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Mucoepidermoid carcinoma (MEC) of the breast is an extremely rare salivary gland-type tumor characterized by epidermoid, basaloid, intermediate, and/or mucinous cells arranged in solid and cystic patterns. Despite their triple-negative phenotype, breast MECs are generally considered low-risk malignancies but their biology is largely unexplored; therefore, guidelines for clinical management are lacking. Here, we sought to characterize the molecular landscape of breast MECs. Thirteen cases were histologically reviewed, characterized for tumor-infiltrating lymphocytes (TILs), and were subjected to immunohistochemistry for programmed death-ligand 1 (PD-L1, clone 22C3), EGFR, and amphiregulin (AREG). Rearrangements in MAML2 and EWSR1 were investigated by fluorescent in situ hybridization. Targeted next-generation sequencing of 161 genes was performed on eight cases. Most MECs had low histological grade (n = 10, 77%), with the presence of TILs (n = 9/12; 75%) and PD-L1 combined positive score ranging from 10 to 20 (n = 4/6; 67%). All cases showed EGFR and AREG overexpression and were fusion negative. Enrichment of genetic alterations was observed in PI3K/AKT/mTOR and cell cycle regulation pathways, while only one case harbored TP53 mutations. This is the first study providing extensive molecular data on breast MECs and the largest collection of cases available to date in the literature. Breast MECs lack TP53 mutations found in high-grade forms of triple-negative breast cancers and MAML2 or EWSR1 rearrangements pathognomonic of salivary MECs. Triple-negativity and PD-L1 positivity suggest a window of opportunity for immunotherapy in these patients. The EGFR/AREG axis activation, coupled with the mutational patterns in PI3K/AKT/mTOR and cell cycle pathways warrants caution in considering MECs as low-risk neoplasms.

ObjectiveBecause most available treatments for managing seasonal allergic rhinitis show some side effects without reducing recurrence, natural anti-allergic products could represent an interesting treatment addition. This study aimed to analyse the efficacy and tolerance of quail egg as adjunctive therapy in seasonal allergic rhinitis.MethodIn a Consolidated Standards of Reporting Trials compliant framework, patients with seasonal allergic rhinitis were prospectively randomised to receive mometasone nasal spray for four weeks or the same topical corticosteroid therapy plus commercially available oral quail egg and zinc tablets.ResultsForty patients were enrolled. The mometasone + quail egg and zinc tablets group showed a greater reduction in nasal itching, sneezing and total nasal symptom scores than the mometasone nasal spray only group. A higher proportion of participants in the mometasone + quail egg and zinc tablets group had good rhinitis control than in the mometasone nasal spray only group, with no need for rescue medications.ConclusionDespite the need for a further larger study, quail egg preliminarily appears to be an effective adjunct to topical steroid therapy in seasonal allergic rhinitis.

The precious coral Corallium rubrum (Linnaeus, 1758) is a charismatic Mediterranean species. A recent large-scale investigation along the Italian coast highlighted its widespread occurrence at mesophotic and upper bathyal depths, especially on coralligenous and bathyal vertical hardgrounds. The lack of morphometric data limited the considerations on the structure and health status of the populations, fundamental to identify the most vulnerable sites and the correct management actions. For this reason, a ROV dataset, consisting of 624 dives carried out between 40 m and 1825 m in the Ligurian Sea, Tyrrhenian Sea, and Sicily Channel, was analysed to extrapolate quantitative data to describe the populations. Ten random frames were obtained from each of the 170 sites hosting red coral. Density, height, and entanglement were evaluated for about 15700 colonies counted in the frames. The densest populations were mainly found between 40 m and 80 m, with a clear latitudinal density decrease. The mesophotic populations were characterized by both scattered and densely aggregated colonies, while the bathyal ones were dominated by sparse colonies. This study identified 17 major coral areas based on the geographic proximity of the sites hosting red coral and their topographic and oceanographic affinity. The size-frequency distribution of the heights was skewed towards the smaller classes in almost all populations, with a modal class between 2 cm and 4 cm. This study depicted a stress situation of the populations throughout the entire study area, which could be correlated also to the long-term harvesting pressure carried out in the basins. Two additional sources of direct mortality were pointed out in this study. Entanglement to artisanal and recreational fishing gear interested about 18% of the recorded colonies, mainly at mesophotic depths. Almost all populations suffered from mechanical entanglement, with the highest percentages in the Ligurian Sea and Sicilian areas. This study also highlighted a massive occurrence of recent deep mortality events, mainly along the eastern and southern coast of Sardinia and in the Campanian Archipelago. Thirty sites with extensive patches of dead colonies still in place were reported from 70 m to around 200 m, but their formation remains unclear.

