7 results on '"Aditi Mitra Puja"'
Search Results
2. Ginsenoside compound K-loaded gold nanoparticles synthesized from Curtobacterium proimmune K3 exerts anti-gastric cancer effect via promoting PI3K/Akt-mediated apoptosis
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Aditi Mitra Puja, Xingyue Xu, Rongbo Wang, Hoon Kim, and Yeon-Ju Kim
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Oncology ,Biomedical Engineering ,Pharmaceutical Science ,Physical and Theoretical Chemistry - Abstract
Background Compound K (CK) is the minor ginsenoside present in fermented Panax ginseng extract. Despite the pharmacological efficacy of CK, its industrial use has been restricted due to its low water solubility and poor permeability. To overcome this defect, our study was to synthesize gold nanoparticles from CK (CK-AuNPs) to investigate their potential as anticancer candidates. Methods To biologically synthesize CK-AuNPs, a novel strain, Curtobacterium proimmune K3, was isolated from fermented ginseng beverage, then combined with CK and gold salts to biosynthesize gold nanoparticles (CurtoCK-AuNPs). Their physicochemical characteristics were evaluated using UV–Vis spectrometry, FE-TEM, EDX, elemental mapping, XRD, SAED, DLS and TGA. Results CurtoCK-AuNPs exerted significant selective cytotoxic effects on AGS human gastric cancer cells. Fluorescence staining with Hoechst, propidium iodide, and MitoTracker demonstrated that CurtoCK-AuNPs induce apoptosis and mitochondrial damage, respectively. Quantitative real-time PCR and western blotting analyses showed that cytotoxic effect of CurtoCK-AuNPs were involved in apoptosis, based on their activation of Bax/Bcl-2, cytochrome c, caspase 9, and caspase 3, as well as their suppression of PI3K–Akt signaling. Conclusion Our findings provide data for understanding the molecular mechanisms of nanoparticles; thus, providing insight into the development of alternative medications based on gold nanoparticles of ginseng-derived CK.
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- 2022
3. Development of a molecular marker based on chloroplast gene for specific identification of Korean Hibiscus (Hibiscus syriacus ‘Simbaek’)
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Rongbo Wang, Sang Yong Park, Sul Woong Park, Aditi Mitra Puja, and Yeon-Ju Kim
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Organic Chemistry ,General Biochemistry, Genetics and Molecular Biology - Abstract
Due to the rise in substitution and adulteration of herbal products, as well as the lack of genetic information on Hibiscus plants, more molecular markers are needed to understand the genetic diversity and avoid their misidentification. There are many allelic variants of the functional genes in Hibiscus and other plants, which control their respective phenotypes and other characteristics. Identifying alleles of the desired trait by determining diversification through gene-typing allele-specific markers for authentication is, therefore, a potent strategy. The purpose of this study was to use insertion/deletion (InDel) markers to identify Hibiscus syriacus cultivars. We developed a novel InDel marker for Korean Hibiscus ‘Simbaek’, based on the trnL-F region of the chloroplast gene. Through this InDel site, a modified specific primer pair and a novel multiplex polymerase chain reaction (PCR) system were developed for specific identification of the Korean Hibiscus Simbaek cultivar. The molecular markers developed in this study were highly specific and accurately authenticated as Simbaek from the five main cultivars of H. syriacus. Taken together, the described method is a potential tool for the identification and selection of germplasm resource of Simbaek cultivar. Graphical Abstract
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- 2021
4. Nanoemulsions prepared from mountain ginseng-mediated gold nanoparticles and silydianin increase the anti-inflammatory effects by regulating NF-κB and MAPK signaling pathways
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Thi Hoa My Tran, Aditi Mitra Puja, Hoon Kim, and Yeon-Ju Kim
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Lipopolysaccharides ,Spectroscopy, Fourier Transform Infrared ,Anti-Inflammatory Agents ,NF-kappa B ,Metal Nanoparticles ,Panax ,Gold ,Signal Transduction ,Silymarin - Abstract
In order to increase the bioavailability of mountain ginseng (MG), gold nanoparticles (MG-AuNPs) were biologically synthesized from MG extract, and an oil-in-water (O/W) nanoemulsion (SMG-AuNEs) was prepared from MG-AuNPs and a phytochemical silydianin. The physical stability of SMG-AuNEs were monitored and optimized in terms of particle size, pH value, zeta potential, and polydispersity index. The chemicostructural properties of the prepared MG-AuNPs and SMG-AuNEs were characterized using various spectrometric and microscopic analyses, such as EDX spectroscopy, FT-IR spectroscopy, and TEM. The effect of both nanomaterial samples on the anti-inflammatory activity and their underlying mechanism was compared in LPS-stimulated RAW 264.7 cells. SMG-AuNEs did not show toxic effects against RAW 264.7 macrophages, HaCaT keratinocytes, and normal dermal fibroblasts. SMG-AuNEs exhibited significantly higher inhibition of pro-inflammatory genes and proteins, including IL-1β, IL-6, and TNF-α, compared with those of MG-AuNPs and silydianin. Western blotting analysis revealed that the MAPK and NF-κB signalings were highly inhibited by SMG-AuNEs treatment. Hence, this study shows that nano-emulsification of gold nanoparticles prepared from MG is a useful method for augmenting the anti-inflammatory potential of MG. This study may serve as a foundation for using MG as a functional ingredient in anti-inflammatory agents. Our results may implicate the use of nanoemulsions to develop new anti-inflammatory products using MG.
