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Background: MicroRNAs (miRNA/miR) play significant roles in the regulation of cell differentiation, cell cycle progression, and apoptosis. They become dysregulated during carcinogenesis and are eventually released into the circulation, enabling their detection in body fluids. Thus, this study compared the miRNA expression in tissue and plasma samples of colorectal cancer (CRC) patients and clinically healthy controls and determined miRNA expression as a potential CRC biomarker.Methods: Using quantitative reverse transcription polymerase chain reaction (RT-qPCR), miR-21-5p, miR-29a-3p, miR-92a-3p, miR-135b-5p, miR-196b-5p, and miR-197-3p, expression was analyzed and compared between the malignant (n = 41) and the adjacent neoplasm free mucosal tissues (n = 41) of CRC patients. The findings were validated in plasma samples (n = 36) collected from the same CRC patients prior to surgery or any form of treatment and compared to plasma from their age and sex-matched controls (n = 36).Results: MiR-21-5p, miR-29a-3p, miR-92a-3p, and miR- 196b-5p were upregulated and miR-135b-5p was downregulated in CRC malignant tissues compared to their expression in adjacent neoplasm-free tissue. This was further observed in the plasma of the same CRC cases compared to controls. MiR-92a-3p showed itself the most sensitive (0.93; p < .001) and most specific (0.95; p < .001) in detecting CRC in tissue. In plasma, miR-196b-5p was the most sensitive (0.97; p < .001) and specific (0.94; p < .001) in detecting CRC. Plasma miR-92a-3p and miR-196b-5p were the most sensitive (0.95; p < .001) and specific (0.94; p < .001) in the early detection of CRC.Conclusions: Results show that specific miRNAs dysregulated in malignanttissues are released and can be detected in the circulation, supporting their potential as non-invasive biomarkers of CRC.

The present study analyzed trends in premature mortality from Noncommunicable diseases (NCDs) between 1990 and 2019, the projections up to 2030, and the risk factors (RFs) attributable to these diseases in the Community of Portuguese Language Countries (CPLP). Estimates from the Global Burden of Disease (GBD) study and the analysis of the burden of premature mortality due to NCDs were used for nine CPLP countries, applying age-standardized rates, using RStudio. Portugal, Brazil, Equatorial Guinea, Angola, and Guinea Bissau showed declining premature mortality rates caused by NCDs, while East Timor, Cape Verde, São Tomé and Príncipe, and Mozambique showed an increase in rates. Projections indicate that none of the countries is expected to achieve the goals of reducing premature mortality due to NCDs by one third by 2030. The attributable burden of disease showed that the most important RFs in 2019 were: high systolic blood pressure (SBP), tobacco, dietary risks, high body mass index (BMI), and air pollution. It can therefore be concluded that there are profound differences in the burden of NCDs among the countries, with better results in Portugal and Brazil, and that no CPLP country is likely to reach the NCD reduction target by 2030.

Um dos grandes e atuais desafios para o desenvolvimento brasileiro é manter o crescimento da produção no meio rural e, ao mesmo tempo, reduzir os impactos dessa produção sobre os recursos naturais. Esse desafio surge em meio aos debates internacionais e nacionais em resposta às pressões cada vez maiores da sociedade por um novo modelo de desenvolvimento, que seja capaz de conciliar a conservação do meio ambiente e ao desenvolvimento social e econômico da Amazônia. As florestas de bambu dominam as paisagens do Acre ocupando as tipologias florestais que ocorrem em 62% do território acreano. Neste contexto, esta pesquisa tem como objetivo estimar o volume de bambu de espécies do gênero Guadua no estado do Acre, Brasil. Em campo foi obtido através de inventários florestais o número de hastes e o volume de bambu em áreas de ocorrência das espécies por tipologia florestal: Guadua angustifolia, Guadua sacocarpa e Guadua weberbaueri. Os resultados revelam a ocorrência estimada de 21,8 bilhões de hastes, equivalentes a um volume total de 800,1 milhões de m³ de bambu Guadua spp. Desta forma, o Acre apresenta um grande potencial de fornecer matéria prima para a indústria moveleira, construção civil e para outros segmentos produtivos, à base de bambu, que é atualmente considerado por muitos autores, “a matéria prima do futuro”.

