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El artículo analiza el trabajo de las amas de leche en Montevideo entre 1852 y 1890. Ubica el tema en el marco de las transformaciones ocurridas en el mercado de trabajo urbano de la ciudad durante el proceso de transición de la economía uruguaya al capitalismo en el contexto de la primera globalización. Mediante una variedad de fuentes (avisos de trabajo en la prensa, documentación del asilo, manuales médicos, documentos de las autoridades municipales) estudia la inserción de las amas de leche en el ámbito privado de las familias y su rol como trabajadoras del Asilo de expósitos y huérfanos de la ciudad. Da cuenta de la presencia de mujeres negras trabajando como amas en la inmediata post abolición y de la creciente participación de europeas en las décadas posteriores a 1860. El trabajo precario que realizaban resultaba fundamental para la subsistencia de sus familias y formaba parte de una de las escasas alternativas laborales que tenían las mujeres (y madres) pobres de la ciudad.

Image restoration using deep learning attempts to create an image recovery system that can restore outdated and corrupted images regardless of the type of noise present. Photos play an important role in capturing and preserving cherished moments in today's digital age. However, due to a variety of environmental conditions, these images can get distorted over time. Manual recovery is time-consuming and labor-intensive, emphasizing the need for an automated alternative. Deep learning techniques, notably convolutional neural networks, are used in the proposed system, which has demonstrated promising results in image processing tasks. The essay goes over these approaches in detail, focusing on image noise reduction, deblurring, dehazing, and super-resolution. Different network topologies are investigated, including those with residual or merge-skip connections, as well as their receptive fields and the usage of unsupervised autoencoder processes. The study also looks at image quality criteria to see how helpful they are in image recovery. An effective deblurring network and adaptable training algorithms for highresolution recovery tasks are suggested to handle the special difficulty of deblurring. The proposed method's performance is compared to state-of-the-art approaches utilizing both quantitative and qualitative assessments. The study finishes with a discussion of prospective future research topics and outstanding challenges in image recovery. The ultimate goal of this research is to create a robust and efficient image recovery system capable of restoring photos to their original quality regardless of the type or severity of corruption or noise present.

This рrоjeсt рresents reseаrсh оn eleсtriсity рrоduсtiоn using а соmbinаtiоn оf thermоeleсtriс generаtоrs аnd thermоeleсtriсity. Mоst оf the heаt energy in industry is dissiраted аs wаste heаt tо the envirоnment. This exсess heаt саn be reused tо рrоduсe eleсtriсity. The рrоblems аssосiаted with glоbаl wаrming аnd the dwindling suррly оf fоssil fuels hаve mаde imрrоving the effiсienсy оf аny industriаl рrосess а рriоrity. Оne wаy tо imрrоve effiсienсy is tо develор methоds thаt utilize wаste heаt is оften wаsted. Twо рrоmising teсhnоlоgies thаt were fоund tо be useful fоr this рurроse were thermоeleсtriс generаtоrs аnd heаt рiрes. Therefоre, this рrоjeсt invоlved mаking а benсh tyрe, рrооf оf соnсeрt mоdel оf роwer рrоduсtiоn by thermоeleсtriс generаtоrs using heаt рiрes аnd simulаted hоt аir. Keywords: Waste heat, waste heat Recovery, TEGs, Temperature Measurement, Voltage Measurement, Electricity etc.

The drive of this study was to examine and investigate ARDS in paediatrics. Using the patient data during diagnosis among all representing relatively small percentages of total admission the most difficult patient population for a clinical to manage is ARDS. We present a 2-year-1-month-old female child with pneumonitis who developed ARDS. ARDS in pediatrics palicc suggested that for mild to severe pARDS, the consensus conference recommended "should be considered" after the peep has been optimized. Aching in the abdomen (in pancreatitis) coughing (often with white or pink frothy mucus) fatigue, fever, and shortness of breath are entirely indications of asthma. Tracheal intubation and mechanical ventilation are obligatory to advance the ailment of a patient with ARDS. Acute respiratory distress syndrome (ARDS) is prevalent in ventilated patient’s youngsters and is present in up to 30 percent of the overall of all paediatric intensive care unit casualties. Regardless of the circumstance that ARDS is diagnosed via medical criteria, inherited markers of acute lung injury have been comprehensively sightseen in adults and children. Anomalies of the flow of fluids in the body such as inflammatory markers, alveolar epithelial disruption, and high coagulation, along with other anomalous findings in the circulation such as bronchoalveolar lavage, have enriched our indulgent of ARDS pathologic variations.

