9 results on '"Alexandra Cervantes"'
Search Results
2. HACER AGUA BEBIBLE
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Johanna Alexandra Cervantes García
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- 2019
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3. Clinical Outcomes and Sustainability of Using CYP2C19 Genotype–Guided Antiplatelet Therapy After Percutaneous Coronary Intervention
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Melissa J. Polasek, Shivanshu Madan, Kasey Hamrick, Vindhya B. Sriramoju, Jonathan D. Cicci, Megan Clarke, Lucius A. Howell, Craig R. Lee, Alexandra Cervantes, Nicholas Varunok, George A. Stouffer, Karen E. Weck, and John Andrew Lee
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medicine.medical_specialty ,Acute coronary syndrome ,animal structures ,Ticlopidine ,Prasugrel ,Genotype ,medicine.medical_treatment ,CYP2C19 ,030204 cardiovascular system & hematology ,Article ,03 medical and health sciences ,Percutaneous Coronary Intervention ,0302 clinical medicine ,Internal medicine ,medicine ,030212 general & internal medicine ,business.industry ,Hazard ratio ,Percutaneous coronary intervention ,General Medicine ,medicine.disease ,Clopidogrel ,Cytochrome P-450 CYP2C19 ,business ,Ticagrelor ,Platelet Aggregation Inhibitors ,Pharmacogenetics ,medicine.drug - Abstract
Background: CYP2C19 loss-of-function (LOF) alleles impair clopidogrel effectiveness after percutaneous coronary intervention. The feasibility, sustainability, and clinical impact of using CYP2C19 genotype–guided dual antiplatelet therapy (DAPT) selection in practice remains unclear. Methods: A single-center observational study was conducted in 1193 patients who underwent percutaneous coronary intervention and received DAPT after implementation of an algorithm that recommends CYP2C19 testing in high-risk patients and alternative DAPT (prasugrel or ticagrelor) in LOF allele carriers. The frequency of genotype testing and alternative DAPT selection were the primary implementation end points. Risk of major adverse cardiovascular or cerebrovascular and clinically significant bleeding events over 12 months were compared across genotype and DAPT groups by proportional hazards regression. RESULTS: CYP2C19 genotype was obtained in 868 (72.8%) patients. Alternative DAPT was prescribed in 186 (70.7%) LOF allele carriers. CYP2C19 testing ( P P =0.001) varied over time. Risk for major adverse cardiovascular or cerebrovascular was significantly higher in LOF carriers prescribed clopidogrel versus alternative DAPT (adjusted hazard ratio, 4.65; 95% confidence interval, 2.22–10.0; P P =0.347). Bleeding event rates were similar across groups (log-rank P =0.816). Conclusions: Implementing CYP2C19 genotype–guided DAPT is feasible and sustainable in a real-world setting but challenging to maintain at a consistently high level of fidelity. The higher risk of major adverse cardiovascular or cerebrovascular associated with clopidogrel use in CYP2C19 LOF allele carriers suggests that use of genotype-guided DAPT in practice may improve clinical outcomes.
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- 2018
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4. [Untitled]
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Alexandra Cervantes, Daniel Cardin, and Lindsay Daniels
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medicine.medical_specialty ,business.industry ,medicine.drug_class ,Intensive care ,Antibiotics ,Ventilator-associated pneumonia ,Medicine ,Critical Care and Intensive Care Medicine ,business ,Intensive care medicine ,medicine.disease - Published
- 2019
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5. THIRTY DAY CLINICAL OUTCOMES FOLLOWING IMPLEMENTATION OF CYP2C19 GENOTYPE-GUIDED DUAL ANTIPLATELET THERAPY
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Nicholas Varunok, George A. Stouffer, Alexandra Cervantes, Vindhya B. Sriramoju, Craig R. Lee, Melissa D. Klein, Shivanshu Madan, and Karen E. Weck
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medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Cyp2c19 genotype ,Percutaneous coronary intervention ,Retrospective cohort study ,CYP2C19 ,Single Center ,surgical procedures, operative ,Internal medicine ,THIRTY-DAY ,Conventional PCI ,medicine ,cardiovascular diseases ,Cardiology and Cardiovascular Medicine ,business ,Genotyping - Abstract
The clinical impact of using CYP2C19 genotype to guide dual antiplatelet therapy (DAPT) after percutaneous coronary intervention (PCI) remains unclear. This single center retrospective cohort study included 1193 PCI patients from 2012-14. CYP2C19 genotyping was recommended in high risk patients
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- 2018
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6. Melanin-concentrating hormone induces neurite outgrowth in human neuroblastoma SH-SY5Y cells through p53 and MAPKinase signaling pathways
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Jean-Louis Nahon, Natacha Cotta-Grand, Alexandra Cervantes, Frédéric Brau, Alice Guyon, Carole Rovère, Maisonneuve-Rosemont Hospital Research Center, University of Montreal, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), and Association pour la Recherche sur le Cancer, Centre National de la Recherche Scientifique 2004), European Community, ANR-MNP 2008