Here, we report real-world evidence on the safety and efficacy of nilotinib as a first-line treatment in elderly patients with chronic phase CML, treated in 18 Italian centers. Sixty patients aged > 65 years (median age 72 years (65–84)) were reported: 13 patients were older than 75 years. Comorbidities were recorded at baseline in 56/60 patients. At 3 months of treatment, all patients obtained complete hematological response (CHR), 43 (71.6%) an early molecular response (EMR), while 47 (78%) reached a complete cytogenetic response (CCyR). At last follow-up, 63.4% of patients still had a deep molecular response (MR4 or better), 21.6% reached MR3 as best response and 11.6% persisted without MR. Most patients (85%) started the treatment at the standard dose (300 mg BID), maintained at 3 months in 80% of patients and at 6 months in 89% of them. At the last median follow-up of 46.3 months, 15 patients discontinued definitively the treatment (8 due to side effects, 4 died for unrelated CML causes, 1 for failure, 2 were lost to follow-up). One patient entered in treatment-free remission. As to safety, 6 patients (10%) experienced cardiovascular events after a median time of 20.9 months from the start. Our data showed that nilotinib could be, as first-line treatment, effective and relatively safe even in elderly CML patients. In this setting, more data in the long term are needed about possible dose reduction to improve the tolerability, while maintaining the optimal molecular response.

Anatomical variants of the long head of biceps (LHB) tendon are widely discussed in the literature. As one of the few intra-articular tendons, magnetic resonance arthroscopy can quickly evaluate the proximal part of LHB morphology. It provides good assessment of both intra-articular and extra-articular portions of the tendons. In-depth knowledge about imaging of the anatomical LHB variants discussed in this article is useful preoperatively for orthopaedists and also helps avoid potential diagnostic misinterpretations.

Purpose. We present a rare case of Citrobacter koseri culture-positive endophthalmitis in a postvitrectomy silicone oil-filled eye. Case report. A 64-year-old male patient presented to our ophthalmology emergency room with representative symptoms of acute endophthalmitis. He underwent a plana vitrectomy oil-filled tamponade previously. Preoperative and postoperative findings of the case were reported. Results. Culture tests of aqueous, silicone oil, and vitreous taps were positive for Citrobacter koseri. Conclusions. Culture-positive endophthalmitis in a silicone oil-filled eye has very rarely been in the literature. The described cases were caused by acute inflammatory reactions to silicone oil and were culture-negative. The postvitrectomy culture-positive endophthalmitis caused by Citrobacter is a very rare condition, and its management is not so smooth. Approaching with silicone oil removal, intraoperative intravitreal antibiotic injection, and silicone oil reinjection was performed in our case with good outcomes.

Producción Científica, A preference–approval on a set of alternatives consists of a weak order on that set and, additionally, a cut-off line that separates acceptable and unacceptable alternatives. In this paper, we propose a new method for defining the distance between preference–approvals taking into account jointly the disagreements in preferences and approvals for each pair of alternatives. The proposed distance is compared to the existing distance functions to deal with clustering problems. Specifically, we prove that our metric improves the estimated clusters in terms of both stability and accuracy., Agencia Estatal de Investigación (Project PID2021-122506NB-I00), University of Palermo (Projects: FFR_D16_PLAIA and FFR_D16_SCIANDRA)

Several studies have shown that ultrasound guidance may contribute to improved safety, effectiveness and accuracy of musculoskeletal interventional procedures performed around the hip if compared to those performed with a landmark-guided technique. Different approaches and injectates can be used for treating hip musculoskeletal disorders. These procedures may involve injections in the hip joint, periarticular bursae, tendons, and peripheral nerves. Intra-articular hip injections are mostly used as a conservative approach for treating patients affected by hip osteoarthritis. Ultrasound-guided injection of the iliopsoas bursa is performed in patients with bursitis and/or tendinopathy, to treat those with painful prosthesis due to iliopsoas impingement, or when the lidocaine test is indicated to identify the iliopsoas as a source of pain. Ultrasound-guided interventions are routinely used in patients with greater trochanteric pain syndrome having as target the gluteus medius/minimus tendons and/or the trochanteric bursae. Ultrasound-guided fenestration and platelet-rich plasma injection are applied in patients with hamstring tendinopathy with good clinical outcomes. Last but not least, ultrasound-guided perineural injections can be used for peripheral neuropathies or blocks of the sciatic, lateral femoral cutaneous, and pudendal nerves. In this paper, we discuss the evidence and technical tips for musculoskeletal interventional procedures performer around the hip, highlighting the added value of ultrasound as an imaging guidance modality.