The Golgi complex has long been recognised as an important homeostasis hub, where a multitude of signalling pathways and essential cellular processes intersect. Yet its communication with the cell nucleus remains largely unexplored. To this end, we have analysed genome-scale localisation data of the Human Protein Atlas which revealed an unexpected high number of Golgi and nuclear dual-localisation proteins and several pathways including surprising DNA repair. Amongst these proteins we found RAD51C, a regulatory Homologous Recombination (HR) repair protein, that localises to the Golgi and in response to double-strand DNA breaks, the Golgi protein population of RAD51C redistributes to form DNA repair foci. Depletion of the Golgin Giantin induces the redistribution of the RAD51C Golgi pool to form nuclear foci, independent of DNA damage induction. Concurrent with a significant increase in genomic instability and inhibition of HR signalling regulators. Altogether, we present evidence for a novel pathway where the Golgi is a central regulatory hub for HR-mediated DNA repair and potentially other repair pathways.

This paper represents the design and simulation of a wireless patient health monitoring system. Generally, in the hospitals where patient’s body temperature needs to be continuously monitored, which is usually done by a doctor or other paramedical staff by continuously observing the temperature, pulse rate, blood pressure and maintaining its record. This is a very monotonous routine and can really be nerve-racking, especially in overcrowded hospitals. The primary function of this system is to monitor the temperature, blood pressure and pulse rate of a patient’s body, and transmit the information wirelessly to the doctor’s office on the LCD display unit. In this advanced system, a transmitting unit constantly scan patient’s body temperature, blood pressure and pulse rate through digital sensors, and shows them on the transmitter LCD. At the receiving end, a receiver is used to collect the data, decode it and feed them to another microcontroller which then displays it on the doctor’s LCD screen. The receiver unit is kept in the doctor’s office to constantly show the patient’s body temperature, blood pressure and pulse rate wirelessly. An alarm is also activated at the receiver end where the doctor will be, and is activated when the patient’s temperature, blood pressure and pulse rate goes below or above the normal human threshold value, which are 37°C, SBP- less than 120 mm Hg, DBP- less than 80mm Hg and 70 beats per minute. An SMS will be sent to the doctor, just in case he or she is outside the area and to present as a path for continuous update. The advanced system was simulated using Proteus software and programs written in Embedded C language. The result achieved shows a systematic method of relating information to the doctor on duty for urgent attention to patients.

The present study entails the phytoremediation potential of different bamboo species on 5-year-old FA-dumped site near Koradi thermal power plant of Nagpur, Maharashtra, India. The selected FA-dumped site was treated with farmyard manure, press mud, and bio fertilizer followed by plantation of six promising species of bamboo namely Bambusa balcooa Roxb., Dendrocalamus stocksii (Munro.) M. Kumar, Remesh and Unnikrishnan, Bambusa bambos (L.) Voss, Bambusa wamin E.G. Camus, Bambusa vulgaris var. striata (Lodd. ex Lindl.) Gamble, and Bambusa vulgaris var. vittata Riviere and Riviere. The experimental results indicated that the organic input in the FA-dumped site nourished the soil by improving its physico-chemical, and biological characteristics. The results revealed the contamination of the site with different trace elements in varied quantity including Cr (89.29 mg kg−1), Zn (84.77 mg kg−1), Ni (28.84 mg kg−1), Cu (22.91 mg kg−1), Li (19.65 mg kg−1), Pb (13.47 mg kg−1), and Cd (2.35 mg kg−1). A drastic reduction in concentration of heavy metals in FA was observed after 1 year of bamboo plantation as compared to the initial condition. The results showed that bamboo species are good excluders of Ba, Co, Cr, Li, Ni, Mn, and Zn, whereas they are good accumulators of Cd, Pb, and Cu. The values of biochemical parameters, such as pH, total chlorophyll, ascorbic acid (AA), and relative water content of all the bamboo leaves ranged from 5.11–5.70, 1.56-6.33 mg g−1, 0.16-0.19 mg g−1, and 60.23–76.68%, respectively. It is thereby concluded that the bamboo plantation with biofertilizers and organic amendments may indicate adaptive response to environmental pollution on FA-dumped site.

