1. A Multichannel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments
- Author
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Bartic, C., Battaglia, F.P., Wang, L., Nguyen, Thoa T., Cabral, H., Navratilova, Z., Kianianmomeni, A., and Kianianmomeni, A.
- Subjects
Neuroinformatics ,0301 basic medicine ,Opsin ,biology ,Computer science ,Optogenetics ,Recording system ,03 medical and health sciences ,Microelectrode ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Spike sorting ,Neuronal circuits ,Rhodopsin ,Methods in Molecular Biology ,Neuromodulation ,Optical stimulation ,medicine ,biology.protein ,Closed loop ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Selective perturbation of the activity of specific cell types in the brain tissue is essential in understanding the function of neuronal circuits involved in cognition and behavior and might also provide therapeutic neuromodulation strategies. Such selective neuronal addressing can be achieved through the optical activation of light-sensitive proteins called opsins that are expressed in specific cell populations through genetic methods-hence the name "optogenetics." In optogenetic experiments, the electrical activity of the targeted cell populations is optically triggered and monitored using arrays of microelectrodes. In closed-loop studies, the optical stimulation parameters are adjusted based on the recorded activity, ideally in real time. Here we describe the basic tools and the protocols allowing closed-loop optogenic experiments in vivo.
- Published
- 2016