Search

Proteins of the RNF183 (RING finger 183) family proteins have been reported to be of great importance in tumor the initiation and progression. However, the biological role and regulatory mechanism of RNF183 in non small cell lung cancer (NSCLC) development and progression are poorly defined. Hence, lung adenocarcinoma (LUAD) cell proliferation, cell apoptosis and cell cycle were measured using Cell Counting Kit-8 and flow cytometry analysis, respectively. The correlation between RNF183 and SHP2 (Src homology-2 domain-containing protein tyrosine phosphatase) was measured using coimmunoprecipitation and ubiquitination analysis in vitro. Tumor growth of NSCLC cells in vivo was measured using the nude mouse xenograft model. In this study, we verify that elevated RNF183 expression in tumor tissues of LUAD, origin from the TCGA, GEPIA, TIMER, and UALCAN database. RNF183 regulates apoptosis and cell cycle in vitro and tumor growth in vivo by activating the STAT3 pathway through ubiquitination of SHP2, a negative feedback regulator of the STAT3 pathway. Taken together, our results demonstrate that RNF183 regulates proliferation, apoptosis, and cell cycle in LUAD cells via modulation of SHP2/STAT3 signaling, suggesting the potential for targeting the RNF183-SHP2/STAT3 pathway for use in LUAD treatment.

A graph is said to be symmetric if its automorphism group is transitive on its arcs. This article is one of a series of articles devoted to characterizing prime-valent arc-transitive graphs of square-free order or twice square-free order. In this article, we determine all 7-valent symmetric graphs of order 2pq and 11-valent symmetric graphs of order 4pq.

In this paper, we present a complete list of connected arc-transitive graphs of square-free order with valency 11. The list includes the complete bipartite graph [Formula: see text], the normal Cayley graphs of dihedral groups and the graphs associated with the simple group [Formula: see text] and [Formula: see text], where [Formula: see text] is a prime.

Pleckstrin homology like domain family A member 1(PHLDA1) is also known as T-cell death-associated gene 51 (TDAG51).Studies have demonstrated that the abnormal expression of PHLDA1 is closely associated with the formation,development,and metastasis of tumors.We summarized the latest research advances in the structure and biological properties of PHLDA1,as well as the roles of PHLDA1 in multiple malignanttumors such as breast cancer,cancer,liver gastric cancer,liver cancer,melanoma,and osteosarcoma,aiming to comprehensively reveal the significance of PHLDA1 in the clinical diagnosis of tumors.

Knowledge of the thermal and dielectric properties of wolfberries is essential for understanding the heat transfer and the interaction between the electromagnetic field (10–3000 MHz) and the sample during radio frequency (RF) and microwave (MW) drying. The thermal and dielectric properties of wolfberries were determined as influenced by moisture content from 15.1% to 75.2%, w.b.) and temperature from 25 to 85 °C. The results showed that as the moisture content increased from 15.1% to 75.2% (w.b.), the true density of wolfberries decreased, but the specific heat capacity and thermal conductivity increased with increasing temperature and moisture content. The dielectric properties (DPs) of wolfberries decreased with increasing frequency from 10 to 3000 MHz. The dielectric constant increased with increasing temperature at lower a moisture content (below 45% w.b.) but decreased with increasing temperature at a high moisture content (above 60% w.b.). The cubic and quadratic polynomial models (R2 = 0.977 − 0.997) were best for fitting the dielectric constant and loss factor at four representative frequencies of 27, 40, 915, and 2450 MHz, respectively. The penetration depth increased with the decreased frequency, temperature, and moisture content, and was greater at RF frequencies than MW range, making the RF heating more effective for drying bulk wolfberries. These findings offered essential data before optimizing RF or MW dehydration protocols for wolfberries via computer simulation.