This is the first paper to provide detailed information on the age and growth ofTrachinotus ovatus. The size of the 244 individuals collected in the Strait of Messina ranged from 2.7 to 30.4 cm in fork length (LF) and 0.31 to 508.6 g in body mass (M). The relationship between these parameters (M-LF) was investigated and showed a good fit. Age estimation based on vertebrae and otoliths yielded similar results, suggesting a maximum age of five years. However, the precision and accuracy tests, such as percentage of agreement (PA), mean coefficient of variation (ACV) and average percent error (APE) indicated that the otolith readings (97.83% PA, 0.54% ACV and 0.38% APE) were more reliable for age estimation than vertebrae readings (82.17% PA, 5.33% ACV and 3.77% APE). The multi-model inference approach allowed us to compare different non-linear growth models. The von Bertalanffy model (L∞=29.139,k=0.496 andt0=−0.347) fitted the length-at-age data best. This species has a relatively rapid growth and an estimated longevity of five to seven years. This information could be used for management and first stock assessment studies onT. ovatusin the Mediterranean Sea., Este es el primer artículo que proporciona información detallada sobre la edad y el crecimiento deTrachinotus ovatus. El tamaño de los 244 individuos recolectados en el Estrecho de Messina osciló entre 2.7 y 30.4 cm de longitud a la horquilla (LF) y 0.31 a 508.6 g de masa corporal (M). Se investigó la relación entre estos parámetros (M-LF) y mostró un buen ajuste. La estimación de la edad basada en vértebras y otolitos arrojó resultados similares, lo que sugiere una edad máxima de cinco años. Sin embargo, las pruebas de precisión y exactitud, como el porcentaje de concordancia (PA), el coeficiente medio de variación (ACV) y el porcentaje de error medio (APE), indicaron que las lecturas del otolito (97.83% PA, 0.54% ACV y 0.38 % APE) fueron más confiables para la estimación de la edad que las lecturas de vértebras (82.17% PA, 5.33% ACV y 3.77% APE). El enfoque de inferencia de modelos múltiples nos permitió comparar diferentes modelos de crecimiento no lineal. El modelo de von Bertalanffy (L∞=29.139,k=0.496 andt0=−0.347) se ajusta mejor a los datos de talla por edad. Esta especie tiene un crecimiento relativamente rápido y una longevidad estimada de cinco a siete años. Esta información podría utilizarse para la ordenación y los primeros estudios de evaluación de poblaciones deT. ovatusen el mar Mediterráneo.

SUMMARY: Background: This retrospective study aimed to assess whether the use of natural foods, also in combination with enteral nutrition, improves rehabilitative recovery in patients severe acquired brain injuries. Methods: 40 severe acquired brain injuries patients (75% males, aged 50.2 ± 16.6) were selected. The study population was divided into three groups based on the type of nutrition administered (enteral, oral, and mixed). Mini Nutritional Assessment and Level of Cognitive Functioning scales were used to assess changes from admission to discharge in each group. Multiple logistic regression model was performed to assess the association between the feeding typology and nutritional recovery. Results: At admission, about 97.5% of patients were malnourished. We found significant score changes from baseline to follow-up for both test used and in each subgroup, except for the subgroup that includes patients fed with enteral nutrition. There were significant differences in biochemical indicators, including levels of albumin (p

Background Integrating unmanned aerial vehicles (UAV) as a new method of pesticide application into existing commercial crop protection systems requires extensive research and comparison to conventional, proven application technology. Pest control expressed as efficacy against target pests, and spray quality expressed as coverage and chemical residue are three key criteria. We investigated and compared these quantitative parameters between a multi-rotor UAV and conventional piloted airplanes in two commercial alfalfa production systems. Results Effective and equivalent control of leaf-feeding insect pests was achieved by both methods of aerial application when delivering chlorantraniliprole at the same labeled use rate in different spray volumes (46.8 and 93.5 L/ha) on commercially grown alfalfa in California. Residue levels and spray coverage were also comparable and consistent between the UAV and airplane applications across three sampling techniques, specifically residue levels on alfalfa, insecticide recovery from filter paper, and spray coverage on water sensitive cards. Differences in droplet size and deposit characteristics were more variable for the UAV than airplanes based on analysis of deposition images. Conclusion The results of this study provide confidence supporting the use of small-scale multi-rotor UAVs for pesticide application on agricultural crops. According to the parameters tested, UAV application quality and crop protection performance were comparable to that of the conventional fixed wing airplane application. However, the droplet spectrum and the short-term fate of droplets from unmanned aerial spray system require further optimization for effective and efficient crop protection with minimal risk to the environment.

The Coronavirus disease (COVID-19) pandemic has had a strong impact on eating behavior. To maintain health and well-being, correct nutrition is essential, especially when the immune system is under pressure.The aim of this study was to evaluate the correlation between emotional aspects and the eating behavior of a group of people living in Sicily during the lockdown due to COVID-19.We used a cross-sectional survey design with an anonymous online questionnaire, administered through technological means.During the COVID-19 pandemic, 91 participants experienced high stress (PSS = 18.14 ± 4.1), and eating was associated with emotional behaviors (DEBQ EM = 41.74 ± 10.9). Stress correlated positively with the emotional and external aspects of eating behaviors.The present study showed that the COVID-19 lockdown caused high stress with a worsening in eating behaviors. The study participants had difficulty in adequately coping with some emotions and feelings, developing an 'addiction' to food.

