to the families Strongyloidae, Oxiuridae, Trichostrongylidae, Trichuridae and Anaplocephalidae. By the phytochemical analysis of hydroethanolic extracts from Musa sp. it was identified rutin, coumarin and E vitamin, and in the citric pulp, caffeic acid, coumarin and hesperidin. These identified compounds may have contribute to OPG reduction, to enhance productiveness (growth), meat quality (GS/SE), and induction and continuity of the leukocytarian response against the helminthic infections (GM/SP). In this way, the aforementioned co-products are an alternative for the decrease of helminthic infections, boosting economic benefits to goat culture Doutorado Relações Antrópicas, Meio Ambiente e Parasitologia Doutora em Biologia Animal

Endophytic fungi represent ubiquitous microbial organisms able to live in the tissues of different plants around the world and represent a prolific source of bioactive metabolites. In the present study, the endophytic fungus Aspergillus calidoustus was isolated from the medicinal plant Acanthospermum australe (Asteraceae), and identified using molecular, physiological and morphological methods. A methylene chloride crude extract of A. calidoustus has been produced and subjected to antifungal bioassay-directed fractionation which resulted in the isolation of the two bioactive compounds: ophiobolin K and 6-epi-ophiobolin K. These pure compounds displayed antifungal activity against fungal plant pathogens, protozoal activity against Trypanosoma cruzi, and cytotoxic activity against human tumoral cell lines. The results show that A. calidoustus was able to produce the antifungal and cytotoxic metabolites ophiobolin K and 6-epi-ophiobolin K, which may help the fungus to colonise and occupy the substratum as well as survive in natural environments.

A Depressão na infância e adolescência, tal como na população adulta, é uma das perturbações mentais mais comuns. Uma vez que o seu aparecimento nestas faixas etárias conduz a graves consequências na idade adulta, é fundamental identificar os sintomas depressivos precocemente. Desta forma, os instrumentos de autorrelato têm um papel fundamental, uma vez que permitem com facilidade, de forma fidedigna e válida, ter acesso a formas de pensar, sentir e agir dos sujeitos. O objetivo do presente trabalho consistiu na tradução, adaptação e estudo da Center for Epidemiological Studies Depression Scale for Children (CES-DC) (Weissman, Orvash & Padian, 1980) para os adolescentes portugueses. A amostra é constituída por 417 adolescentes, com idades compreendidas entre os 12 e os 18 anos a frequentar o 3º ciclo do ensino básio e ensino secundário. Para o estudo da validade convergente e divergente da CES-DC, foram utilizados a Depression Anxiety Stress Scales (DASS 21) (Lovibond & Lovibond, 1995), o Children's Depresssion Inventory (CID) (Marujo, 1994) e a Students' Life Satisfaction Scale (SLSS) (Marques, Pais-Ribeiro & Lopez, 2007) que avaliam, respetivamente, os estados emocionais negativos (depressão, ansiedade e stress), a sintomatologia depressiva e a satisfação global com a vida. Os resultados obtidos mostram que a escala possui uma boa consistência interna, uma estabilidade temporal adequada, assim como uma boa validade. Sugerem, ainda, tratar-se de uma escala tridimensional: fator humor, fator interpessoal e fator felicidade. Estes dados permitem o avanço da Psicologia, no que diz respeito à avaliação e prática clínica com adolescentes. A utilidade do questionário deve ser verificada noutras faixas etárias, principalmente, nas de menos de 12 anos de idade, e também numa amostra clínica. Não obstante as limitações apontadas, os resultados sugerem que a CES-DC é um questionário útil na avaliação de sintomas depressivos nos adolescentes. / Depression in children and adolescents, as well as in adults, is one of the most common mental disorders. Since its appearance in these age groups leads to serious consequences in adulthood, it is critical to identify depressive symptoms in early stages. Therefore, self-report instruments play a key role in this contexto since they allow to easily, reliably and validly have access to ways the subject thinks, feels and acts. The aim of this study was to translate, adapt and study the Center for Epidemiological Studies Depression Scale for Children (CES-DC) (Weissman, Orvash & Padian, 1980) for portuguese adolescents. The sample consists of 417 adolescents, aged between 12 and 18 years old, attending the 3rd cycle of basic education and secondary education schools. To study the convergent and divergent validity of the CES-DC, the Depression Anxiety Stress Scales (DASS 21) (Lovibond & Lovibond, 1995) was used, along with the Children's depression Inventory (CDI) (Sailor, 1994) and the Students' Life Satisfaction Scale (SLSS) (Marques, Pais-Ribeiro & Lopez, 2007) that evaluate, respectively, the negative emotional states (depression, anxiety and stress), depressive symptoms and overall satisfaction with life. The results show that the evaluated scale presents a good internal consistency, an adequate temporal stability as well as good validity. They also suggest that this is a three-dimensional scale: humor factor, interpersonal factor and happiness factor. These data allow for the progress of psychology, regarding the assessment and clinical practice among adolescents. The usefulness of the questionnaire should be verified in other age groups, especially in children with less than 12 years old and in a clinical sample. Despite these limitations, the results suggest that CES-DC is useful in the assessment questionnaire of depressive symptoms in adolescents.

Made available in DSpace on 2019-09-12T16:53:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2019 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) In tropical biomes, abrupt cold events frequently occur in subtropical regions and can be associated with large daily thermal fluctuations. The bromeliad Nidularium minutum Mez is a cold tolerant species from the Atlantic Rainforest, which grows in the subtropical latitude, where temperatures range from 2 to 30 degrees C. We hypothesized that N. minutum plants would implement rapid metabolic adjustments to ensure survival during sudden cold events that occur in seasons other than winter. N. minutum plants were cultivated at 10 degrees C. Under this condition, there was an observed increase in soluble sugar content and nitric oxide (NO) emission, as well as a high nitrate reductase (NR) activity observed within the 72 h of cold exposure, when compared with plants maintained at 25 degrees C. These responses coincided with a decrease in antioxidant activity and an increase in lipid peroxidation (LPO). After 72 h of cold exposure, a subset of plants were de-acclimated at 25 degrees C. These plants displayed reduced soluble sugar concentrations, NO emissions, NR activity and LPO. Additionally, there was an increase in anti-oxidant activity, which indicated that the plants were recovering from cold stress. Furthermore, it was found that plants maintained at 10 degrees C for 168 h acclimated to the cold by reducing the NO content and maintaining the increased sugar concentrations, thus resulting in a less intense stress response. We conclude that rapid changes in NO content, sugar concentrations and antioxidant activity are metabolic adjustments that occur during the acclimation and de-acclimation processes of this bromeliad to low temperatures. During abrupt thermal changes in subtropical regions, such adjustments could enhance cold tolerance and increase the chances of survival for this plant. [Carvalho, Camila Pereira; Nievola, Catarina Carvalho] Inst Bot SMA SP, Nucleo Pesquisa Plantas Ornamentals, Ave Miguel Stefano 3687, BR-04301902 Sao Paulo, SP, Brazil [Cardoso-Gustayson, Poliana] Univ Fed ABC, Ctr Ciencias Nat & Humans, BR-09606070 Sao Bernardo Do Campo, Brazil [Rodrigues, Edson] Universidade de Taubaté (Unitau), Inst Basico Biociencias, BR-12030180 Taubate, SP, Brazil [Braga, Marcia Regina] Inst Botan SMA SP, Nucleo Pesquisa Fisiol & Bioquim, BR-04301902 Sao Paulo, SP, Brazil [Mercier, Helenice] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo, SP, Brazil