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31 results on '"Campa M"'

4. Antimicrobial activity of human hepcidin 20 and 25 against clinically relevant bacterial strains: effect of copper and acidic pH

Author

Maisetta G 1, Petruzzelli R 2, Brancatisano FL 1, Esin S 1, Vitali A 3, Campa M 1, and Batoni G 1

Subjects
hepcidin 25, pH, Antimicrobial peptides, hepcidin 20
Abstract

Hepcidin 25 (hep-25) is a peptide primarily produced by human liver with a central role in iron homeostasis. Its isoform, hepcidin 20 (hep-20), has an unknown function and lacks the first five aminoacids of the amino-terminal portion. This sequence is crucial for iron regulation by hep-25 and contains a molecular motif able to bind metals. Aim of this study, was to evaluate the antibacterial properties of both peptides in vitro, against a wide range of bacterial clinical isolates and in different experimental conditions. Although both peptides were found to be bactericidal against a variety of clinical isolates with different antibiotic resistance profiles, hep-20 was active at lower concentrations than hep-25, in most of the cases. Killing kinetics, carried on in sodium-phosphate buffer at pH 7.4, demonstrated that bactericidal activity occurred not earlier than 30-90 min of incubation. Bactericidal activity of hep-25 was slightly enhanced in the presence of copper, while the same metal did not affect the activity of hep-20. Interestingly, bactericidal activity of both hepcidins was highly enhanced at acidic pH. Acidic pH (pH 5.0 and 6.6) not only reduced the microbicidal concentrations of hepcidins, but also shortened the killing times of both peptides, as compared to pH 7.4. Combining hep-20 and hep-25 at pH 5.0 a bactericidal effect could be obtained at very low concentrations of both peptides. These results render hepcidins interesting for the design of new drugs for the treatment of infections occurring in body districts with physiologic acidic pH.

Published
2010

19. Cisgenic grapevine: an ever closer goal

Author

Lorenza Dalla Costa, Stefano Piazza, Campa, M., Poletti, V., and Mickael Malnoy

Subjects
Cisgenesis, Settore BIO/11 - BIOLOGIA MOLECOLARE, Selection marker gene removal, Heat-shock inducible system

24. Protein extraction from grape tissues by two-dimensional electrophoresis

Author

Marsoni, M., CANDIDA VANNINI, Campa, M., Cucchi, U., Espen, L., and Bracale, M.

Subjects
IDENTIFICATION, RESOLUTION, PLANT-PROTEINS, GEL-ELECTROPHORESIS, SEPARATION, PATTERNS, PROTEOMIC ANALYSIS, MASS-SPECTROMETRY, DATABASES, TWO-DIMENSIONAL ELECTROPHORESIS, TWO-DIMENSIONAL ELECTROPHORESIS, GEL-ELECTROPHORESIS, PROTEOMIC ANALYSIS, MASS-SPECTROMETRY, PLANT-PROTEINS, IDENTIFICATION, SEPARATION, RESOLUTION, DATABASES, PATTERNS
Abstract

At the onset of proteomic studies protein samples have to be accurately separated by two dimensional electrophoresis (2-DE); subsequently polypeptides are identified. Grape tissues, in particular roots, can be very problematic due to their hardness and to the high content of compounds that interfere in classical protein extraction. We have used a phenol-based extraction method in the presence of a protease inhibitor and Polyvinylpolypyrrolidone (PVPP). In this paper we demonstrate that this extraction method gives satisfactory and reproducible protein separation allowing the identification of some proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)., VITIS - Journal of Grapevine Research, Vol. 44 No. 4 (2005): Vitis

30. Iron species in FeOx/ZrO2 and FeOx/sulphated-ZrO2 catalysts

Author

Valerio Indovina, Daniela Pietrogiacomi, Maria Cristina Campa, Franco Pepe, Simonetta Tuti, comitato scientifico, Indovina, V., Campa, M. C., Pepe, F., Pietrogiacomi, D., Tuti ', S., Aldo Gamba, Carmine Colella, Salvatore Coluccia, Tuti, Simonetta, Valerio, Indovina, Pepe, Franco, Cristina CAMPA, M., and Daniela, Pietrogiacomi

Subjects
iron, feox, catalysts, Aqueous solution, Chemistry, Inorganic chemistry, Infrared spectroscopy, Selective catalytic reduction, Toluene, FeOx/ZrO2 and FeOx/sulphated-ZrO2 catalysts, iron characterization, Catalysis, law.invention, chemistry.chemical_compound, Adsorption, FeOx/ZrO2, FeOx/sulphated-ZrO2 catalysts, FTIR, characterization, law, Calcination, Temperature-programmed reduction
Abstract

Iron containing catalysts were prepared by (i) impregnation of ZrO 2 with aqueous solutions of Fe(NO 3 ) 3 , Fe/Z i , or aqueous solutions of NH 4 Fe(SO 4 ) 2 , FeS/Z; (ii) chemical vapour deposition of FeCl 3 on ZrO 2 , Fe/Z CVD ; and (iii) impregnation of sulphated-ZrO 2 with toluene solutions of Fe(acetylacetonate) 3 , Fe/SZ. After drying at 383 K and calcining at 823 K, samples were characterized by temperature programmed reduction with H 2 and Fourier transformed IR spectroscopy. The FTIR characterization by CO adsorption evidenced the formation of various carbonyl-like species onto Fe n + sites (0 ≤ n ≤ 3). The relative amount of the various iron carbonyl-like species depended not on the iron content, but on the catalyst preparation method. The H 2 -TPR experiments showed that iron species were much less reducible in FeO x /sulphated-ZrO 2 than in FeO x /ZrO 2 . Their lower reducibility explains why FeO x /sulphated-ZrO 2 samples achieve higher selectivity for the selective catalytic reduction of NO with hydrocarbons in the presence of O 2 .

Published
2005
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