The cell cycle, over which cells grow and divide, is a fundamental process of life. Its dysregulation has devastating consequences, including cancer1-3. The cell cycle is driven by precise regulation of proteins in time and space, which creates variability between individual proliferating cells. To our knowledge, no systematic investigations of such cell-to-cell proteomic variability exist. Here we present a comprehensive, spatiotemporal map of human proteomic heterogeneity by integrating proteomics at subcellular resolution with single-cell transcriptomics and precise temporal measurements of individual cells in the cell cycle. We show that around one-fifth of the human proteome displays cell-to-cell variability, identify hundreds of proteins with previously unknown associations with mitosis and the cell cycle, and provide evidence that several of these proteins have oncogenic functions. Our results show that cell cycle progression explains less than half of all cell-to-cell variability, and that most cycling proteins are regulated post-translationally, rather than by transcriptomic cycling. These proteins are disproportionately phosphorylated by kinases that regulate cell fate, whereas non-cycling proteins that vary between cells are more likely to be modified by kinases that regulate metabolism. This spatially resolved proteomic map of the cell cycle is integrated into the Human Protein Atlas and will serve as a resource for accelerating molecular studies of the human cell cycle and cell proliferation.

The understanding of how spatio-temporal patterns of neural activity may arise in the cortex of the brain has advanced with the development and analysis of neural field models. Replicating this suc...

In this work, the influences on the torque generated by permanent magnet synchronous machines are identified to provide approaches to improve the accuracy of torque calculation. By analyzing the torque generation mechanism, basic machine parameters influencing the generated torque are identified. The machine torque is split into different components, i.e. synchronous, reluctance, and parasitic torque components. The parasitic component results from non-linearities such as saturation. In a second step, environmental and operational conditions influencing those basic parameters are determined. Based on this analysis, the remanence flux density of the permanent magnets, the magnetization behavior of the soft-magnetic material, and the iron losses are selected for the study. Two traction drive machine designs are used in the parameter study. The machine torque is evaluated by performing 2D magnetostatic Finite-Element simulations of the investigated machine designs. The results show quantify the interdependencies between the torque components and the model parameters. The significance of the influence of the parameters on the accuracy of the torque calculation is discussed.

Supplemental Digital Content is available in the text., Background: Aortic valve stenosis (AVS), which is the most common valvular heart disease, causes a progressive narrowing of the aortic valve as a consequence of thickening and calcification of the aortic valve leaflets. The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) in cardiovascular prevention have recently been demonstrated in a large randomized, controlled trial. In addition, n-3 PUFAs serve as the substrate for the synthesis of specialized proresolving mediators, which are known by their potent beneficial anti-inflammatory, proresolving, and tissue-modifying properties in cardiovascular disease. However, the effects of n-3 PUFA and specialized proresolving mediators on AVS have not yet been determined. The aim of this study was to identify the role of n-3 PUFA–derived specialized proresolving mediators in relation to the development of AVS. Methods: Lipidomic and transcriptomic analyses were performed in human tricuspid aortic valves. Apoe−/− mice and wire injury in C57BL/6J mice were used as models for mechanistic studies. Results: We found that n-3 PUFA incorporation into human stenotic aortic valves was higher in noncalcified regions compared with calcified regions. Liquid chromatography tandem mass spectrometry–based lipid mediator lipidomics identified that the n-3 PUFA–derived specialized proresolving mediator resolvin E1 was dysregulated in calcified regions and acted as a calcification inhibitor. Apoe−/− mice expressing the Caenorhabditis elegans Fat-1 transgene (Fat-1tg×Apoe−/−), which enables the endogenous synthesis of n-3 PUFA and increased valvular n-3 PUFA content, exhibited reduced valve calcification, lower aortic valve leaflet area, increased M2 macrophage polarization, and improved echocardiographic parameters. Finally, abrogation of the resolvin E1 receptor ChemR23 enhanced disease progression, and the beneficial effects of Fat-1tg were abolished in the absence of ChemR23. Conclusions: n-3 PUFA-derived resolvin E1 and its receptor ChemR23 emerge as a key axis in the inhibition of AVS progression and may represent a novel potential therapeutic opportunity to be evaluated in patients with AVS.