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- 2022
5. Gold Nanoparticles Prepared with
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Rongbo, Wang, Xingyue, Xu, Aditi Mitra, Puja, Haribalan, Perumalsamy, Sri Renukadevi, Balusamy, Hoon, Kim, and Yeon-Ju, Kim
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autophagy ,gold nanoparticles ,gastric cancer ,Phyllanthus emblica fruit ,apoptosis ,Bifidobacterium animalis subsp. lactis ,Article - Abstract
(1) Background: Nanotechnology is being widely applied for anticancer strategies with few side effects. Nanoparticles (NPs) prepared from natural extracts are promising candidates for cancer treatment because of their unique physicochemical characteristics. This study aimed to prepare gold nanoparticles (AuNPs) from Phyllanthus emblica fruit extract (PEFE) using Bifidobacterium animalis subsp. lactis (B. lactis) and to evaluate their anticancer activity against the human gastric adenocarcinoma cell-line (AGS). (2) Methods: The safety of microbial biosynthesis AuNPs (PEFE-AuNPs) was assessed by evaluating the cytotoxicity. The anticancer activity of PEFE-AuNPs was investigated in AGS cells in terms of apoptosis and autophagy. (3) Results: PEFE-AuNPs exhibited significant cytotoxicity against AGS cells but not against normal cells. The apoptosis induced by PEFE-AuNPs in AGS cells was associated with PTEN-induced kinase 1 (PINK1)-Parkin mediated reduction of mitochondrial membrane potential and activation of intracellular signaling apoptosis pathways. The anticancer activity of PEFE-AuNPs was associated with induction of apoptosis through inhibition of autophagy, downregulation of LC3-II/LC3-I and Beclin-1 expression, and upregulation of p62 expression in AGS cells. (4) Conclusions: This study is the first to demonstrate the anticancer activity of PEFE-AuNPs against AGS cells. Our results provide a good starting point for the development of new anticancer products based on gold nanoparticles of P. emblica fruit extract.
