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1. ESCMID* *This guideline was presented in part at ECCMID 2011. European Society for Clinical Microbiology and Infectious Diseases. guideline for the diagnosis and management of Candida diseases 2012: diagnostic procedures

Author

Cuenca-Estrella, M., Verweij, P. E, Arendrup, M. C, Arikan-Akdagli, S., Bille, J., Donnelly, J. P, Jensen, H. E, Lass-Flörl, C., Richardson, M. D, Akova, M., Bassetti, M., Calandra, T., Castagnola, E., Cornely, O. A, Garbino, J., Groll, A. H, Herbrecht, R., Hope, W. W, Kullberg, B. J, Lortholary, O., Meersseman, W., Petrikkos, G., Roilides, E., Viscoli, C., and Ullmann, A. J

Subjects
Microbiology (medical), Infectious Diseases, diagnosis, noncultural, guideline, Biomarkers, Candida
Abstract

As the mortality associated with invasive Candida infections remains high, it is important to make optimal use of available diagnostic tools to initiate antifungal therapy as early as possible and to select the most appropriate antifungal drug. A panel of experts of the European Fungal Infection Study Group (EFISG) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) undertook a data review and compiled guidelines for the clinical utility and accuracy of different diagnostic tests and procedures for detection of Candida infections. Recommendations about the microbiological investigation and detection of candidaemia, invasive candidiasis, chronic disseminated candidiasis, and oropharyngeal, oesophageal, and vaginal candidiasis were included. In addition, remarks about antifungal susceptibility testing and therapeutic drug monitoring were made.

Published
2012
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4. Preliminary Identification and Typing of Pathogenic and Toxigenic Fusarium Species Using Restriction Digestion of ITS1-5.8S rDNA-ITS2 Region

Author

Mirhendi, H., Ghiasian, A., Vismer, H. F., Asgary, M. R., Jalalizand, N., Arendrup, M. C., and Koichi MAKIMURA

Subjects
PCR-RFLP, Fusarium, lcsh:Public aspects of medicine, fungi, lcsh:RA1-1270, Original Article, Mycotoxins, ITS
Abstract

"nBackground: Fusarium species are capable of causing a wide range of crop plants infections as well as uncommon hu­man infections. Many species of the genus produce mycotoxins, which are responsible for acute or chronic diseases in ani­mals and humans. Identification of Fusaria to the species level is necessary for biological, epidemiological, pathologi­cal, and toxicological purposes. In this study, we undertook a computer-based analysis of ITS1-5.8SrDNA-ITS2 in 192 GenBank sequences from 36 Fusarium species to achieve data for establishing a molecular method for spe­cie-specific identification."nMethods: Sequence data and 610 restriction enzymes were analyzed for choosing RFLP profiles, and subsequently de­signed and validated a PCR-restriction enzyme system for identification and typing of species. DNA extracted from 32 refer­ence strains of 16 species were amplified using ITS1 and ITS4 universal primers followed by sequencing and restric­tion enzyme digestion of PCR products."nResults: The following 3 restriction enzymes TasI, ItaI and CfoI provide the best discriminatory power. Using ITS1 and ITS4 primers a product of approximately 550bp was observed for all Fusarium strains, as expected regarding the se­quence analyses. After RFLP of the PCR products, some species were definitely identified by the method and some strains had different patterns in same species."nConclusion: Our profile has potential not only for identification of species, but also for genotyping of strains. On the other hand, some Fusarium species were 100% identical in their ITS-5.8SrDNA-ITS2 sequences, therefore differentia­tion of these species is impossible regarding this target alone. ITS-PCR-RFLP method might be useful for preliminary differ­entiation and typing of most common Fusarium species.