This study aimed to investigate the relationship between centrifugal lipodystrophy (CLD) and lupus erythematosus panniculitis (LEP), and the efficacy and safety of hydroxychloroquine (HCQ) for treating CLD in children. A total of 29 cases clinically diagnosed as CLD (n = 24) and CLD/LEP overlap (n = 5) were enrolled and all were confirmed by skin biopsies of CLD and LEP. The clinicopathological findings, clinical outcomes, and prognosis with the treatment of HCQ between CLD and LEP were compared. All 29 cases (male: female = 1:1.6; median age at onset: 3 years) had cutaneous lesions of centrifugally expanding lipoatrophy, of which five cases overlapped with LEP lesions presented as erythematous indurated plaque (n = 2), subcutaneous nodules (n = 2) and alopecia along Blaschko's lines (n = 1). Antinuclear antibodies were found in six (25.0%) CLD and two (40.0%) overlapped patients (p = 0.597). Histopathologically, of the 24 cases of CLD, 14 (58.5%) exhibited subcutis loss or mild lobular inflammation. Ten (41.7%) cases displayed lobular panniculitis with moderate to dense lymphohistiocytic infiltrate and plasma cells, similar to the five cases of overlap. Small clusters of CD123 positive plasmacytoid dendritic cells were found in 62.5% (5/8) of CLD and 66.7% (2/3) of overlap cases (p 0.99). HCQ (5 mg/kg/d) treatment showed improvement in 91.3% (21/23) of CLD and all overlap cases, including four cases unresponsive to previous oral glucocorticosteroid treatment. Our findings suggested that CLD and LEP represent a spectrum within the same disease. HCQ (5 mg/kg/d) was effective and safe for treating CLD (age 1.5 years), and early treatment and a regular long-term follow-up are essential.

Research has shown that dendrobium officinale polysaccharide (DOP) can promote follicular development and inhibit the apoptosis of ovarian granular cells in PCOS rats. However, DOP cannot be absorbed directly by the stomach and small intestine but is degraded into short-chain fatty acids by gut microbiota in the large intestine and regulates the composition of gut microbiota. How DOP improved ovarian function in PCOS rats through the blood–brain barrier is unclear. In this study, we generated letrozole-induced PCOS rat models and studied the therapeutic effect and mechanism of DOP. 16S rRNA amplicon sequencing analysis, GC-MS short-chain fatty acid detection, and Gene Expression Omnibus database searching were conducted to screen the significantly changed pathways, and a series of experiments, such as enzyme-linked immunosorbent assay, RT-qPCR, Western blot, and immunohistochemistry, were performed. We found that DOP treatment could improve ovarian morphology and endocrine disorders, restore the normal estrus cycle, increase gut microbiota α diversity, and alter β diversity and enrichment of butyrate-producing bacterium in PCOS rats. In addition, compared with PCOS rats, those treated with DOP exhibited higher butyrate and polypeptide YY levels, possibly due to the regulation of G protein-coupled receptor 41 expression. These results indicated that DOP relieved the symptoms of PCOS rats which may be related to the mechanism of butyrate dependent gut–brain–ovary axis protection.

In this study, we investigated the mechanistic role and prognostic significance of IGSF10 in lung adenocarcinoma. Oncomine database analysis showed that IGSF10 expression was significantly reduced in most cancer types, including lung adenocarcinoma (LUAD). In the TCGA-LUAD dataset, IGSF10 expression correlated positively with proportions of tumor-infiltrated B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cells. Kaplan-Meier survival analysis showed that overall survival of patients with low IGSF10 expression was significantly shorter than those with high IGSF10 expression. MiRWalk2.0 database analysis and dual luciferase reporter assays confirmed that miR-106b-5p suppressed IGSF10 expression by binding to its 3’UTR. MiR-106b-5p levels inversely correlated with IGSF10 expression in the TCGA-LUAD dataset. Moreover, inhibition of miR-106b-5p significantly decreased in vitro proliferation, migration, and invasion by LUAD cells, whereas miR-106b-5p overexpression reversed those effects. These results demonstrate that IGSF10 is an independent prognostic factor for LUAD. Furthermore, miR-106b-5p suppressed IGSF10 expression in LUAD tissues by binding to its 3’UTR, which makes IGSF10 and miR-106b-5p potential prognostic biomarkers and therapeutic targets in LUAD patients.

