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Microplastics are small plastic fragments that are widely distributed in marine and terrestrial environments. While the soil ecosystem represents a large reservoir for plastic, research so far has focused mainly on the impact on aquatic ecosystems and there is a lack of information on the potentially adverse effects of microplastics on soil biota. Earthworms are key organisms of the soil ecosystem and are due to their crucial role in soil quality and fertility a suitable and popular model organism in soil ecotoxicology. Therefore, the aim of this study was to gain insight into the effects of environmentally relevant concentrations of microplastics on the earthworm Eisenia andrei on multiple levels of biological organization after different exposure periods. Earthworms were exposed to two types of microplastics: (1) polystyrene-HBCD and (2) car tire abrasion in natural soil for 2, 7, 14 and 28d. Acute and chronic toxicity and all subcellular investigations were conducted for all exposure times, avoidance behavior assessed after 48 h and reproduction after 28d. Subcellular endpoints included enzymatic biomarker responses, namely, carboxylesterase, glutathione peroxidase, acetylcholinesterase, glutathione reductase, glutathione S-transferase and catalase activities, as well as fluorescence-based measurements of oxidative stress-related markers and multixenobiotic resistance activity. Multiple biomarkers showed significant changes in activity, but a recovery of most enzymatic activities could be observed after 28d. Overall, only minor effects could be observed on a subcellular level, showing that in this exposure scenario with environmentally relevant concentrations based on German pollution levels the threat to soil biota is minimal. However, in areas with higher concentrations of microplastics in the environment, these results can be interpreted as an early warning signal for more adverse effects. In conclusion, these findings provide new insights regarding the ecotoxicological effects of environmentally relevant concentrations of microplastics on soil organisms.

Simple summary Protocols were adjusted and established for biomarker assessment in two blood fractions: plasma and the post-mitochondrial fraction (S9). Basal biomarker values were determined in white stork (Ciconia ciconia) nestlings from Croatia, and biomarker responses in two different types of blood samples were compared. Novel fluorescence-based glutathione and reactive oxygen species detection was established providing the potential usage of blood for assessment of environmental impact at the molecular level. Abstract White stork (Ciconia ciconia) nestlings can provide quantitative information on the quality of the surrounding environment by indicating the presence of pollutants, as they depend on locally foraged food. This study represents the first comparison of biomarkers in two fractions of white stork nestling blood: plasma and S9 (the post-mitochondrial fraction). The aim of this study was to evaluate acetylcholinesterase (AChE), carboxylesterase (CES), glutathione S-transferase (GST), and glutathione reductase (GR), as well as to establish a novel fluorescence-based method for glutathione (GSH) and reactive oxygen species (ROS) detection in plasma and S9. Considering the enzymatic biomarkers, lower variability in plasma was detected only for AChE, as CES, GST, and GR had lower variability in S9. Enzyme activity was higher in plasma for AChE, CES, and GST, while GR had higher activity in S9. Regarding the fluorescence-based method, lower variability was detected in plasma for GSH and ROS, although higher GSH detection was reported in S9, and higher ROS was detected in plasma. The present study indicated valuable differences by successfully establishing protocols for biomarker measurement in plasma and S9 based on variability, enzyme activity, and fluorescence. For a better understanding of the environmental effects on nestlings’ physiological condition, biomarkers can be measured in plasma and S9.

