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Additional file 1. Fig. S1: Standard curve for the quantitative-PCR assay. A standard curve of Ct values versus copy number. The standard curve was constructed by 10-fold serial dilution (8.16 × 107 to 8.16 copies) of a plasmid encoding the JEV genome. All Ct values are the mean values of quadruplicate measurements, except for the value of 8.16 copies, which is the mean value of triplicate measurements (due to the detection limit).