BACKGROUND To explore the value of serum soluble fms-like tyrosine kinase 1 (sFlt-1), CXC chemokine ligand 16 (CXCL16), and lipocalin 2 (LCN-2) in the diagnosis and grading of preeclampsia (PE). METHODS A total of 186 patients with PE diagnosed and treated in our hospital were included. According to the disease severity, the patients were divided into the mild PE group (99 cases) and the severe PE group (87 cases). A total of 72 healthy pregnant women who underwent antenatal care were selected as the healthy control group. The levels of serum sFlt-1, CXCL16, and LCN-2 before medication were compared among the patients, and the diagnosis and grading value of the above 3 indicators were analyzed. RESULTS For PE patients vs. healthy controls, the levels of sFlt-1 (132.71±14.49 vs. 68.43±9.28 µg/L), CXCL16 (2.15±0.35 vs. 0.61±0.12 µg/L), and LCN-2 (70.81±8.25 vs. 19.22±3.14 µg/L) were all significantly higher in PE patients than in the healthy controls (P

Understanding bacterial adsorption and the evolution of biofilms on arsenopyrite with different surface structures is of great significance to clarifying the mechanism of microbe-mineral interfacial interactions and the production of acidic mine drainage impacting the environment. In this study, the attachment of Sulfobacillus thermosulfidooxidans cells and subsequent biofilm formation on arsenopyrite with different surface structures in the presence of dissolved As(III) was studied. Arsenopyrite slices with a specific surface were obtained by electrochemical corrosion at 0.26 V. The scanning electronic microscopy-energy dispersion spectra analyses indicated that the arsenopyrite surface deficient in sulfur and iron obtained by electrochemical treatment was not favorable for the initial adsorption of bacteria, and the addition of As(III) inhibited the adsorption of microbial cells. Epifluorescence microscopy showed that the number of cells attaching to the arsenopyrite surface increased with time; however, biofilm formation was delayed significantly when As(III) was added.

A Cayley graph [Formula: see text] is said to be normal if [Formula: see text] is normal in [Formula: see text]. In this paper, we investigate the normality problem of the connected 11-valent symmetric Cayley graphs [Formula: see text] of finite nonabelian simple groups [Formula: see text], where the vertex stabilizer [Formula: see text] is soluble for [Formula: see text] and [Formula: see text]. We prove that either [Formula: see text] is normal or [Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text] or [Formula: see text]. Further, 11-valent symmetric nonnormal Cayley graphs of [Formula: see text], [Formula: see text] and [Formula: see text] are constructed. This provides some more examples of nonnormal 11-valent symmetric Cayley graphs of finite nonabelian simple groups after the first graph of this kind (of valency 11) was constructed by Fang, Ma and Wang in 2011.

Research has shown that dendrobium officinale polysaccharide (DOP) can promote follicular development and inhibit the apoptosis of ovarian granular cells in PCOS rats. However, DOP cannot be absorbed directly by the stomach and small intestine but is degraded into short-chain fatty acids by gut microbiota in the large intestine and regulates the composition of gut microbiota. How DOP improved ovarian function in PCOS rats through the blood-brain barrier is unclear. In this study, we generated letrozole-induced PCOS rat models and studied the therapeutic effect and mechanism of DOP. 16S rRNA amplicon sequencing analysis, GC-MS short-chain fatty acid detection, and Gene Expression Omnibus database searching were conducted to screen the significantly changed pathways, and a series of experiments, such as enzyme-linked immunosorbent assay, RT-qPCR, Western blot, and immunohistochemistry, were performed. We found that DOP treatment could improve ovarian morphology and endocrine disorders, restore the normal estrus cycle, increase gut microbiota α diversity, and alter β diversity and enrichment of butyrate-producing bacterium in PCOS rats. In addition, compared with PCOS rats, those treated with DOP exhibited higher butyrate and polypeptide YY levels, possibly due to the regulation of G protein-coupled receptor 41 expression. These results indicated that DOP relieved the symptoms of PCOS rats which may be related to the mechanism of butyrate dependent gut-brain-ovary axis protection.