5. A prospective international Aspergillus terreus survey: an EFISG, ISHAM and ECMM joint study

Author

Risslegger, Brigitte, Zoran, Tamara, Lackner, Michaela, Aigner, María, Sanchez Reus, Ferrán, Rezusta, Antonio, Chowdhary, Anuradha, Alcacer Sanchez, Juan Manuel, Taj Aldeen, Saad Jaber, Arendrup, Maiken C., Oliveri, Salvatore, Kontoyiannis, Dimitrios P., Alastruey Izquierdo, Ana, Lagrou, Katrien, Lo Cascio, Giuliana, Meis, Jacques F., Buzina, Walter, Farina, Claudio, Drogari Apiranthitou, Miranda, Grancini, Anna, Tortorano, Anna Maria, Willinger, Birgit, Hamprecht, Axel, Johnson, Elizabeth, Klingspor, Lena, Arsic Arsenijevic, Valentina, Cornely, Oliver A., Meletiadis, Joseph, Prammer, Wolfgang, Tullio, Vivian, Vehreschild, Jörg Janne, Trovato, Laura, Lewis, Russell E., Segal, Esther, Rath, Peter Michael, Hamal, Petr, Rodríguez Iglesias, Manuel, Roilides, Emmanuel, Arikan Akdagli, Sevtap, Chakrabarti, Arunaloke, Colombo, Arnaldo L., Fernández, Mariana Soledad, Martin Gomez, M. Teresa, Badali, Hamid, Petrikkos, Georgios, Klimko, Nikolai, Heimann, Sebastian M., Houbraken, Jos, Uzun, Omrum, Edlinger, Michael, de la Fuente, Sonia, Lass Flörl, Cornelia, İç Hastalıkları, Risslegger, B., Zoran, T., Lackner, M., Aigner, M., Sã¡nchez-reus, F., Rezusta, A., Chowdhary, A., Taj-aldeen, S. J., Arendrup, M. C., Oliveri, S., Kontoyiannis, D. P., Alastruey-izquierdo, A., Lagrou, K., Lo Cascio, G., Meis, J. F., Buzina, W., Farina, C., Drogari-apiranthitou, M., Grancini, A., Tortorano, A. M., Willinger, B., Hamprecht, A., Johnson, E., Klingspor, L., Arsic-arsenijevic, V., Cornely, O. A., Meletiadis, J., Prammer, W., Tullio, V., Vehreschild, J. -. J., Trovato, L., Lewis, RUSSEL EDWARD, Segal, E., Rath, P. -. M., Hamal, P., Rodriguez-iglesias, M., Roilides, E., Arikan-akdagli, S., Chakrabarti, A., Colombo, A. L., Fernã¡ndez, M. S., Martin-gomez, M. T., Badali, H., Petrikkos, G., Klimko, N., Heimann, S. M., Houbraken, J., Uzun, O., Edlinger, M., Fuente, S. De La, Lass-flã¶rl, C., Westerdijk Fungal Biodiversity Institute, and Westerdijk Fungal Biodiversity Institute - Food and Indoor Mycology

Subjects
0301 basic medicine, Antifungal Agents, Medizin, Aspergillosis, purl.org/becyt/ford/1 [https], In vitrosusceptibility, Amphotericin B, Prevalence, Aspergillus terreus, Prospective Studies, skin and connective tissue diseases, biology, General Medicine, Aspergillus terreu, 3. Good health, Crytic species, Europe, Microbiology (medical), Infectious Diseases, Aspergillus, Epidemiological Monitoring, CIENCIAS NATURALES Y EXACTAS, medicine.drug, Species complex, Surveillance study, Crytic specie, Otras Ciencias Biológicas, Aspergillosi, 030106 microbiology, Microbial Sensitivity Tests, Microbiology, Ciencias Biológicas, 03 medical and health sciences, medicine, Humans, purl.org/becyt/ford/1.6 [https], in vitro susceptibility, Amphotericin B, Aspergillosis, Aspergillus terreus, crytic species, in vitro susceptibility, biology.organism_classification, medicine.disease, bacterial infections and mycoses, 030104 developmental biology, lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
Abstract