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- 2021
6. Biosynthesis of gold nanoparticles using Nigella sativa and Curtobacterium proimmune K3 and evaluation of their anticancer activity
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Hoon Sik Kim, Sri Renukadevi Balusamy, Yeon-Ju Kim, Haribalan Perumalsamy, Rongbo Wang, Xingyue Xu, Sanjeevram Dhandapani, and Aditi Mitra Puja
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Cell signaling ,Materials science ,Metal Nanoparticles ,Bioengineering ,Caspase 3 ,Apoptosis ,02 engineering and technology ,010402 general chemistry ,Caspase 8 ,01 natural sciences ,Biomaterials ,Cell Line, Tumor ,Autophagy ,Humans ,Nigella sativa ,Cytotoxicity ,chemistry.chemical_classification ,Reactive oxygen species ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Cell biology ,chemistry ,Mechanics of Materials ,Biosynthetic process ,Gold ,0210 nano-technology ,Reactive Oxygen Species - Abstract
In recent times, the development of functionalized nanoparticle methodology for biomedical applications has become a major challenge. In the present study, we prepared a novel gold nanoparticle (AuNP), named Curto-Cumin AuNP (CC-AuNP), using the biosynthetic process involving Nigella sativa (black cumin) seed extract and membrane vesicles isolated from the novel probiotic strain, Curtobacterium proimmune K3. Various spectrometric and microscopic analyses were performed to characterize the physicochemical properties of the nanoparticles. CC-AuNP exhibited significant cytotoxicity against human gastric adenocarcinoma (AGS) cells but not against normal cells. The toxic effects of the nanoparticles were associated with the excessive production of reactive oxygen species (ROS) in damaged mitochondria. Further, we investigated the molecular mechanisms underlying the cytotoxic effect of CC-AuNP. Results showed that except for B cell lymphoma 2 (Bcl-2), the intracellular apoptotic signaling molecules, such as p53, Bcl-associated X protein (Bax), and Caspase 9/Caspase 3 were significantly upregulated in AGS cells. ROS production and alterations in mitochondrial membrane potential were observed in AGS cells treated with CC-AuNP. The activation of autophagy flux-related biomarkers, such as LC3b/a, Beclin-1, p62, and Caspase 8, was confirmed by qPCR and western blotting. Autophagy pathway was suppressed in CC-AuNP-treated AGS cells and could not proceed further to the mature state. This was confirmed by the evaluation of both apoptosis and autophagy signaling pathways using autophagy-induced AGS cells treated with rapamycin, a well-studied autophagy activator. Overall, our results showed that CC-AuNP upregulates apoptotic signaling and suppresses the autophagy-related signaling pathway, and thus has potential as an anticancer agent. To our knowledge, the present study is the first to demonstrate that CC-AuNP may serve as novel therapeutic agent against gastric cancer. Furthermore, our study provides preliminary data which can be used to develop novel anticancer candidates and understand their anticancer mechanisms, and seems to be a good starting point for the development of alternative medications based on CC-AuNP.
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- 2021
7. Gold Nanoparticles Prepared with Phyllanthus emblica Fruit Extract and Bifidobacterium animalis subsp. lactis Can Induce Apoptosis via Mitochondrial Impairment with Inhibition of Autophagy in the Human Gastric Carcinoma Cell Line AGS
- Author
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Yeon-Ju Kim, Xingyue Xu, Hoon Sik Kim, Aditi Mitra Puja, Sri Renukadevi Balusamy, Haribalan Perumalsamy, and Rongbo Wang
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autophagy ,General Chemical Engineering ,PINK1 ,Pharmacology ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Bifidobacterium animalis subsp. lactis ,General Materials Science ,Cytotoxicity ,QD1-999 ,030304 developmental biology ,0303 health sciences ,biology ,Kinase ,Chemistry ,gastric cancer ,Autophagy ,apoptosis ,biology.organism_classification ,Bifidobacterium animalis ,Cell culture ,Apoptosis ,gold nanoparticles ,030220 oncology & carcinogenesis ,Phyllanthus emblica fruit - Abstract
(1) Background: Nanotechnology is being widely applied for anticancer strategies with few side effects. Nanoparticles (NPs) prepared from natural extracts are promising candidates for cancer treatment because of their unique physicochemical characteristics. This study aimed to prepare gold nanoparticles (AuNPs) from Phyllanthus emblica fruit extract (PEFE) using Bifidobacterium animalis subsp. lactis (B. lactis) and to evaluate their anticancer activity against the human gastric adenocarcinoma cell-line (AGS). (2) Methods: The safety of microbial biosynthesis AuNPs (PEFE-AuNPs) was assessed by evaluating the cytotoxicity. The anticancer activity of PEFE-AuNPs was investigated in AGS cells in terms of apoptosis and autophagy. (3) Results: PEFE-AuNPs exhibited significant cytotoxicity against AGS cells but not against normal cells. The apoptosis induced by PEFE-AuNPs in AGS cells was associated with PTEN-induced kinase 1 (PINK1)-Parkin mediated reduction of mitochondrial membrane potential and activation of intracellular signaling apoptosis pathways. The anticancer activity of PEFE-AuNPs was associated with induction of apoptosis through inhibition of autophagy, downregulation of LC3-II/LC3-I and Beclin-1 expression, and upregulation of p62 expression in AGS cells. (4) Conclusions: This study is the first to demonstrate the anticancer activity of PEFE-AuNPs against AGS cells. Our results provide a good starting point for the development of new anticancer products based on gold nanoparticles of P. emblica fruit extract.
- Published
- 2021
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