In the last decades, different non-thermal and thermal technologies have been developed for food processing. However, in many cases, it is not clear which experimental parameters must be reported to guarantee the experiments' reproducibility and provide the food industry a straightforward way to scale-up these technologies. Since reproducibility is one of the most important science features, the current work aims to improve the reproducibility of studies on emerging technologies for food processing by providing guidelines on reporting treatment conditions of thermal and non-thermal technologies. Infrared heating, microwave heating, ohmic heating and radiofrequency heating are addressed as advanced thermal technologies and isostatic high pressure, ultra-high-pressure homogenization sterilization, high-pressure homogenization, microfluidization, irradiation, plasma technologies, power ultrasound, pressure change technology, pulsed electric fields, pulsed light and supercritical CO2 are approached as non-thermal technologies. Finally, growing points and perspectives are highlighted.

Objective: This paper explores the application of online education mode for a postgraduate teaching education and its influence on postgraduate development under the background of COVID-19. Methods: This paper discusses the innovation modes of various online education platforms in postgraduate teaching, and analyzes the influence of online education in postgraduate curriculum education and academic ability education. Results: After the outbreak of COVID-19, online teaching methods are more abundant and innovative content is more, which stimulated the learning enthusiasm of postgraduates. Online platforms provide more opportunities for academic exchanges among postgraduates, which helps to improve the teaching quality of postgraduates. Conclusion: Online education has gradually developed into a more effective and potential education method. Combined with the application status of online teaching mode in postgraduate education, this paper provides specific development suggestions for universities and colleges to further construct and develop high-quality online teaching mode for postgraduates.

The postgraduate educational reform of medical colleges and universities in minority areas focuses on the improvement of medical postgraduates’ cultivation quality. This study analyses the deficiencies in the innovative ability cultivation of medical postgraduates in minority areas from the four aspects of theoretical knowledge, clinical skills, experimental skills and the scientific research thinking and abilities, which is on the basis of postgraduate innovative ability. As a mainstream development mode of postgraduate education, IUR (Industry-University-Research) will be an effective method to improve the innovative ability of medical postgraduates with its own advantage and a combination of the characteristics of medical postgraduate education in minority areas.

Curriculum ideological and political education is the core idea and direction of the construction of online teaching teams in medical colleges and universities. With the development of information and intelligence, online teaching has become one of the important modes of education and teaching development in medical colleges and universities. The construction of online teaching teams plays a key role in the development of online teaching, with four core problems existing at present, including unclear teaching team goals, loose teaching team organization, imperfect teaching team systems and difficulties in achievement transformation. The solutions of the four core problems can be found by following the guidance of curriculum ideological and political education and the regularity of the teaching team development. Therefore, in this study, with the construction of online teaching teams in medical colleges and universities as an example, the role and value of curriculum ideological and political education in the construction of teaching team are analyzed deeply to provide the feasible direction of teaching reform in medical colleges and universities.

Purpose: To build a “Four-in-one” medical talent training mode driven by “Wisdom, Integration and Characteristics” in ethnic minority areas to improve students’ humanistic accomplishment, practical and innovative ability and post competence and train high-quality characteristic application-oriented medical talents. Solutions: The “Four-in-one” medical talent training mode driven by “Wisdom, Integration and Characteristics” is created and applied to the medical talent training practice of our school by taking the educational informationization as the endogenous variable of systematic reform of education, and “integration of science and education, integration of production and education, integration of theory and practice” as the focus to improve the quality of medical talent training, focusing on the four elements of “knowledge imparting, ability development, career skill development and value shaping” in talent training, combining the functions of “scientific research, social service, cultural inheritance and innovation” of colleges and universities, and launching the reform from the aspects of “thick foundation, strong ability, pro-industry, and humanities (Four-in-one)”. Results: The students’ humanistic accomplishment, practical ability, innovation and entrepreneurship ability and post competence have been significantly improved; the level and gradation of running school have been greatly enhanced. Conclusion: The construction of “Four-in-one” medical talent training mode driven by “Wisdom, Integration and Characteristics” in ethnic minority areas can help improve the training quality of medical talents.

