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Li Wang, Na Zhao, Li Zhou, Juan Tong, Lei Xue, Lei Zhang, Yongsheng Han, Xingbing Wang, Liangquan Geng, Baolin Tang, Huilan Liu, Weibo Zhu, Xiaoyan Cai, Xin Liu, Xiaoyu Zhu, Zimin Sun, Changcheng Zheng Department of Hematology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, People’s Republic of ChinaCorrespondence: Changcheng ZhengDepartment of Hematology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Lujiang Road No. 17, Hefei, 230001, People’s Republic of ChinaTel/Fax +86-551-62284476Email zhengchch1123@ustc.edu.cnBackground: There is great uncertainty in the treatment of elderly patients with acute myeloid leukemia (AML), which leads to great challenges in treatment decision. The aim of this study is to find more suitable induction therapy and consolidation therapy for elderly AML patients.Methods: A total of 149 consecutive newly diagnosed elderly AML patients (aged ≥ 60 years) who received induction chemotherapy in our medical center from January 2015 to December 2019 were retrospectively analyzed.Results: After the first induction treatment, the complete remission/or complete remission with incomplete hematologic recovery (CR/CRi) rates in the standard-intensity chemotherapy group was significantly higher than that in the low-intensity chemotherapy group (58.2% vs 32.9%, p = 0.003). Compared with the low-intensity chemotherapy, the incidence of severe infection in the standard-intensity chemotherapy was significantly increased (p < 0.001), but the early mortality was comparable. One hundred and seven patients received minimal residual disease (MRD) examination after the first induction treatment; and MRD was negative accounting for 51.9% in the standard-intensity chemotherapy group, while only 32.7% in the low-intensity group (p = 0.05). The 2-year-overall survival (OS) of patients in standard-intensity induction chemotherapy group (37.2%) was slightly higher than that in low-intensity induction chemotherapy group (23.4%) (p = 0.075). Eighty-one CR/CRi patients received intermediate or high dose cytarabine (n = 35) or sequential chemotherapy regimens (n = 46) as consolidation treatment. The 2-year OS and event-free survival (EFS) of patients in the intermediate or high-dose cytarabine group were significantly higher than those in the sequential chemotherapy regimens group (73.0% vs 38.5%, p = 0.002; 54.8% vs 35.0%, p = 0.035).Conclusion: Our results showed that standard-intensity induction chemotherapy can significantly improve the CR rate for elderly AML patients, and does not increase the early mortality; consolidation therapy with intermediate or high-dose cytarabine can significantly improve EFS and OS for elderly AML patients achieved CR.Keywords: acute myeloid leukemia, elderly patients, minimal residual disease, standard-intensity chemotherapy, intermediate or high dose cytarabine

Supplementary Material 1

Allotetraploid cotton ( Gossypium ) species represents a model system for the study of plant polyploidy, molecular evolution, and domestication. Here, chromosome-scale genome sequences were obtained and assembled for two recently described wild species of tetraploid cotton, Gossypium ekmanianum [(AD) 6 , Ge ] and Gossypium stephensii [(AD) 7 , Gs ], and one early form of domesticated Gossypium hirsutum , race punctatum [(AD) 1 , Ghp ]. Based on phylogenomic analysis, we provide a dated whole-genome level perspective for the evolution of the tetraploid Gossypium clade and resolved the evolutionary relationships of Gs , Ge , and domesticated G. hirsutum . We describe genomic structural variation that arose during Gossypium evolution and describe its correlates—including phenotypic differentiation, genetic isolation, and genetic convergence—that contributed to cotton biodiversity and cotton domestication. Presence/absence variation is prominent in causing cotton genomic structural variations. A presence/absence variation-derived gene encoding a phosphopeptide-binding protein is implicated in increasing fiber length during cotton domestication. The relatively unimproved Ghp offers the potential for gene discovery related to adaptation to environmental challenges. Expanded gene families enoyl-CoA δ isomerase 3 and RAP2-7 may have contributed to abiotic stress tolerance, possibly by targeting plant hormone-associated biochemical pathways. Our results generate a genomic context for a better understanding of cotton evolution and for agriculture.

Supplemental material, sj-docx-1-taj-10.1177_20406223221119626 for Association between non-alcoholic fatty liver disease and risk of incident heart failure: a meta-analysis of observational studies by Wensheng Li, Weixing Wen, Dongxiao Xie, Min Qiu, Xiaoyan Cai, Sulin Zheng and Yuli Huang in Therapeutic Advances in Chronic Disease

Additional file 2: Supplementary Figure S2. Gene ontology (GO) annotation classification. A, Biological function for G. hirsutum B, Molecular function of G. hirsutum C, Biological function for G. arboreum D, Molecular function of G. arboreum E, Biological function for G. raimondii F, Molecular function of G. raimondii, there is no significant classification for cellular function, the logo stands for the level of significance.

