8 results on '"Avolio, R."'
Search Results
2. Spectroscopic study of nanocomposites based on PANI and carbon nanostructures for pH sensors
- Author
-
Grozdanov, A., Petrovski, A., Avella, M., Paunović, P., Errico, M. E., Avolio, R., Gennaro Gentile, Falco, F., and Dimitrov, A. T.
- Subjects
PANI ,nanocomposites ,sensors - Abstract
Nanocomposites of polyaniline (PANI) and two carbon nanostructures - multiwall carbon nanotubes (MWCNTs) and graphene G) were obtained by electrochemical polymerization. PANI based nanocomposites with different concentration of carbon nanostructures (CNS:1, 2 and 3wt%) as well as with different methods for CNS dispersion in the electrolyte, were synthesized. The interactions among the CNS nanostructures and polyaniline matrix were studied and the results confirmed strong interactions among the quinoidal structure of PANI and both CNS. In order to design nanocomposite sensors, PANI/CNS nanocomposites were directly electro-polymerized on gold wires of screen printed electrodes. Their sensing activity was evaluated through the resistivity changes at different pH.
3. Polystyrene nanoplastics affect the human ubiquitin structure and ubiquitination in cells: a high-resolution study
- Author
-
M. della Valle, G. D'Abrosca, M. T. Gentile, L. Russo, C. Isernia, S. Di Gaetano, R. Avolio, R. Castaldo, M. Cocca, G. Gentile, G. Malgieri, M. E. Errico, R. Fattorusso, della Valle, M, D'Abrosca, G, Gentile, Mt, Russo, L, Isernia, C, Di Gaetano, S, Avolio, R, Castaldo, R, Gentile, G, Cocca, M, Malgieri, G, Errico, Me, and Fattorusso, R
- Subjects
General Chemistry - Abstract
Humans are estimated to consume several grams per week of nanoplastics (NPs) through exposure to a variety of contamination sources. Nonetheless, the effects of these polymeric particles on living systems are still mostly unknown. Here, by means of CD, NMR and TEM analyses, we describe at an atomic resolution the interaction of ubiquitin with polystyrene NPs (PS-NPs), showing how a hard protein corona is formed. Moreover, we report that in human HeLa cells exposure to PS-NPs leads to a sensible reduction of ubiquitination. Our study overall indicates that PS-NPs cause significant structural effects on ubiquitin, thereby influencing one of the key metabolic processes at the base of cell viability.
- Published
- 2022
4. Regulation of mitochondrial complex III activity and assembly by TRAP1 in cancer cells
- Author
-
Danilo Swann Matassa, Daniela Criscuolo, Rosario Avolio, Ilenia Agliarulo, Daniela Sarnataro, Consiglia Pacelli, Rosella Scrima, Alessandra Colamatteo, Giuseppe Matarese, Nazzareno Capitanio, Matteo Landriscina, Franca Esposito, Matassa, D. S., Criscuolo, D., Avolio, R., Agliarulo, I., Sarnataro, D., Pacelli, C., Scrima, R., Colamatteo, A., Matarese, G., Capitanio, N., Landriscina, M., and Esposito, F.
- Subjects
Cancer Research ,Oncology ,Ovarian cancer ,Respiratory complex III ,Genetics ,Platinum resistance ,TRAP1 - Abstract
Background Metabolic reprogramming is an important issue in tumor biology. A recently-identified actor in this regard is the molecular chaperone TRAP1, that is considered an oncogene in several cancers for its high expression but an oncosuppressor in others with predominant oxidative metabolism. TRAP1 is mainly localized in mitochondria, where it interacts with respiratory complexes, although alternative localizations have been described, particularly on the endoplasmic reticulum, where it interacts with the translational machinery with relevant roles in protein synthesis regulation. Results Herein we show that, inside mitochondria, TRAP1 binds the complex III core component UQCRC2 and regulates complex III activity. This decreases respiration rate during basal conditions but allows sustained oxidative phosphorylation when glucose is limiting, a condition in which the direct TRAP1-UQCRC2 binding is disrupted, but not TRAP1-complex III binding. Interestingly, several complex III components and assembly factors show an inverse correlation with survival and response to platinum-based therapy in high grade serous ovarian cancers, where TRAP1 inversely correlates with stage and grade and directly correlates with survival. Accordingly, drug-resistant ovarian cancer cells show high levels of complex III components and high sensitivity to complex III inhibitory drug antimycin A. Conclusions These results shed new light on the molecular mechanisms involved in TRAP1-dependent regulation of cancer cell metabolism and point out a potential novel target for metabolic therapy in ovarian cancer.
- Published
- 2022
5. Washing load influences the microplastic release from polyester fabrics by affecting wettability and mechanical stress
- Author
-
Maria Emanuela Errico, Gennaro Gentile, Rachele Castaldo, Michela Volgare, Veronica Ambrogi, Roberto Avolio, Mariacristina Cocca, Francesca De Falco, Volgare, M., De Falco, F., Avolio, R., Castaldo, R., Errico, M. E., Gentile, G., Ambrogi, V., and Cocca, M.