Objective: Explore the new system for constructing a supervisor team of masters of medicine under the mode of double track integration. Methods: Based on the current construction of supervisor team for masters of medicine under the mode of double track integration, this paper tries to find proper methods to improve the construction of supervisor team for masters of medicine proceed from the discipline development, academic level, clinical and scientific research system, supervisor teaching arrangement, and transformation ability of science and technology. Results: Under the mode of double track integration, five aspects of the supervisor training system, selection system, supervisor group system, course leader system, and industry-study-research mode need to be integrated efficiently, thereby providing strong support for the development of masters of medicine supervisor team. Conclusion: In view of the current construction of the supervisor team for masters of medicine under the mode of double track integration, further integration will provide a feasible direction for the construction of the supervisor team for masters of medicine.

Purpose: To build a medical talent training mode based on “integration of production and education, integration of science and education and integration of theory and practice”. Solutions: The talent training mode based on “integration of production and education, integration of science and education and integration of theory and practice” is finally built by taking “integration of science and education, integration of production and education, integration of theory and practice” as the focus of improving the quality of talent training to strengthen deep cooperation between universities and industries of talent training and deepen school-enterprise cooperation, taking students’ participation in scientific research as an effective form of education to strengthen the construction of practical teaching bases, and using the project-based teaching methods such as PAD Class and flipped classroom to promote the close combination of theoretical and practical teaching. Results: This model underlines students and cultivates their ability to find and solve problems in practice, and enhances their post competence and comprehensive professional ability. Conclusion: The education mode in medical talent training of “integration of production and education, integration of science and education, and integration of theory and practice” is an effective way to train high-quality application-oriented undergraduate talents to improve students’ career planning ability and employment competitiveness.

Objective: Explore new methods and functions of ideological and political education in the construction of online courses. Methods: Taking the construction of the online course of Chinese medicine pharmacology as an example, the necessity of ideological and political education in the construction of online course is analyzed. At the same time, combined with the disciplinary characteristics of traditional Chinese medicine pharmacology, it analyzes the new methods and effects of ideological and political education integration. Results: Ideological and political education integrates the construction of online courses, which can be integrated into the foreword of the times, models of the times, cutting-edge technology and professional cases, so as to enhance students’ patriotism, ideological morality, innovative thinking and professional skills. Conclusion: Ideological and political education runs through the construction of online courses, so as to provide new ideas for the training of students majoring in Chinese pharmacy.

Objective: To explore new methods and functions of the new model of ideological and political education integrating production, teaching and research in the cultivation of medical graduates’ innovative ability in ethnic areas. Methods: Starting from the status quo of the innovation ability of medical postgraduates in ethnic areas, it deeply analyzes the requirements of the innovation ability of medical postgraduates in ethnic areas under the background of the new era. Based on the actual situation of medical schools in ethnic regions, combined with the new model of ideological and political education integrating production, teaching and research, it analyzes its new methods and functions in promoting graduate students’ innovative capabilities. Results: The new model of ideological and political education integrating production, education and research can realize the improvement of students’ internal driving force, the rationality of the teaching staff, the optimization of platform resources, the standardization of system construction, and the high-level and applicability of scientific research results. Conclusion: The new model of ideological and political education integrating production, teaching and research is a powerful boost for the standardized training and innovation of medical graduate students in ethnic areas.

