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Additional file 6. Clinical performances of the K13 reference assay obtained from 565 DBS collected from P. falciparum symptomatic patients in Cambodia, Myanmar and Africa. Cells coloured in green present concordant data obtained between the K13 bMx prototype assay and the K13 reference assay; Cells coloured in pale red present discordant data obtained between the K13 bMx prototype assay and the K13 reference assay.

Additional file 2. New 3 MM filter paper format design to facilitate the semi-automated extraction protocol and avoid inter-samples DNA contamination.

Additional file 5. Classification of samples into 9 conserved groups. A. Hierarchical clustering of the 282 valid samples based on the 11-SNPs barcode. The pairwise distance between the samples is calculated as the proportion of base substitution between them over the barcode. Ward’s minimum variance method was used to build the dendrogram. The dendrogram is cut to obtain 8 clusters (k = 8). The clusters are represented by red rectangles. B. Hierarchal clustering of 282 samples based on the percentage of clustering results in which two samples are in the same cluster (when 8 clusters are considered). The clustering approach was implemented on 10,000 subsets of 230 samples each, randomly selected out of the 282 samples. Based on these 10,000 clustering results, pairwise distance between samples were calculated as the percentage of clustering results in which two samples are in the same cluster. The number of clusters (conserved groups) was selected based on the dendrogram structure. The clusters are represented by red rectangles.

Additional file 7. Clinical performances of the K13 reference assay obtained from 65 DBS collected from P. falciparum asymptomatic individuals in Cambodia. Cells coloured in green present concordant data obtained between the K13 bMx prototype assay and the K13 reference assay; Cells coloured in pale red present discordant data obtained between the K13 bMx prototype assay and the K13 reference assay.

Additional file 7. Clinical performances of the K13 reference assay obtained from 65 DBS collected from P. falciparum asymptomatic individuals in Cambodia. Cells coloured in green present concordant data obtained between the K13 bMx prototype assay and the K13 reference assay; Cells coloured in pale red present discordant data obtained between the K13 bMx prototype assay and the K13 reference assay.

Additional file 4. Principe of the Amplification-refractory mutation system (ARMS) strategy, based on allele-specific primers. In red is the mismatch position at the 3′ end of the primer. In blue an additional mismatch at the previous nucleotide.

Additional file 3. Raw data showing the results between DBS tested at IP Cambodia using an in-house protocol (QiaAmp DNA blood mini kit Qiagen and two-step real-time PCR assays targeting the Plasmodium cytochrome b gene) and at bioMérieux using the easyMAG® system (Generic 2.0.1 protocol) and the real-time PCR targeting the cytochrome c oxidase subunit 1 gene (IC) according to the origin of the DBS.

Additional file 12. Distribution of IC50 value of P. falciparum isolates per major geographic areas. Box Plot analysis is presenting median and quartiles. Dashed line figure out the threshold where parasite could be resistant for the drug (30 nM). Parasites were originating from regions distributed at the four compass points in Cambodia. ANOVA test was significant for chloroquine and mefloquine (p value = 5.62e−5 and p value = 0.0408, respectively).

Additional file 4. Principe of the Amplification-refractory mutation system (ARMS) strategy, based on allele-specific primers. In red is the mismatch position at the 3′ end of the primer. In blue an additional mismatch at the previous nucleotide.

Additional file 5. Overall clinical performances of the K13 reference assay obtained from 642 DBS collected from P. falciparum symptomatic patients in Cambodia, Myanmar and Africa and P. falciparum asymptomatic individuals in Cambodia. Cells coloured in green present concordant data obtained between the K13 bMx prototype assay and the K13 reference assay; Cells coloured in pale red present discordant data obtained between the K13 bMx prototype assay and the K13 reference assay.

Additional file 6. Clinical performances of the K13 reference assay obtained from 565 DBS collected from P. falciparum symptomatic patients in Cambodia, Myanmar and Africa. Cells coloured in green present concordant data obtained between the K13 bMx prototype assay and the K13 reference assay; Cells coloured in pale red present discordant data obtained between the K13 bMx prototype assay and the K13 reference assay.

Additional file 1. K13 bMx prototype assay 5-step workflow (24 DBS in less than 4 h).

Additional file 5. Overall clinical performances of the K13 reference assay obtained from 642 DBS collected from P. falciparum symptomatic patients in Cambodia, Myanmar and Africa and P. falciparum asymptomatic individuals in Cambodia. Cells coloured in green present concordant data obtained between the K13 bMx prototype assay and the K13 reference assay; Cells coloured in pale red present discordant data obtained between the K13 bMx prototype assay and the K13 reference assay.

Additional file 3. Raw data showing the results between DBS tested at IP Cambodia using an in-house protocol (QiaAmp DNA blood mini kit Qiagen and two-step real-time PCR assays targeting the Plasmodium cytochrome b gene) and at bioMérieux using the easyMAG® system (Generic 2.0.1 protocol) and the real-time PCR targeting the cytochrome c oxidase subunit 1 gene (IC) according to the origin of the DBS.

Additional file 12. Distribution of IC50 value of P. falciparum isolates per major geographic areas. Box Plot analysis is presenting median and quartiles. Dashed line figure out the threshold where parasite could be resistant for the drug (30 nM). Parasites were originating from regions distributed at the four compass points in Cambodia. ANOVA test was significant for chloroquine and mefloquine (p value = 5.62e−5 and p value = 0.0408, respectively).