The usage of pesticides has been steadily increasing over the last decades, and among them herbicides are the most commonly used ones. Despite their main mode of action targeting plant organisms, they can also have adverse effects on non-target animal organisms. In soil ecosystems, earthworms play an important role due to their positive impacts on the soil functioning and they represent good model organisms in soil ecotoxicology. The aim of the present study was to assess the effects of two herbicides on several endpoints at different levels of biological organization in the earthworm Eisenia andrei. Diuron and fluazifop-p-butyl were selected for the investigation and their lethal concentrations were determined: LC50 48 h: 89.087 μg/cm2 for diuron and 6.167 μg/cm2 for fluazifop-p-butyl. Furthermore, measurements of enzymatic biomarkers (catalase (CAT), acetylcholinesterase (AChE), carboxylesterase (CES) and glutathione S-transferase (GST)), multixenobiotic resistance (MXR) activity and gene expression of antioxidative enzymes (only for fluazifop-p-butyl) were conducted. Enzymatic biomarker responses showed no significant differences compared to the control after the exposure to the investigated herbicides, whereas the MXR activity was significantly inhibited. The gene expression level of superoxide dismutase (sod) and glutathione S-transferase (gst) after fluazifop-p-butyl exposure showed a significant increase. Finally, avoidance behavior in soil was assessed and it was determined that both herbicides caused significant avoidance response. The obtained results show that both investigated herbicides significantly affect earthworms on different levels of biological organization. This emphasizes the importance of comprehensive ecotoxicological assessment of herbicide effects on non-target organisms at all organizational levels.

Green toxicology is a novel approach increasingly applied for the development of materials and chemicals that are more benign to the environment and human health than their conventional counterparts. It includes predictive eco-toxicological assessments of chemicals during the early developmental process to exclude adverse effects. In the present study, two guanidine zinc catalysts for the ring-opening polymerization of lactide were investigated using eco-toxicological tools. Namely, the fish embryo toxicity assay for teratogenic effects, the ER (α) CALUX assay for endocrine activity and the Ames fluctuation assay for mutagenic potential were applied. Both complexes showed no endocrine activity, mutagenicity or acute aquatic toxicity, however a delayed hatch could be observed, therefore suggesting potential effects on a molecular level. This proof-of-concept study aims to assess the toxicity of guanidine zinc catalysts and is a first step towards the incorporation of toxicological assessments into chemical developmental processes to achieve a sustainable and safe production of catalysts.

Background Zebrafish have been increasingly used for monitoring and assessing the effects of different contaminants in the aquatic environments. In the present study, zebrafish embryos and larvae were used to study the effects of the insecticide diazinon and the herbicide diuron in regard to occurrence of oxidative stress-related cellular responses, multixenobiotic resistance (MXR)-related efflux transporter activity—which represents the first line of defense against xenobiotics in many aquatic organisms—and responses of different molecular and biochemical biomarkers. Results The recently established non-invasive quantitative plate assay, which uses the fluorescent probes CM-H2DCFDA and CellTracker™ Green CMFDA and measures the fluorescence in whole zebrafish larvae, was applied to assess changes in reactive oxidative species (ROS) and glutathione (GSH) after exposure to the investigated pesticides. The results showed a significant increase of GSH after 1h exposure of zebrafish larvae to both diazinon and diuron. Regarding the ROS induction, no significant increases in fluorescence could be detected after 2h exposure to the investigated pesticides. Applying a newly adapted assay for MXR activity, it was determined that diuron caused no change after a 24-h exposure, but caused a significant induction of MXR activity after a 48-h exposure (indicated by a decreased amount of accumulated rhodamine B). On the other hand, diazinon caused an inhibition of MXR activity after both 24h and 48h exposure (indicated by an increased amount of accumulated rhodamine B). Regarding the biomarkers, different set-ups and exposure periods were applied and both molecular (gene expression) and biochemical (enzymatic activities) responses were assessed. Diazinon caused an inhibition of carboxylesterase (CES) and acetylcholinesterase (AChE) activity in zebrafish larvae, diuron inhibited AChE activity in in vitro testing, and both pesticides significantly affected gene expression and activities of some of the cytochrome P450 (CYP) family enzymes. Conclusions The obtained results show various effects of the investigated pesticides and will help to elucidate how aquatic animals cope with pesticides present in their environment. Additionally, the recently developed fluorescence-based assay and the newly adapted MXR activity assay proved to be useful tools for ecotoxicological risk assessment to further investigate pesticide effects.

