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Background: The new resurgence of human Monkeypox (Mpox) cases, both within and outside of the central and western African countries, is causing concern among public health officials worldwide. Pediatric physicians play an important role in Mpox surveillance and case management; thus, this study aims to determine the level of Mpox knowledge among pediatric residents in Saudi Arabia. Methods: A cross-sectional study was done from November 21 to December 28, 2022, in Riyadh, the capital of Saudi Arabia involving 128 pediatric residents' physicians chosen through systematic random sampling. A validated, semi-structured, self-administered questionnaire was used to collect data. R-software was used to analyze the data. The chi-square test was used to investigate the relationship between the knowledge score and the sociodemographic independent variables. Results: Among the 128 pediatric residents' physicians who participated in this study, the majority (65.7%) were aged >26 years. More of the respondents were female (57%), married (63.2%), and in their junior level of residency years (48.4%). The majority (66%) of the respondents have good knowledge of Mpox. Being a senior resident, getting a full dose of COVID-19 vaccines, and having recently heard about Mpox outbreaks were statistically significant factors associated with good scores in knowledge about Mpox. The main sources of information were medical education, radio, television, and newspaper. Conclusions: Despite the adequate level of knowledge about the Mpox gap among pediatric residents' physicians, it is recommended that targeted intervention could be a channel through organized continuous medical education and social media to improve the knowledge about any new emergent diseases.

Purpose. The purpose was to investigate the effect of a psycholinguistic training to improve expressive language among children with ASD. Methods. Data were collected from children during the academic year 2021-2022. The participants were 20 children with ASD, aged 7 to 10 years, who were enrolled in a school for children with ID. All children attended the same semester inside the school. Parent consent forms were sent home by the principal and school psychologist to the parents of the prospective participants to inform them about the study and ask them to give permission for their children to participate. Demographic information was obtained from school records and as reported by families. Children were divided randomly into two groups: one experimental (n=10) and other was the control group (n=10). These children were matched as a group with cases on the basis of age, IQ, social class and general level of language ability. A 24 items, six-dimensional Questionnaire was developed in particular for this study. The intention was to identify expressive language skills for children with ASD. The six dimensions are: speaking and questioning skills in correct language (4 items), the ability to verbally express things, needs, and desires (4 items), the skill of pronouncing and imitating words, letters and sounds (4 items), repetition and arranging skill (4 items), the ability to verbally express feelings (4 items), the ability to respond to the speech and questions of others. Results. Results indicated increased speaking and questioning skills in correct language, the ability to verbally express things, needs, and desires, the skill of pronouncing and imitating words, letters and sounds, repetition and arranging skill, the ability to verbally express feelings and the ability to respond to the speech and questions of others. Conclusions. Overall, results from this study contribute to the growing literature on the effect of a psycholinguistic training to improve expressive language among children with ASD. The present study lends empirical support to the notion that expressive language of children with ASD can be improved through a psycholinguistic training.

Purpose: The present study aimed to assess the effect of RF and CP intervened PDT and Er, Cr: YSGG laser on microleakage in class V RMGIC restorations in permanent teeth. Materials and Methods: In-vitro eighty standardized class V cavities (4×4×2) were prepared in extracted permanent molars based on ICDAS II (International Caries Detection and Assessment System II) caries severity code 0. All samples were arbitrarily alienated into four groups: Group 1, PDT mediated riboflavin photosensitizer (RF) [concentration of 150 μg/mL]; Group 2, PDT with curcumin photosensitizer (CP) [concentration of 100 μg/mL]; Group 3, Er, Cr: YSGG laser application; Group 4, control group: rinsed and dried dentin. Cavities were restored with conventional RMGIC. The specimens were immersed in 0.5% Fuchsine solution for 24 hrs. and sectioned. Microleakage scores were assessed under a microscope. Intergroup and intragroup comparisons were made at enamel and cementum margin respectively. Data were analyzed by non-parametric Kruskal-Wallis test and Wilcoxon tests at the level of significance deemed p < 0.05. Results: Riboflavin and curcuminmediated PDT would produce a superior reduction (95% viz 90%) of microleakage as compared to Er, Cr: YSGG laser (85%), and no control groups. Cu-PDT observed the highest amount of microleakage with the least amount of microleakage in the control group. Conclusion: Riboflavin and curcumin photosensitizers mediated PDT and Er, Cr: YSGG laser has the potential to be utilized in class V cavities before RMGIC restorations at enamel margins without adversely affecting the marginal integrity.