Introduction. Learning disabilities are due to genetic and/or neurobiological factors that alter brain functioning by affecting one or more cognitive processes related to learning. This study aimed to assess the efficacy of cognitive telerehabilitation in adolescents with learning disabilities to overcome the treatment problems related to the COVID-19 pandemic lockdown. Methods. Twenty-four patients diagnosed with Unspecified Learning Disability (mean ± SD age: 18.2 ± 2.9 years; 50% male) were enrolled in this study. The patients were assessed by a neuropsychological evaluation at the beginning and at the end of the program. All patients received cognitive treatment via a specific telehealth app to stimulate the cognitive skills related to learning. The treatment lasted four weeks, with a daily training (lasting up to 60 min), for five days a week. Results. Our young patients reported a statistically significant improvement in the main cognitive domains that are usually compromised in learning disabilities, including sustained and selective attention, shifting of attention, control of interference, memory and speed of information processing. Moreover, the patients showed a good usability and motivation during the training. Conclusions. Our study has shown that telerehabilitation could be a valid tool for the rehabilitation of specific cognitive skills in adolescents with learning difficulties.

Environmental climate change has encouraged countries across the world to develop policies aimed to the reduction in energy consumption and greenhouse gas emissions. The introduction of Zero-Emission Vehicles based on electrical powertrains, could reduce the emission of environmental pollutants, the noise levels and could increase the liveability of urban areas. Although in recent years research on batteries has brought several benefits to electric vehicle performance, key barriers to their adoption are still high cost, reduced autonomy, long charging times and the leak of a suitable network of charging stations. Substantial improvements in electric vehicles performance are expected with the development of new Li-ion batteries, thanks to some notable advantages over other types of batteries, such as: high energy density, high power density, long cycle life and long calendar life. This paper is aimed to present a reliability assessment procedure based on an ageing model able to estimate from datasheet information the lifetime of Lithium-ion batteries for electric vehicles, the residual capacity and reliability margins under different driving cycles, taking also into account the battery calendar ageing.

Morquio B disease is an attenuated phenotype within the spectrum of beta galactosidase (GLB1) deficiencies. It is characterised by dysostosis multiplex, ligament laxity, mildly coarse facies and heart valve defects due to keratan sulphate accumulation, predominantly in the cartilage. Morquio B patients have normal neurological development, setting them apart from those with the more severe GM1 gangliosidosis. Morquio B disease, with an incidence of 1:250.000 to 1:1.000.000 live births, is very rare. Here we report the clinical-biochemical data of nine patients. High amounts of keratan sulfate were detected using LC-MS/MS in the patients' urinary samples, while electrophoresis, the standard procedure of qualitative glycosaminoglycans analysis, failed to identify this metabolite in any of the patients' samples. We performed molecular analyses at gene, gene expression and protein expression levels, for both isoforms of the GLB1 gene, lysosomal GLB1, and the cell-surface expressed Elastin Binding Protein. We characterised three novel GLB1 mutations [c.75 + 2 T > G, c.575A > G (p.Tyr192Cys) and c.2030 T > G (p.Val677Gly)] identified in three heterozygous patients. We also set up a copy number variation assay by quantitative PCR to evaluate the presence of deletions/ insertions in the GLB1 gene. We propose a diagnostic plan, setting out the specific clinical- biochemical and molecular features of Morquio B, in order to avoid misdiagnoses and improve patients' management.

Background Hydrothermal vents, cold seeps, pockmarks and seamounts are widely distributed on the ocean floor. Over the last fifty years, the knowledge about these volcanic-associated marine ecosystems has notably increased, yet available information is still limited, scattered, and unsuitable to support decision-making processes for the conservation and management of the marine environment. Methods Here we searched the Scopus database and the platform Web of Science to collect the scientific information available for these ecosystems in the Mediterranean Sea. The collected literature and the bio-geographic and population variables extracted are provided into a systematic map as an online tool that includes an updated database searchable through a user-friendly R-shiny app. Results The 433 literature items with almost one thousand observations provided evidence of more than 100 different volcanic-associated marine ecosystem sites, mostly distributed in the shallow waters of the Mediterranean Sea. Less than 30% of these sites are currently included in protected or regulated areas. The updated database available in the R-shiny app is a tool that could guide the implementation of more effective protection measures for volcanic-associated marine ecosystems in the Mediterranean Sea within existing management instruments under the EU Habitats Directive. Moreover, the information provided in this study could aid policymakers in defining the priorities for the future protection measures needed to achieve the targets of the UN Agenda 2030.