The causes of chronic pelvic pain (CPP) in women can be multifactorial and significantly affect quality of life. Finding the cause(s) for CPP often takes a multidisciplinary approach. One commonly overlooked cause of CPP is pelvic venous disease (PVD). The causal relationship between PVD and CPP has been well-documented in the literature, but with limited assessment or treatment guidelines to advise health care providers, there continues to be a limited understanding of PVD. This article aims to ensure nurse practitioners are well equipped to recognize women presenting with symptoms suggestive of PVD and expedite referral when needed for further evaluation.

The number of potential pediatric heart transplant recipients continues to exceed the number of donors, and consequently the waitlist mortality remains significant. Despite this, around 40% of all donated organs are not used and are discarded. This document (62 authors from 53 institutions in 17 countries) evaluates factors responsible for discarding donor hearts and makes recommendations regarding donor heart acceptance. The aim of this statement is to ensure that no usable donor heart is discarded, waitlist mortality is reduced, and post-transplant survival is not adversely impacted.

A great deal of understanding can be gleaned from direct observation of organismal growth, development, and behavior. However, direct observation can be time consuming and influence the organism through unintentional stimuli. Additionally, video capturing equipment can often be prohibitively expensive, difficult to modify to one’s specific needs, and may come with unnecessary features. Here, we describe the PiSpy, a low-cost, automated video acquisition platform that uses a Raspberry Pi computer and camera to record video or images at specified time intervals or when externally triggered. All settings and controls, such as programmable light cycling, are accessible to users with no programming experience through an easy-to-use graphical user interface. Importantly, the entire PiSpy system can be assembled for less than $100 using laser-cut and 3D-printed components. We demonstrate the broad applications and flexibility of the PiSpy across a range of model and non-model organisms. Designs, instructions, and code can be accessed through an online repository, where a global community of PiSpy users can also contribute their own unique customizations and help grow the community of open-source research solutions.

Studying the electromagnetic behavior of gearless wind turbines, related to parasitic excitation of vibrations and audible noise emission, comes to the fore of science. Reliable simulation and testing methods are essential in this context. The electrical drive train, consisting of the generator that is coupled to a full-scale converter in modern direct-driven turbines, is the first component in the electrical energy supply chain. Particularly, the modeling of its interaction is focused on to study the harmonic content of the generator currents and excited forces in the air gap. Therefore, a weak numerical field-coupling method is used in this contribution to consider both, the current waveform resulting from the coupling and the magnetic forces computed by Finite Element Analyses (FEA). Measurement data, that is collected by means of a 4MW full-scale test rig, is considered to motivate and validate the proposed model approach. Moreover, the anomalous electromagnetic design of the considered generator is studied by the field-circuit simulation and corresponding magnetic air gap force harmonics are analyzed.

