5 results on '"Aoi Sugiyama"'
Search Results
2. Resting Energy Expenditure in Older Inpatients: A Comparison of Prediction Equations and Measurements
- Author
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Fumiya Kawase, Yoshiyuki Masaki, Hiroko Ozawa, Manami Imanaka, Aoi Sugiyama, Hironari Wada, Ryokichi Goto, Shinya Kobayashi, and Takayoshi Tsukahara
- Subjects
Inpatients ,Nutrition and Dietetics ,resting energy expenditure ,prediction accuracy ,older patients ,indirect calorimetry ,Cross-Sectional Studies ,Infant, Newborn ,Humans ,Reproducibility of Results ,Calorimetry, Indirect ,Basal Metabolism ,Energy Metabolism ,Food Science ,Aged - Abstract
Determining energy requirements are an important component of nutritional support for patients with malnutrition; however, the validity of prediction equations for resting energy expenditure (REE) is disputed in older hospitalized patients. We aimed to assess the validity of these equations in older hospitalized patients in Japan. This was a single-center, cross-sectional study of 100 patients aged ≥70 years, hospitalized between January 2020 and December 2021. REE was measured using an indirect calorimeter and was compared to the predicted values calculated from five REE prediction equations. The mean (95% confidence interval) measured REE was 968.1 (931.0, 1005.3) kcal/day, and the mean predicted REE was higher for the FAO/WHO/UNU (1014.3 [987.1, 1041.6] kcal/day, p = 0.164) and Schofield (1066.0 [1045.8, 1086.2] kcal/day, p < 0.001) equations and lower for the Harris-Benedict (898.6 [873.1, 924.1] kcal/day, p = 0.011), Ganpule (830.1 [790.3, 869.9] kcal/day, p < 0.001), and body weight (kg) × 20 (857.7 [821.9, 893.5] kcal/day, p < 0.001) equations. In the age group analysis, none of the predicted values were within a 10% error for more than 80% of patients aged 70–89 years and ≥90 years. The five REE prediction equations did not provide accurate estimates. Validated REE prediction equations need to be developed for older hospitalized patients.
- Published
- 2022
3. Structural comparison of the C-terminal domain of functionally divergent lyssavirus P proteins
- Author
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Gregory W. Moseley, Tomo Nomai, Aoi Sugiyama, Miku Minami, Katsumi Maenaka, T. Ose, Xinxin Jiang, Naoto Ito, Paul R. Gooley, and Min Yao
- Subjects
Models, Molecular ,Protein Conformation ,viruses ,Biophysics ,Mokola virus ,Crystallography, X-Ray ,medicine.disease_cause ,Biochemistry ,Virus ,Viral Proteins ,Protein Domains ,Rhabdoviridae Infections ,medicine ,Humans ,Molecular Biology ,Lyssavirus ,Genetics ,biology ,Chemistry ,C-terminus ,Rabies virus ,Cell Biology ,biology.organism_classification ,Duvenhage virus ,CTD ,Hydrophobic and Hydrophilic Interactions ,Function (biology) - Abstract
Lyssavirus P protein is a multifunctional protein that interacts with numerous host-cell proteins. The C-terminal domain (CTD) of P is important for inhibition of JAK-STAT signaling enabling the virus to evade host immunity. Several regions on the surface of rabies virus P are reported to interact with host factors. Among them, an extended, discrete hydrophobic patch of P CTD is notable. Although structures of P CTD of two strains of rabies virus, and of mokola virus have been solved, the structure of P CTD for Duvenhage virus, which is functionally divergent from these species for immune evasion function, is not known. Here, we analyze the structures of P CTD of Duvenhage and of a distinct rabies virus strain to gain further insight on the nature and potential function of the hydrophobic surface. Molecular contacts in crystals suggest that the hydrophobic patch is important to intermolecular interactions with other proteins, which differ between the lyssavirus species.