Purpose. The aim of this study was to investigate the contribution of rapid automatized naming skills (RAN) and phonological awareness (PA) to Arabic language reading fluency(RF). Methods. A quantitative, cross-sectional and descriptive method was employed, with the main focus on the contribution of rapid automatized naming skills and phonological awareness to Arabic language reading fluency. A cross-sectional study was performed for all children in six prim art schools located in Makka. A convenience sampling method was used to recruit children. For children to be included in this study, there were some criteria: (a) to have no academic or developmental disabilities; (b) Both gender (males and females). Questionnaires were distributed to the children with the help of classroom teachers. All questions must be answered and responded to. A total of 360 participants aged 6–10 years took part in this study: 280 males (77.7%, age mean = 8.9, SD = 3.27), and 80 females (22.3%, age mean = 9.2, SD = 2.44). Results. The first main finding of the current research is that the correlations between RAN, PA and reading fluency were significant. In the final model, PA was directly and positively predictive of RAN (β = 0.664, p = 0.000) and RAN was directly and strongly related to RF (β = 0.623, p = 0.000). PA explained 52.3% of variance of RF. RAN explained 51.8% of variance of RF. PA and RAN together explained 69.9% of variance of RF. Conclusions. The findings of this study add to our knowledge of the contribution of PA and RAN to RF, as well as the complex intralingual relationship between PA and RAN.

This study aims to evaluate the feasibility of chlorhexidine (CHX)-loaded poly-L-glycolic acid (PLGA) nanoparticles as a modifier of a commercial orthodontic adhesive via the assessment of physicochemical, biological, and mechanical properties at tooth-bracket interface. CHX-loaded PLGA nanoparticles were synthesized using double emulsion-solvent evaporation method and characterized using transmission electron microscopy and Raman analysis. CHX-loaded PLGA nanoparticles in Transbond XT orthodontic adhesive were prepared using two different concentrations of the CHX (25 and 50%) and characterized for degree of conversion (DC), antimicrobial, and cytotoxicity testing. Bonded specimens were tested for shear bond strength (SBS) and adhesive remnant index (ARI) at tooth-bracket interface. The synthesized PLGA nanoparticles averaged between 60 and 80 nm in size. After loading CHX inside PLGA nanoparticles, the morphology of the PLGA nanoparticles was considerably changed. Orthodontic bracket bonded with 25% CHX-loaded PLGA-modified adhesive demonstrated DC scores similar to control group. Both 25 and 50% CHX-loaded PLGA-modified adhesive specimens showed higher antibacterial activity against S. mutans compared to control group. The least mean SBS values were exhibited by 50% CHX-loaded PLGA-modified adhesive samples, while a statistically significant difference was observed in the mean ARI values among all study groups at all-time points (p = .018). This study indicates that the addition of CHX-loaded PLGA nanoparticles in Transbond XT achieved stable bonds with enhanced antimicrobial and mechanical properties.

Anthroponotic cutaneous leishmaniais (ACL) and zoonotic cutaneous leishmaniasis (ZCL) caused by

The eradication rates for Helicobacter pylori globally are decreasing with a dramatic increase in the prevalence of antibiotic resistant bacteria all over the world, including Saudi Arabia. There is no current consensus on the management of H. pylori in Saudi Arabia. The Saudi Gastroenterology Association developed these practice guidelines after reviewing the local and regional studies on the management of H. pylori. The aim was to establish recommendations to guide healthcare providers in managing H. pylori in Saudi Arabia. Experts in the areas of H. pylori management and microbiology were invited to write these guidelines. A literature search was performed, and all authors participated in writing and reviewing the guidelines. In addition, international guidelines and consensus reports were reviewed to bridge the gap in knowledge when local and regional data were unavailable. There is limited local data on treatment of H. pylori. The rate of clarithromycin and metronidazole resistance is high; therefore, standard triple therapy for 10-14 days is no longer recommended in the treatment of H. pylori unless antimicrobial susceptibility testing was performed. Based on the available data, bismuth quadruple therapy for 10-14 days is considered the best first-line and second-line therapy. Culture and antimicrobial susceptibility testing should be considered following two treatment failures. These recommendations are intended to provide the most relevant evidence-based guidelines for the management of H. pylori infection in Saudi Arabia. The working group recommends further studies to explore more therapeutic options to eradicate H. pylori.

A framing analysis of eight US media with different political bias was done to identify how they positioned themselves regarding the role of China in Covid-19 in the USA. The number of articles in the media varied much with Los Angeles Times leading with 32 articles discovered in the search. About 76% of all articles in the media were negative to China and 24% were sympathetic and only one article (American Conservative) saw the benefit of economic contribution of China to the USA. Both maximum negative and positive articles were seen in Los Angeles Times. All of the articles in the Star Tribune were negative and close to 95% in US today, also. However, the total numbers of articles in these two were only 15 and 19 respectively. Majority of articles in both right-centered and left-centered media were negative with obvious sympathetic stand of left-centered media towards China. On this basis, no definite association between political bias and positive or negative reporting on the role of China in Covid-19 spread in the USA exists for the eight US media studied. The results have been discussed with the aid of other works. Some limitations of this study are also discussed at the end.