Supplemental material, sj-docx-1-taj-10.1177_20406223221119626 for Association between non-alcoholic fatty liver disease and risk of incident heart failure: a meta-analysis of observational studies by Wensheng Li, Weixing Wen, Dongxiao Xie, Min Qiu, Xiaoyan Cai, Sulin Zheng and Yuli Huang in Therapeutic Advances in Chronic Disease

Additional file 3: Supplementary Figure S3. Phylogenetic relationships of gene structure analysis and Motif Identification in Gossypium species of NAC genes. A, G. hirsutum, B, G. arboreum, C, G. raimondii.

Additional file 1: Supplementary Figure S1. Chromosomal locations of NAC genes in three cotton species. The chromosomal position of cotton Species were mapped inline to their genome. A, G. hirsutum with At subgenome B, G. hirsutum with Dt subgenome C, G. arboreum D, G. raimondii E, Scaffold collections.

Additional file 4: Supplementary Figure S4. PCR amplification and gel band formation of the 1038bp coding sequence gene Gh_D01G0514 (GhNAC072) using 5000bp marker.

Supplemental material, sj-pdf-1-taj-10.1177_20406223211024361 for Non-alcoholic fatty liver disease is associated with increased risk of chronic kidney disease by Xiaoyan Cai, Lichang Sun, Xiong Liu, Hailan Zhu, Yang Zhang, Sulin Zheng and Yuli Huang in Therapeutic Advances in Chronic Disease

Additional file 7. The interaction network for CDK genes constructed by Cytoscape.

Additional file 6 Conserved domain and motifs of hybrid-Bos taurus CDK proteins.

Additional file 3. Conserved domain prediction of bovine CDK protein sequences.

Additional file 4. Amino acid sequences logos of 10 identified motifs in bovine CDK proteins.

Supplemental material, sj-pdf-1-taj-10.1177_20406223211024361 for Non-alcoholic fatty liver disease is associated with increased risk of chronic kidney disease by Xiaoyan Cai, Lichang Sun, Xiong Liu, Hailan Zhu, Yang Zhang, Sulin Zheng and Yuli Huang in Therapeutic Advances in Chronic Disease

Additional file 5. Oil Red O staining of preadipocytes and differentiated adipocytes.

Segregation distortion (SD) is a phenomenon common among stable or segregating populations, and the principle behind it is still an issue that puzzles many researchers. An F2:3 generations developed from the wild cotton species of the D genomes was applied to investigate the possible plant transcription factors within the segregation distortion regions (SDRs). We constructed a consensus map between two maps in the D genome, map A; Gossypium klotzschianum and Gossypium davidsonii and Map B; Gossypium thurberi and Gossypium trilobum. The two maps were developed from 188 F2:3 populations for each map, a total of 1492 markers, were linked to the 13 linkage groups. The consensus linkage map size was 1467.445 cM with an average marker distance of 1.0370cM. Chr02 had the highest percentage of segregation distortion with 58.621% followed by Chr07 with 47.887%. A total of 6,038 genes were mined within the segregation distortion regions (SDR region) of Chr02 and Chr07 with 2,308 gene in Chr02 and 3,730 genes in Chr07, we obtained a total of 1,117 domains within the SDR with a total of 622 domains shared between the two chromosomes, the first 9 domains all belonged to the plant resistance genes (R genes), the largest domain was PF00069 with a total of 188 genes. A total of 287 miRNAs were found to target the various genes, such as gr-miR398, gra-miR5207, miR164a, miR164b, miR164c among others which have been found to target top-ranked stress-responsive transcription factors such as NAC genes. Moreover, the genes were found to be regulated by various stress responsive cis-regulatory elements. RNA profiling showed that higher numbers of genes were highly upregulated in abiotic and different fiber development stages. The result shows that the SDR regions could be playing an important role in the evolution of significant genes in plants.