- Published
- 2020
4. The Measles Virus V Protein Binding Site to STAT2 Overlaps That of IRF9
- Author
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Xinxin Jiang, Gregory W. Moseley, Shinya Saijo, Takuya Wakahara, Aoi Sugiyama, Takashi Tadokoro, Paul R. Gooley, Toyoyuki Ose, Madoka Kimoto, Katsumi Maenaka, Min Yao, Nobutaka Shimizu, Yuma Nagano, Yasuyo Noguchi, and Nana Yabuno
- Subjects
recombinant-protein production ,Immunology ,Protein domain ,protein-protein interactions ,Gene Expression ,Plasma protein binding ,Microbiology ,Protein–protein interaction ,Measles virus ,Viral Proteins ,03 medical and health sciences ,paramyxovirus ,Protein Domains ,zinc finger proteins ,protein purification ,Virology ,Humans ,measles ,structural biology ,Protein Interaction Domains and Motifs ,STAT1 ,STAT transcription factors ,STAT2 ,innate immunity ,Janus Kinases ,immune evasion ,030304 developmental biology ,0303 health sciences ,Binding Sites ,biology ,Structure and Assembly ,030302 biochemistry & molecular biology ,STAT2 Transcription Factor ,Zinc Fingers ,Phosphoproteins ,biology.organism_classification ,Immunity, Innate ,Interferon-Stimulated Gene Factor 3, gamma Subunit ,Cell biology ,STAT1 Transcription Factor ,Structural biology ,Insect Science ,biology.protein ,STAT protein ,Protein Binding ,Signal Transduction - Abstract
Measles virus (MeV) is a highly immunotropic and contagious pathogen that can even diminish preexisting antibodies and remains a major cause of childhood morbidity and mortality worldwide despite the availability of effective vaccines. MeV is one of the most extensively studied viruses with respect to the mechanisms of JAK-STAT antagonism. Of the three proteins translated from the MeV P gene, P and V are essential for inactivation of this pathway. However, the lack of data from direct analyses of the underlying interactions means that the detailed molecular mechanism of antagonism remains unresolved. Here, we prepared recombinant MeV V protein, which is responsible for human JAK-STAT antagonism, and a panel of variants, enabling the biophysical characterization of V protein, including direct V/STAT1 and V/STAT2 interaction assays. Unambiguous direct interactions between the host and viral factors, in the absence of other factors such as Jak1 or Tyk2, were observed, and the dissociation constants were quantified for the first time. Our data indicate that interactions between the C-terminal region of V and STAT2 is 1 order of magnitude stronger than that of the N-terminal region of V and STAT1. We also clarified that these interactions are completely independent of each other. Moreover, results of size exclusion chromatography demonstrated that addition of MeV-V displaces STAT2-core, a rigid region of STAT2 lacking the N- and C-terminal domains, from preformed complexes of STAT2-core/IRF-associated domain (IRF9). These results provide a novel model whereby MeV-V can not only inhibit the STAT2/IRF9 interaction but also disrupt preassembled interferon-stimulated gene factor 3. IMPORTANCE To evade host immunity, many pathogenic viruses inactivate host Janus kinase signal transducer and activator of transcription (STAT) signaling pathways using diverse strategies. Measles virus utilizes P and V proteins to counteract this signaling pathway. Data derived largely from cell-based assays have indicated several amino acid residues of P and V proteins as important. However, biophysical properties of V protein or its direct interaction with STAT molecules using purified proteins have not been studied. We have developed novel molecular tools enabling us to identify a novel molecular mechanism for immune evasion whereby V protein disrupts critical immune complexes, providing a clear strategy by which measles virus can suppress interferon-mediated antiviral gene expression.
- Published
- 2020
5. Fabrication of amorphous bulk and multi-phase ceramics by melting method in the HfO2-Al2O3-Gd2O3-Eu2O3system
- Author
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Aoi Sugiyama, Naonori Sakamoto, Tomoaki Watanabe, Masahiro Yoshimura, and Shunji Araki
- Subjects
Nanocomposite ,Materials science ,Oxide ,Mineralogy ,Sintering ,Condensed Matter Physics ,Ceramic matrix composite ,Electronic, Optical and Magnetic Materials ,Amorphous solid ,chemistry.chemical_compound ,chemistry ,Mechanics of Materials ,visual_art ,Materials Chemistry ,Ceramics and Composites ,Aluminium oxide ,visual_art.visual_art_medium ,Ceramic ,Electrical and Electronic Engineering ,Composite material ,Ternary operation - Abstract
Ceramics have generally been fabricated from powders by shape forming & sintering methods except for glasses and glass ceramics. Glasses and glass ceramics can be fabricated by melting methods. The melting method has not only higher productivity but also higher shape forming ability than powder processes via forming & sintering methods. Thus we have reinvestigated melting methods in binary and ternary oxides systems to fabricate amorphous bulk ceramics and bulk nano composites. We have successfully fabricated amorphous phases by simple melt solidification methods in ternary eutectic melts in the HfO2-Al2O3-Gd2O3system. The present study demonstrates the formation of the amorphous phases in quaternary systems HfO2-Al2O3-Gd2O3-Eu2O3. Furthermore, we have also succeeded to fabricate nano-structured bulk ceramics, which consisted of constituent oxide grains with 20–100 nm in size, by post annealing of the amorphous phase.
- Published
- 2006
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