Prenatal prediction of postnatal univentricular versus biventricular circulation in patients with borderline left ventricle (bLV) remains challenging. This study investigated prenatal fetal echocardiographic parameters and postnatal outcome of patients with a prenatally diagnosed bLV. We report a retrospective study of bLV patients at four prenatal centers with a follow-up of one year. BLV was defined as z-scores of the left ventricle (LV) between -2 and -4. Single-ventricle palliation (SVP), biventricular repair (BVR), and no surgical or catheter-based intervention served as the dependent outcome. Prenatal ultrasound parameters were used as independent variables. Cut-off values from receiver operating characteristic curves (ROC) were determined for significant discrimination between outcomes. A total of 54 patients were diagnosed with bLV from 2010 to 2018. All were live births. Out of the entire cohort, 8 (15 %) received SVP, 34 (63 %) BVR, and 12 (22 %) no intervention. There was no significant difference with regard to genetic or extracardiac anomalies. There were significantly more patients with endocardial fibroelastosis (EFE) in the SVP group compared to the BVR group (80 % vs. 10 %), (p 0.001). Apex-forming LV (100 % vs. 70 %) and lack of retrograde arch flow (20 % vs. 80 %) were associated with no intervention (p 0.001). With respect to BVR vs. SVP, the LV sphericity index provided the highest specificity (91.7 %) using a cutoff value of ≤ 0.5. The majority of bLV patients maintained biventricular circulation. EFE, retrograde arch flow, and LV sphericity can be helpful parameters for counseling parents and further prospective studies can be developed.ZIEL: Die pränatale Vorhersage eines postnatal univentrikulären versus biventrikulären Kreislaufs bei Föten mit grenzwertigem linkem Ventrikel (bLV, “borderline left ventricle”) bleibt eine Herausforderung. Diese Studie untersuchte pränatale fetale echokardiografische Parameter und den postnatalen Outcome von Patienten mit einer pränatal diagnostizierten bLV. Wir berichten über eine retrospektive Studie von Föten mit bLV an 4 Pränatalzentren mit einer Nachverfolgung von einem Jahr. BLV wurde definiert als z-Scores des linken Ventrikels (LV) zwischen –2 und –4. Als abhängiger Outcome dienten die Einzelventrikel-Palliation (SVP), die biventrikuläre Korrektur (BVR) und keine chirurgische oder Katheter-basierte Intervention. Die pränatalen Ultraschallparameter wurden als unabhängige Variablen verwendet. Die Cut-off-Werte aus den Receiver-Operating-Characteristic (ROC) -Kurven wurden für eine signifikante Diskriminierung zwischen den Outcomes ermittelt. Von 2010–2018 wurden insgesamt 54 Patienten mit bLV diagnostiziert. Alle waren Lebendgeburten. Von der gesamten Kohorte erhielten 8 (15 %) SVP, 34 (63 %) BVR und 12 (22 %) keine Intervention. Es gab keinen signifikanten Unterschied in Bezug auf genetische oder extrakardiale Anomalien. Es gab signifikant mehr Patienten mit endokardialer Fibroelastose (EFE) in der SVP- im Vergleich zu BVR-Gruppe (80 % vs. 10 %; p 0,001). Ein an der Herzspitzenbildung beteiligter LV (100 % vs. 70 %) und fehlender retrograder Bogenfluss (20 % vs. 80 %) waren mit keiner Intervention assoziiert (p 0,001). Für die Differenzierung von BVR vs. SVP lieferte der LV-Sphärizitätsindex bei einem Cut-off-Wert von ≤ 0,5 die höchste Spezifität (91,7 %). Die Mehrheit der bLV-Patienten hielt einen biventrikulären Kreislauf aufrecht. EFE, retrograder Bogenfluss und LV-Sphärizität können hilfreiche Parameter bei der Beratung der Eltern und der Entwicklung weiterer prospektiver Studien sein.

Cardiac alternans, in which the membrane potential and the intracellular calcium concentration exhibit alternating durations and peak amplitudes at consecutive beats, constitute a precursor to fatal cardiac arrhythmia such as sudden cardiac death. A crucial question therefore concerns the onset of cardiac alternans. Typically, alternans are only reported when they are fully developed. Here, we present a modelling approach to explore recently discovered microscopic alternans, which represent one of the earliest manifestations of cardiac alternans. In this case, the regular periodic dynamics of the local intracellular calcium concentration is already unstable, while the whole-cell behaviour suggests a healthy cell state. In particular, we use our model to investigate the impact of calcium diffusion in both the cytosol and the sarcoplasmic reticulum on the formation of microscopic calcium alternans. We find that for dominant cytosolic coupling, calcium alternans emerge via the traditional period doubling bifurcation. In contrast, dominant luminal coupling leads to a novel route to calcium alternans through a saddle-node bifurcation at the network level. Combining semi-analytical and computational approaches, we compute areas of stability in parameter space and find that as we cross from stable to unstable regions, the emergent patterns of the intracellular calcium concentration change abruptly in a fashion that is highly dependent upon position along the stability boundary. Our results demonstrate that microscopic calcium alternans may possess a much richer dynamical repertoire than previously thought and further strengthen the role of luminal calcium in shaping cardiac calcium dynamics.

Biology, tolerance, and metal (Pb and Cr) accumulating ability of Gomphrena celosoides were studied under hydroponic conditions. The seedlings were raised in Hoagland’s solution containing ...

La consolidación de una sociedad capitalista a fines del siglo XIX y la llegada de inmigrantes europeos con militancia de izquierda se presentan habitualmente como los factores clave para comprender el origen del movimiento obrero argentino y uruguayo, en los que las primeras huelgas se habrían iniciado a partir de 1878. Todo esto tiene una importancia indudable, pero encuadra esta historia en el “tiempo corto”. ¿Alcanza con él para explicar las prácticas del movimiento obrero emergente? Este artículo plantea la conveniencia de rastrear las formas de protesta y resistencia de los trabajadores en el largo plazo, considerando la interacción entre coacción, mercado y acciones colectivas, las huelgas anteriores a 1878, las disputas laborales en el ámbito militar, y otras formas de conflicto.