Summary The diploid wild cotton species Gossypium australe possesses excellent traits including resistance to disease and delayed gland morphogenesis, and has been successfully used for distant breeding programmes to incorporate disease resistance traits into domesticated cotton. Here, we sequenced the G. australe genome by integrating PacBio, Illumina short read, BioNano (DLS) and Hi‐C technologies, and acquired a high‐quality reference genome with a contig N50 of 1.83 Mb and a scaffold N50 of 143.60 Mb. We found that 73.5% of the G. australe genome is composed of various repeat sequences, differing from those of G. arboreum (85.39%), G. hirsutum (69.86%) and G. barbadense (69.83%). The G. australe genome showed closer collinear relationships with the genome of G. arboreum than G. raimondii and has undergone less extensive genome reorganization than the G. arboreum genome. Selection signature and transcriptomics analyses implicated multiple genes in disease resistance responses, including GauCCD7 and GauCBP1, and experiments revealed induction of both genes by Verticillium dahliae and by the plant hormones strigolactone (GR24), salicylic acid (SA) and methyl jasmonate (MeJA). Experiments using a Verticillium‐resistant domesticated G. barbadense cultivar confirmed that knockdown of the homologues of these genes caused a significant reduction in resistance against Verticillium dahliae. Moreover, knockdown of a newly identified gland‐associated gene GauGRAS1 caused a glandless phenotype in partial tissues using G. australe. The G. australe genome represents a valuable resource for cotton research and distant relative breeding as well as for understanding the evolutionary history of crop genomes.

Automatic abstractive summary generation is still an open problem in natural language processing field. Conventional encoder–decoder model based abstractive summarization methods often suffer from repetition and semantic irrelevance. Recent studies apply traditional attention or graph-based attention on the encoder–decoder model to tackle the problem, under the assumption that all the sentences in the original document are indistinguishable from each other. But in a document, the same words in different sentences are not equally important, i.e., the words in a trivial sentence are less important than the words in a salient sentence. Based on it, we develop a HITS-based attention mechanism in this paper, which fully leverages sentence-level and word-level information by considering sentences and words in the original document as authorities and hubs. Based on it, we present a novel abstractive summarization method, with Kullback–Leibler (KL) divergence to refine the attention value, meanwhile we propose a comparison mechanism in summary generation to further improve the summarization performance. When evaluated on the CNN/Daily Mail and NYT datasets, the experimental results demonstrate the improvement of summarization performance and show the performance of our proposed method is comparable with that of the other summarization methods. Besides, we also conduct experiments on CORD-19 dataset (COVID-19 Open Research Dataset) which is a biomedical domain dataset, and the experimental results show great performance of our proposed method compared with that of the other state-of-the-art summarization methods.

Background Chromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton (Gossypium Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding. Results Using the linker adaptor PCR (LA-PCR) with the primers of rice disease-resistance homologues, three nucleotide sequences PS016 (KU051681), PS054 (KU051682), and PS157 (KU051680) were obtained from the chromosome Ah01 of upland cotton (cv. TM-1). The Blast results showed that the three sequences are the nucleotide binding site-leucine rich repeat (NBS-LRR) type RGAs. Clustering results indicated that they are homologous to these published RGAs. Thus, the three RGAs can definitely be confirmed as NBS-LRR class of RGAs in upland cotton. Conclusions Using single chromosome microdissection technique, DNA libraries containing cotton RGAs were obtained. This technique can promote cotton gene cloning, marker development and even the improvement of cotton genome research and breeding.

Most existing algorithms on spectral clustering are not able to determine the number of clusters. In this paper, we prove theoretically that the eigenvectors of the affinity matrix can be used directly to cluster the data points. And we suggest exploiting the structure of the eigenvectors to infer automatically the number of clusters. As a result, a self-adaptive spectral clustering algorithm based on affinity matrix is proposed. The experimental results on the UCI data sets show that the algorithm is more effective than previous algorithms.

As an application of mobile ad hoc networks in transportation realm, Vehicular Ad hoc Network (VANET) is a prominent technology for inter-vehicle communications in Intelligent Transportation System. City environment is a main scenario for VANET applications, and routing for urban VANET faces greater challenges because of potential more uneven distribution of vehicular nodes, constrained mobility pattern and difficult signal reception due to radio obstacles. This paper investigates and compares several routing algorithms designed for urban VANET. A qualitative comparison of these approaches is provided as well as some opportunities for future research. The goals and key elements of a well-performed routing scheme are also proposed to help designing a solution to meet city scenarios.

Sentence clustering plays a pivotal role in theme-based summarization, which discovers topic themes defined as the clusters of highly related sentences in order to avoid redundancy and cover more diverse information. As the length of sentences is short and the content it contains is limited, the bag-of-words cosine similarity traditionally used for document clustering is no longer reasonably suitable. Special treatment for measuring sentence similarity is necessary. In this paper, we propose a ranking-based clustering framework that utilizes ranking distribution of documents and terms to help generate high quality sentence clusters. The effectiveness of the proposed framework is demonstrated by both the cluster quality analysis and the summarization evaluation conducted on the DUC 2004 and DUC2007 datasets. Refereed/Peer-reviewed