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3 results on '"Bruker BioSpin MRI GmbH [Ettlingen, Germany]"'

1. Detection of Metabolite–Protein Interactions in Complex Biological Samples by High-Resolution Relaxometry: Toward Interactomics by NMR

Author

Jean-Max Tyburn, Simone Pisano, Ziqing Wang, Veronica Ghini, Philippe Pelupessy, Morgan Kazmierczak, Giacomo Parigi, Thorsten Marquardsen, Fabien Ferrage, Guillaume Bouvignies, Claudio Luchinat, Pavel Kadeřávek, Milan Zachrdla, Laboratoire des biomolécules (LBM UMR 7203), Chimie Moléculaire de Paris Centre (FR 2769), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (CIRMMP), Università degli Studi di Siena = University of Siena (UNISI)-Università degli Studi di Firenze = University of Florence (UniFI)-University of Bologna/Università di Bologna-Partenaires INRAE, Structure et Dynamique des Biomolécules (LBM-E3), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Chimie Moléculaire de Paris Centre (FR 2769), Ecole Polytechnique Fédérale de Lausanne (EPFL), Bruker BioSpin MRI GmbH [Ettlingen, Germany], Università degli Studi di Firenze = University of Florence (UniFI), Gestionnaire, HAL Sorbonne Université 5, Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Chimie Moléculaire de Paris Centre (FR 2769), Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli Studi di Siena = University of Siena (UNISI)-Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI)-University of Bologna-Partenaires INRAE, Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Département de Chimie - ENS Paris, and Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI)

Subjects
Relaxometry, Human blood, Chemistry, Metabolite, High resolution, General Chemistry, Computational biology, 010402 general chemistry, 01 natural sciences, Biochemistry, Small molecule, Catalysis, NMR, 0104 chemical sciences, Protein–protein interaction, chemistry.chemical_compound, Colloid and Surface Chemistry, Metabolomics, [CHIM] Chemical Sciences, [CHIM]Chemical Sciences, Interactomics, Macromolecule
Abstract

International audience; Metabolomics, the systematic investigation of metabolites in biological fluids, cells or tissues, reveals essential information about metabolism and diseases. Metabolites have functional roles in myriads of biological processes, as substrates and products of enzymatic reactions, but also as cofactors and regulators of large numbers of biochemical mechanisms. These functions involve interactions of metabolites with macromolecules. Yet, methods to systematically investigate these interactions are hitherto still scarce. In particular, there is a need for techniques suited to identify and characterize weak metabolite-macromolecule interactions directly in complex media such as biological fluids. Here, we introduce a method to investigate weak interactions between metabolites and macromolecules in biological fluids. Our approach is based on high-resolution NMR relaxometry and does not require any invasive procedure or separation step. We show that we can detect interactions between small and large molecules in human blood serum and quantify the size of the complex. Our work opens the way for investigations of metabolite-(or other small molecules) protein interactions in biological fluids for interactomics or pharmaceutical applications.

Published
2021
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2. Fast 3D ultrashort echo-time spiral projection imaging using golden-angle: A flexible protocol for in vivo mouse imaging at high magnetic field

Author

Eric Thiaudière, Jean-Michel Franconi, Emeline J. Ribot, Charles R. Castets, Sylvain Miraux, Aurélien J. Trotier, Didier Wecker, William Lefrançois, Centre de résonance magnétique des systèmes biologiques (CRMSB), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), and Bruker BioSpin MRI GmbH [Ettlingen, Germany]

Subjects
7T, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, self-gating, Ferric Compounds, Imaging phantom, Spiral, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, Magnetics, Mice, Motion, 0302 clinical medicine, Nuclear magnetic resonance, Imaging, Three-Dimensional, In vivo, Robustness (computer science), Image Interpretation, Computer-Assisted, medicine, Animals, Radiology, Nuclear Medicine and imaging, golden-angle, ComputingMilieux_MISCELLANEOUS, mouse, Physics, medicine.diagnostic_test, Phantoms, Imaging, Angiography, Brain, Magnetic resonance imaging, Image Enhancement, Magnetic Resonance Imaging, 3. Good health, Mice, Inbred C57BL, Liver, Undersampling, UTE, Ultrashort echo time, Golden angle, Artifacts, 030217 neurology & neurosurgery, 3D
Abstract