Bamboo is a non-timber forest product and one of the most important grass plants of industrial and domestic use. It is widely distributed in tropical countries including India, China and Southeast Asian countries with wide genetic diversity. The diversity in the available genotypes becomes an important resource for the selection and improvement of the plants for ecological and commercial use. This study investigates eight commercially and ecologically important bamboo species of six genera (Bambusa, Dendrocalamus, Thyrsostachys, Vietnamosasa, Cephalostachyum and Indocalamus) from India, Thailand and Laos. These were evaluated for genetic differences by molecular makers, chemo-morphological variation and ability of silicon accumulation. The genetic cluster analyses of eight RAPD primers revealed genetic similarities in the ranges of 24-55%. The total silica content varied from 18.34 to 40.08 ppm in leaves of different bamboo species. Chemical analysis of the silicon content by ICP-OES and secondary metabolite profiling on TLC depicted the prominent distinction among the species. The PCA analysis of quantitative morphological data grouped the species in two major clusters and found to correlate with chemical pattern and genetic similarity to some extent. This is the first report that summarizes species-specific variability of leaf silica content, secondary metabolites, and quantitative morphological data towards delineation of genetic phylogeny of bamboo species.

Various natural and anthropogenic activities affect the land ecosystem which results in the loss of cultivated land, forest, or grazing land, leading to the overall loss of production. The eco-rejuvenation of such degraded lands in practice can largely be considered as ecosystem restoration or the reestablishment of the capability of the land to capture and retain its fundamental resources. Eco-rejuvenation is the biotechnological approach by which a degraded ecosystem can be rejuvenated to its top successional stage. An important goal of sustainable biodiversity development on degraded land in rural areas is to accelerate natural successional processes (above- and below-ground biomass) to increase biological productivity, soil fertility, and biotic control over biogeochemical fluxes within the recovering ecosystems. Eco-rejuvenation is a recent and very effective technology for the development of biodiversity on the degraded lands. An ecosystem development model is discussed where the role of all influencing factors are taken into consideration for the development of biodiversity on various types of degraded/wasteland. There are several environmental and socioeconomic benefits of eco-rejuvenation technology which include the ecological restoration of wasteland productivity, the high survival rate of selected plant species, an increase in biomass, mitigation of the climate change problem, and carbon dioxide sequestration.

In preparation for remote atmospheric detection, preliminary double resonance spectroscopic measurements were made of CH3Cl and CH3F using a LWIR pump to excite transient vibrational states and a THz source to probe the rotational levels.

Objectives To analyse prenatal parameters predicting biventricular (BV) outcome in pulmonary atresia with intact ventricular septum/critical pulmonary stenosis (PAIVS/CPS). Methods We evaluated 82 foetuses from 01/08 to 10/18 in 3 centres in intervals 1 ( 30 weeks). Results 61/82 (74.4%) were livebirths, 5 (8.2%) lost for follow-up, 3 (4.9%) had compassionate care leaving 53 (64.6% of the whole cohort and 86.9% of livebirths) with intention to treat. 9 died, 44/53 (83.0%) survived. 24/38 (63.2%) with information on postnatal outcome had BV outcome, 14 (36.8%) non-BV outcome (2 × 1.5 circulation). One with BV outcome had prenatal valvuloplasty. Best single parameter for BV outcome was tricuspid/mitral valve (TV/MV) ratio (AUC 0.93) in intervals 2 and 3 (AUC 0.92). Ventriculo-coronary-arterial communications (VCAC) were present in 11 (78.6%) in non-BV outcome group vs. 2 (8.3%) in BV outcome group (p 2.5 m/s was present in BV outcome group in75.0% (18/24) vs. 14.3% (2/14) in non-BV outcome group. Including the most predictive markers (VCAC presence, TI- Vmax 1 criterion was fulfilled in all cases. After recently published criteria for foetal intervention, only 4/9 (44.4%) and 5/14 (35.7%) in our interval 2 + 3 with predicted non-BV outcome would have been candidates for intervention. Two (1 × intrauterine intervention) in interval 2, two in interval 3 reached BV outcome and one 1.5 circulation without intervention. Conclusion TV/MV ratio as simple parameter has high predictive value. After our score, non-BV outcome was correctly predicted in all cases. Criteria for foetal intervention must further be evaluated.