Neurodegeneration is a prevalent and one of the emerging reasons for morbidity, mortality, and cognitive impairment in aging. Dementia is one of such conditions of neurodegeneration, partially manageable, irreversible, and worsens over time. This review is focused on biological and psychosocial risk factors associated with Alzheimer's and Parkinson's diseases, highlighting the value of cognitive decline. We further emphasized on current therapeutic strategies from pharmacological and non-pharmacological perspectives focusing on their effects on cognitive impairment, protein aggregation, tau pathology, and improving the quality of life. Deeper mechanistic insights into the multifactorial neurodegeneration could offer the design and development of promising diagnostic and therapeutic strategies.

Anthroponotic cutaneous leishmaniais (ACL) and zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania tropica and Leishmania major, respectively, are endemic vector-borne diseases in southern Saudi Arabia. In 2021, an outbreak of cutaneous leishmaniasis occurred in the province of Asir. The main objective of our investigation was to analyze the epidemiological features of CL in southern Saudi Arabia. The ministry of health recorded 194 CL patients between January and December 2021 from the Asir province. Our findings showed that the majority of CL patients (87.1%) originated from the governorates of Khamis-Mushait and Abha. Most of the patients were males (62.3%). While CL affected all age groups, those under 13 years old were the most affected (38.1%). For both genders, CL patients were mostly Saudi citizens (90.7%) compared to non-Saudi expatriates. The majority of CL patients (75.2%) suffered from a single lesion, and the majority of lesions (61.3%) were located on the face. The seasonal prevalence of CL showed two peaks, a small one in July–August and a larger one in March. Of a total of 194 Giemsa slides samples, 188 showed positive amplification of Leishmania ITS1 gene. Based on PCR-RFLP and PCR-HMR, 183 patients showed positive amplification of L. tropica and five patients showed positive amplification of L. major. Phylogenetic analysis revealed a clear distinct separation between L. major and L. tropica sequences. Our results provided strong evidence of the pre-domination of L. tropica, the main etiological agent of ACL in Asir province. We reported for the first time the presence of L. major, an etiological agent of ZCL in the study areas. The co-circulation of ACL and ZCL highlighted the complexity of the epidemiology of CL in southern Saudi Arabia, and subsequently, further studies to identify competent vectors and reservoir hosts for the establishment of control strategies are needed.

Monogenic diseases that result in early pregnancy loss or neonatal death are genetically and phenotypically highly variable. This often poses significant challenges in arriving at a molecular diagnosis for reproductive planning. Molecular autopsy by proxy (MABP) refers to the genetic testing of relatives of deceased individuals to deduce the cause of death. Here, we specifically tested couples who lost one or more children/pregnancies with no available DNA. We developed our testing strategy using whole exome sequencing data from 83 consanguineous Saudi couples. We detected the shared carrier state of 50 pathogenic variants/likely pathogenic variants in 43 families and of 28 variants of uncertain significance in 24 families. Negative results were seen in 16 couples after variant reclassification. In 10 families, the risk of more than one genetic disease was documented. Secondary findings were seen in 10 families: either genetic variants with potential clinical consequences for the tested individual or a female carrier for X-linked conditions. This couple-based approach has enabled molecularly informed genetic counseling for 52% (43/83 families). Given the predominance of autosomal recessive causes of pregnancy and child death in consanguineous populations, MABP can be a helpful approach to consanguineous couples who seek counseling but lack molecular data on their deceased offspring.

Given that teaching Arabic, as a second language has become increasingly significant in the present context, it follows that there is an urgent and pressing need to develop efficient learning tools as well as adequate measuring tools for testing the learner’s development. There are numerous problems associated with measuring a learner’s proficiency in Arabic in the context of Western cultures. These problems are related to the non-adaptability of measuring tools from one setting into another without taking cultural factors into account. The difficulties faced by scholars in adapting Teaching English to Speakers of Other Languages (TESOL) tools to the Saudi context is an example. However, the problems associated with such an adaptation indicate the need for context-specific language acquisition measuring tools. Either currently existing tools such as TESOL need to be radically altered to fit Saudi contexts and requirements, or entirely new tools must be created in order to test the efficacy of language learning in Saudi Arabia. This study aims at a close examination of ways in which existing tests such as TESOL may be adapted or modified to suit the requirements of teachers and learners in the Saudi context. A survey and evaluation of existing tools was followed by developing new tools specifically for Arabic language. It concludes by giving recommendations for proposed modification of existing strategies for Arabic learners that associates the language more directly with functional workplace contexts.