Purpose To develop a fast three-dimensional (3D) k-space encoding method based on spiral projection imaging (SPI) with an interleaved golden-angle approach and to validate this novel sequence on small animal models. Methods A disk-like trajectory, in which each disk contained spirals, was developed. The 3D encoding was performed by tilting the disks with a golden angle. The sharpness was first calculated at different T2* values. Then, the sharpness was measured on phantom using variable undersampling ratios. Finally, the sampling method was validated by whole brain time-of-flight angiography and ultrasmall superparamagnetic iron oxide (USPIO) enhanced free-breathing liver angiography on mouse. Results The in vitro results demonstrated the robustness of the method for short T2* and high undersampling ratios. In vivo experiments showed the ability to properly detect small vessels in the brain with an acquisition time shorter than 1 min. Free-breathing mice liver angiography showed the insensitivity of this protocol toward motions and flow artifacts, and enabled the visualization of liver motion during breathing. Conclusions The method implemented here allowed fast 3D k-space sampling with a high undersampling ratio. Combining the advantages of center-out spirals with the flexibility of the golden angle approach could have major implications for real-time imaging. Magn Reson Med 77:1831-1840, 2017. © 2016 International Society for Magnetic Resonance in Medicine.

Published
2016
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3. Selective Heart Rate Reduction Improves Metabolic Syndrome-related Left Ventricular Diastolic Dysfunction

Author

Nassiba Merabet, Vincent Richard, Christelle Monteil, Lionel Nicol, Yuehua Fang, Didier Wecker, Jérôme Roussel, Jean-Paul Henry, Christian Thuillez, Isabelle Remy-Jouet, Sabrina Le Bouter-Banon, Paul Mulder, Montreal Heart Institute - Institut de Cardiologie de Montréal, Pharmacologie des Dysfonctionnements Endotheliaux et Myocardiques, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Institute for Research and Innovation in Biomedicine (IRIB), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Aliments Bioprocédés Toxicologie Environnements (ABTE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Endothélium, valvulopathies et insuffisance cardiaque (EnVI), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bruker BioSpin MRI GmbH [Ettlingen, Germany], Institut de Recherches Internationales Servier [Suresnes] (IRIS), Institut Pasteur de Dakar, and Réseau International des Instituts Pasteur (RIIP)

Subjects
Cardiac function curve, Male, Cardiac output, medicine.medical_specialty, Heart Ventricles, medicine.disease_cause, Ion Channels, Ventricular Function, Left, Electrocardiography, Ventricular Dysfunction, Left, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Diastole, Heart Rate, Internal medicine, Heart rate, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], medicine, Animals, Mesenteric arteries, Pharmacology, Metabolic Syndrome, business.industry, diastolic function, Hemodynamics, Cardiovascular Agents, medicine.disease, Rats, Zucker, Oxidative Stress, medicine.anatomical_structure, heart rate reduction, Cardiology, Left ventricular diastolic dysfunction, Metabolic syndrome, Cardiology and Cardiovascular Medicine, business, Perfusion, Oxidative stress
Abstract

Background: Enhanced heart rate observed in metabolic syndrome (MS) contributes to the deterioration of left ventricular (LV) function via impaired LV filling and relaxation, increased myocardial O2 consumption, and reduced coronary perfusion. However, whether heart rate reduction (HRR) opposes LV dysfunction observed in MS is unknown. Methods: We assessed in Zucker fa/fa rats, a rat model of MS, the cardiovascular effects of HRR induced by the If current inhibitor S38844 (3 mg·kg−1·d−1). Results: Delayed short-term (4 days) and long-term (90 days) HRR induced by S38844 reduced LV end-diastolic pressure and LV end-diastolic pressure–volume relation, increased myocardial tissue perfusion, decreased myocardial oxidized glutathione levels, and preserved cardiac output, without modifying LV end-systolic pressure and LV end-systolic pressure–volume relation, although only long-term S38844 opposed LV collagen accumulation. Long-term S38844 improved flow-induced endothelium-dependent dilatation of mesenteric arteries, while metabolic parameters, such as plasma glucose levels, and Hb1c, were never modified. Conclusions: In rats with MS, HRR induced by the If inhibitor S38844 improved LV diastolic function and endothelium-dependent vascular dilatation, independent from modifications in metabolic status. Moreover, this improvement in cardiac function involves not only immediate effects such as improved myocardial perfusion and reduced oxidative stress but also long-term effects such as modifications in the myocardial structure.

Published
2015
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