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p53 ,Patch-Clamp Techniques ,SH-SY5Y ,Melanin-concentrating hormone ,Physiology ,Biochemistry ,Neuroblastoma ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Phosphorylation ,Receptor ,Mitogen-Activated Protein Kinase 1 ,0303 health sciences ,Hypothalamic Hormones ,Mitogen-Activated Protein Kinase 3 ,Reverse Transcriptase Polymerase Chain Reaction ,Neurite outgrowth ,respiratory system ,MCH-R1 ,Immunohistochemistry ,Cell biology ,Blotting, Southern ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Signal transduction ,hormones, hormone substitutes, and hormone antagonists ,Signal Transduction ,medicine.medical_specialty ,Neurite ,Blotting, Western ,MAPKinase ,Biology ,Cell Line ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Cell Line, Tumor ,Internal medicine ,Neurites ,medicine ,Humans ,Receptors, Pituitary Hormone ,Transcription factor ,Early Growth Response Protein 1 ,ets-Domain Protein Elk-1 ,030304 developmental biology ,Flavonoids ,Melanins ,MCH ,Pituitary Hormones ,chemistry ,Cell culture ,Tumor Suppressor Protein p53 ,030217 neurology & neurosurgery - Abstract
International audience; Melanin-concentrating hormone (MCH) peptide plays a major role in energy homeostasis regulation. Little is known about cellular functions engaged by endogenous MCH receptor (MCH-R1). Here, MCH-R1 mRNA and cognate protein were found expressed in human neuroblastoma SH-SY5Y cells. Electrophysiological experiments demonstrated that MCH modulated K(+) currents, an effect depending upon the time of cellular growth. MCH treatments induced a transient phosphorylation of MAPKinases, abolished by PD98059, and partially blocked by PTX, suggesting a Galphai/Galphao protein contribution. MCH stimulated expression and likely nuclear localization of phosphorylated p53 proteins, an effect fully dependent upon MAPKinase activities. MCH treatment also increased phosphorylation of Elk-1 and up-regulated Egr-1, two transcriptional factors targeted by the MAPKinase pathway. Finally, MCH provoked neurite outgrowth after 24h-treatment of neuroblastoma cells. This effect and transcriptional factors activation were partly prevented by PD98059. Collectively, our results provide the first evidence for a role of MCH in neuronal differentiation of endogenously MCH-R1-expressing cells via non-exclusive MAPKinase and p53 signaling pathways.
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- 2009
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7. Phosphorylation of the atypical kinesin Costal2 by the kinase Fused induces the partial disassembly of the Smoothened-Fused-Costal2-Cubitus interruptus complex in Hedgehog signalling
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Arnaud Truchi, Laurent Ruel, Sophie Raisin, Alexandra Cervantes, Pascal P. Thérond, Laurence Staccini-Lavenant, Armel Gallet, Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)
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MESH: Signal Transduction ,Embryo, Nonmammalian ,Kinesins ,MESH: Receptors, G-Protein-Coupled ,Receptors, G-Protein-Coupled ,Serine ,0302 clinical medicine ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,Drosophila Proteins ,MESH: Animals ,Phosphorylation ,0303 health sciences ,Kinase ,MESH: Transcription Factors ,Smoothened Receptor ,Transmembrane protein ,Cell biology ,DNA-Binding Proteins ,Drosophila melanogaster ,Phenotype ,Biochemistry ,Kinesin ,RNA Interference ,Signal transduction ,Signal Transduction ,animal structures ,MESH: Drosophila Proteins ,MESH: RNA Interference ,Protein Serine-Threonine Kinases ,Biology ,MESH: Phenotype ,MESH: Protein-Serine-Threonine Kinases ,MESH: Drosophila melanogaster ,Cell Line ,03 medical and health sciences ,MESH: Homeodomain Proteins ,Animals ,Hedgehog Proteins ,MESH: Serine ,Molecular Biology ,Hedgehog ,030304 developmental biology ,Homeodomain Proteins ,Binding Sites ,MESH: Phosphorylation ,MESH: Embryo, Nonmammalian ,MESH: Hedgehog Proteins ,MESH: Multiprotein Complexes ,MESH: Cell Line ,MESH: Binding Sites ,Multiprotein Complexes ,MESH: Kinesin ,Smoothened ,MESH: DNA-Binding Proteins ,030217 neurology & neurosurgery ,Transcription Factors ,Developmental Biology - Abstract
The Hedgehog (Hh) family of secreted proteins is involved both in developmental and tumorigenic processes. Although many members of this important pathway are known, the mechanism of Hh signal transduction is still poorly understood. In this study, we analyse the regulation of the kinesin-like protein Costal2 (Cos2) by Hh. We show that a residue on Cos2,serine 572 (Ser572), is necessary for normal transduction of the Hh signal from the transmembrane protein Smoothened (Smo) to the transcriptional mediator Cubitus interruptus (Ci). This residue is located in the serine/threonine kinase Fused (Fu)-binding domain and is phosphorylated as a consequence of Fu activation. Although Ser572 does not overlap with known Smo-or Ci-binding domains, the expression of a Cos2 variant mimicking constitutive phosphorylation and the use of a specific antibody to phosphorylated Ser572 showed a reduction in the association of phosphorylated Cos2 with Smo and Ci,both in vitro and in vivo. Moreover, Cos2 proteins with an Ala or Asp substitution of Ser572 were impaired in their regulation of Ci activity. We propose that, after activation of Smo, the Fu kinase induces a conformational change in Cos2 that allows the disassembly of the Smo-Fu-Cos2-Ci complex and consequent activation of Hh target genes. This study provides new insight into the mechanistic regulation of the protein complex that mediates Hh signalling and a unique antibody tool for directly monitoring Hh receptor activity in all activated cells.