This within subject clinical experiment assessed oral health impacts before and after Invisalign orthodontic treatment and their relationships with personality characteristics. 50 patients (26 females and 24 males; mean age = 27.62 ± 8.25 years, SE = 1.17, 95% CI = 24.71–29.89 years) were assessed before and after treatment with Invisalign orthodontic treatment. Treatment clinical success was evaluated according defined clinical guidelines. Oral health impacts before and after Invisalign orthodontic treatment were measured via the Oral Health Impact Profile (OHIP). Personality features were measured via the NEO Five-Factor Inventory (NEO-FFI). Probability of α = .05 was utilized to identify significant findings. Females scored less OHIP scores after treatment (had less negative impacts) in comparison to baseline OHIP scores (t = 3.782, df = 25, P = .001, 95% CI of mean difference = 2.750–9.327). Among males, openness scores (R2 = .911, B = 5.235, 95% CI for B = 0.062–10.407, t = 2.601, P = .048) were able to predict OHIP scores before treatment; meanwhile, extraversion (R2 = .959, B = − 8.224, 95% CI for B = − 14.605–1.843, t = − 3.313, P = .021), openness (R2 = .959, B = 21.795, 95% CI for B = 10.737–32.853, t = 5.067, P = .004), and conscientiousness (R2 = .959, B = 10.293, 95% CI for B = 4.796–15.790, t = 4.813, P = .005) scores were useful to predict OHIP scores after treatment (R2 = .959, P P > .05). These findings demonstrate that oral health impacts of Invisalign orthodontic treatment and personality profiles contribution to oral health impacts were different between genders.

The aim of the present clinical trial is to evaluate the clinical, bacterial, and immunological outcomes of photodynamic therapy (PDT) in adolescent patients with orthodontic treatment-induced gingival enlargement (OTGE).A total of 26 adolescent patients, 16 girls and 10 boys, of mean age 16.8 years, undergoing fixed orthodontic treatment for at least 6 months (mean duration in months, 8.3 ± 1.9) and presenting with GE were included in the clinical trial. All patients undergoing fixed orthodontic treatment with clinical diagnosis of OTGE were divided into two groups: Patients receiving full-mouth periodontal debridement (FMPD) 'Group-FMPD' and 'Group-PDT' included participants receiving methylene blue-assisted PDT in the selected area along with FMPD. Clinical periodontal assessments included the evaluation of plaque scores (PS), bleeding on probing (BOP), probing depth (PD) and hyperplastic index (HI). The total bacterial counts of Porphyromonas gingivalis (P. gingivalis), Treponema denticola (T. denticola), and Tannerella forsythia (T. forsythia) were estimated and expressed as log CFU/mL of each bacteria. Cytokines including interleukin (IL)-1β and IL-6 were analyzed using enzyme-linked immunosorbent assay (ELISA) kit.The mean percentage of PS and BOP statistically significantly reduced with both FMPD and PDT from baseline to 4 weeks (p0.05). Although mean PD significantly reduced in both the groups at all time points, there was significantly higher reduction with PDT as compared to FMPD at 2 weeks post treatment (p0.05). Inter-group comparison showed PDT demonstrated significantly higher HI at 2- and 4-weeks post treatment (p0.05). The mean log CFU/mL of P. gingivalis and T. forsythia showed statistically significantly higher reduction with PDT than FMPD (p0.05) at both 2-and 4-weeks follow-up. T. denticola reported significantly reduced levels at 2 weeks follow-up period only with PDT (p0.05). No statistically significant difference appeared when both treatment modalities were compared with one another (p0.05). Four weeks after periodontal treatment, IL-6 significantly reduced with PDT compared to FMPD (p0.05).PDT assisted in reducing bacterial counts and improving hyperplastic index in OTGE.

The aim of this paper was to evaluate the contentions and evidence supporting two divergent views about the existence of advantages offered by bilingualism. It also considered whether a definite conclusion was possible. A qualitative research methodology using the published evidence was adopted. The published evidence supporting or rejecting the advantages of bilingualism was collected using search terms in the Google Scholar search engine. A total number of 64 papers were collected, among which only five challenged the claimed advantages enjoyed by bilingual individuals. The papers were classified by their orientation and discussed. Based on the points derived from the evaluation of evidence, some conclusions were drawn. The overwhelming research support in favour of the existence of supposed bilingual advantage tends to suggest a conclusion in favour of that hypothesis. However, the points raised by critics, such as small sample sizes, inadequate matching of other variables, as well as defective measurements and analysis cannot be ignored as they question the very validity of the studies which support bilingual advantage. Future research needs to pay more attention to these aspects.

