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The objective of this work was to evaluate air layering, cutting, and grafting, with different indolebutyric acid (IBA) rates, for the vegetative propagation of Eugenia involucrata. Air layering was carried out for two consecutive years with five different IBA rates. Cutting was tested for woody and herbaceous cuttings, using four IBA rates, in three seasons. The grafting methods – cleft graft and splice graft – were tested in two seasons. For the air layering, cutting, and grafting trials the experimental designs were randomized complete blocks, completely randomized in a 3x4 factorial arrangement, and completely randomized, respectively. Plants were evaluated for rooting, survival percentage, and shoot development. In air layering, low rooting rates of 0 to 50% were observed, the use of IBA showed no consistent results, and no resulting propagules survived after being transplanted. The cutting technique showed low survival, rooting, and leaf retention rates, with no consistent effect of IBA rates. Grafting showed 35 to 50% graft fixation, with no significant difference between the splice and cleft grafting techniques. The air layering and cutting techniques are not efficient for the propagation of E. involucrata, as they do not favor the survival and rooting of seedlings. Grafting, by both techniques, is the most efficient method for E. involucrata propagation. Resumo O objetivo deste trabalho foi avaliar a alporquia, a estaquia e a enxertia, com diferentes doses de ácido indolbutírico (AIB), para a propagação vegetativa de Eugenia involucrata. A alporquia foi realizada por dois anos consecutivos, com cinco diferentes doses de AIB. A estaquia foi testada com estacas lenhosas e herbáceas, quatro doses de AIB, em três estações do ano. Os métodos de enxertia – garfagem em fenda cheia e inglês simples – foram avaliados em duas estações do ano. Para os ensaios de alporquia, estaquia e enxertia, os delineamentos experimentais foram em blocos ao acaso, inteiramente casualizado em arranjo fatorial 3x4 e inteiramente casualizado, respectivamente. As plantas foram avaliadas quanto ao enraizamento, à percentagem de sobrevivência e ao desenvolvimento da parte aérea. Na alporquia, foram observadas baixas taxas de enraizamento foram observadas na alporquia de 0 a 50%, o uso de AIB não apresentou resultados consistentes e nenhum propágulo resultante sobreviveu após o transplante. A técnica de estaquia mostrou baixas taxas de sobrevivência, enraizamento e retenção foliar, sem efeito consistente das doses de AIB. A enxertia mostrou de 35 a 50% de fixação do enxerto, sem diferença significativa entre as técnicas de enxerto de emenda e de fenda. As técnicas de alporquia e estaquia não são eficientes para a propagação de E. involucrata, pois não favorecem a sobrevivência e o enraizamento das mudas. A enxertia, por ambos os métodos, é a técnica mais eficiente para a propagação de E. involucrata.

Tree peony (Paeonia suffruticosa Andr.), a unique traditional flower in China, is famous for its ornamental value, medical use, and edible oil production. Traditional propagation methods, such as sowing, dividing, and grafting do not allow the large-scale production of selected peony varieties. Therefore, the objective of our study is to evaluate an air-layering technique on the rooting success of three tree peony cultivars (‘Baoqing Hong’, ‘Quehao’, and ‘Xishi’). The experiments were established through consideration of the influence of the time of the year the rooting was performed (mid-May, mid-June, or mid-July) and the growth regulators (1-naphthaleneacetic acid-NAA and indole-3-butyric acid-IBA) applied at different concentrations (1000 mg/L, 1500 mg/L, 2000 mg/L). The results showed that the rooting rate was the highest when the air-layering time occurred in mid-June, and the rooting rate of ‘Quehao’ was found to be the most significant, reaching 100%. The rooting percentages of 82.86% and 77.14% were obtained for ‘Baoqing Hong’ and ‘Xishi’, respectively. The growth regulators affected the rooting performance of the three cultivars differently. The rooting parameters of ‘Quehao’ were negatively correlated with the concentration of NAA but positively correlated with IBA, whereas the ‘Baoqing Hong’ and ‘Xishi’ cultivars showed no dose dependence for the supplied growth regulators. Root number, root tip number, and maximum root length in ‘Quehao’ were higher than those of the other two cultivars. The conclusion of our study is that the air-layering technique is a suitable method for achieving satisfactory propagation of selected tree peony cultivars.