O presente artigo tem como objetivo, refletir sobre as transformações que ocorreram na instituição jornalística ao longo dos anos e que resultaram no desvelamento de suas práticas, destacando o processo de reconfiguração vivenciado pelo jornalismo nos dias atuais, onde novas habilidades são necessárias para lidar com o avanço da internet e, consequentemente, das redes sociais. Nesse cenário digital, o jornalismo se depara com a desinformação que se alastra pelo mundo a passos largos e coloca em xeque sua credibilidade. Como alternativa, o fact checking que, através do trabalho de verificação dos fatos, auxilia o jornalismo no combate à desinformação. Este texto se estrutura em uma abordagem qualitativa exploratória sobre o tema em questão e apresenta reflexões acerca das reconfigurações das práticas jornalísticas.

The anomalous behaviour of Eu3+ emission quenching on cooling is explained using experimental/theoretical approaches involving off-resonance excitation. Based on this, a highly sensitive CaTiO3:Eu3+,Er3+ phosphor (Sr = 4.9% K−1 at 83 K) was designed.

A new ligation method for piling up helical aromatic foldamers into rigid coaxial stacks has allowed the formation of discrete dimers and of dynamic supramolecular polymers.

Este estudo describe a influencia que exerce o francés L2 na aprendizaxe de italiano L4 por parte de estudantes adultos de fala turca nun contexto universitario. O noso estudo céntrase, concretamente, na interpretación e tradución de trece colocacións italianas do tipo «fare + substantivo» e «fare + determinante + substantivo». Describimos e analizamos as decisións tradutolóxicas destas colocacións baseándonos na lingüística analóxica (Hofstadter 1995; Gentner et al., 2001; Monneret, 2011 e 2018) e na semántica cognitiva (Lakoff e Johnson, 1980 e 1999). O noso propósito é destacar a importancia de empregar unha lingua franca, coma o francés, para fomentar a aprendizaxe das construcións fraseolóxicas italianas ante un público de fala turca.

RE-PERG can detect variations in the metabolic status of the RGCs in glaucomatous patients after oral intake of a fixed combination of C+H in addition to topical treatment. RE-PERG can be a valid tool in neuroenhancement studies, representing a biological marker of neuronal performance

The inverse-scattering imaging condition (ISIC) for reverse time migration (RTM) aims at recovering amplitudes proportional to seismic reflectivity. It has been derived as the high-frequency asymptotic inverse of Born modeling, which justifies its being called a true-amplitude imaging condition. It involves the temporal and spatial derivatives of the up- and downgoing wavefields, in this way generalizing the conventional crosscorrelation imaging condition. The temporal derivations can be redistributed between different wavefield contributions, in this way deriving a set of different implementational forms of the ISIC. By making use of the wave equation for the up- and downgoing wavefields, one can substitute the time derivatives by the Laplacian operator. This provides a theoretical foundation for a popular filter for reducing the backscattering artifacts in RTM. Using Born data from a simple three-layer model and the Marmousi II model as well as the Sigsbee2b data, we have determined that the theoretical equivalence of the equations leads to similar but not identical images. Our numerical tests indicate that the ISIC versions using spatial derivatives are the most economical approach, and that the images obtained with the second time derivative of the source wavefield indicate slightly improved resolution over the other implementations, making the combination of these two characteristics the best choice.

Objetivo: relatar um caso de linfangiomatose difusa. Métodos: relatamos o caso de um paciente masculino, 27 anos, com derrames cavitários de repetição e diagnóstico de linfangiomatose disseminada sugerido por exames de imagem, com acometimento torácico, esplênico, vesical e ósseo. Submetido ao tratamento com propranolol, alendronato, talidomida e bevacizumabe. Resultados: apesar do tratamento instituído, não houve resposta clínica e radiológica satisfatória, evoluindo com coagulação intravascular disseminada (CIVD) e óbito. Conclusão: A linfangiomatose difusa é uma doença de prognóstico ruim na maioria dos casos. A CIVD no caso relatado pode estar associada a complicações da doença.