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- 2007
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8. A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila
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Sandra Lacas-Gervais, Laurent Ruel, Alexandra Cervantes, Sébastien Schaub, Gisela D'Angelo, Reid Aikin, Pascal P. Thérond, D'ANGELO, Gisela, Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Centre Commun de Microscopie Appliquée (CCMA), Université de Nice Sophia-Antipolis (UNSA), Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)
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Genome, Insect ,Intracellular Space ,lcsh:Medicine ,Gene Expression ,Golgi Apparatus ,Genes, Insect ,MESH: Genes, Insect ,Animals, Genetically Modified ,MESH: Cholesterol ,0302 clinical medicine ,MESH: Luciferases, Renilla ,Molecular Cell Biology ,Drosophila Proteins ,MESH: Animals ,lcsh:Science ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,COPII ,0303 health sciences ,Multidisciplinary ,MESH: Genetic Testing ,Genomics ,Animal Models ,COPI ,MESH: Wnt1 Protein ,Cell biology ,Transport protein ,MESH: Reproducibility of Results ,Protein Transport ,Cholesterol ,Drosophila melanogaster ,MESH: Intracellular Space ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,RNA Interference ,Drosophila Protein ,Research Article ,Subcellular Fractions ,Morphogen ,MESH: Protein Transport ,MESH: Drosophila Proteins ,Recombinant Fusion Proteins ,MESH: RNA Interference ,Wnt1 Protein ,Biology ,MESH: Drosophila melanogaster ,MESH: Animals, Genetically Modified ,MESH: Golgi Apparatus ,03 medical and health sciences ,Model Organisms ,MESH: RNA, Double-Stranded ,Genome Analysis Tools ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,MESH: Recombinant Fusion Proteins ,Genetics ,Animals ,Hedgehog Proteins ,Secretion ,Genetic Testing ,Hedgehog ,Secretory pathway ,Luciferases, Renilla ,RNA, Double-Stranded ,030304 developmental biology ,MESH: Genome, Insect ,lcsh:R ,Reproducibility of Results ,MESH: Hedgehog Proteins ,Molecular Development ,MESH: Protein Processing, Post-Translational ,MESH: Subcellular Fractions ,lcsh:Q ,Organism Development ,Protein Processing, Post-Translational ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
International audience; Hedgehog (Hh) proteins are secreted molecules that function as organizers in animal development. In addition to being palmitoylated, Hh is the only metazoan protein known to possess a covalently-linked cholesterol moiety. The absence of either modification severely disrupts the organization of numerous tissues during development. It is currently not known how lipid-modified Hh is secreted and released from producing cells. We have performed a genome-wide RNAi screen in Drosophila melanogaster cells to identify regulators of Hh secretion. We found that cholesterol-modified Hh secretion is strongly dependent on coat protein complex I (COPI) but not COPII vesicles, suggesting that cholesterol modification alters the movement of Hh through the early secretory pathway. We provide evidence that both proteolysis and cholesterol modification are necessary for the efficient trafficking of Hh through the ER and Golgi. Finally, we identified several putative regulators of protein secretion and demonstrate a role for some of these genes in Hh and Wingless (Wg) morphogen secretion in vivo. These data open new perspectives for studying how morphogen secretion is regulated, as well as provide insight into regulation of lipid-modified protein secretion.
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- 2012
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9. Studying the role of sterols in Hedgehog signaling
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Armel Gallet, Gisela D'Angelo, Riikka Hynynen, Alexandra Cervantes, Laurent Ruel, Aline Scholler Joulié, and Pascal P. Thérond
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Chemistry ,Organic Chemistry ,Cell Biology ,Molecular Biology ,Biochemistry ,Hedgehog signaling pathway ,Cell biology - Published
- 2011
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