Due to over exploitation, the population of Eusideroxylon zwageri is decreasing drastically. One simple technique to conserve the last remaining genetic resources of E. zwageri is by air layering. The experiment was divided into two. The experiment was conducted from July 2018 to February 2019. The first experiment was conducted using branches that grew from coppices while the second experiment using twigs that grew from normal adult trees. Randomized Block Design was applied with three replicates. The treatments were consisted of nine concentration of auxin namely 0 ppm of auxin; 1, 000 ppm; 1,500 ppm; 2,000 ppm and 2,500 ppm of IAA and 1,000 ppm; 2,000 ppm; 3,000 ppm and 4,000 ppm of IBA. The result of experiment shows that the IBA growth regulator seems to be more effective in regulating growth and rooting of E. zwageri air layering compared to IAA. Results for air layering which was taken from coppices revealed that 1,000 ppm of IBA obtained the best results. Air layering of branches taken from coppice is possible even without any additional growth regulator. While, air layering of twigs which was taken from normal trees revealed that the best treatment is 3,000 ppm of IBA.

The Myrtaceae family has representatives spread worldwide, and Jabuticaba tree is an important species native to Brazil, which is grown not only in the backyards and domestic orchards, but also in commercial orchards, landscaping projects and in several tropical and subtropical countries. The propagation of this species in a sexual form or by seeds is simple and efficient, being able to reach 100%. However, long juvenile phase and genetic segregation have led to the search for methods that allow the propagation of selected trees and guarantee through their standardized and productive clones, fruit quality and fruiting precocity. Protocols for asexual propagation are still scarce, but they show efficient methods, making possible the commercial use and valorization of this Brazilian fruit tree. Grafting methods have proven to be efficient in more than 70% of cases. Herbaceous cutting in Jabuticaba trees shows rooting around 10%. Air layering shows rooting rates above 80%. Other methods considered for domestic use have shown satisfactory results in the formation of young saplings through woody cuttings. New studies on micropropagation and better control of herbaceous stem cutting processes could stimulate and economically increase the propagation of this fruit tree for commercial crops to produce fruits for fresh consumption or processing. Air layering and grafting are the most currently indicated methods for propagation of jabuticaba tree. This review paper shows the results of other papers and their protocols for obtaining jabuticaba tree seedlings and clonal plants. Resumo Dentre a família Myrtaceae com representantes espalhados por todo o planeta, a jabuticabeira é uma importante espécie nativa do Brasil e que passou a ser apreciada não só nos limites de quintais e pomares domésticos, mas já pode ser encontrada em pomares comercias, projetos de paisagismo e em vários países com clima tropical e subtropical. A propagação desta espécie de forma sexuada ou via sementes é bastante simples e eficiente, podendo chegar a 100%. Porém, o longo período de juvenilidade e a segregação têm levado à busca por métodos que permitam propagar matrizes selecionadas e garantir, em seus clones, plantas padronizadas e produtivas, a qualidade dos frutos e a precocidade na frutificação. Os protocolos para propagação assexuada ainda são escassos, mas mostram métodos eficientes, viabilizando a exploração comercial e a valorização desta frutífera brasileira. O método de enxertia em garfagem têm-se mostrado eficiente em mais de 70% dos casos. A estaquia herbácea mostra enraizamento em torno de 10%. A alporquia com índices de enraizamento acima de 80%. Outros métodos considerados de uso doméstico têm mostrado resultados satisfatórios na formação de mudas de jabuticabeira através de estacas lenhosas. Novos estudos, como a micropropagação e melhor controle nos processos de estaquia herbácea, devem estimular e viabilizar economicamente a multiplicação desta frutífera para cultivos comerciais, seja para produção de frutos frescos, seja para processamento. A alporquia e a enxertia em garfagem são os métodos mais indicados atualmente para propagação de jabuticabeira. Neste artigo de revisão constam resultados de trabalhos e protocolos para obtenção de mudas de jabuticabeira.

