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The aim of this study was to investigate the correlation and predictive value of serum miR-146b-5p expression during the first trimester of pregnancy with pre-eclampsia (PE). In total, 32 normal pregnant women (the control group) and 58 subjects with PE were randomly selected from eligible case data. The serum levels of miR-146b-5p, pregnancy associated plasma protein A (PAPP-A) and free beta subunit of human chorionic gonadotropin (free β-hCG) were then detected. Next, we established predictive models of single or multiple markers for PE. The levels of miR-146b-5p in the mild pre-eclampsia (mPE) and severe pre-eclampsia (sPE) groups were higher than the control group and there were significant differences between the three groups (

Background: To study whether ILs/TNFs in the follicular fluid (FF) of women with EMs are responsible for impaired follicular development or (and) ovulation or not, and then to explore the underlying mechanisms. Methods: follicular fluid (containing cumulus granulosa cells) was collected from women with EM and male factor infertility at our Clinical Reproductive Medicine Center, and peritoneal fluid was collected from the above patients with EMs. The expression of ovulation-related genes in cumulus cells was analysed by RT-PCR. Mouse cumulus cells expansion degree was assessed after cultured in follicle fluid from infertile women. Follicle fluid was detected by ELISA. Oocytectmized complex cell model was established, and cultured in vitro medium with addition of 100 IU/ml FSH. TUNEL staining was used to determine the apoptosis of cumulus cells. Then, we explored expression of P-SMAD2/3，key enzyme for retinoic acid metabolism, and methylation of SP1 binding sites in Lhcgr promoter region. Meanwhile, the P-AKT and P-catenin were assessed by Western blot. All experiments were performed independently at least three times, and data are presented as mean ± SEM. Statistical analyses were performed using Graphpad Prism 5 software pResults: In cumulus cells, expression of genes related to ovulation decreased significantly than that in controls (P < 0.05), especially starting from LHCGR. The concentrations of IL-8 and TNF-α in follicle fluid were significantly higher in infertile women with endometriosis than in controls (P < 0.05). The function of follicle fluid and pelvic fluid of endometriosis women have changed. Addition of 500 pg/mL IL-8/TNF-α to medium did not cause significant apoptosis of cumulus cells, but inhibited P-AKT and P-β-catenin. On the other hand, expression of P-SMAD2/3 and retinoic acid production were reduced, while hypermethylation of the Sp1 binding sequence on Lhcgr promoter was identified, and Lhcgr expression was significantly reduced compared to control (PConclusion: Elevated IL-8/TNF-α in follicular fluid of women with endometriosis indirectly maintains Lhcgrpromoter hypermethylation through activation of P-SMAD2/3, while inhibiting AKT and β-Catenin phosphorylation, which together reduce LHCGR mRNA expression.

To investigate the effects of down's screening markers and maternal characteristics on preeclampsia (PE) pregnancy outcome during early and middle pregnancy.A retrospective study of a cohort of 246 PE and 18,709 No-PE pregnant women who participated in Down's screening during early and middle pregnancy was performed. Clinical data of pregnancy-related were collected. Multivariate binary logistic regression was used to analyze the adjusted odds ratio (aOR) and 95% confidence interval (CI) of Down's screening markers, maternal characteristics, pregnancy outcome, and other related variables, and to evaluate the influencing factors of each indicator on PE.Compared with the non-PE group, the concentration and median multiple (MoM) of pregnancy-associated plasma protein-A (PAPP-A) and free beta subunit of human chorionic gonadotropin (free β-hCG) in PE group were both lower (Decreased maternal serum PAPP-A level, increased free β-hCG, hyperlipemia, premature delivery, cesarean section, live birth, hydronephrosis, fetal growth retardation, low birth weight, and infection are risk factors for PE, while uterine scar and premature rupture of membrane are protective factors for PE.

Background This study analyzed the pregnancy outcomes of patients with intrahepatic cholestasis of pregnancy (ICP) in Hangzhou, China. Methods Cases of pregnant women monitored by antepartum testing at Hangzhou Women’s Hospital from January 2018 to December 2020 were reviewed. Subjects were classified into two groups according to whether they had ICP: 688 cases of ICP were assigned to an exposure group while 38,556 cases of non-ICP were assigned to a non-exposed group. Univariate analysis was performed on qualitative or quantitative data using the Chi-Squared test or Mann–Whitney U test, and the adjusted odds ratio (aOR) and 95% confidence interval (CI) of the two groups of related variables were calculated by multivariate binary logistic regression analysis. Results The incidence rate of ICP was 1.75%. Pregnant women with hepatitis B virus were correlated with ICP. Hepatitis B carriers (aOR = 3.873), preeclampsia (PE, aOR = 3.712), thrombocytopenia (aOR = 1.992), gestational hypertension (GH, aOR = 1.627), hyperlipidemia (aOR = 1.602) and gestational diabetes mellitus (GDM, aOR = 1.265) were all risk factors for ICP. In contrast, Body Mass Index (BMI) ≥ 30 kg/m2 (aOR = 0.446), 25 m2 2 (aOR = 0.699) and parity ≥ 1 (aOR = 0.722) were protective factors for ICP. Pregnant women in the ICP group had an increased risk of gestation days Conclusions By analyzing a Chinese population with ICP, we identified that pregnant women who are hepatitis B carriers or with PE, thrombocytopenia, GH, hyperlipidemia, and GDM are at higher risk of ICP. Moreover, ICP is associated with adverse pregnancy outcomes; in particular, ICP may increase the incidence of shorter gestational days and non-vaginal delivery methods such as cesarean section but reduce the incidence of premature rupture of membranes and fetal macrosomia.

We aimed to investigate the predictive ability of serum levels of D-dimer (DD) in the first trimester for the occurrence of hypertensive disorders of pregnancy (HDP). In this retrospective, case-cohort study, we measured the levels of DD, plasma pregnancy-associated protein A (PAPP-A), and free

This paper aimed to investigate the characteristics of female HPV infection in the Shangcheng District, Hangzhou city, China. The retrospective study was designed to analyze the HPV prevalence rate of 22,382 women receiving physical examinations from 2016 to 2020 in the Shangcheng District of Hangzhou city in China. A commercial kit was designed to detect the HPV genotypes. Trends were examined for age-specific groups (≤ 30 years, 31–44 years, 45–54 years, 55–64 years, ≥ 65 years). A receiver operating characteristic (ROC) analysis was used to assess the correlation of age classification in high risk HPV (HR-HPV) infection. 22.41% (5015/22,382) of samples were HPV positive, 91.28% (4578/5015) of HPV positive women were infected by HR-HPV. The most prevalent HR-HPV genotypes were 16, 52, 18, 58, 56, and 51. The trend of HPV prevalence showed the significant differences in age-specific groups (χ2 = 164.70, P