In this paper, a live hectocotylus, found inside a stranded female Argonauta argo shell, allowed us to describe the behaviour of this copulatory arm and add new information on its morphology. The hectocotylus penile filament is contained in a special membranous pocket or sac, almost completely transparent, extending from the apex of the basal part to about one-third of the central section. The hectocotylus assumes a folded position to protect the penile filament in the membranous sac. This is probably extracted only when copulation occurs. Our observations seem to suggest that the shell may have the secondary function of storing and protecting the hectocotyli received by males until the copulation. The observations confirmed the high vitality and endurance of the hectocotylus after detachment from the A. argo male.

We describe the interaction between harpoon fishing activity and sharks, which opportunistically depredated harpoon catches in the Strait of Messina. Shark bite marks were observed on harpooned swordfish during the period 2014–2020, with different damages to the catches. Most of the depredation events have focused on large swordfish, generally weighing more than 60 kg. Data on direct observations were implemented by interviews and questionnaires to fishermen aimed to recover the information on their local fishing and ecological knowledge. Fishermen provided additional data on shark-harpoon fishing interactions also supplying information on by-catch species (i.e., bluefin tuna). Therefore, these results suggest that sharks migrating through the Strait of Messina are occasionally attracted by injured prey, due to their ability to detect chemical cues, fish distress stimuli and body fluids (i.e. blood) in the water. In addition, our investigations showed an increase in shark attacks on harpooned fish over time, likely due to an increase in harpoon swordfish catches. This may be related to the effects of the driftnets’ ban enforced by European Regulations in the last decades.

Fishing leads to drastic changes in ecosystems with a net loss of predatory biomass. This issue has been evidenced from historical ecological studies and from the evaluation of the effects of effective and large marine protected areas. In two fishery reserves off the northern Sicily coast the red mullet Mullus barbatus underwent an impressive biomass increment and a few piscivores fish species recovered after a trawl ban. The red mullet, more than 20% of all demersal fish in the untrawled areas, represented a huge food resource to its potential predators. By contrasting two trawled and two untrawled gulfs we figured out predator - prey relationships through the use of a combined approach based on trophic niche, stable isotopes data and biomass. We show that the white grouper Epinephelus aeneus and the black-bellied anglerfish Lophius budegassa are the most important predators of the red mullet with higher biomass in the untrawled areas. We also found a potential re-establishment of the trophic role for white grouper inside the untrawled areas. Our study highlights some of the benefits obtained from the use of large offshore marine protected areas as a tool for ecosystem restoration.

Purpose Many studies have evaluated the characteristics of insight, especially in psychiatric patient populations. However, this construct has been poorly examined within neurological disorders. We explored the relationship between altered insight, mood disorders and neurocognitive functioning in a sample of patients admitted to a neurological rehabilitation unit. Method Each patient, at the time of hospitalization (T0), underwent an evaluation of the overall cognitive profile, of the frontal functions, and the compilation of scales aimed at investigating the 4 domains under examination of insight and anxiety-depressive symptomatology. After 3 months (T1), at the end of the rehabilitative and supportive treatment, the patients underwent the same evaluation performed at T0. Results Our results showed significant differences between T0 and T1 in the variables examined related to insight. In particular, there was a correlation between the global cognitive profile, including executive functions, and all insight domains. This confirms how the degree of cognitive deficit, especially of executive type, affects all levels of awareness of the individual. We have also found correlations between mood disorders and insight. In particular, our results show that depression versus anxiety plays a fundamental role in a person's awareness. Conclusions The study of insight is fundamental not only for the relapses it could have on the patient, but also on those to health care professionals. In fact, having an adequate insight could lead to a greater motivation of the patient to be more complimentary to pharmacological and rehabilitative therapies, also favoring social reintegration.

Background. Multiple sclerosis (MS) is an autoimmune and demyelination disease of the central nervous system that causes progressive accumulation of disability over time. Recent studies have highlighted the correlation between metabolic disorders and cognitive dysfunctions. The present study aims to evaluate the correlation between components of the lipid profile and cognitive dysfunctions in patients affected by MS.Methods. 90 MS inpatients were included in this study. We divided the sample into three subgroups to evaluate the influence of the presence of dyslipidemia: G1 (patients with dyslipidemia), G2 (patients without dyslipidemia), G3 (patients with a higher than normal lipid value). Patients underwent rehabilitation treatment which included conventional physiotherapy, speech therapy, psychological support, cognitive rehabilitation, nutritional therapy, robotic rehabilitation, cognitive rehabilitation, and virtual reality.Results. The results showed that the three subgroups had a significant improvement in global cognitive functioning (MOCA p

In this paper a rare case of a double swordfish mortal attack against an adult blue shark (Prionace glauca) is reported. A female blue shark, with a total length of 3 m, was found stranded along the southern Sicilian coast (Strait of Sicily, Mediterranean Sea) on 30 May 2018. The analysis of this carcass revealed the presence of two swordfish bill fragments, impaled in the shark head; the former on the snout, the latter near the eye. The results of anatomical and computed tomography scanning analysis on the head of the blue shark showed that the larger bill fragment (19.7 cm) probably determined the death of this animal, having been impaled in a vital point, just behind the right eye. The analysis of both these events and other similar swordfish-shark interactions reported in the literature makes possible the hypothesis that young swordfish specimens put in place a precise defensive strategy against their potential predators or competitors, aimed at hitting vulnerable and vital points and delivering a mortal blow.

