1. Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters
- Author
-
S.V. Albers, Marieke G. L. Elferink, Arnold J. M. Driessen, Wil N. Konings, GBB Cluster Microbiologie, Moleculaire Microbiologie, and Groningen Biomolecular Sciences and Biotechnology
- Subjects
Signal peptide ,Glycosylation ,Archaeal Proteins ,Molecular Sequence Data ,ved/biology.organism_classification_rank.species ,HALOFERAX-VOLCANII ,ATP-binding cassette transporter ,Cellobiose ,Biology ,Microbiology ,Sulfolobus ,CLONING ,chemistry.chemical_compound ,Operon ,Concanavalin A ,MOLECULAR CHARACTERIZATION ,Amino Acid Sequence ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,Molecular Biology ,Sequence Homology, Amino Acid ,ved/biology ,Binding protein ,Cell Membrane ,Sulfolobus solfataricus ,FLAGELLINS ,Glucose transporter ,GENOME SEQUENCE ,Biological Transport ,GENE ,Trehalose ,TREHALOSE-PRODUCING ENZYMES ,Glucose ,chemistry ,Biochemistry ,THERMOTOGA-MARITIMA ,BACTERIA ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Carbohydrate Metabolism ,ATP-Binding Cassette Transporters ,ARCHAEON THERMOCOCCUS-LITORALIS ,Energy source ,Mannose - Abstract
The extreme thermoacidophilic archaeon Sulfolobus solfataricus grows optimally at 80 degrees C and pH 3 and uses a variety of sugars as sole carbon and energy source. Glucose transport in this organism is mediated by a high-affinity binding protein-dependent ATP-binding cassette (ABC) transporter. Sugar-binding studies revealed the presence of four additional membrane-bound binding proteins for arabinose, cellobiose, maltose and trehalose. These glycosylated binding proteins are subunits of ABC transporters that fall into two distinct groups: (i) monosaccharide transporters that are homologous to the sugar transport family containing a single ATPase and a periplasmic-binding protein that is processed at an unusual site at its amino-terminus; (ii) di- and oligosaccharide transporters, which are homologous to the family of oligo/dipeptide transporters that contain two different ATPases, and a binding protein that is synthesized with a typical bacterial signal sequence. The latter family has not been implicated in sugar transport before. These data indicate that binding protein-dependent transport is the predominant mechanism of transport for sugars in S. solfataricus.
- Published
- 2001
- Full Text
- View/download PDF