As a potential oncogene, nucleolar and spindle‑associated protein 1 (NUSAP1) is involved in the regulation of tumor cell proliferation, metastasis and drug resistance. However, the role of NUSAP1 in non‑small cell lung cancer (NSCLC) remains unclear. The present study aimed to investigate the biological function and underlying molecular mechanisms of NUSAP1 in NSCLC. NUSAP1 expression was measured in NSCLC tissues and cell lines via immunohistochemistry and western blotting, respectively. NSCLC cell lines stably inhibiting NUSAP1 were established to investigate its effects on cell proliferation, colony formation and invasion, and on in vivo tumorigenicity. Additionally, the upstream and downstream mechanisms of NUSAP1 in regulating NSCLC progression were investigated. The results indicated that NUSAP1 expression was upregulated in NSCLC tissues and cell lines. High NUSAP1 expression was associated with tumor size, TNM stage, lymph node metastasis and poor patient survival, whereas knockdown of NUSAP1 inhibited NSCLC cell proliferation, colony formation and invasion. Furthermore, downregulation of NUSAP1 decreased the growth of NSCLC xenografts in vivo. In addition, myocyte enhancer factor 2D (MEF2D) directly targeted the NUSAP1 promoter, thereby enhancing the mRNA and protein expression levels of NUSAP1. Moreover, the results demonstrated that MEF2D expression was upregulated in NSCLC tissues and was positively correlated with NUSAP1 expression. MEF2D‑knockdown decreased NSCLC cell proliferation, colony formation and invasion. NUSAP1 upregulation reversed the effects of MEF2D‑knockdown on NSCLC progression. Furthermore, it was observed that MEF2D‑knockdown inhibited the accumulation and nuclear translocation of β‑catenin, thereby repressing the activation of the Wnt/β‑catenin signaling pathway in NSCLC cells, whereas NUSAP1 upregulation rescued the effects of MEF2D‑knockdown on the activation of the Wnt/β‑catenin signaling pathway. In conclusion, the findings of the present study indicated that the MEF2D/NUSAP1 signaling pathway promoted NSCLC progression by inducing the activation of Wnt/β‑catenin signaling, and this novel mechanism may represent a potential treatment target for patients with NSCLC.

A highly stereoselective umpolung 1,4-addition of vinyl bromides to enals is enabled by NHC/palladium cooperative catalysis, generating various valuable γ,δ-unsaturated carbonyl derivatives in excellent yields (up to 90%). A detailed mechanism investigation indicates the NHC act as both organocatalyst and ligand for palladium during this system.

Here, NaGdF

The rapid spread of the coronavirus disease (COVID-19) pandemic in over 200 countries poses a substantial threat to human health. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes COVID-19, can be discharged with feces into the drainage system. However, a comprehensive understanding of the occurrence, presence, and potential transmission of SARS-CoV-2 in sewers, especially in community sewers, is still lacking. This study investigated the virus occurrence by viral nucleic acid testing in vent stacks, septic tanks, and the main sewer outlets of community where confirmed patients had lived during the outbreak of the epidemic in Wuhan, China. The results indicated that the risk of long-term emission of SARS-CoV-2 to the environment via vent stacks of buildings was low after confirmed patients were hospitalized. SARS-CoV-2 were mainly detected in the liquid phase, as opposed to being detected in aerosols, and its RNA in the sewage of septic tanks could be detected for only four days after confirmed patients were hospitalized. The surveillance of SARS-CoV-2 in sewage could be a sensitive indicator for the possible presence of asymptomatic patients in the community, though the viral concentration could be diluted more than 10 times, depending on the sampling site, as indicated by the

Cancer, the most significant cause of morbidity and mortality, has already posed a heavy burden on health care systems globally. In recent years, cancer treatment has made a significant breakthrough, but cancer cells inevitably acquire resistance, and the efficacy of the treatment is greatly reduced as the tumor progresses. To overcome the above issues, novel chemotherapeutics are needed urgently. Artemisinin and its derivatives-sesquiterpene lactone compounds possessing a unique peroxy bridge moiety-exhibit excellent safety and tolerability profiles. Mechanistically, artemisinin derivatives can promote cancer cell apoptosis, induce cell cycle arrest and autophagy, and inhibit cancer cell invasion and migration. Accordingly, artemisinin derivatives demonstrate promising anticancer efficacy both in vitro and in vivo, and even in clinical Phase I/II trials. The purpose of the present review article is to provide an emphasis on the current scenario (January 2017-January 2022) of artemisinin derivatives with potential anticancer activity, inclusive of artemisinin metal complexes, hybrids, and dimers. The structure-activity relationships and mechanisms of action are also discussed to facilitate the further rational design of more effective candidates.