O presente estudo objetivou analisar as concepções de educadores de creches públicas acerca do desenvolvimento da criança na faixa etária de 24 a 35 meses de idade, considerando, a partir da perspectiva do desenvolvimento de Vygotsky, que o processo de constituição das concepções apresenta um caráter dinâmico na medida em que são influenciadas pelo contexto social, histórico e cultural do indivíduo. Foram realizadas entrevistas semiestruturadas com três educadoras de creches públicas da cidade de João Pessoa-PB. Os dados foram analisados a partir da técnica de análise de conteúdo temática. Os resultados revelaram que as concepções das educadoras sobre o desenvolvimento infantil são demonstradas a partir da caracterização que estas atribuem a esse processo, destacando que a etapa da educação infantil tem expressiva influência no desenvolvimento da criança, sobretudo nas seguintes dimensões: a educação infantil como base para etapas posteriores do desenvolvimento; a perspectiva do desenvolvimento infantil enquanto processo; e a aprendizagem da criança compreendida numa perspectiva para além do brincar e do cuidar. Tais concepções são expressas ainda pelos aspectos que estas consideram significativos no desenvolvimento da criança de 24-35 meses tais como: cognição, linguagem, interação social, motricidade e autonomia.

A violência sexual infantil reveste-se de singular importância em virtude das consequências para o crescimento e desenvolvimento da criança e do adolescente, além da possibilidade de construção da matriz de reprodução da violência para gerações futuras, a partir da aprendizagem quanto às atitudes violentas. O objetivo do estudo foi analisar o reconhecimento e o manejo clínico das crianças e adolescentes vítimas de violência sexual (VS) na Atenção Primária à Saúde (APS). A metodologia empregada foi a revisão integrativa da literatura, tendo a busca sido efetuada na Scientific Electronic Library Online (SciELO) e na Biblioteca Virtual em Saúde (BVS). Utilizaram-se os descritores, em português e inglês, devidamente indexados conforme os Descritores em Ciências da Saúde: abuso sexual na infância; atenção primária à saúde; childhood sexual abuse; primary health care. Os critérios de inclusão foram: (1) artigos originais; (2) artigos de revisão; (3) artigos redigidos em língua portuguesa ou inglesa; (4) e que disponibilizavam o resumo nas bases de dados. A busca inicial resultou em 123 estudos. Excluídos os que não preencheram os critérios, os que não respondiam à pergunta da pesquisa e as duplicidades, resultaram oito artigos. Poucos estudos clínicos foram encontrados quando comparados aos ensaios teóricos e às revisões de literatura. Os achados recomendam a obtenção de uma história clínica, realização de exame físico e aquisição de exames laboratoriais apropriados. Além disso, são essenciais: determinar a necessidade de reportar a suspeita do abuso; avaliar as consequências físicas, emocionais e comportamentais do VS; fornecer informação aos pais sobre como apoiar seus filhos; e, dependendo de cada caso, encaminhar os pacientes a outros profissionais. Constata-se, portanto, a importância dos médicos e profissionais de saúde familiarizar-se com o tema, podendo, assim, reconhecer precocemente os casos suspeitos e, então realizar o acompanhamento longitudinal das vítimas de abuso sexual infantil.

Trata-se de uma análise epidemiológica sobre as notificações de síndrome gripal (SG) com o SARS-CoV-2 como agente etiológico em uma cidade do sertão da Paraíba, tomando base o início das notificações no e-SUS Notifica, em março de 2020, até a última semana epidemiológica de 2021. Tem por objetivo analisar o perfil dos casos de COVID-19, registrados, o que ajuda a entender o comportamento da pandemia ao longo do tempo, bem como observar a efetividade da vacinação. A partir desta pesquisa, constatou-se que a cidade de Pombal-PB teve 5.023 notificações de casos de SG, mas apenas 2.201 se enquadraram nos critérios para análise. Destes, 46,5% eram do sexo masculino e 53,5% do sexo feminino. As informações coletadas também demostraram uma discrepância entre esses dados e os disponíveis pelo governo do Estado da Paraíba, tanto pelo fato do Estado ter acesso a outros sistemas de coleta de informações, como por faltas durante os registros no sistema. Além disso, observou-se que as manifestações clínicas mais descritas foram dor de garganta (35,8%, N=853), tosse (34,9%, N= 768) e febre (34,3%, N= 754), o que divergiu da literatura encontrada. Entretanto, este estudo ainda é falho devido à subnotificação dos casos, de forma que não se tem como afirmar a confiabilidade da informação. Dessa forma, sugerimos que estratégias amplas sejam implementadas para fechamento dos casos em aberto no sistema, além de capacitação dos profissionais responsáveis pela digitalização de tais registros, para que, no futuro, tenhamos dados públicos mais fidedignos com a realidade.