The aim of this review was to critically evaluate the research works done on power distance and individualism-collectivism in English as a foreign language (EFL) and other types of English language learning. There is a more or less agreement on what happens to teaching and learning processes in high and low power distance (PD) classrooms. However, there are some findings suggesting thesimultaneous existence of both individualism and collectivism (e.g. North Vietnam). In that case, individualism and collectivism have separate identities, rather than being mutually exclusive parts of a single dimension. There are some works in which Hofstede’s(1983, 1986) cultural dimensions could not explain observed differences in thelearning environment. There are possibilities of learning-specific new sets of cultural dimensions in classrooms due to the attitudinal changes of students compared to the average culture of the general population. In many works, a variety of approaches has been suggested for the teacher either to adapt to the culture of the classroom or to use methods of low PD and individualistic methods in high PD-collectivist classrooms in a cautious manner. Communicative Language Teaching (CLT) seems an effective alternative to Grammar-Translation Method (GTM). Contradictory findings have been reported on Japanese classrooms. In one, the students desire to study independently, which the high PD and the collectivist culture of Japan does not allow. Two, for the Japanese learners, group working is more effective due to the collectivist culture. The contradictions involved in the two contentions need to be resolved through more research.

The aim of this review was to evaluate certain methodological issues related to the measurement of cultural dimensions and their effects with special reference to educational contexts. Using Google Scholar with the exact topics as search terms, 50 research reports were obtained. The results obtained from the critical analysis of the methodologies used in these reports are discussed in this review. Most studies were on power distance and individualism/collectivism dimensions of culture. A large number of conceptual frameworks and as many as 121 measurement instruments have been proposed by different workers. Research approach in any particular study is determined by the framework and the instrument chosen or self-developed instruments. Surveys and interviews are more commonly used. Not estimating internal consistency or reliability tests in some works makes it difficult to judge the validity of the method of data analysis. Generally the sample size is in the range of 200-500 participants in surveys. Small sample sizes used in a few works may affect the validity and applicability of their findings. It is concluded that large groups of scientists doing a common research programme across several countries is better than individual researches. Meta-analyses are also superior to individual researches. Such methods ensure validity and replicability of the findings. Although none of the research used scenario analysis, it is also a promising method.

Additional file 1. Raw data used to determine the MIC and MBIC of cinnamon water extract on S. mutans biofilm and the Combined effect of cinnamon water extract (2.5 mg/ml) and nicotine on S. mutans total growth absorbance, planktonic growth and biofilm formation.

The aim of the present study was to evaluate the effectiveness of photodynamic therapy (PDT) on clinical gingival inflammatory parameters, bacterial load, proinflammatory cytokine status, and pain scores in adolescent patients undergoing fixed orthodontic treatment with gingivitis.Thirty adolescent undergoing fixed orthodontic treatment with gingivitis were randomly divided into two groups: Group A - patients undergoing dental scaling (DS) with adjunctive photodynamic therapy (PDT) and Group BDS alone. Clinical gingival parameters including plaque scores, (PS), bleeding on probing (BOP) and probing depth (PD) were assessed. Visual analogue scale (VAS) and McGill Pain Questionnaire (MPQ) were used to report the values of present pain intensity. Crevicular fluid was sampled to quantify the levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α using enzyme-linked immunosorbent assay. The total bacterial counts for Porphyromonas gingivalis (P. gingivalis) and Tannerella forsythia (T. forsythia) were assessed. All examinations were performed at baseline, 1 week and 4 weeks.Both the treatment regimens reported statistically significant reduction in PS and BOP (p0.05). However, no significant difference was observed in PS and PD values when Group B was compared with Group A (p0.05). There was a statistically significant reduction noted for BOP in Group B when compared to Group A (p0.05). There was a statistically significant reduction in the microbial counts of T. forsythia in Group A when compared to Group B at week-1 (p0.05). During the 4-week follow-up, the counts for T. forsythia significantly increased. No significant changes could be seen in either of the pain scores in both the groups (p0.05). Both groups showed statistically significant reduction in the IL-6 and TNF-α levels. However, IL-6 was significantly reduced at 1 week, while TNF-a significantly reduced at 4 weeks of follow up among the participants in Group B.PDT has a positive effect in significantly reducing the periodontal microbial load in established gingivitis in adolescent patients undergoing fixed orthodontic treatment.

