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Objectives: This study aimed to identify disease-related markers in persistent apical periodontitis (PAP) biopsies and examine whether these were associated with comorbidities. Materials and method: The levels of the cytokines GM-CSF, IFN-γ, IL-2, IL-6, IL-9, IL-10, IL-13, IL-15, IL-17E/IL-25, IL-21, IL-23, IL-27, IL-28A/IFN -λ2, IL-33, MIP-3α/CCL20 and TNF-α were determined in lesions from patients with persistent apical periodontitis (n = 20) and compared to healthy bone samples (n = 20). Results: We identified eleven cytokines to be differently expressed, and among them, IL-2, IL-6, IL-17E, IL-21, and IL-27 appeared to drive the discrepancy between the disease and healthy groups. The levels of T follicular helper (Tfh) cell promoting cytokines (IL-21, IL-6, IL-27) were enhanced while T helper (Th) 1 cell promoting cytokine (IL-2), Th2 cell promoting cytokine (IL-13), and Th17 cell promoting cytokine (IL-17E) were reduced in the PAP group. The data also indicate that Tfh cell differentiation (IL-21), along with Th1 (GM-CSF, IFNγ), Th2 (IL-13), and Th17 (GM-CSF) cell differentiation, might be increased in the subpopulation of patients suffering from rheumatoid arthritis, whereas no differences were found in patients with cardiovascular diseases. Conclusions: A cluster of potential cellular markers for PAP was identified. The lesions contained additional factors that might be associated with differentiation of various T cell populations. Clinical Relevance: Molecular analyses of PAP may result in identification of prognostic markers.

Background Neurological manifestations in patients with COVID-19 have been reported previously as outcomes of the infection. The purpose of current study was to investigate the occurrence of neurological signs and symptoms in COVID-19 patients, in the county of Östergötland in southeastern Sweden. Methods This is a retrospective, observational cohort study. Data were collected between March 2020 and June 2020. Information was extracted from medical records by a trained research assistant and physician and all data were validated by a senior neurologist. Results Seventy-four percent of patients developed at least one neurological symptom during the acute phase of the infection. Headache (43%) was the most common neurological symptom, followed by anosmia and/or ageusia (33%), confusion (28%), hallucinations (17%), dizziness (16%), sleep disorders in terms of insomnia and OSAS (Obstructive Sleep Apnea) (9%), myopathy and neuropathy (8%) and numbness and tingling (5%). Patients treated in the ICU had a higher male presentation (73%). Several risk factors in terms of co-morbidities, were identified. Hypertension (54.5%), depression and anxiety (51%), sleep disorders in terms of insomnia and OSAS (30%), cardiovascular morbidity (28%), autoimmune diseases (25%), chronic lung diseases (24%) and diabetes mellitus type 2 (23%) founded as possible risk factors. Conclusion Neurological symptoms were found in the vast majority (74%) of the patients. Accordingly, attention to neurological, mental and sleep disturbances is warranted with involvement of neurological expertise, in order to avoid further complications and long-term neurological effect of COVID-19. Furthermore, risk factors for more severe COVID-19, in terms of possible co-morbidities that identified in this study should get appropriate attention to optimizing treatment strategies in COVID-19 patients.

Nuestro objetivo político es aumentar la participación de los ciclistas y, al mismo tiempo, reducir el número de ciclistas heridos, y lo estamos haciendo bien. Lesiones y muertes de tráfico son un gran costo para la sociedad y causa dolor en las víctimas, por lo que la seguridad del ciclista es importante para nosotros

A complexation chemistry model is applied for the study of chelating ion exchange systems. A method is presented for the determination of equilibrium constants of the metal chelates with the chelating resin Dowex A-1. It is based on simultaneous pM and pH measurements in optimal pH ranges using ion selective electrodes. Equilibrium constants were determined for 1:1 calcium(II), copper(II), lead(II) and mercury(II) chelates with the resin. Theoretical distribution coefficients were calculated as function of pH for respective metal ion resin systems from the equilibrium constants determined.