Energy efficiency in the building sector plays a key role in supporting European and global commitments against the current climate crisis. A massive adoption of deep renovation measures would allow a global reduction of energy need up to 36%, based on estimations. However, the market for building renovation is still limited, due to uncertainties associated with risk evaluation. This paper aims to suggest a method to evaluate the financial impacts of technical risks related to energy efficiency investments. Key performance indicators (KPIs) necessary to evaluate the investment risk associated with energy renovation have been defined based on an analysis of the correlation between technical and financial risks, and their originating factors or root causes. The evaluation has been carried out thanks to the EEnvest tool: a web-based search and match platform, developed within the EEnvest collaborative research project funded by the European Commission (EC). This evaluation methodology has then been applied to a case study, an office building located in Rome, for whom an energy efficient renovation project was already in place to reduce energy needs. The investment risk of the renovation project is calculated for two different scenarios: with and without risk mitigation measures being applied during the design, installation and operation phases. The results show the different technical and financial risk trends of these two scenarios, highlighting the benefits obtained by the implementation of mitigation measures.

Limb apraxia is an acquired cognitive-motor disorder characterized by spatial and temporal disorganization of limb movements, negatively affecting the quality of life of patients, including those with multiple sclerosis (MS). Although recent studies have shown the potential role of VR in increasing cognitive and motor functions, only a few studies have been carried out on the rehabilitation of upper limb apraxia. Hence, our study aims to evaluate the potential efficacy of VR training to improve upper limb ideomotor apraxia in patients with MS.One hundred and six patients, affected by secondary progressive MS, who attended our Robotic and Behavioral Neurorehabilitation Service from March 2019 to February 2020, were enrolled in this study and randomly divided into two groups: the control group (CG: 53 patients) performed traditional therapy whereas the experimental group (EG:53 patients) received training using semi-immersive VR. All patients underwent the same amount of cognitive training, 3 times a week for 8 weeks. They were submitted to a specific neuropsychological assessment before (T0) and after the rehabilitation treatment (T1).The VR training led to a significant improvement in global cognitive functions, with regard to constructive and ideomotor apraxia. On the contrary, the CG achieved significant improvements only in ideomotor apraxia. Moreover, only in the EG, we observed an improvement in the mood at the end of training.The present study demonstrates that VR rehabilitation can be an effective tool for the treatment of apraxia, which is a neuropsychological problem often underestimated in MS patients. Further studies with long-term follow-up periods are needed to confirm the effect of this promising approach.

Energy efficiency in the building sector is a priority of the EU Commission to achieve carbon neutrality by 2050. Renovation of the existing buildings, which are currently responsible for approximately 40% of EU energy consumption and 36% of the greenhouse gas emissions can lead to significant energy savings. This paper presents the EEnvest calculation method for evaluating the financial impacts of technical risks related to energy-efficient renovation of commercial office buildings. The evaluation method aims to increase investors’ confidence and boost investments in the renovation of the existing building. Through a series of Key Performance Indicators (KPI), the technical and financial risks impact is evaluated. The results are strictly connected to building features, climatic conditions, solution sets and mitigation measures specific to the building energy efficiency project.

Chronic rhinosinusitis (CRS) is a chronic and recurrent disease that negatively affects patients' quality of life. CRS has two main phenotypes: CRS with nasal polyps (CRSwNP) and CRS without polyps (CRSsNP). Minimal research has been conducted to study the variability in patients' characteristics. Therefore, we conducted this study to examine these differences.A retrospective cohort study included patients with both CRSwNP and CRSsNP. Outcomes included symptom severity, radiographic severity, and number of sinus surgeries. Symptom severity was assessed using the Sino-nasal Outcome Test and the Lund-Mackay CT score was used to determine radiographic severity. Further subgroup analysis was done based on the presence or absence of comorbid asthma.A total of 110 and 106 patients were included in the CRSwNP and CRSsNP groups, respectively. The mean age in the CRSwNP and CRSsNP groups was 50.2 and 48.7, and the proportion of female patients was 40.9% and 58.5%, respectively. No significant difference in symptom severity was noted between CRSwNP and CRSsNP group (68.1±18.6 vs. 73.2±21.27; P=0.097), while the Lund-Mackay score was significantly lower in the CRSsNP group (7.4±2.3 vs. 11.9±3.6; P=0.016). Also, the number of surgeries was significantly lower in the CRSsNP group as compared to the CRSwNP group (P=0.023). Subgroup analysis revealed statistically significant differences between those with and without asthma in patients with CRSwNP in terms of Lund-Mackay scores and number of surgeries (P=0.038 and 0.043), respectively. However, no significant differences were noted in the CRSsNP group (P0.05).There is a clear variability in the characteristics of patients with CRSsNP and CRSwNP. A similar difference was noted in the CRSwNP group when patients were stratified based on the presence of absence of asthma. This warrants further investigation of potential correlation with the prognosis and optimum treatment strategies of this patient population.