Purpose The aim of the present randomized controlled clinical trial was to evaluate the efficacy of photodynamic therapy (PDT) on halitosis in adolescent patients undergoing fixed orthodontic treatment. Materials and methods Forty-five adolescents with halitosis undergoing orthodontic treatment were equally randomized into three groups: Group – I: provision of treatment through PDT on dorsum of tongue; Group – II: provision of treatment with the help of tongue scrappers (TS); Group – III: provision of treatment with the help of TS and adjunctive PDT. Presence of halitosis confirmed on the basis of gas chromatography and estimation of oral malodour with a cysteine challenge and H2S values recorded ≥112 parts per billion (ppb). A portable device Oral Chroma™ was used for performing breath analysis. A total of 5 bacterial species including P. gingivalis, F. nucleatum, P. intermedia, T. forsythia and T. denticola were studied as the proportion of sites defined as having ≥1.0 × 105 bacterial cells. Results The mean age of the participants in Group-I, II and III were 15.4 years, 13.8 years, and 14.2 years, respectively. The median values for Group-I, II and III at initial breath analysis were 874 ppb, 613 ppb and 1089 ppb, respectively. After treatment with the respective therapeutic modalities, a statistically significant difference was seen among groups after 2-weeks (p Table 3 ). After applying Kruskal-Wallis test, group-III showed the highest amount of reduction in H2S concentration (100 %) at final 2-weeks breath analysis compared to group-I and group-II (p = 0.0001). Descriptive statistics for all bacteria showed that group-III (TS + PDT) statistically significantly reduced the proportional distribution of all bacteria at 2-week follow-up (p Conclusion PDT along with tongue scrapping showed effective immediate reduction of H2S concentration and reduction of oral pathogens in adolescent patients undergoing fixed orthodontic treatment with short-term follow up. Long-term evaluation and complete eradication of halitosis needs to be explored in further clinical trials. In addition, the cost of PDT and its potential side effects should not be disregarded.

Background External stressors such as high altitude and low oxygen are known to affect the human microbiome, and in light of the increased occurrence of dental caries and periodontitis in orthodontic patients, the effect of high altitude and the altered oral environment in orthodontic patients on the oral salivary microbiome was researched. Materials & methods 31 orthodontic patients from high altitude, Aseer region and 25 orthodontic patients, residing at sea level, as controls were included. DNA isolation was done from the saliva collected from the study participants. V3 area of 16s RNA was targeted by universal primers through PCR to decipher the salivary microbiome in both the groups. Results A total of 11 genera belonging to 4 phyla of bacteria were identified in both groups. The most abundant microbiome at the phylum level was: Firmicutes, Bacteroidetes Proteobacteria, and Cyanobacteria. The salivary microbiome was more diverse in sea level controls compared to that of the orthodontic patients at high altitude wherein the presence of only two main phyla: Firmicutes and Proteobacteria were seen. The controls revealed Firmicutes, Proteobacteria, Bacteroidetes and Cyanobacteria. Conclusions The findings of the study suggest that the biodiversity of the salivary microbiome is severely perturbed under the cumulative influences of high altitude and presence of fixed orthodontic appliance. Under these circumstances, a strict and meticulous oral hygiene regimen should be recommended and followed to avoid harmful effects on the periodontal tissues.

Among the social media, Twitter is widely used by political leaders and the public to express opinions about various political issues. These tweets may influence the course of major political events like elections, Brexit and popularity of certain politicians. This common observation led to the research question of this paper: What are the political implications of Twitter postings? To answer this research question, an exploratory qualitative review of tweets was undertaken. Google Scholar was searched using the topic itself as the search term twice with two different time frames till 2019. The search yielded 41 papers. The papers were listed with brief description. A table categorising the methods used in the papers was useful to derive some conclusions. Generally, Twitter can be used for both positive and negative purposes and it can impact either or both the leader and the people who read them. Certain factors are involved in determining the nature of post and its outcomes. Many theories and methods had been used in the papers. Manual, machine learning and automatic analytical tools have been tested and used widely. None of the methods is perfectly suitable for all types of Twitter analysis. General content textual descriptions, characteristics of the texts, symbols, hidden meanings and presentation methods have been used in the tweets examined by the authors. The potential of negative tweets and hate speeches is quite clear. Absence of internal standard definition of these types of posts stands in the way of effective prevention. Some recommendations have been listed based on the findings of this review. A few limitations of this review have also been listed.

The present studies were undertaken to develop solvent-free solid dispersions (SDs) for poorly soluble anti-inflammatory drugs mefenamic acid (MA) and flufenamic acid (FFA) in order to enhance their in vitro dissolution rate and in vivo anti-inflammatory effects. The SDs of MA and FFA were prepared using microwaves irradiation (MW) technique. Different carriers such as Pluronic F127® (PL), Eudragit EPO® (EPO), polyethylene glycol 4000 (PEG 4000) and Gelucire 50/13 (GLU) were used for the preparation of SDs. Prepared MW irradiated SDs were characterized physicochemically using differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infra-red (FT-IR) spectroscopy, powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM). The physicochemical characteristics and drug release profile of SDs were compared with pure drugs. The results of DSC, TGA, FT-IR, PXRD and SEM showed that SDs were successfully prepared. In vitro dissolution rate of MA and FFA was remarkably enhanced by SDs in comparison with pure MA and FFA. The SDs of MA and FFA prepared using PEG 400 showed higher drug release profile in comparison with those prepared using PL, EPO or GLU. The dissolution efficiency for MA-PEG SD and FFA-PEG SD was obtained as 61.40 and 59.18%, respectively. Optimized SDs were also evaluated for in vivo anti-inflammatory effects in male Wistar rats. The results showed significant % inhibition by MA-PEG (87.74% after 4 h) and FFA-PEG SDs (81.76% after 4 h) in comparison with pure MA (68.09% after 4 h) and pure FFA (55.27% after 4 h) (P