Decomposing scenes into reflectance and lighting is an important task for applications such as relighting, image matching or content creation. Advanced light transport effects like occlusion and indirect lighting are often ignored, leading to subpar decompositions in which the albedo needs to compensate for insufficiencies in the estimated shading. We show how to account for these advanced lighting effects by utilizing precomputed radiance transfer to estimate reflectance and lighting. Given the geometry of an object and one or multiple images, our method reconstructs the object’s surface reflectance properties—such as its albedo and glossiness—as well as a colored lighting environment map. Evaluation on synthetic and real data shows that incorporation of indirect light leads to qualitatively and quantitatively improved results.

Data from each subject in a repeated-measures experiment form a time series, which may include trial-by-trial fluctuations arising from human factors such as practice or fatigue. Concerns about the statistical implications of such effects have increased the popularity of Generalized Additive Mixed Models (GAMMs), a powerful technique for modeling wiggly patterns. We question these statistical concerns and investigate the costs and benefits of using GAMMs relative to linear mixed-effects models (LMEMs). In two sets of Monte Carlo simulations, LMEMs that ignored time-varying effects were no more prone to false positives than GAMMs. Although GAMMs generally boosted power for within-subject effects, they reduced power for between-subject effects, sometimes to a severe degree. Our results signal the importance of proper subject-level randomization as the main defense against statistical artifacts due to by-trial fluctuations.

The present study entails the phytoremediation potential of different bamboo species on 5-year-old FA-dumped site near Koradi thermal power plant of Nagpur, Maharashtra, India. The selected FA-dumped site was treated with farmyard manure, press mud, and bio fertilizer followed by plantation of six promising species of bamboo namely Bambusa balcooa Roxb., Dendrocalamus stocksii (Munro.) M. Kumar, Remesh and Unnikrishnan, Bambusa bambos (L.) Voss, Bambusa wamin E.G. Camus, Bambusa vulgaris var. striata (Lodd. ex Lindl.) Gamble, and Bambusa vulgaris var. vittata Riviere and Riviere. The experimental results indicated that the organic input in the FA-dumped site nourished the soil by improving its physico-chemical, and biological characteristics. The results revealed the contamination of the site with different trace elements in varied quantity including Cr (89.29 mg kg

BACKGROUND: The therapeutic success in patients with congenital heart disease (CHD) leads to a growing number of adults with CHD (adult CHD [ACHD]) who develop end-stage heart failure. We aimed to determine patient characteristics and outcomes of ACHD listed for heart transplantation. METHODS: Using data from all the patients with ACHD in 20 transplant centers in the Eurotransplant region from 1999 to 2015, we analyzed patient characteristics, waiting list, and post-transplantation outcomes. RESULTS: A total of 204 patients with ACHD were listed during the study period. The median age was 38 years, and 62.3% of the patients were listed in high urgency (HU), and 37.7% of the patients were in transplantable (T)-listing status. A total of 23.5% of the patients died or were delisted owing to clinical worsening, and 75% of the patients underwent transplantation. Median waiting time for patients with HU-listing status was 4.18 months and with T-listing status 9.07 months. There was no difference in crude mortality or delisting between patients who were HU status listed and T status listed (p = 0.65). In multivariable regression analysis, markers for respiratory failure (mechanical ventilation, hazard ratio [HR]: 1.41, 95% CI: 1.11-1.81, p = 0.006) and arrhythmias (anti-arrhythmic medication, HR: 1.42, 95% CI: 1.01-2.01, p = 0.044) were associated with a higher risk of death or delisting. In the overall cohort, post-transplantation mortality was 26.8% after 1 year and 33.4% after 5 years. CONCLUSIONS: Listed patients are at high risk of death without differences in the urgency of listing. Respiratory failure requiring invasive ventilation and possibly arrhythmias requiring anti-arrhythmic medication indicate worse outcomes on waiting list. (C) 2020 International Society for Heart and Lung Transplantation. All rights reserved.

The transition from student nurse to nurse graduate can be overwhelming. Although nurse residency programs ease this transition, standardized patient (SP) simulations supplementing these programs are underutilized. An SP simulation series was created and imbedded into a behavioral health nurse residency program. Nurse residents were satisfied with the learning experience and gained self-confidence, which was sustained over 30 days. Staff development educators should consider supplementing a nurse residency program with an SP simulation to hone behavioral health clinical skill application.