International audience; We report on a search for nuclear recoil signals from solar $^8$B neutrinos elastically scattering off xenon nuclei in XENON1T data, lowering the energy threshold from 2.6 keV to 1.6 keV. We develop a variety of novel techniques to limit the resulting increase in backgrounds near the threshold. No significant $^8$B neutrino-like excess is found in an exposure of 0.6 t $\times$ y. For the first time, we use the non-detection of solar neutrinos to constrain the light yield from 1-2 keV nuclear recoils in liquid xenon, as well as non-standard neutrino-quark interactions. Finally, we improve upon world-leading constraints on dark matter-nucleus interactions for dark matter masses between 3 GeV/c$^2$ and 11 GeV/c$^2$ by as much as an order of magnitude.

The distribution of floating litter in marine waters, influenced by currents and wind drag, often determines the dispersal of its encrusting fauna. In the present paper, we observed for the first time the colonization of rafting floats from abandoned, lost or derelict fishing gears (ALDFG) by the four protected deep-sea species: Errina aspera, Desmophyllum pertusum, Madrepora oculata Pachylasma giganteum. Overall, 41 floats, colonized by deep benthic species, were found stranded on the shore of the Sicilian coast of the Strait of Messina, between 2016 and 2019. Species composition, number and occurrence of colonizing organisms were analyzed. On the basis of the species composition (the association between E. aspera, P. giganteum and Megabalanus tulipiformis), the knowledge on their ecology, biogeography, path of local currents, it was possible to define that the area of origin of the most part of these fishing net floats was the Strait of Messina.

The Atlantic bluefin tuna (ABFT), Thunnus thynnus (Linnaeus, 1758), is an important commercial species managed as two different stocks, western and eastern Atlantic, with their spawning grounds in the Gulf of Mexico and in the Mediterranean Sea, respectively. The eastern Atlantic stock has been overexploited in the last decades, leading to the application of specific management measures introduced by the International Commission for the Atlantic Tuna (ICCAT). A clear understanding of the genetic structure of ABFT Mediterranean population should be pursued in order to support management decisions. To date the genetic studies on the Mediterranean ABFT, carried out with different molecular markers and sampling procedures, have produced unclear results. Here, we analysed ABFT samples from central and western Mediterranean Sea with mitochondrial sequences and 11 microsatellite loci to investigate, among the others, the area of the Strait of Messina, where environmental conditions seem to support a resident population of ABFT. Furthermore, genetic analyses of mitochondrial sequences were carried out including nucleotide sequences of Adriatic ABFT wild larvae retrieved from GenBank. Among the investigated areas a genetic differentiation was detected between the Strait of Messina and the Tyrrhenian Sea with microsatellite loci according to the exact G test, but not to the Bayesian analyses carried out with STRUCTURE. The analyses with mitochondrial sequences do not reveal any differentiation among sampled areas, however, a highly significant genetic divergence was observed between the Adriatic mitochondrial sequences retrieved from GenBank and the central‐western Mediterranean sequences obtained in the present work. Our results provide some evidence of population structure of Mediterranean ABFT adding pieces to a still unclear picture.

In 2015, the Strait of Sicily, which includes several banks, was candidate as a future Specially Protected Area of Mediterranean Importance (SPAMI) by the Contracting Parties of the Barcelona Convention. In this context, the present study aims to provide the first biological and ecological characterisation of this poorly known area, focusing on habitats and species of conservation concern. Surveys were carried through a remotely operated vehicle (ROV) and allowed the identification of 19 habitat types, mostly considered of priority interest by the SPA/BD Protocol of the Barcelona Convention. A total of 269 taxa were also identified, mostly Porifera, Cnidaria and Pisces, among which 115 species are of conservation concern according to the International Union for Conservation of Nature (IUCN) Red Lists, the Habitats Directive, the Bern Convention, the SPA/BD Protocol and CITES. ROV surveys allowed some interesting observations on poorly known species and habitats. Results underlined that remarkable discrepancies in species conservation status assessment that exist between the several lists of protected species considered. The IUCN Red Lists, although not legally binding for European States, are the most complete, but, in spite of this, the conservation policies in the EU are largely focused on the Habitats Directive, which is really not exhaustive. An exhaustive and legally binding instrument to protect species of conservation concern at European scale is highly recommended. Acquired results could be useful for the delimitation of a future SPAMI or a network of Marine Protected Areas (including the investigated banks) and the identification of zones within them suitable for different area-based management measures.