- Subjects
Multidisciplinary ,business.product_category ,Laundry ,Chemistry ,Science ,Microplastics ,release ,Article ,Materials science ,textiles ,Environmental sciences ,Polyester ,Microfiber ,Medicine ,Wetting ,Composite material ,business - Abstract
Microplastics released from textiles during the washing process represent the most prevalent type of microparticles found in different environmental compartments and ecosystems around the world. Release of microfibres during the washing process of synthetic textiles is due to the mechanical and chemical stresses that clothes undergo in washing machines. Several washing process parameters, conditions, formulations of laundering additives have been correlated to microfibre release and some of them have been identified to affect microfibre release during washing process, while no correlation has been evaluated between microfibre release and washing load. In the present study, microfibre release was evaluated as function of the washing load in a real washing process, indicating a progressive decrease of microfibre release with increasing washing load. The quantity of released microfibres increased by around 5 times by decreasing the washing load due to a synergistic effect between water-volume to fabric ratio and mechanical stress during washing. Moreover, the higher mechanical stress to which the fabric is subjected in the case of a low washing load, hinders the discrimination of the effect on the release of other washing parameters like the type of detergent and laundry additives used.
- Published
- 2021
6. Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression
- Author
-
Silvia Piscitelli, Tommaso Russo, Fabiana Passaro, Silvia Parisi, Daniela Castaldo, Rosario Avolio, Giuseppina Divisato, Chiara D'Ambrosio, Paolo Gianfico, Mariorosario Masullo, Alessia Castellucci, Andrea Scaloni, Parisi, S., Castaldo, D., Piscitelli, S., D'Ambrosio, C., Divisato, G., Passaro, F., Avolio, R., Castellucci, A., Gianfico, P., Masullo, M., Scaloni, A., and Russo, T.
- Subjects
Lin28 ,Proteomics ,Cell biology ,Molecular biology ,Science ,Blotting, Western ,RNA-binding protein ,Stem cells ,LIN28 ,Biochemistry ,Article ,DNA Methyltransferase 3A ,Lin28a ,Downregulation and upregulation ,Gene expression ,Humans ,Immunoprecipitation ,DNA (Cytosine-5-)-Methyltransferases ,3' Untranslated Regions ,Messenger RNA ,Multidisciplinary ,biology ,RNA‐binding proteins ,Dnmt3a expression ,embryogenesis ,tumorigenesis ,Helicase ,RNA-Binding Proteins ,Translation (biology) ,Embryonic stem cell ,biology.protein ,Chromatography, Gel ,Medicine ,Lin28, RNA binding protein, translation, pluripotent stem cells, epiblast stem cells, differentiation - Abstract
Lin28 is an evolutionary conserved RNA-binding protein that plays important roles during embryonic development and tumorigenesis. It regulates gene expression through two different post-transcriptional mechanisms. The first one is based on the regulation of miRNA biogenesis, in particular that of the let-7 family, whose expression is suppressed by Lin28. Thus, loss of Lin28 leads to the upregulation of mRNAs that are targets of let-7 species. The second mechanism is based on the direct interaction of Lin28 with a large number of mRNAs, which results in the regulation of their translation. This second mechanism remains poorly understood. To address this issue, we purified high molecular weight complexes containing Lin28a in mouse embryonic stem cells (ESCs). Numerous proteins, co-purified with Lin28a, were identified by proteomic procedures and tested for their possible role in Lin28a-dependent regulation of the mRNA encoding DNA methyltransferase 3a (Dnmt3a). The results show that Lin28a activity is dependent on many proteins, including three helicases and four RNA-binding proteins. The suppression of four of these proteins, namely Ddx3x, Hnrnph1, Hnrnpu or Syncrip, interferes with the binding of Lin28a to the Dnmt3a mRNA, thus suggesting that they are part of an oligomeric ribonucleoprotein complex that is necessary for Lin28a activity.
- Published
- 2020
7. Effect of cellulose structure and morphology on the properties of poly(butylene succinate-co-butylene adipate) biocomposites
- Author
-
Maurizio Avella, Maria Emanuela Errico, Roberto Avolio, Veronica Ambrogi, Mariacristina Cocca, V. Graziano, Y. D. F. Pereira, Gennaro Gentile, Avolio, R, Graziano, V., Pereira, Y. D. F., Cocca, M., Gentile, G., Errico, M. E., Ambrogi, Veronica, and Avella, M.