The different propagation systems for litchis are reviewed. After examining the main morphological and developmental characteristics of the plant and the description of the structures and materials, including types of substrate required for the propagation of this species, the different methods for its propagation are discussed. Propagation by seeds is only used for breeding or research purposes or for raising rootstock for grafting. Although propagation by cuttings is done in many countries and stooling has been reported as highly successful in India, air layering is the most common commercial way of propagation for this species. Despite that the cambium activity varies from place to place around the stem with only 30% of the cambium being active at any one time grafting is of current use in China. Resumo Foram estudados os diferentes tipos de propagação da lichieira. Depois de examinar as principais características morfológicas e de desenvolvimento da planta e de uma descrição das estruturas de propagação, materiais e tipos de substrato necessários para esta espécie, descrevem-se os diferentes métodos de propagação desta frutifera. A propagação por semente usa-se apenas para melhoramento, pesquisa ou obtenção de porta-enxertos. Embora a propagação por estaquia seja usada em vários países e que a propagação seja por mergulhia é praticada na Índia com grande sucesso, a alporquia é o sistema de propagação comercial mais usado no mundo para esta espécie. Ainda que a atividade do meristema varie em torno do caule, e apenas 30% do mesmo esteja ativo, a enxertia é prática comum na China.

Nursery trees of stone fruits (Prunus spp.) are traditionally produced by union of two distinct genotypes - the rootstock and the scion - which, by grafting, form a composite plant that will be maintained throughout of all plant life. In Brazil, the rootstocks are predominantly seed propagated and therefore usually results in heterogeneous trees for vigor and edaphic adaptation. However, with advances in rootstock breeding programs that released cultivars and certification in several countries (notably in Europe), the system will come gradually evolving for vegetative propagation (cuttings and tissue culture) and use of seeds of selected rootstocks with specific characteristics and potted nursery trees production. For scion cultivar propagation, the budding system (with its many variations) has predominantly been adopted in major producing countries. This review had as objective to comment main propagation methods adopted for rootstocks and scion in peach, nectarine and plum, and recent technical progress obtained as well as the needs of improvement for nursery tree production. RESUMO Mudas de frutíferas de caroço (Prunus spp.) são tradicionalmente produzidas pela união de dois genótipos distintos - o porta-enxerto e a cultivar copa - que, por meio da enxertia, formam uma planta composta. No Brasil, os porta-enxertos são predominantemente obtidos de sementes, normalmente resultando em plantas com heterogeneidade de suas características. Com os avanços nos programas de melhoramento genético de porta-enxertos, lançamentos de cultivares e programas de certificação de mudas em diversos países (notadamente na Europa), o sistema vêm gradativamente evoluindo para a propagação vegetativa (estaquia e cultura de tecidos), uso de sementes de genótipos selecionados com características específicas e produção de mudas em embalagens. Para a propagação das cultivares copa, a enxertia (com suas diversas variações) têm sido predominantemente adotada nos principais países produtores. O objetivo desta revisão bibliográfica é abordar os principais métodos de propagação adotados para porta-enxertos e para copas em pessegueiro, nectarineira e ameixeira, e os avanços técnicos obtidos, bem como as necessidades de melhorias para a produção de mudas de qualidade.

The production of quality seedlings is important for the crop cycle and the main propagation type is by the air layering technique, which can be enhanced by using different substrates and plant growth regulators. Therefore, the objective of this study was to evaluate the use of a biostimulant and substrate types in the propagation of litchi tree by air layering. The experiment was installed in a commercial area in the city of Mogi Mirim, SP, Brazil and conducted in a randomized block design with a 5x2 factorial with four replications. The treatments consisted of five Stimulate® biostimulant concentrations (0, 3, 6, 9, and 12 ml L-1) and two substrate types (Plantmax® and earthworm humus). After 120 days, the root length (cm), number of roots, calloused and rooted layers percentage (%), root fresh and dry mass (g) and root volume (cm3) were evaluated. Based on the results, it was found that the concentrations of 6.1 and 6.9 ml L-1 promoted greater success in litchi tree propagation and that the layers can be produced on both evaluated substrates.