After a century of research, the human centrosome continues to fascinate. Based on immunofluorescence and confocal microscopy, an extensive inventory of the protein components of the human centrosome, and the centriolar satellites, with the important contribution of over 300 novel proteins localizing to these compartments is presented. A network of candidate centrosome proteins involved in ubiquitination, including six interaction partners of the Kelch-like protein 21, and an additional network of protein phosphatases, together supporting the suggested role of the centrosome as an interactive hub for cell signaling, is identified. Analysis of multi-localization across cellular organelles analyzed within the Human Protein Atlas (HPA) project shows how multi-localizing proteins are particularly overrepresented in centriolar satellites, supporting the dynamic nature and wide range of functions for this compartment. In summary, the spatial dissection of the human centrosome and centriolar satellites described here provides a comprehensive knowledgebase for further exploration of their proteomes.

The nucleolus is essential for ribosome biogenesis and is involved in many other cellular functions. We performed a systematic spatiotemporal dissection of the human nucleolar proteome using confocal microscopy. In total, 1,318 nucleolar proteins were identified; 287 were localized to fibrillar components, and 157 were enriched along the nucleoplasmic border, indicating a potential fourth nucleolar subcompartment: the nucleoli rim. We found 65 nucleolar proteins (36 uncharacterized) to relocate to the chromosomal periphery during mitosis. Interestingly, we observed temporal partitioning into two recruitment phenotypes: early (prometaphase) and late (after metaphase), suggesting phase‐specific functions. We further show that the expression of MKI67 is critical for this temporal partitioning. We provide the first proteome‐wide analysis of intrinsic protein disorder for the human nucleolus and show that nucleolar proteins in general, and mitotic chromosome proteins in particular, have significantly higher intrinsic disorder level compared to cytosolic proteins. In summary, this study provides a comprehensive and essential resource of spatiotemporal expression data for the nucleolar proteome as part of the Human Protein Atlas., Spatiotemporal characterization of the human nucleolar proteome reveals spatial partitioning into fibrillar components and nucleoli rim. A subset of proteins with high intrinsic disorder show temporal relocation to the chromosomal periphery during mitosis.

The correspondence between mathematical structures and experimental systems is the basis of the generalizability of results found with specific systems and is the basis of the predictive power of theoretical physics. While physicists have confidence in this correspondence, it is less recognized in cellular biophysics. On the one hand, the complex organization of cellular dynamics involving a plethora of interacting molecules and the basic observation of cell variability seem to question its possibility. The practical difficulties of deriving the equations describing cellular behaviour from first principles support these doubts. On the other hand, ignoring such a correspondence would severely limit the possibility of predictive quantitative theory in biophysics. Additionally, the existence of functional modules (like pathways) across cell types suggests also the existence of mathematical structures with comparable universality. Only a few cellular systems have been sufficiently investigated in a variety of cell types to follow up these basic questions. IP

Cancer cells exhibit increased motility and proliferation, which are instrumental in the formation of tumours and metastases. These pathological changes can be traced back to malfunctions of cellular signalling pathways, and calcium signalling plays a prominent role in these. We formulate a new model for cancer cell movement which for the first time explicitly accounts for the dependence of cell proliferation and cell-cell adhesion on calcium. At the heart of our work is a non-linear, integro-differential (non-local) equation for cancer cell movement, accounting for cell diffusion, advection and proliferation. We also employ an established model of cellular calcium signalling with a rich dynamical repertoire that includes experimentally observed periodic wave trains and solitary pulses. The cancer cell density exhibits travelling fronts and complex spatial patterns arising from an adhesion-driven instability (ADI). We show how the different calcium signals and variations in the strengths of cell-cell attraction and repulsion shape the emergent cellular aggregation patterns, which are a key component of the metastatic process. Performing a linear stability analysis, we identify parameter regions corresponding to ADI. These regions are confirmed by numerical simulations, which also reveal different types of aggregation patterns and these patterns are significantly affected by \ca. Our study demonstrates that the maximal cell density decreases with calcium concentration, while the frequencies of the calcium oscillations and the cell density oscillations are approximately equal in many cases. Furthermore, as the calcium levels increase the speed of the travelling fronts increases, which is related to a higher cancer invasion potential. These novel insights provide a step forward in the design of new cancer treatments that may rely on controlling the dynamics of cellular calcium., Comment: 27 pages; 8 figures