O presente estudo aborda uma ramificação da área da logística, a logística reversa, que consiste em ações voltadas para a reutilização de resíduos, reaproveitamento de qualquer descarte ou sobras da produção, descarte correto de lixo, bem como ações de conscientização ambiental dos colaboradores e consumidores. Ante ao exposto, o presente artigo, visa investigar: como a logística reversa pode ser aplicada em empresas do ramo alimentício? Sendo assim, tem-se como objetivo apresentar a logística reversa como um diferencial competitivo para as organizações que atuam com alimentos. Para isso, a metodologia utilizada foi bibliográfica, sendo realizada uma pesquisa em livros, sites e artigos científicos. Como resultados, verificou-se que, devido à crescente preocupação com a preservação do meio ambiente, a logística reversa vem sendo cada vez mais estudada, a fim de diminuir os danos significativos e irreversíveis que o descarte incorreto de lixo acarreta ao planeta. Por fim, concluiu-se que a logística reversa, quando aplicada em empresas do ramo alimentício, pode-se apresentar como um diferencial competitivo, uma vez que agrega uma boa imagem à empresa e fideliza os clientes pelo fluxo reverso, que se dá pelo fato de as empresas oferecem um suporte no pós-venda ou pós-consumo do produto.

Minimally invasive mitral valve surgery leads to shorter postoperative recovery time, cosmetic advantages and significant pain reduction compared with the standard sternotomy approach. Both an external aortic clamp and an endoaortic balloon occlusion can be used to manage the ascending aorta and the myocardial protection. In this study, we aimed to compare these two strategies in terms of effectiveness of myocardial protection and associated early postoperative outcomes.We investigated the retrospective records of prospectively collected data of patients treated by minimally invasive mitral valve surgery from March 2014 to June 2019. A total of 180 cases (78 in the external aortic clamp group and 102 in the endoaortic balloon clamp group) were collected. A propensity weighting analysis was adopted to adjust for baseline variables.The endoaortic balloon clamp presented higher EuroSCORE II (higher reoperative surgery rate). The intra- and postoperative data were similar between the two groups: the postoperative troponin-I levels, peak of serum lactates and rate of myocardial infarction were also comparable. The endoaortic clamp group recorded longer operative, cardiopulmonary bypass and cross-clamp times. The external clamp group showed a higher rate of postoperative atrial fibrillation and conduction block.In experienced centers, the use of the endoaortic balloon clamp is safe, reproducible and comparable to the external aortic clamp regarding the effectiveness of myocardial protection: its employment might facilitate minimally invasive mitral valve surgery.

A educação oceânica é uma estratégia de transformação socioambiental frente as rápidas e profundas mudanças ambientais das últimas décadas. No presente estudo, foi desenvolvido um jogo voltado à educação oceânica denominado “Tarta Certo”, como uma proposta de inovação didática que tem como público alvo crianças e adolescentes. O jogo possui 120 cartas com perguntas que envolvem educação oceânica usando tartarugas marinhas como bandeira, com aspectos da sua biologia, comportamento, ameaças e interações nos ecossistemas marinhos e terrestres. O jogo foi desenvolvido para ser aplicado de forma remota e presencial e os interagentes são incentivados a responder as perguntas podendo acionar as dicas que são as cartas com desenhos que induzem a resposta correta. Com o objetivo de elaborar um material didático acessível e eficiente na sensibilização ambiental, o jogo foi avaliado por 20 professores, os quais apontaram seu caráter inovador, criativo e de potencial interesse e adesão dos alunos para otimizar o processo de ensino-aprendizagem. As inovações nos processos de ensino-aprendizagem são uma das formas de otimizar a educação como plataforma de modificação do atual cenário de ameaças aos ecossistemas. O “Tarta Certo” foi desenvolvido para ser uma alternativa nas práticas ambientais, intermediando os processos de construção cidadã e socioambiental.

O artigo estabelece uma análise comparativa do recorte temporal 2019-2020 relativo ao desempenho dos estudantes de escolas públicas e privadas no Exame Nacional do Ensino Médio (ENEM) no município de Fortaleza. O ENEM se destaca no cenário educacional nacional por ser um relevante instrumento de seleção de candidatos aos cursos de nível superior das universidades. Nesse contexto, problematiza-se: há diferenças de desempenho quando comparados os resultados do ENEM dos estudantes das instituições públicas com os resultados dos estudantes das escolas privadas? A pesquisa justifica-se por colaborar na orientação para decisões pedagógicas mais assertivas quanto ao êxito escolar de maior quantidade dos estudantes. Para identificar possíveis diferenças significativas nos resultados obtidos pelos alunos dos sistemas público e privado fez-se uso de Análise de Variância (ANOVA). Os referidos dados foram obtidos a partir do acesso dos microdados da seção Plano de Dados Abertos do site do Instituto Nacional de Pesquisa (INEP). A partir do levantamento desses dados, buscou-se identificar possíveis diferenças a partir da comparação das médias/medianas do desempenho nas Áreas do Conhecimento entre estudantes oriundos das escolas públicas e privadas do município de Fortaleza.

