Search

Your search keyword '"Andreeva, L."' showing total 50 results
Start Over Author "Andreeva, L." Language undetermined
50 results on '"Andreeva, L."'

6. Evenly tritium-labeled peptides and their in vivo and in vitro biodegradation

Author

Zolotarev, I. A., Dadaian, A. K., Dolotov, O. V., Kozik, V. S., Kost, N. V., Sokolov, O. I., Dorokhova, E. M., Meshavkin, V. K., Inozemtseva, L. S., Marina Gabaeva, Andreeva, L. A., Alfeeva, L. I., Pavlov, T. S., Badmaeva, K. E., Badmaeva, S. E., Bakaeva, Z. V., Kopylova, G. N., Samonina, G. E., Vas Kovskiǐ, B. V., Grivennikov, I. A., Zozulia, A. A., and Miasoedov, N. F.

Subjects
Hydrolysis, Brain, Enkephalins, In Vitro Techniques, Tritium, Aminopeptidases, Peptide Fragments, Rats, Rats, Sprague-Dawley, Adrenocorticotropic Hormone, Isotope Labeling, Animals, Neprilysin, Oligopeptides, Chromatography, High Pressure Liquid, Enkephalin, Leucine
Abstract

Biologically active peptides evenly labeled with tritium were used for studying the in vitro and in vivo biodegradation of the peptides. Tritium-labeled peptides with a specific radioactivity of 50-150 Ci/mmol were obtained by high temperature solid phase catalytic isotope exchange (HSCIE) with spillover tritium. The distribution of the isotope label among all amino acid residues of these peptides allows the simultaneous determination of practically all possible products of their enzymatic hydrolysis. The developed analytical method includes extraction of tritium-labeled peptides from organism tissues and chromatographic isolation of individual labeled peptides from the mixture of degradation products. The concentrations of a peptide under study and the products of its biodegradation were calculated from the results of liquid scintillation counting. This approach was used for studying the pathways of biodegradation of the heptapeptide TKPRPGP (Selank) and the tripeptide PGP in blood plasma. The pharmacokinetics of Selank, an anxiolytic peptide, was also studied in brain tissues using the intranasal in vivo administration of this peptide. The concentrations of labeled peptides were determined, and the pentapeptide TKPRP, tripeptide TKP, and dipeptides RP and GP were shown to be the major products of Selank biodegradation. The study of the biodegradation of the heptapeptide MEHFPGP (Semax) in the presence of nerve cells showed that the major products of its biodegradation are the pentapeptide HFPGP and tripeptide PGP. The enkephalinase activity of blood plasma was studied with the use of evenly tritium-labeled [Leu]enkephalin. A high inhibitory effect of Semax on blood plasma enkephalinases was shown to arise from its action on aminopeptidases. The method, based on the use of evenly tritium-labeled peptides, allows the determination of peptide concentrations and the activity of enzymes involved in their degradation on a tg scale of biological samples both in vitro and in vivo.

22. Role of gp130 cytokine receptor in growth and differentiation of normal and malignant hematopoietic cells. II. Monoclonal antibodies to interleukin 6 in the treatment of multiple myeloma

Author

Votyakova, O. M., Tupitsyn, N. N., Osmanov, D. Sh, Demina, E. A., Andreeva, L. Yu, Ptushkin, V. V., Larionova, V. B., Turnyanskaya, E. G., Pavlovskaya, A. I., Molodykh, A. A., Lyubimova, P. V., Rodionova, L. M., Zimina, O. G., Poltorakin, I. A., Nikolay Zhukov, Rossi, J. -F, Brochier, J., and Wijdenes, J. N. N.

38. Superhigh-dosage chemotherapy with the transplantation of autologous hemopoietic precursor cells in patients with a prognostically unfavorable relapse and resistant course of lymphogranulomatosis | Sverkhvysokodoznaia khimioterapiia s transplantatsiei autologichnykh kletok predshestvennikov gemopoéza u bol'nykh s prognosticheski neblagopriiatnym retsidivom i rezistentnym techeniem limfogranulematoza

Author

Ptuskin, V. V., Uss, A. L., Demina, E. A., Chervonobab, I. V., Milanovich, N. F., Nikolai Tupitsyn, Larionova, V. B., Batan, Z. E., Kondrat Eva, N. E., Zmachinskii, V. A., Andreeva, L. A., Snegir, V. M., Mkheidze, D. M., and Chimishkian, K. L.

Catalog

Books, media, physical & digital resources
See catalog results