Under investigation in this paper is a coupled generalized nonlinear Schrodinger–Boussinesq system, which describes the coupled upper-hybrid and magnetoacoustic modes in a homogeneous magnetized plasma for the bidirectional propagation near the magnetoacoustic speed. Based on the Hirota method, the expressions for the multi-soliton solutions are given. Effects of the group velocity, group dispersion coefficient for the upper-hybrid, and the properties of the magnetic field on the soliton are discussed. Based on the asymptotic analysis, interaction between two solitons is proved to be elastic through the asymptotic analysis. Position at which the maximal distortion occurs is obtained. Multi-soliton interaction is illustrated and investigated. Two prerequisites of the formation and features of the bound state are discussed. For the cases of three solitons, inelastic interaction occurs with phase shifts. Characteristics of the breather and its relation with the bound state and the breather are investigated. Interaction between the bound state (even the breather) and a single soliton is discussed for both cases that they are parallel or not.

Proteins of the RNF183 (RING finger 183) family proteins have been reported to be of great importance in tumor the initiation and progression. However, the biological role and regulatory mechanism of RNF183 in non small cell lung cancer (NSCLC) development and progression are poorly defined. Hence, lung adenocarcinoma (LUAD) cell proliferation, cell apoptosis and cell cycle were measured using Cell Counting Kit-8 and flow cytometry analysis, respectively. The correlation between RNF183 and SHP2 (Src homology-2 domain-containing protein tyrosine phosphatase) was measured using coimmunoprecipitation and ubiquitination analysis in vitro. Tumor growth of NSCLC cells in vivo was measured using the nude mouse xenograft model. In this study, we verify that elevated RNF183 expression in tumor tissues of LUAD, origin from the TCGA, GEPIA, TIMER, and UALCAN database. RNF183 regulates apoptosis and cell cycle in vitro and tumor growth in vivo by activating the STAT3 pathway through ubiquitination of SHP2, a negative feedback regulator of the STAT3 pathway. Taken together, our results demonstrate that RNF183 regulates proliferation, apoptosis, and cell cycle in LUAD cells via modulation of SHP2/STAT3 signaling, suggesting the potential for targeting the RNF183-SHP2/STAT3 pathway for use in LUAD treatment.

An umpolung 1,4-addition of aryl iodides to enals promoted by cooperative (terpy)Pd/NHC catalysis was developed that generates various bioactive β,β-diaryl propanoate derivatives. This system is not only the first reported palladium-catalyzed arylation of NHC-bound homoenolates but also expands the scope of NHC-induced umpolung transformations. A diverse array of functional groups such as esters, nitriles, alcohols, and heterocycles are tolerated under the mild conditions. This method also circumvents the use of moisture-sensitive organometallic reagents.

Archery is a fine-motor-skill sport, in which success results from multiple factors including a fine neuromuscular tuning. The present study hypothesised that lower trapezius specific training can improve archers’ performance with concomitant changes in muscle activity and shoulder kinematics. We conducted a prospective study in a university archery team. Athletes were classified into exercise and control groups. A supervised lower trapezius muscle training program was performed for 12 weeks in the exercise group. The exercise program focused on a lower trapezius-centred muscular training. Performance in a simulated game was recorded as the primary outcome, and shoulder muscle strength, kinematics, and surface electromyography were measured and analysed. In the exercise group, the average score of the simulation game increased from 628 to 639 after the training regimens (maximum score was 720), while there were no such increases in the control group. The lower trapezius muscle strength increased from 8 to 9 kgf after training regimens and shoulder horizontal abductor also increased from 81 to 93 body weight% for the exercise group. The upper/lower trapezius ratio decreased from 2.2 to 1.1 after training. The lower trapezius exercise training regimen could effectively improve the performance of an archer with a simultaneous increase in shoulder horizontal abductor and lower trapezius muscle strength.

