1. Experimental study of the quantification of indocyanine green fluorescence in ischemic and non-ischemic anastomoses, using the SERGREEN software program
- Author
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Borja Serra-Gomez, Anna Pallisera-Lloveras, Xavier Serra-Aracil, Albert Garcia-Nalda, Laura Mora-López, Victoria Lucas-Guerrero, Salvador Navarro-Soto, and Alvaro Serra-Gomez
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Indocyanine Green ,Multidisciplinary ,genetic structures ,Swine ,business.industry ,Anastomosis, Surgical ,Anastomotic Leak ,Anastomosis ,Fluorescence ,Ischemia ,Animals ,Medicine ,Non ischemic ,business ,Nuclear medicine ,Software ,Indocyanine green fluorescence - Abstract
Background: Tissue ischemia is a key risk factor for anastomotic leakage (AL). Indocyanine green (ICG) is widely used in colorectal surgery to define the segments with the best vascularization. In an experimental model, we present a new system for quantifying ICG saturation, SERGREEN software.Methods: This was a controlled experimental study with eight pigs. In the initial control stage, ICG saturation was analyzed at the level of two anastomoses in the right and left colon. Control images of the two segments were taken after ICG administration. The images were processed with the SERGREEN program. Then, in the experimental ischemia stage, the inferior mesenteric artery was sectioned at the level of the anastomosis of the left colon. Fifteen minutes after the section, sequential images of the two anastomoses were taken every 30’ for the following 2 h.Results: At the control stage, the mean scores were 134.2 (95% CI: 116.3-152.2) for the right colon and 147 (95% CI: 134.7-159.3) for the left colon (p = 0.174). The right colon remained stable throughout the experiment. In the left colon, saturation fell by 47.9 points with respect to the preischemia value (p Conclusions: The SERGREEN program quantifies ICG saturation in normal and ischemic situations and detects differences between them. A reduction in ICG saturation of 32.6% or more was correlated with complete tissue ischemia.
- Published
- 2022
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