1. miRNA profile is altered in a modified EAE mouse model of multiple sclerosis featuring cortical lesions
- Author
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Rosanna Capasso, Mireille Alhouayek, Giulio G. Muccioli, Owein Guillemot-Legris, Philippe Hantraye, Pauline Bottemanne, Michele Caraglia, Nicola Salvatore Orefice, Giuseppe Orefice, UCL - SSS/LDRI - Louvain Drug Research Institute, Orefice, N. S., Guillemot-Legris, O., Capasso, R., Bottemanne, P., Hantraye, P., Caraglia, M., Orefice, G., Alhouayek, M., and Muccioli, G. G.
- Subjects
0301 basic medicine ,Pathology ,Mouse ,medicine.medical_treatment ,Neuroimmunology ,Autoimmunity ,medicine.disease_cause ,immunology ,Mice ,0302 clinical medicine ,Immunology and Inflammation ,Medicine ,Biology (General) ,Cerebral Cortex ,miR155 ,General Neuroscience ,Experimental autoimmune encephalomyelitis ,autoimmunity ,General Medicine ,Cytokine ,Cytokines ,Female ,medicine.symptom ,Research Article ,medicine.medical_specialty ,Encephalomyelitis, Autoimmune, Experimental ,QH301-705.5 ,Science ,Immunology ,Inflammation ,neuroimmunology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,microRNA ,Animals ,General Immunology and Microbiology ,business.industry ,Animal ,Multiple sclerosis ,medicine.disease ,Mice, Inbred C57BL ,MicroRNAs ,030104 developmental biology ,inflammation ,business ,Transcriptome ,Human Pathology ,030217 neurology & neurosurgery ,Neuroscience - Abstract
Cortical lesions represent a hallmark of multiple sclerosis and are proposed as a predictor of disease severity. microRNAs are suggested to be important players in the disease pathogenesis and the experimental autoimmune encephalomyelitis animal model. We implemented a mouse model recapitulating more closely the human pathology as it is characterized by both an autoimmune heterogeneity and the presence of cortical lesions, two parameters missing in experimental autoimmune encephalomyelitis. In our model, mice clustered in two groups displaying high or low clinical scores. Upon cortical cytokine injection, lesions appeared with a specific topography while cortical miRNA profiles were altered. These two features differed according to disease severity. We evidenced changes in miRNA regulators and targets suggesting that miRNA alteration had functional repercussions that could explain the differences in cortical lesions. This model represents a crucial tool for the study of both miRNA involvement and cortical lesion formation in disease pathogenesis.
- Published
- 2020