Background: One of the popular ancient plants used to treat various diseases such as hyperglycemia, hepatitis, jaundice, bronchitis, and pneumonia has been Juniperus procera (JP). JP is abundantly seen in the region of Al-Baha, Saudi Arabia, and is being used as a medicinal plant traditionally by local healers. Objectives: The objective was to evaluate the anticancer properties of JP from Al-Baha region on SCC-9 cells. Materials and Methods: Colorimetric assays such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, sulforhodamine B assay, and lactate dehydrogenase (LDH) assay were performed to check the cytotoxicity induced and cell viability. Assays evaluating cellular events such as apoptosis and cell cycle were also performed. Results: MTT assay revealed IC50value of 201.6 μg/ml. The cancer cells primed with increasing concentrations of JP displayed an enhanced emission of LDH at elevated concentrations contrasted to cells which were not treated. The samples of JP pooled fraction (PF) (6–9) treated at 160 μg/ml and 320 μg/ml concentration showed 8.39% and 23.37% and 17.35% and 20.89% in early and late phases of apoptosis, respectively. Sample P (PF 6-9) at 160 μg/ml and 320 μg/ml has induced a G2M phase arrest of up to 21.06% and 26.94%. Deoxyribose nucleic acid damage was compared in tested concentrations of sample JP with untreated control cells in SCC9 cells. SCC9 cells that were treated with sample JP PF 6–9 showed the olive moments 23.22 and 37.30 at concentrations 160 μg/ml and 320 μg/ml, respectively. Studies of gene expression showed that increased concentrations of JP triggered the development of caspases and p53. Conclusion: The bioactive compounds found in JP were effective and potent against the SCC-9 cancer cells.

Background/Purpose: Tooth size discrepancy is one of the causative factors of malocclusion. This study aimed to establish the TDS among various malocclusion classes and normal occlusion subjects in a Southern Saudi population. Materials and Methods: The study casts of orthodontic patients from Southern Saudi Arabia (mean age: 19.6 years ± 2.45; n = 120) were randomly selected and grouped into eight equal classes based on Angle's classification and gender. An additional 30 study casts, devoid of malocclusion, having excellent intercuspation and class I occlusion, were grouped into two controls (n = 15) based on gender. The study casts were three-dimensional scanned to measure mesiodistal widths of all the teeth. The calculated anterior ratios (AR) and overall ratio (OR) were statistically analyzed with analysis of variance and t-tests. Results: There were no significant differences in “OR” and “AR” between the genders (P > 0.05) and among the malocclusion and control subgroups (P > 0.05). The mean “OR” (92.01 ± 0.18) and “AR” (78.60 ± 0.27) of the malocclusion group were significantly higher than that of Bolton's ratios (P > 0.05). The “AR” of the control group was significantly higher than Bolton's standards (P = 0.048). However, “OR” was no different (P = 0.105). Malocclusion patients displayed a discrepancy (±2 standard deviation) in “AR” of 22.5% and 'OR' of 6.7% from Bolton's mean (BM). Similarly, the control group displayed a discrepancy in “AR” of 20% and “OR” of 10% from BM. Conclusion: The mean “OR” and “AR” of the Southern Saudi population showed no sexual dimorphism and no significant difference among various malocclusion and control subgroups. The “AR” of the malocclusion and control subgroups did not comply with Bolton's standards.

In recent years there have been a growing number of studies on written corrective feedback (WCF), particularly in terms of the efficacy of different types of WCF. However, few of these studies have investigated what shapes teachers’ WCF practices and how they align with students’ preferences. This study, conducted with staff and students in a large Saudi university that has strict guidelines on WCF provision, examined the teachers’ WCF practices in relation to the institutional guidelines, their own beliefs about the most effective forms of WCF as well as their students’ preferences. Data collected included the feedback given by three teachers on their students’ writing (15 students per teacher), follow-up interviews with the teachers, and questionnaires completed by the students. The study found that although the teachers followed the strict guidelines and provided comprehensive indirect feedback, these practices did not always accord with their beliefs. Most of the WCF given tended to be on mechanics, and the teachers seemed unaware that this was the main focus of their feedback. They were also largely unaware that their students preferred direct feedback and mainly on grammar. We conclude our paper with some policy recommendations.