ContextThe risk stratification of patients with differentiated thyroid cancer (DTC) is crucial in clinical decision making. The most widely accepted method to assess risk of recurrent/persistent disease is described in the 2015 American Thyroid Association (ATA) guidelines. However, recent research has focused on the inclusion of novel features or questioned the relevance of currently included features.ObjectiveTo develop a comprehensive data-driven model to predict persistent/recurrent disease that can capture all available features and determine the weight of predictors.MethodsIn a prospective cohort study, using the Italian Thyroid Cancer Observatory (ITCO) database (NCT04031339), we selected consecutive cases with DTC and at least early follow-up data (n = 4773; median follow-up 26 months; interquartile range, 12-46 months) at 40 Italian clinical centers. A decision tree was built to assign a risk index to each patient. The model allowed us to investigate the impact of different variables in risk prediction.ResultsBy ATA risk estimation, 2492 patients (52.2%) were classified as low, 1873 (39.2%) as intermediate, and 408 as high risk. The decision tree model outperformed the ATA risk stratification system: the sensitivity of high-risk classification for structural disease increased from 37% to 49%, and the negative predictive value for low-risk patients increased by 3%. Feature importance was estimated. Several variables not included in the ATA system significantly impacted the prediction of disease persistence/recurrence: age, body mass index, tumor size, sex, family history of thyroid cancer, surgical approach, presurgical cytology, and circumstances of the diagnosis.ConclusionCurrent risk stratification systems may be complemented by the inclusion of other variables in order to improve the prediction of treatment response. A complete dataset allows for more precise patient clustering.

We report the final analysis, with a 10-year follow-up, of the phase II study GIMEMA CML 0307 (NCT 00481052), which enrolled 73 adult patients (median age 51 years; range, 18-83) with newly diagnosed chronic-phase chronic myeloid leukemia to investigate the efficacy and the toxicity of front-line treatment with nilotinib. The initial dose was 400 mg twice daily; the dose was reduced to 300 mg twice daily as soon as this dose was approved and registered. The 10-year overall survival and progression- free survival were 94.5%. At the last contact, 36 (49.3%) patients were continuing nilotinib (22 patients at 300 mg twice daily, 14 at lower doses), 18 (24.7%) patients were in treatment-free remission, 14 (19.2%) were receiving other tyrosinekinase inhibitors and four (5.5%) patients have died. The rates of major and deep molecular responses by 10 years were 96% and 83%, respectively. The median times to major and deep molecular response were 6 and 18 months, respectively. After a median duration of nilotinib treatment of 88 months, 24 (32.9%) patients discontinued nilotinib while in stable deep molecular response. In these patients, the 2-year estimated treatment-free survival was 72.6%. The overall treatment-free remission rate, calculated on all enrolled patients, was 24.7% (18/73 patients). Seventeen patients (23.3%), at a median age of 69 years, had at least one arterial obstructive event. In conclusion, the use of nilotinib front-line in chronic phase chronic myeloid leukemia can induce a stable treatment-free remission in a relevant number of patients, although cardiovascular toxicity remains of concern.

O presente estudo tem como objetivo articular o processo de constituição da prática educativa das educadoras de creches públicas em relação ao momento da leitura de livros para as crianças. Considerando-se a prática educativa como conjunto de ações desenvolvidas no espaço educativo, elaboradas por e para um sujeito histórico e social, realizamos entrevistas semiestruturadas com educadoras de creches na cidade de João Pessoa-PB que atuam em salas com crianças na faixa etária entre 24 e 35 meses de idade. Os resultados mostraram que a prática educativa das educadoras se caracteriza por meio do planejamento coletivo, visando articular a prática educativa com a habilidade atual da criança, destacando a leitura como um momento da prática que se caracteriza como lúdico, despertando a atenção, o desenvolvimento da imaginação e a criatividade das crianças, podendo ser este momento articulado com outras atividades didático-pedagógicas.

