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Introduction: Venous thromboembolic disease is one of the leading causes of morbidity and mortality in the developed world. Identification of hereditary factors of thrombophilia is contributing to a better understanding of the etiology and prevention of the disease. The aim of this study was to assess the prevalence of factor II G20210A, factor V Leiden, MTHFR C677T and PAI-1 5G/4G polymorphisms in healthy Croatian subjects and in patients with thromboembolism. Materials and methods: This prospective study included 106 healthy subjects and 100 thromboembolic patients who were consecutively admitted to the Intensive Care Unit, University Hospital "Sestre milosrdnice". Results: Heterozygotes for Factor V Leiden polymorphism were more frequent in the group of patients with the thromboembolic disease (16%) than in the control healthy subjects (2.9%), with OR 6.413 (1.807 - 22.758 ; P = 0.004). Allele and genotype frequencies of other studied polymorphisms did not differ between cases and controls. Conclusion: This study confirmed the association of factor V Leiden polymorphism with the development of the thromboembolic disease in Croatian population.

Preeklampsija je vodeći je uzrok maternalnog i neonatalnog morbiditeta i mortaliteta i teško je predvidjeti pomoću trenutno dostupnih kliničkih testova. Cilj rada je bio ispitati prediktivni potencijal standardnih biokemijskih, hematoloških i antropometrijskih parametara te novih angiogenih čimbenika za nastanak preeklampsije putem dvije studije: prve retrospektivne, uz pomoć standardnih laboratorijskih parametara u trećem tromjesečju trudnoće i druge, prospektivne, uz pomoć novih angiogenih čimbenika (PlGF, sFlt-1) nakon 20-og tjedna trudnoće. U prvoj studiji (113 trudnica s preeklampsijom, te kontrolna skupina od 95 trudnica s urednom trudnoćom) uz pomoć multivarijantne logističke regresije u modelu koji uključuje urate i ureu ispravno je klasificirano 79,6% sudionica. Dodavanjem trombocita, hematokrita, AST i leukocita ovom modelu ispravno je klasificirano 83,8% sudionica. U drugoj studiji (34 trudnice s preeklampsijom, te 35 trudnica s urednom trudnoćom), na identičan način ispitivani su i obrađeni isti parametri, uz dodatak novih angiogenih čimbenika čime se postotak točno klasificiranih sudionica u osnovnom modelu (urati, PlGF, sFlt-1) povećao na 85,07%, u modelu s dodatkom indeksa tjelesne mase prije poroda na 88,06%, te u modelu s dodatkom indeksa površine tijela na 91,4%. Dobiveni rezultati optimalnog laboratorijskog panela sastavljenog od izabranih standardnih laboratorijskih parametara potvrđuju visok prediktivni potencijal u predviđanju preeklampsije u trećem tromjesečju. Novi angiogeni čimbenici pokazali su se superiornijim od standardnih parametara te pomiču sposobnost predikcije nastanka preeklampsije u nižu gestacijsku dob. Preeclampsia is a leading cause of maternal and neonatal morbidity and mortality and is difficult to predict with available clinical tests. The aim of this research was to investigate the predictive potential of standard biochemical, hematological and anthropometric parameters together with new angiogenic markers in prediction of preeclampsia through two studies: first, retrospective by employing standard laboratory parameters at third trimester of pregnancy and second prospective study with new angiogenic markers (PlGF, sFlt-1) after 20 weeks of pregnancy. In the first study (113 patients with preeclampsia and a control group of 95 uncomplicated pregnancies) by the use of multivariate logistic regression model with uric acid and BUN included, 79.6% patients were classified correctly. By adding thrombocytes, hematocrit, AST and leukocytes to this model, 83.8% of participants were correctly classified. In the second study (34 patients with preeclampsia and 35 patients with uncomplicated pregnancy) the same parameters were analysed and evaluated with the addition of new angiogenic markers. It elevated the percentage of pregnancies correctly classified in basic model (uric acid, PlGF, sFlt-1) to 85.07%, in a model with addition of body mass index before childbirth up to 88.06%, and in a model with addition of body surface area to 91.4%, respectively. Results of an optimal laboratory panel composed of selected standard laboratory parameters confirm high predictive potential in preeclampsia prediction in third trimester. New angiogenic markers appeared to be superior to standard parameters and shift prediction potential of preeclampsia to lower gestational age.