Objectives: A prospective international multicentre surveillance study was conducted to investigate the prevalence and amphotericin B susceptibility of Aspergillus terreus species complex infections. Methods: A total of 370 cases from 21 countries were evaluated. Results: The overall prevalence of A. terreus species complex among the investigated patients with mould-positive cultures was 5.2% (370/7116). Amphotericin B MICs ranged from 0.125 to 32 mg/L, (median 8 mg/L). Conclusions: Aspergillus terreus species complex infections cause a wide spectrum of aspergillosis and the majority of cryptic species display high amphotericin B MICs. Fil: Risslegger, Brigitte. Universidad de Innsbruck; Austria Fil: Zoran, Tamara. Universidad de Innsbruck; Austria Fil: Lackner, Michaela. Universidad de Innsbruck; Austria Fil: Aigner, María. Universidad de Innsbruck; Austria Fil: Sanchez Reus, Ferrán. Hospital de la Santa Creu I Sant Pau; España Fil: Rezusta, Antonio. Universidad de Zaragoza; España Fil: Chowdhary, Anuradha. University of Delhi; India Fil: Alcacer Sanchez, Juan Manuel. Hospital de la Santa Creu I Sant Pau; Fil: Taj Aldeen, Saad Jaber. Hamad Medical Corporation; Qatar Fil: Arendrup, Maiken C.. Universidad de Copenhagen; Dinamarca Fil: Oliveri, Salvatore. Università degli Studi di Catania; Italia Fil: Kontoyiannis, Dimitrios P.. The University of Texas MD Anderson Cancer Center; Estados Unidos Fil: Alastruey Izquierdo, Ana. Universidad Carlos III de Madrid. Instituto de Salud; España Fil: Lagrou, Katrien. Katholikie Universiteit Leuven; Bélgica Fil: Lo Cascio, Giuliana. Azienda Ospedaliera Universitaria Integrata; Italia Fil: Meis, Jacques F.. Canisius Wilhelmina Hospital; Países Bajos Fil: Buzina, Walter. Medical University of Graz; Austria Fil: Farina, Claudio. ASST Papa Giovanni XXIII. Microbiology Institute; Italia Fil: Drogari Apiranthitou, Miranda. Universidad Nacional y Kapodistriaca de Atenas; Grecia Fil: Grancini, Anna. Cà Granda Ospedale Maggiore Policlinico; Italia Fil: Tortorano, Anna Maria. Università degli Studi di Milano; Italia Fil: Willinger, Birgit. Universidad de Viena; Austria Fil: Hamprecht, Axel. Universitat Zu Köln; Alemania Fil: Johnson, Elizabeth. Public Health England. Mycology Reference Laboratory; Reino Unido Fil: Klingspor, Lena. Karolinska Huddinge Hospital; Suecia Fil: Arsic Arsenijevic, Valentina. University of Belgrade; Serbia Fil: Cornely, Oliver A.. Universitat Zu Köln; Alemania Fil: Meletiadis, Joseph. Universidad Nacional y Kapodistriaca de Atenas; Grecia Fil: Prammer, Wolfgang. Klinikum Wels-Grieskirchen; Austria Fil: Tullio, Vivian. Università di Torino; Italia Fil: Vehreschild, Jörg Janne. Universitat Bonn; Alemania. Universitat Zu Köln; Alemania Fil: Trovato, Laura. Università degli Studi di Catania; Italia Fil: Lewis, Russell E.. Universidad de Bologna; Italia Fil: Segal, Esther. Tel Aviv University; Israel Fil: Rath, Peter Michael. Universitat Essen; Alemania Fil: Hamal, Petr. Universtity Hospital Olomouc; República Checa. Palacky University Olomouc; República Checa Fil: Rodríguez Iglesias, Manuel. Universidad de Cádiz; España Fil: Roilides, Emmanuel. Aristotle University School of Health Sciences; Grecia Fil: Arikan Akdagli, Sevtap. Hacettepe University; Turquía Fil: Chakrabarti, Arunaloke. Postgraduate Institute of Medical Education and Research; India Fil: Colombo, Arnaldo L.. Universidade Federal de Sao Paulo; Brasil Fil: Fernández, Mariana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martin Gomez, M. Teresa. Vall d’Hebron University Hospital; España Fil: Badali, Hamid. Mazandaran University of Medical Sciences; Irán Fil: Petrikkos, Georgios. European University Cyprus; Chipre Fil: Klimko, Nikolai. North Western State Medical University; Rusia Fil: Heimann, Sebastian M.. Universitat Zu Köln; Alemania Fil: Houbraken, Jos. Fungal Biodiversity Centre; Países Bajos Fil: Uzun, Omrum. Hacettepe University Medical School; Turquía Fil: Edlinger, Michael. Universidad de Innsbruck; Austria Fil: de la Fuente, Sonia. Hospital Ernest Lluch Martin; España Fil: Lass Flörl, Cornelia. Universidad de Innsbruck; Austria

Published
2017
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