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Fluoxetine is one of the worlds most prescribed antidepressant, and frequently detected in surface waters. Once present in the aquatic environment, fluoxetine has been shown to disrupt the swimming behaviour of fish and invertebrates. However, swimming behaviour is also known to be highly variable according to experimental conditions, potentially concealing relevant effects. Therefore, the aims of this study were two-fold: i) investigate the swimming and feeding behaviour of Gammarus pulex after exposure to the antidepressant fluoxetine (0.2, 2, 20, and 200 mu g/L), and ii) assess to what degree the experimental test duration (short-term and long-term) and test location (laboratory and semi-field conditions) affect gammarid's swimming behaviour. We used automated video tracking and analysis to asses a range of swimming behaviours of G. pulex, including swimming speed, startle responses after light transition, acceleration, curvature and thigmotaxis. We found larger effects on the swimming behaviour of G. pulex due to experimental conditions than due to tested antidepressant concentrations. Gammarids swam faster, more straight and showed a stronger startle response during light transition when kept under semi-field conditions compared to the laboratory. Effects found for different test durations were opposite in the laboratory and semi-field. In the laboratory gammarids swam slower and spent more time at the inner zone of the arena after 2 days compared to 21 days while for the semi-field the reverse was observed. Fluoxetine had only minor impacts on the swimming behaviour of G. pulex, but experimental conditions influenced behavioural outcomes in response to fluoxetine exposure. Overall, our results highlight the im-portance of standardizing and optimizing experimental protocols that assess behaviour to achieve reproducible results in ecotoxicology. This project was funded by TKI (Topconsortium voor Kennis en Innovatie) Deltatechnology, jointly financed by the Dutch government and commercial parties through the Precaution project: PREdicting the Chemical sensitivity of AqUaTIc OrgaNisms. This project was also funded by the Netherlands Organization for Scientific Research (NWO) domain TTW through the EMERCHE project: Effect-directed Monitoring tools to assess Ecological and human health Risks of CHemicals of Emerging concern in the water cycle (File number 15760).

Anthropogenic environmental changes, or ‘stressors’, increasingly threaten biodiversity and ecosystem functioning worldwide. Multiple-stressor research is a rapidly expanding field of science that seeks to understand and ultimately predict the interactions between stressors. Reviews and meta-analyses of the primary scientific literature have largely been specific to either freshwater, marine or terrestrial systems, or ecotoxicology. In this cross-disciplinary study, we review the state of knowledge within and among these disciplines to highlight commonality and division in multiple-stressor research. Our review goes beyond a description of previous research by using quantitative bibliometric analysis to identify the division between disciplines and link previously disconnected research communities. Towards a unified research framework, we discuss the shared goal of increased realism through both ecological and temporal complexity, with the overarching aim of improving predictive power. In a rapidly changing world, advancing our understanding of the cumulative ecological impacts of multiple stressors is critical for biodiversity conservation and ecosystem management. Identifying and overcoming the barriers to interdisciplinary knowledge exchange are necessary in rising to this challenge. Division between ecosystem types and disciplines is largely a human creation. Species and stressors cross these borders and so should the scientists who study them.

SOLUTIONS is a European Union Seventh Framework Programme Project (EU-FP7) that aims to deliver a solution-oriented conceptual framework for the evidence-based development of environmental policies with regard to water quality and its protection against contamination. This will integrate innovative chemical and effect-based monitoring tools with a full set of exposure, effect and risk assessment models and strategies to assess abatement options. Uniquely, SOLUTIONS takes advantage of (i) expertise of leading European scientists of major FP6/FP7 projects on chemicals in the water cycle, (ii) access to the infrastructure necessary to investigate the large basins of the Danube and Rhine as well as relevant Mediterranean basins as case studies, and (iii) innovative approaches for stakeholder dialogue and support. In particular, the EU Water Framework Directive (WFD) Common Implementation Strategy (CIS) working groups, International River Commissions, and water works associations will be directly supported with consistent guidance for the early detection, identification, prioritization, and abatement options for chemicals in the water cycle. A set of predictive models and tools will support stakeholders’ management decisions by benefiting from the wealth of data generated from monitoring and chemical registration. SOLUTIONS will provide a specific emphasis on concepts and tools for the impact and risk assessment of complex mixtures of emerging pollutants, their metabolites and transformation products. Analytical and effect-based screening tools will be applied together with ecological assessment tools for the identification of toxicants and their impacts. Beyond state-of-the-art monitoring and management, tools will be elaborated allowing risk identification for aquatic ecosystems and human health. The SOLUTIONS approach will provide transparent and evidence-based suggestions of River Basin Specific Pollutants for the case study basins and support future review of priority pollutants under the WFD as well as potential abatement options., JRC.H.1-Water Resources

We report on thin-film processing improvements in the fabrication of superconducting quasiparticle-trap-assisted electrothermal-feedback transition-edge sensors (QETs) used in the design of Cryogenic Dark Matter Search (CDMS) detectors. The work was performed as part of a detector upgrade project that included optimization of a new confocal sputtering system and development of etch recipes compatible with patterning 40 nm-thick, mixed-phase W films deposited on 300-600 nm-thick, patterned Al. We found that our standard exothermic Al wet etch recipes provided inadequate W/Al interfaces and led to poor device performance. We developed a modified Al wet-etch recipe that effectively mitigates geometrical step-coverage limitations while maintaining our existing device design. Data presented here include SEM and FIB images of films and device interfaces obtained with the new Al etch method. We also introduce a method for quantitatively measuring the energy collection efficiency through these interfaces., Comment: 9 pages; 7 figures

Presently the CDMS-II collaboration's Weakly Interacting Massive Particle (WIMP) search at the Soudan Underground Laboratory sets the most stringent exclusion limits of any WIMP cold dark matter direct-detection experiment. To extend our reach further, to WIMP-nucleon cross sections in the range $10^{-46} - 10^{-44}$cm$^2$, we propose SuperCDMS, which would take advantage of a very deep site. One promising site is the recently approved SNOLab facility in Canada. In this paper we will present our overall plan, identify primary issues, and set the goals that need to be met prior to embarking upon each phase of SuperCDMS., Submitted to Texas Symposium on Relativistic Astrophysics held at Stanford, Dec. 2004. Paper has 6 pages, 4 figures