1. Generation and Analysis of Chromosomal Contact Maps of Yeast Species
- Author
-
Cournac, Axel, Marbouty, Martial, Mozziconacci, Julien, Koszul, Romain, Régulation spatiale des Génomes - Spatial Regulation of Genomes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), This research was supported by funding to R.K. from the European Research Council under the 7th Framework Program (FP7/2007-2013) / ERC grant agreement 260822. M.M. is the recipient of an Association pour la Recherche sur le Cancer fellowship 20100600373, European Project: 260822,EC:FP7:ERC,ERC-2010-StG_20091118,DICIG(2011), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physique Théorique de la Matière Condensée ( LPTMC ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), and European Project : 260822,EC:FP7:ERC,ERC-2010-StG_20091118,DICIG ( 2011 )
- Subjects
Chromosome conformation capture ,Genome assembly ,MESH : Chromatin ,[ SDV ] Life Sciences [q-bio] ,MESH : Chromosomes, Fungal ,MESH : Genome, Fungal ,[SDV]Life Sciences [q-bio] ,MESH : Saccharomyces cerevisiae ,Chromosome Mapping ,Saccharomyces cerevisiae ,Genome organization ,MESH: Chromosomes, Fungal ,MESH: Saccharomyces cerevisiae ,Yeast ,Chromatin ,MESH: Chromatin ,MESH: Genome, Fungal ,Chromosomes, Fungal ,Genome, Fungal ,3C ,MESH: Chromosome Mapping ,MESH : Chromosome Mapping ,3C analysis - Abstract
International audience; Genome-wide derivatives of the chromosome conformation capture (3C) technique are now well-established approaches to study the multiscale average organization of chromosomes from bacteria to mammals. However, the experimental parameters of the protocol have to be optimized for different species, and the downstream experimental products (i.e., pair-end sequences) are influenced by these parameters. Here, we describe a complete pipeline to generate 3C-seq libraries and compute chromosomal contact maps of yeast species.
- Published
- 2015