- Subjects
Thermogravimetric analysis ,Succinate ,Materials science ,Epoxy Compound ,Polymers and Plastics ,Adipates ,Methacrylate ,Permeability ,chemistry.chemical_compound ,Crystallinity ,Tensile Strength ,Adipate ,Materials Chemistry ,Cellulose ,Composite material ,Maleic Anhydride ,Filler ,Maleic Anhydrides ,Materials Chemistry2506 Metals and Alloy ,chemistry.chemical_classification ,Biocomposites ,Polymers and Plastic ,Medicine (all) ,Organic Chemistry ,Structure ,Water ,Chemical modification ,Maleic anhydride ,Succinates ,Polymer ,Polybutylene succinate ,chemistry ,Epoxy Compounds ,Methacrylates ,Volatilization ,Biocomposite ,PBSA - Abstract
Composites based on poly(butylene succinate-co-butylene adipate) (PBSA) containing amorphized and crystalline cellulose reinforcements have been prepared and characterized. In order to improve the polymer/filler interfacial adhesion, an efficient compatibilizing agent has been synthesized by chemical modification of PBSA and characterized by FT-IR, FT-NIR and 1H NMR spectroscopy. Uncompatibilized and compatibilized composites have been tested through morphological, mechanical, calorimetric and thermogravimetric analysis. Moreover, water vapor permeability and biodegradation kinetics of composites have been investigated. The addition to PBSA of cellulose fillers differing from each other by crystallinity degree and morphology, and the use of a compatibilizing agent have allowed modulating tensile and thermal properties, water vapor transmission rate and biodegradation kinetic of the composites.
- Published
- 2015
8. Oxidative metabolism drives inflammation-induced platinum resistance in human ovarian cancer
- Author
-
Matassa DS1, Amoroso MR1, Lu H2, Avolio R1, Arzeni D1, Procaccini C3, Faicchia D4, Maddalena F5, Simeon V5, Agliarulo I1, Zanini E2, Mazzoccoli C5, Recchi C2, Stronach E6, Marone G4, Gabra H6, Matarese G1, Landriscina M5, 7, Esposito F1., Matassa, D S, Amoroso, M R, Lu, H, Avolio, R, Arzeni, D, Procaccini, C, Faicchia, D, Maddalena, F, Simeon, V, Agliarulo, I, Zanini, E, Mazzoccoli, C, Recchi, C, Stronach, E, Marone, G, Gabra, H, Matarese, G, Landriscina, M, Esposito, F, Matassa, DANILO SWANN, Amoroso, MARIA ROSARIA, Avolio, Rosario, Procaccini, Claudio, Faicchia, Deriggio, Agliarulo, Ilenia, Marone, Gianni, Matarese, Giuseppe, and Esposito, Franca
- Subjects
0301 basic medicine ,Biochemistry & Molecular Biology ,Programmed cell death ,Bioenergetics ,metabolism ,inflammation ,human ovarian ,cancer ,Cell Survival ,Inflammation ,Biology ,Protein Serine-Threonine Kinases ,Disease-Free Survival ,Oxidative Phosphorylation ,Immediate-Early Proteins ,03 medical and health sciences ,Multidrug Resistance Protein 1 ,Cell Line, Tumor ,medicine ,Humans ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,HSP90 Heat-Shock Proteins ,RNA, Small Interfering ,Molecular Biology ,Cisplatin ,Ovarian Neoplasms ,Original Paper ,Interleukin-6 ,Interleukin-8 ,Cancer ,11 Medical And Health Sciences ,Cell Biology ,06 Biological Sciences ,medicine.disease ,Metformin ,3. Good health ,Cell biology ,030104 developmental biology ,Apoptosis ,Drug Resistance, Neoplasm ,Cancer research ,Tumor necrosis factor alpha ,Female ,RNA Interference ,medicine.symptom ,Glycolysis ,medicine.drug - Abstract
Tumour cells have long been considered defective in mitochondrial respiration and mostly dependent on glycolytic metabolism. However, this assumption is currently challenged by several lines of evidence in a growing number of tumours. Ovarian cancer (OC) is one of the most lethal cancers worldwide, but it continues to be a poorly understood disease and its metabolic features are far to be elucidated. In this context, we investigated the role of tumour necrosis factor receptor-associated protein 1 (TRAP1), which is found upregulated in several cancer types and is a key modulator of tumour cell metabolism. Surprisingly, we found that TRAP1 expression inversely correlated with grade, stage and lower survival in a large cohort of OC patients. Accordingly, TRAP1 silencing induced resistance to cisplatin, resistant cells showed increased oxidative metabolism compared with their sensitive counterpart, and the bioenergetics cellular index of higher grade tumours indicated increased mitochondrial respiration. Strikingly, cisplatin resistance was reversible upon pharmacological inhibition of mitochondrial oxidative phosphorylation by metformin/oligomycin. At molecular level, increased oxidative metabolism in low TRAP1-expressing OC cells and tissues enhanced production of inflammatory mediators such as interleukin (IL)-6 and IL-8. Mechanistically, we identified members of the multidrug resistance complex (MDR) as key mediators of such metabolism-driven, inflammation-induced process. Indeed, treatment of OC cell lines with TNFα and IL6 induced a selective increase in the expression of TAP1 and multidrug resistance protein 1, whereas TAP1 silencing sensitized cells to cisplatin-induced apoptosis. Our results unveil a novel role for TRAP1 and oxidative metabolism in cancer progression and suggest the targeting of mitochondrial bioenergetics to increase cisplatin efficacy in human OC.Cell Death and Differentiation advance online publication, 20 May 2016; doi:10.1038/cdd.2016.39.
- Published
- 2015
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.