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© 2014, National Academy of Sciences. All rights reserved. Most inherited forms of blindness are caused by mutations that lead to photoreceptor cell death but spare second- and third-order retinal neurons. Expression of the light-gated excitatory mammalian ion channel light-gated ionotropic glutamate receptor (LiGluR) in retinal ganglion cells (RGCs) of the retina degeneration (rd1) mouse model of blindness was previously shown to restore some visual functions when stimulated by UV light. Here, we report restored retinal function in visible light in rodent and canine models of blindness through the use of a second-generation photoswitch for LiGluR, maleimide-azobenzene-glutamate 0 with peak efficiency at 460 nm (MAG0460). In the blind rd1 mouse, multielectrode array recordings of retinal explants revealed robust and uniform light-evoked firing when LiGluR-MAG0460was targeted to RGCs and robust but diverse activity patterns in RGCs when LiGluR-MAG0460was targeted to ON-bipolar cells (ON-BCs). LiGluR-MAG0460in either RGCs or ON-BCs of the rd1 mouse reinstated innate light-avoidance behavior and enabled mice to distinguish between different temporal patterns of light in an associative learning task. In the rodcone dystrophy dog model of blindness, LiGluR-MAG0460in RGCs restored robust light responses to retinal explants and intravitreal delivery of LiGluR and MAG0460was well tolerated in vivo. The results in both large and small animal models of photoreceptor degeneration provide a path to clinical translation.