1. Structure of the human adult hemoglobin minor fraction A1b by electrospray and secondary ion mass spectrometry. Pyruvic acid as amino-terminal blocking group
- Author
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Danielle Promé, Jean-Claude Promé, C. Ponthus, Blouquit Y, and J Rosa
- Subjects
Glycated Hemoglobin ,chemistry.chemical_classification ,Electrospray ,Hemeprotein ,Chromatography ,Macromolecular Substances ,Electrospray ionization ,Molecular Sequence Data ,Peptide ,Cell Biology ,Mass spectrometry ,Biochemistry ,Mass Spectrometry ,Peptide Fragments ,Amino acid ,chemistry.chemical_compound ,chemistry ,Pyruvic Acid ,Humans ,Trypsin ,Amino Acid Sequence ,Pyruvic acid ,Hemoglobin ,Pyruvates ,Molecular Biology - Abstract
Hemoglobin A1b is a minor hemoglobin component from human hemolysate (less than 0.5% of total hemoglobin) whose structure has never been established. It was purified and studied by mass spectrometry. Electrospray ionization of its abnormal beta-chain indicated a 70-Da mass increase. Separation of the trytic digest by reversed-phase liquid chromatography revealed an abnormal beta T1 peptide. Cesium ion bombardment ionization produced a protonated molecular ion at m/z 1022.516, showing an additional C3H2O2 residue to normal beta T1. The amino acid sequences of both abnormal and normal beta T1 peptides were found identical by comparison of their collision activation spectra. Time course hydrolysis of abnormal beta T1 indicated a rapid loss of the modifying group, leading to normal beta T1. At least, mild treatment with acidic methanol showed an additional methylated site, comparatively with normal beta T1. All these results are consistent with a ketimine-linked pyruvic acid at the amino end of the beta-chain of hemoglobin.
- Published
- 1991