1. In vitro exposure to CPF affects bovine sperm epigenetic gene methylation pattern and the ability of sperm to support fertilization and embryo development
- Author
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Pallotta Maria Michela, Barbato Vincenza, Pinton Alain, Acloque Hervè, Gualtieri Roberto, Talevi Riccardo, Jammes Helene, Capriglione Teresa, Dipartimento di Biologia, Università degli studi di Napoli Federico II, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Université Paris Saclay (COmUE), Pallotta, MARIA MICHELA, Barbato, Vincenza, Pinton, Alain, Acloque, Hervè, Gualtieri, Roberto, Talevi, Riccardo, Jammes, Helene, and Capriglione, Teresa
- Subjects
Male ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,Insecticides ,[SDV]Life Sciences [q-bio] ,Embryonic Development ,DNA Fragmentation ,DNA Methylation ,Spermatozoa ,Bos taurus ,Chromatin ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,Pregnancy ,GTP-Binding Protein alpha Subunits, Gs ,Sperm Motility ,Animals ,[INFO]Computer Science [cs] ,Cattle ,Female ,RNA, Long Noncoding ,Chlorpyrifos ,infertility ,Promoter Regions, Genetic ,Spermatogenesis ,Xist gene ,organophosphate pesticides - Abstract
Several studies have demonstrated that overexposure to pesticides can reduce mammalian sperm quality, impairing male fertility. Chlorpyrifos (CPF), a widely used organophosphate pesticide, was shown to impair spermatogenesis by inducing the formation of highly reactive toxic intermediates. To gain further insight into the mechanisms underlying the cytotoxicity and genotoxicity of CPF, bovine spermatozoa were exposed in vitro to environmental CPF concentrations and the motility, in vitro fertilization rates, DNA fragmentation, chromatin alterations, and methylation patterns were assessed. Motility and in vitro fertilization rates were significantly reduced in spermatozoa exposed to CPF, while DNA fragmentation and putative chromatin deconstruction appeared to increase at higher pesticide concentrations. In situ hybridization was carried out with X and Y probes on sperm samples exposed to different CPF concentrations, and subsequent analysis highlighted a significant percentage of spermatozoa with a peculiar morphological malformation, in which a narrowing occurred at the level of the hybridization. Analysis of potential abnormalities in the methylation pattern of NESP55-GNAS and XIST promoters displayed no differentially methylated regions in GNAS promoter relative to the control, whereas spermatozoa exposed to 10 μg/mL CPF had increased methylation variance in one region of imprinted XIST promoter. Our results provide support that CPF can induce a genotoxic effect on spermatozoa, impairig their ability to fertilize and support preimplantation embryo development in vitro. These observations are worrying since altered levels of sporadic methylation in genes of male gametes may affect the success of reproduction and contribute to infertility. Environ. Mol. Mutagen. 60:85-95, 2019. © 2018 Wiley Periodicals, Inc.
- Published
- 2018
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