Introduction: Interferon regulatory factor 4 (IRF4) is a transcriptional factor with a key role in the modulation of inflammation and immune surveillance. The IRF4 gene is downregulated in Philadelphia-negative myeloproliferative neoplasms, and its expression is associated with prognosis and response to treatment. Methods: We evaluated the IRF4 expression kinetics during tyrosine kinase inhibitor (TKI) treatment in a cohort of 116 chronic myeloid leukemia (CML) patients to elucidate its role in the disease course. Results: A relationship between the IRF4 expression and the disease burden was observed at various disease stages. A correlation analysis between the International Scale (IS) and IRF4 values confirmed this close association. A significant increase is detected after 3 months of TKI treatment. Patients achieving an early molecular response (EMR) had higher IRF4 values at both diagnosis and after 3 months of therapy as compared to those failing the EMR target. Patients achieving treatment-free remission did not show IRF4 fluctuations during monitoring, while a decreased IRF4 expression emerged at the time of molecular relapse. Conclusion: Our data seem to confirm the relevance of IRF4 in the pathogenesis of CML, suggesting a pivotal role at the disease onset and a predictive value during the CML course.

The effect of ligands' energy levels on thermal dependence of lanthanide emission was examined to create new molecular nanothermometers. A series of Ln2Ga8L8′L8″metallacrowns (shorthand Ln2L8′), where Ln = Gd3+, Tb3+, or Sm3+(H3L′ = salicylhydroxamic acid (H3shi), 5-methylsalicylhydroxamic acid (H3mshi), 5-methoxysalicylhydroxamic acid (H3moshi), and 3-hydroxy-2-naphthohydroxamic acid (H3nha)) and H2L″ = isophthalic acid (H2iph), was synthesized and characterized. Within the series, ligand-centered singlet state (S1) energy levels ranged from 23,300 to 27,800 cm-1, while triplet (T1) energy levels ranged from 18,150 to 21,980 cm-1. We demonstrated that the difference between T1levels and relevant energies of the excited 4G5/2level of Sm3+(17,800 cm-1) and 5D4level of Tb3+(20,400 cm-1) is the major parameter controlling thermal dependence of the emission intensity via the back energy transfer mechanism. However, when the energy difference between S1and T1levels is small (below 3760 cm-1), the S1→ T1intersystem crossing (and its reverse, S1→ T1) mechanism contributes to the thermal behavior of metallacrowns. Both mechanisms affect Ln3+-centered room-temperature quantum yields with values ranging from 2.07(6)% to 31.2(2)% for Tb2L8′and from 0.0267(7)% to 2.27(5)% for Sm2L8′. The maximal thermal dependence varies over a wide thermal range (ca. 150-350 K) based on energy gaps between relevant ligand-based and lanthanide-based electronic states. By mixing Tb2moshi8′with Sm2moshi8′in a 1:1 ratio, an optical thermometer with a relative thermal sensitivity larger than 3%/K at 225 K was created. Other temperature ranges are also accessible with this approach published

Whereas the 18 F-FDG PET/CT pattern of malignant thyroid neoplasia is known, the glucose uptake of autonomously functioning thyroid nodules (AFTNs) has not been fully investigated. We aimed to analyze the FDG uptake of AFTNs and its correlation with clinical, laboratory, ultrasonography, and histological features.We retrospectively analyzed the records of all AFTN patients from 2 centers, in which an 18 F-FDG PET/CT, thyroid function tests, and neck ultrasound were available within a 3-month window from the thyroid scintigraphy. Forty-five patients (35 female patients; median age, 65 years) were retrospectively included. The presence of FDG uptake was compared with the laboratory data and, whenever available, the histology.Over a 36-month follow-up, 20 patients underwent surgery; 4 cancers, 10 follicular adenomas, and 6 follicular hyperplasias were found. Twenty-two AFTNs (48.9%) were FDG-positive, whereas the remaining 23 (51.1%) were not. Thyroid-stimulating hormone (TSH) was significantly lower in FDG-positive AFTNs than in negative ones (0.055 [0.02-0.42] vs 0.65 [0.2-0.96] mIU/L, P = 0.0018). On multivariate analysis, only TSH was independently associated with FDG uptake ( P = 0.008). On receiver operating characteristic curve analysis, TSH0.08 mIU/L indicated FDG-positive AFTNs, with 64% sensitivity, 87% specificity, 4.88 positive likelihood ratio, and 0.42 negative likelihood ratio. In histologically proven benign lesions, TSH was significantly lower in follicular adenomas than in follicular hyperplasias ( P0.001). Patients with cancer had TSH in the low-normal range.Autonomously functioning thyroid nodules show heterogeneous uptake FDG pattern, which depends on histopathology and thyroid function. Particularly, follicular adenomas tend to display increased glucose uptake and suppressed TSH.

