1. Complement factor H binding of monomeric C-reactive protein downregulates proinflammatory activity and is impaired with at risk polymorphic CFH variants
- Author
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Molins, Blanca, Fuentes-Prior, Pablo, Adan Civera, Alfredo, Antón, Rosa, Arostegui, Juan I., Yagüe, Jordi, Dick, Andrew D., Universitat Autònoma de Barcelona, and Universitat de Barcelona
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0301 basic medicine ,Male ,genetic structures ,Plasma protein binding ,Retinal Pigment Epithelium ,0302 clinical medicine ,Older people diseases ,Malalties de les persones grans ,Degeneració (Patologia) ,Multidisciplinary ,biology ,Molecular medicine ,Degeneration (Pathology) ,Chronic inflammation ,Inflamació ,Retinal diseases ,Chemotaxis, Leukocyte ,C-Reactive Protein ,Malalties de la retina ,Factor H ,Complement Factor H ,Cytokines ,Female ,medicine.symptom ,Protein Binding ,Down-Regulation ,Inflammation ,CCL2 ,Article ,Proinflammatory cytokine ,Cell Line ,03 medical and health sciences ,medicine ,Humans ,Genetic Predisposition to Disease ,Platelet activation ,Interleukin 8 ,Aged ,Polymorphism, Genetic ,business.industry ,C-reactive protein ,Interleukin-8 ,eye diseases ,030104 developmental biology ,Immunology ,030221 ophthalmology & optometry ,biology.protein ,Leukocytes, Mononuclear ,Wet Macular Degeneration ,sense organs ,Protein Multimerization ,business - Abstract
Inflammation and immune-mediated processes are pivotal to the pathogenic progression of age-related macular degeneration (AMD). Although plasma levels of C-reactive protein (CRP) have been shown to be associated with an increased risk for AMD, the pathophysiological importance of the prototypical acute-phase reactant in the etiology of the disease is unknown and data regarding the exact role of CRP in ocular inflammation are limited. In this study, we provide mechanistic insight into how CRP contributes to the development of AMD. In particular, we show that monomeric CRP (mCRP) but not the pentameric form (pCRP) upregulates IL-8 and CCL2 levels in retinal pigment epithelial cells. Further, we show that complement factor H (FH) binds mCRP to dampen its proinflammatory activity. FH from AMD patients carrying the “risk” His402 polymorphism displays impaired binding to mCRP and therefore proinflammatory effects of mCRP remain unrestrained.
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