Gram-negative Aeromonas bacteria is known to contaminate aquaculture products and retains the ability to infect wide range of host including fishes, shrimps and humans. This study is designed to evaluate the presence of Aeromonas in healthy loaches (Misgurnus anguillicaudatus) collected from the markets in Hainan and Guangdong provinces of China. Based on the molecular sequencing and phylogenetic analysis with single 16S rRNA and concatenated genes (gyrA and ropD), 104 isolates were identified as Aeromonas species followed by further classification to A. veronii (83.65%), A. jandaei (9.62%), A. hydrophila (3.85%) and A. allosaccharophila (2.88%). More than half of the isolates displayed hemolytic activity of 87.5% and were able to form moderate biofilm (78.26%). Fourteen antibiotics from ten representative classes were screened that demonstrated complete resistance to ampicillin, 89.4% and 68.3% to lincomycin and nalidixic acid. Moreover, a notable detection and prevalence was found in screening of ten virulence-related and nine resistance genes. To our knowledge, it is the first report of its kind demonstrating potential threat of the commercial loaches carrying Aeromonas to human health. These findings will assist professionals working in clinical settings to optimize their prescription accordingly and researchers to access the impacts of Aeromonas resistance on human health.ImportanceThis study demonstrates high infection rate of loaches by Aeromonas spp. in Hainan and Guangdong provinces of China.The most prevalent species was Aeromonas veronii.Most of the isolates displayed hemolytic activity and formed moderate biofilm.Multiple virulence genes, resistance genes and multi-antibiotic resistance were detected.Aeromonads risks should be considered in loach industry.

In this paper, we focus on the interaction behavior associated with a generalized (2+1)-dimensional Hirota bilinear equation. With symbolic computation, two types of interaction solutions including lump-kink and lump-soliton ones are derived through mixing two positive quadratic functions with an exponential function, or two positive quadratic functions with a hyperbolic cosine function in the bilinear equation. The completely non-elastic interaction between a lump and a stripe is presented, which shows the lump is drowned or shallowed by the stripe. The interaction between lump and soliton is also given, where the lump moves from one branch to the other branch of the soliton. These phenomena exhibit the dynamics of nonlinear waves and the solutions are useful for the study on interaction behavior of nonlinear waves in shallow water, plasma, nonlinear optics and Bose–Einstein condensates.

In this paper, a general propagation lattice Boltzmann model for a variable-coefficient compound Korteweg-de Vries-Burgers (vc-cKdVB) equation is investigated through selecting equilibrium distribution function and adding a compensation function, which can provide some more realistic models than their constant-coefficient counterparts in fluids or plasmas. Chapman–Enskog analysis shows that the vc-gKdVB equation can be recovered correctly from the present model. Numerical simulations in different situations of this equation are conducted, including the propagation and interaction of the bell-type, kink-type and periodic-depression solitons and the evolution of the shock-wave solutions. It is found that the numerical results match well with the analytical solutions, which demonstrates that the current lattice Boltzmann model is a satisfactory and efficient algorithm. In addition, it is also shown the present model could be more stable and more accurate than the standard lattice Bhatnagar–Gross–Krook model through adjusting the two free parameters introduced into the propagation step.

Food grains constitute a vital part of the daily diet of the population worldwide, and are generally considered as safe products with high storage stability due to their low moisture contents. However, post-harvest losses (PHL) caused by insects, fungi, food-borne pathogens, and undesirable enzymes remain a major concern for the grain industry. Thermal treatments are commonly used to reduce the PHL of grains and their products without any chemical residues. Among which, radio frequency (RF) technology has been regarded as a promising alternative to traditional heating methods for improving safety and quality of food grains due to its fast, volumetric, and deep penetration heating characteristics. This review provided comprehensive information about principles of RF technology and its main applications including disinfestation, pasteurization, enzyme inactivation, drying, and roasting for processing food grains and their products. The methods to improve the RF heating uniformity and effects of RF heating on product quality were also reviewed. Finally, the current problems and recommendations for future work related to RF processing of grains and their products were discussed. This review would improve the understanding of RF heating for food grains and their products and promote the application of RF technology in the food grain industry.