Aim: To evaluate the effect of varying cellulose casting ring liner length and its prewetting on the marginal adaptation and dimensional accuracy of full veneer metal castings. Materials and Methods: The master die was milled in stainless steel to fabricate the wax pattern. Sixty wax patterns were fabricated with a uniform thickness of 1.5 mm at an occlusal surface and 1 mm axial surface, cervical width at 13.5 mm, and 10 mm cuspal height. The samples were divided into six groups (n = 10). Groups I and II samples had the full-length cellulose prewet and dry ring liner, respectively. The groups III and IV had 2 mm short prewet and dry cellulose ring liner, respectively, whereas groups V and VI were invested in 6 mm short ring liner. The wax patterns were immediately invested in phosphate bonded investment, and casting procedure was completed with nickel-chrome alloy. The castings were cleaned and mean score of measurements at four reference points for marginal adaption, casting height, and cervical width was calculated. The marginal adaption was calculated with Imaje J software, whereas the casting height and cervical width was determined using a digital scale. The data was subjected to one-way analysis of varaince and Tukey post hoc statistical analysis with Statistical Package for the Social Sciences version 20 software. Results: The group II had the best marginal adaption with a gap of 63.786 μm followed by group I (65.185 μm), group IV (87.740 μm), and group III (101.455 μm). A large marginal gap was observed in group V at 188.871 μm. Cuspal height was more accurate with group V (10.428 mm), group VI (10.421 mm), and group II (10.488 mm). The cervical width was approximately similar in group I, group III, and group V. Statistically significant difference was observed in Tukey post hoc analysis between group V and group VI with all the other groups with regards to marginal adaptation. Conclusion: The dry cellulose ring liners provided better marginal adaptation in comparison to prewet cellulose ring liners. Accurate cuspal height was obtained with shorter ring liner in comparison to full-length cellulose ring liners.

One of the most feared complications after orthodontic treatment is the external root resorption. Root resorption leads to shortening of root and thus, the integrity of teeth arch is broken down. Both the cervical and apex zones of the roots can be affected by it due to orthodontic forces involved in the tooth movement. The future of the teeth involved may also be compromised due to orthodontic treatment. It is necessary that the patient is pre-informed about the root resorption’s risks as a result of orthodontic treatment. It is possible to detect the orthodontic-induced root resorption both during the active phase of treatment and after the orthodontic treatment. The degree and severity of orthodontic-induced root resorption are dependent on a number of factors involving environmental factors and the host himself. The paper talks about the root resorption, its degree of severity, mechanism, detection methods, and significant correlation with individual susceptibility, tooth structure, fixed appliance treatment, orthodontic tooth movement, orthodontic force, and treatment duration.

This cross-sectional study was done to evaluate linear and angular measurement of lip morphology in Saudi adults and their comparison with the norms. After taking their history and physical examination, the patients were referred for a lateral Cephalograph using Dolphin Imaging Software. Linear and angular measurements related to lip morphology were recorded. Significant differences were found in the lower lip to E line, upper and lower lip protrusion, upper and lower lip to S line and upper and lower lip thickness, nasolabial angle, Z angle, with Saudi males having more prominent, thicker lips than the Saudi females. Comparison with the norms revealed that both lips are prominent among Saudi males, while in females there is an increased prominence of the lower lip. Value of the lower lip to H line is increased in both genders, males have prominent and thicker upper lips compared to the females and the lower lip in both genders is more prominent compared to the norms.

Aim: The aim of this study was to assess the oral health knowledge, behavior, and practices related to use of miswak (chewing stick) in population of Aseer Region, Saudi Arabia. Subjects and Methods: Of the 2023 participants, 1666 (83.3%) were females and 334 (16.7%) were males. The questionnaires having 10 online questions were used to assess the knowledge of oral hygiene methods, including frequency, reason, and methods for miswak use. Statistical Analysis Used: The survey data were collected and organized into Microsoft Excel spreadsheets (Microsoft Inc., USA) and were statistically analyzed utilizing the Statistical Package for the Social Sciences version 20.0 software (IBM Inc., USA). The statistical test used here was the Chi-square test, and P < 0.05 was considered to be statistically significant (P < 0.05). Results: Participants responded regarding the primary oral hygiene methods; 46.5% respondents used toothbrushes, 44.5% used both toothbrushing and miswak, and 8% used only miswak as their primary tooth cleaning method. 28.2% respondents cleaned their teeth with tooth brush or miswak at least once a day, 37.6% twice, 28.4% whenever required, and 5.9% infrequently. Majority of the participants, i.e. 70.2% were using miswak with taper and as a brush to clean all tooth surfaces. About 84.7% feel fresh, and teeth are whiter after the use of miswak. Nearly, 84.7% prefer to continue using miswak in combination with other teeth cleaning methods, which may have more benefits. Conclusions: In our study, most common type of oral hygiene method employed is toothbrush and in combination with miswak. Chewing stick use was common among participants with religious advice being the dominant reason for usage.