This study presents a high-throughput (HTP) micronucleus assay in multi-well plates with an automated evaluation for risk assessment applications. The evaluation of genotoxicity via the micronucleus assays according to international guidelines ISO 21427-2 with Chinese hamster (Cricetulus griseus) V79 cells was the starting point to develop our methodology. A drawback of this assay is that it is very time consuming and cost intensive. Our HTP micronucleus assay in a 48-well plate format allows for the simultaneous assessment of five different sample-concentrations with additional positive, negative and solvent controls with six technical replicates each within a quarter of the time required for the equivalent evaluation using the traditional slide method. In accordance with the 3R principle, animal compounds should be replaced with animal-free alternatives. However, traditional cell culture-based methods still require animal derived compounds like rat-liver derived S9-fraction, which is used to simulate the mammalian metabolism in in vitro assays that do show intrinsic metabolization capabilities. In the present study, a recently developed animal-free biotechnological alternative (ewoS9R) was investigated in the new high-throughput micronucleus assay. In total, 12 different mutagenic or genotoxic chemicals were investigated to assess the potential use of the animal-free metabolization system (ewoS9R) in comparison to a common rat-derived product. Out of the 12 compounds, one compound did not induce micronuclei in any treatment and 2 substances showed a genotoxic potential without the need for a metabolization system. EwoS9R demonstrated promising potential for future applications as it shows comparable results to the rat-derived S9 for 6 of the 9 pro-genotoxic substances tested. The remaining 3 substances (2-Acetamidofluorene, Benzo[a]pyrene, Cyclophosphamide) were only metabolized by rat-derived S9. A potential explanation is that ewoS9R was investigated with an approx. 10-fold lower enzyme concentration and was only optimized for CYP1A metabolization that may be improved with a modified production procedure. Future applications of ewoS9R go beyond the micronucleus assay, but further research is necessary.

Microplastic pollution has drawn the attention of both scientists and the public regarding their potential ecotoxicological risks. In the present study, we carried out aqueous exposure experiments to adult zebrafish with polystyrene microplastics (5 μm) at a wide range of concentrations (0.001-20 mg/L, equals to 14.5∼2.9 × 105 particles/mL). Our results showed the gastrointestinal tract (GIT) was the dominant microplastic accumulation site in zebrafish, followed by the gill, whereas no microplastics were detected in the brain or muscle. Microplastic accumulation in GIT did not cause obvious damages to intestinal villi in general. However, the thickness of muscularis layer in the foregut reduced by 32% after 1 mg/L (1.45 × 104 particles/mL) microplastic exposure. As there were no signs of oxidative stress or other histological changes found in the fish, we further investigated the energy-supplying influential factors. We found that the zebrafish became hyperactive after microplastic exposure, whose swimming distance had increased to 1.3-2.4 folds than that of control, and also stayed at manic and active states much longer. The fish behavioural alteration is probably attributed to the particulate matter stimulation and the up-regulation of estrogen contents. Results also showed that the excessive movements of zebrafish also led to decreased glucose and acetaldehyde metabolite contents and increased amino acid amounts, which further proved the shortage of energy-supplying substances. Therefore, the present study suggests that micro-sized microplastics can induce obvious behavioural abnormality at concentrations that some other toxicological endpoints may not warn effects.

Decentralized drinking water purification complements water supply in areas with unreliable or absent infrastructure. The exacerbating consequences of climate change in form of droughts and floods force remote households to tap various water sources. Hence, household-based processes must be versatile to cope with e.g. contaminated ground water and turbid surface waters. Purification at household level must be self-sustaining in order to enable independence from continuous supply of power and consumables. In this study, we design a process accordingly and we prove its technical feasibility on pilot scale. The two-step process utilizes gravity-driven ultrafiltration and activated carbon adsorption to purify water, whereas the process regeneration is accomplished by combining Temperature Enhanced Backwash and Temperature Swing Adsorption to clean the membrane and adsorber, respectively. We obtained stable operation over > 40 days with a sustained flowrate of ∼ 5 L h − 1 and consistent product quality (turbidity ≤ 0.2 NTU ) for all relevant water matrices: synthetic ground water, river water and even secondary effluent. We achieved a high removal of the spiked model micropollutant amitrole, environmental endocrine disruptors and bulk dissolved organics of ∼ 93 % , > 65 % and ∼ 69 % , respectively, at the optimal water recovery for river water of ∼ 80 % . In-situ regeneration promises long-term, self-sufficient operation without exhaustion.