We report on a search for nuclear recoil signals from solar $^8$B neutrinos elastically scattering off xenon nuclei in XENON1T data, lowering the energy threshold from 2.6 keV to 1.6 keV. We develop a variety of novel techniques to limit the resulting increase in backgrounds near the threshold. No significant $^8$B neutrino-like excess is found in an exposure of 0.6 t $\times$ y. For the first time, we use the non-detection of solar neutrinos to constrain the light yield from 1-2 keV nuclear recoils in liquid xenon, as well as non-standard neutrino-quark interactions. Finally, we improve upon world-leading constraints on dark matter-nucleus interactions for dark matter masses between 3 GeV/c$^2$ and 11 GeV/c$^2$ by as much as an order of magnitude.

This paper presents the findings of a research mission and restoration project, Rupestrian painting in Cappadocia: knowledge, conservation and enhancement, which the University of Tuscia has been conducting in Cappadocia (Turkey) annually since 2006. Carried out in collaboration with our Turkish colleagues from the Archaeological Museum and the Regional Restoration Laboratory of Nevsehir, it is intended to promote knowledge and conservation of the extraordinary wall painting heritage of Cappadocia. Our paper focuses on the materials and techniques of the wall paintings of Cappadocia, based on research carried out over a 13-year period, derived firstly from a survey in the region and then from the experience acquired from conservation projects undertaken at two rock-cut churches, the Church of the Forty Martyrs in Sahinefendi and the New Tokali church in the Goreme Open Air Museum. Findings indicate that the use of different materials and execution techniques can be related to four macro-phases into which we have divided painting practice in Cappadocia. In the overall development of painting up to the eleventh century, there was a general tendency to expand from simplified forms to more complex approaches, which is partly related to the development of more ambitious architectural spaces. In the tenth and eleventh centuries, painting procedures proliferated, which can be associated with the presence of different workshops coexisting in the extended territory between the Open Air Museum and Cavusin. This diversity encompasses a broad range, from the most modest wall painting schemes up to those of an exceptional nature, the latter exemplified by the painting in the New Tokali church or the painting cycles of the so-called ‘Column Churches’.

Home automation (HA) is either a "smart" house or a supportive environment, which enables the patients to regain an active role in daily life. HA could allow people affected by Parkinson Disease (PD) to better manage their daily lives. This study aims to evaluate the effects of domotics on quality of life, and personal/social autonomy in PD patients. We enrolled 40 with PD undergoing neurorehabilitation, who were randomized into either the control (CG) or the experimental group (EG). Two different rehabilitative approaches were used: the CG was submitted to a traditional training, whilst the EG underwent HA training, in which the activities were carried out through the use of assistive technologies. In both the training, the exercises were performed in small samples of 3-5 patients. Results showed that both CG and EG had a significant improvement in global cognitive functioning, executive functions, and instrumental autonomy. However, only in the EG, we observed a significant increase in social adaptation, activities of daily living and quality of life. This pilot study suggests that HA training could be a useful tool for the rehabilitation of patients with PD, improving social and cognitive functioning, personal autonomy and quality of life.

Triple-negative breast cancers (TNBC) comprise biologically and clinically heterogeneous diseases characterized by the lack of hormone receptors (HR) and HER2 expression. This subset of tumors accounts for 15-20% of all breast cancers and pursues an ominous clinical course. However, there is a spectrum of low-risk TNBCs with no/minimal metastatic potential, including the salivary gland-type tumors, those with extensive apocrine differentiation and/or high tumor-infiltrating lymphocytes, and small-sized, early-stage (pT1a/bN0M0) TNBCs. De-escalating the treatment in low-risk TNBC, however, is not trivial because of the substantial lack of dedicated randomized clinical trials and cancer registries. The development of new diagnostic and/or prognostic biomarkers based on clinical and molecular aspects of low-risk TNBCs would lead to improved clinical treatment. Here, we sought to provide a portrait of the clinicopathological and molecular features of low-risk TNBC, with a focus on the diagnostic challenges along with the most important biological characteristics underpinning their favorable clinical course.

Telemedicine may be used for the continuity of care in several chronic conditions. Sixty frail old people were enrolled along with their primary caregivers, and randomly divided into two groups: 30 of them received a multi-specialist telemedicine care, whilst the other 30 were treated in their usual territory care. All of the patients were evaluated through a clinical and psychometric battery at baseline (T0), after 6 months (T1), and at the end of the study (T2). It was found that telemedicine was more effective than the traditional approach in mood improvement (p 0.001), behaviour (p 0.01) and ADL/IADL (p 0.01/0.04), as well as nutritional status. These changes increased over time (from T0 to T1), the caregivers' burden decreased, and system usability was rated as good. Telemedicine could be considered an important tool to improve the psychological health and quality of the life of older frail patients living at home.