This project consists of developing a Eucalyptus-based product. The selected product is a sports performance T-shirt impregnated with eucalyptus essential oil. A Chemical Product Design approach was used to translate customer needs into the new product. This procedure is divided into four stages: Needs, Ideas, Selection and Manufacture. This product’s target audience is athletics and hiking enthusiasts. Its main purpose is to valorize Eucalyptus Globulus essential oil’s unique properties, providing fresh and decongestion sensations to the user. Finally, an economic analysis was performed to evaluate the product viability.

OBJECTIVE Gamma Knife radiosurgery (GKRS) is an effective treatment for vestibular schwannomas (VSs) and has been used in > 100,000 cases worldwide. In the present study the authors sought to define the serial volumetric tumor response of Koos grade I–IV VS after radiosurgery. METHODS A total of 201 consecutive VS patients underwent GKRS at a single institution between 2015 and 2019. All patients had a minimum follow-up of 18 months and at least 2 interval postprocedure MRI scans. The contrast-enhanced tumor volumes were contoured manually and compared between pre- and post-GKRS imaging. The percentages of tumor volume change at 18 months (short-term follow-up) and up to 5 years after GKRS (long-term follow-up) were compared with the baseline tumor volume. An increase of 20% was considered a significant increase of tumor volume. Trends of tumor volume over time were assessed with linear models using time as a continuous variable. A test for linear trend was evaluated according to the initial Koos tumor classification. RESULTS Koos grade II VS was the most frequently occurring tumor (n = 74, 36.8%), followed by grade III (n = 57, 28.4%), grade I (n = 41, 20.4%), and grade IV (n = 29, 14.4%). The mean tumor volume at the time of GKRS was 2.12 ± 2.82 cm3 (range 0.12–18.77 cm3) and the median margin dose was 12 Gy. Short-term follow-up revealed that tumor volumes transiently increased in 34.2% and 28.4% of patients at 6 and 18 months, respectively, regardless of Koos grade. Linear regression analysis of Koos grade II, III, and IV tumors showed a significant longitudinal volume decrease on long-term follow-up. At last follow-up (median 30 months, range 18–54 months), 19 patients (9.4%) showed a persistent increase of tumor volume. Five patients received additional management after GKRS. CONCLUSIONS Although selected VS patients demonstrate an early and measurable transient volumetric increase after GKRS, > 90% have stable or reduced tumor volumes over an observed period of up to 5 years. Volumetric regression is most pronounced in Koos grade II, III, and IV tumors and may not be fully detectable until 3 years after GKRS.

A produção de biscoitos se mostra um ramo pouco estudado, tanto nos processos de fabricação como no gerencial. Várias são as dificuldades quando se trata de produção em larga escala. Esse trabalho teve por objetivo identificar as possíveis influências das matérias-primas e insumos nos processos produtivos de biscoitos laminados. Foram realizados: acompanhamento do processo produtivo, coleta de dados, observações gerais, tabulação e pesquisa teórica. Os resultados da pesquisa permitiram observar as dificuldades impostas à produção devido a farinhas inadequadas, algumas vezes por defeitos em equipamentos, outras devido a problemas com colaboradores. Para a produção de biscoitos padronizados torna-se necessário também, padrão na matéria-prima, no treinamento de funcionários, na administração e manutenção preventiva.

The maintenance of genome integrity is ensured by proper chromosome inheritance during mitotic and meiotic cell divisions. The chromosomal counterpart responsible for chromosome segregation to daughter cells is the centromere, at which the spindle apparatus attaches through the kinetochore. Although all mammalian centromeres are primarily composed of megabase-long repetitive sequences, satellite-free human neocentromeres have been described. Neocentromeres and evolutionary new centromeres have revolutionized traditional knowledge about centromeres. Over the past 20 years, insights have been gained into their organization, but in spite of these advancements, the mechanisms underlying their formation and evolution are still unclear. Today, through modern and increasingly accessible genome editing and long-read sequencing techniques, research in this area is undergoing a sudden acceleration. In this article, we describe the primary sequence of a previously described human chromosome 3 neocentromere and observe its possible evolution and repair results after a chromosome breakage induced through CRISPR-Cas9 technologies. Our data represent an exciting advancement in the field of centromere/neocentromere evolution and chromosome stability.