Embodied cognition is a theoretical perspective that considers every form of human knowledge and cognition "embodied", as they pass through bodily experience. The aim of this narrative review is to investigate the importance of mirror neurons system in EC, as well as the EC role in neurodegenerative diseases. This narrative review shows deep connections between body and mind: body states influence mental functions such as perception and reasoning, while mental states cause changes in the body, especially in neurodegenerative disorders. Indeed, abnormalities in EC were found in dementia, Parkinson's Disease and Amyotrophic Lateral sclerosis, also in the absence of other cognitive deficits, negatively affecting patients' outcomes. Exploiting EC mechanisms for rehabilitation purposes, also using innovation technologies, could be a promising therapeutic way to increase motor and cognitive outcomes in patients affected by different neurological disorders.

The bluefin tuna, Thunnus thynnus, is a highly migratory and long-living fish at the top of the pelagic food web. As top predator, it plays a key role in the stability of marine food webs by exerting top-down control on its prey. The diet composition of bluefin tuna varies in relation to its growth, seasons and migratory patterns, making it difficult to evaluate spatial and temporal effects. This latter aspect is further complicated to be determined during the first months of life, when T. thynnus specimens have a rapid growth rate leading to changes in the trophic status. In this study, the potential collagen-related effects on δ15N and δ13C values were evaluated on the whole spine of adult tuna specimens collected in the central Mediterranean Sea. Obtained results showed non-significant differences between extracted and non-extracted collagen samples for δ15N in whole spine, allowing adopting the isotopic analysis both for annuli in the spine section of adults and for younger specimens, whose spine size does not permit the collagen extraction. Specifically, isotopic analysis of whole spine of the young of the year specimens, showed a rapid change in δ15N values with length, following an exponential model. For older specimens, δ15N values were higher and varied around a plateau, likely due to a higher specificity in the choice of prey and/or to change in the geographical location. Such variability was also mirrored in annuli of spines sections of adult tunas. As far as δ13C values are concerned, a strong collagen-related effect was evidenced, likely highlighting the influence of lipids. Consequently, δ13C analysis may be used only on adult specimens where collagen extraction is possible. This research also showed how isotopic analysis of both whole sample and sequence of annuli in the cross-section of dorsal spine might produce isotopic profiles useful to detect specific trophic dynamics along the bluefin tuna growth.

The development of Lithium-ion batteries for electric vehicles have recently become the subject of large research efforts due to some remarkable advantages over other kinds of batteries, such as: high energy density, high power density, long cycle life and long calendar life. This paper is aimed to develop an ageing model suitable to estimate the battery lifetime, the residual capacity and reliability margins under different driving cycles, taking also into account the battery calendar ageing.

Background Unmanned Aerial Vehicles (UAVs), a new method of application to deliver pesticides, is rapidly being adopted for commercial use in crop protection in East Asia with increasing worldwide interest. Pest control in mature almond orchards with dense foliar canopies presents greater coverage challenges than field crops and smaller orchard or vineyard crops. We investigated the use of an electric hexacopter to provide acceptable spray deposition and canopy penetration to be considered credible for use in an almond pest control program. Results The performance of the aerial and ground methods at different spray volumes were compared by analyzing spray deposition on water sensitive papers, insecticide residues on filter papers and residues on whole unhulled almonds at three canopy elevations. Overall residue levels of chlorantraniliprole insecticide on whole unhulled almonds across all pooled canopy strata were similar between UAV applied at 46.8 L/ha and 93.5 L/ha and the comparative air blast sprayer treatments applied at 935 L/ha. However, significant interactions between canopy elevation and spray method showed distinct residue patterns between the two application methods. Penetration and spray deposition at the lower canopy were observed and validated for the UAV application. Pest efficacy was evaluated by measuring nut damage at harvest. Conclusion This study presents promising data that support the potential innovative integration of UAV's into crop protection programs for large canopy crops such as almonds and may guide future research for developing relevant label recommendations.

Unmanned Aerial Vehicle (UAV) applications at low-volume using fine and very fine size droplets have been adopted in several commercial spray scenarios allowing water-saving and high-efficiency operation in delivery of pesticides. However, spray drift associated with UAV applications, especially for fine droplets generated from spinning disk nozzles, has not been fully understood, raising environmental and regulatory concerns. The objectives of this study were to compare the drift potential of three different volume median diameter (VMD, or Dv0.5) of 100, 150 and 200 μm from a commercial quadcopter equipped with centrifugal nozzles exposed to different wind speeds under field conditions. Prior to field test, the droplet size of the centrifugal nozzle was measured by a laser-diffraction particle-size analyzer. The results showed that the relationship between rotation speed and Dv0.5 agrees with the negative power function. Field tests found that the deposition at 12 m downwind direction decreased by an order of magnitude compared with the average deposition within the in-swath zone. The deposition of almost all the treatments at 50 m downwind is lower than the detection limits of 0.0002 μL/cm2. Based on the results from this study, the drift distance of this specific very popular UAV model is much less than that of manned aerial applicators. Based on the predicted equation (R2 = 0.83), the detected drift amount increased with increasing wind speed and decreasing Dv0.5. This work provides basic information to quantify the effect of wind speeds and droplet sizes on UAV spray drift potential which supports on-going regulatory guideline development for spray buffer zone and drift risk assessment protocols.