Search

Additional file 1: Figures S1. In vitro PARylation of TETs. Figure S2. Co-immunoprecipitation of TET1 and PARP-1 from NIH3T3 cell lysates. Figure S3. MTT viability assay. Supplementary Figure S4. Level of 5hmC in control and ascorbic acid treated NIH3T3cells.

Additional file 3: Materials and methods: ELISA-based plate assay for DNA hydroxymethylation analysis, determination of 5hmC level by immunocytochemistry and co-immunoprecipitation.

Additional file 2: Table S1. Statistical significance of 5hmC level differences between groups evaluated by nested ANOVA followed by Tukey post hoc test.

A measurement is presented of electroweak (EW) production of a W boson in association with two jets in proton–proton collisions at s=13Te. The data sample was recorded by the CMS Collaboration at the LHC and corresponds to an integrated luminosity of 35.9fb-1. The measurement is performed for the ℓνjj final state (with ℓν indicating a lepton–neutrino pair, and j representing the quarks produced in the hard interaction) in a kinematic region defined by invariant mass mjj>120Ge and transverse momenta pTj>25Ge. The cross section of the process is measured in the electron and muon channels yielding σEW(Wjj)=6.23±0.12(stat)±0.61(syst)pb per channel, in agreement with leading-order standard model predictions. The additional hadronic activity of events in a signal-enriched region is studied, and the measurements are compared with predictions. The final state is also used to perform a search for anomalous trilinear gauge couplings. Limits on anomalous trilinear gauge couplings associated with dimension-six operators are given in the framework of an effective field theory. The corresponding 95% confidence level intervals are -2.3

A search for WW production from double-parton scattering processes using same-charge electron-muon and dimuon events is reported, based on proton-proton collision data collected at a center-of-mass energy of 13TeV. The analyzed data set corresponds to an integrated luminosity of 77.4fb-1, collected using the CMS detector at the LHC in 2016 and 2017. Multivariate classifiers are used to discriminate between the signal and the dominant background processes. A maximum likelihood fit is performed to extract the signal cross section. This leads to the first evidence for WW production via double-parton scattering, with a significance of 3.9 standard deviations. The measured inclusive cross section is 1.41±0.28(stat)±0.28(syst)pb.

Central exclusive and semiexclusive production of pairs is measured with the CMS detector in proton-proton collisions at the LHC at center-of-mass energies of 5.02 and 13TeV. The theoretical description of these nonperturbative processes, which have not yet been measured in detail at the LHC, poses a significant challenge to models. The two pions are measured and identified in the CMS silicon tracker based on specific energy loss, whereas the absence of other particles is ensured by calorimeter information. The total and differential cross sections of exclusive and semiexclusive central production are measured as functions of invariant mass, transverse momentum, and rapidity of the system in the fiducial region defined as transverse momentum and pseudorapidity . The production cross sections for the four resonant channels , , , and are extracted using a simple model. These results represent the first measurement of this process at the LHC collision energies of 5.02 and 13TeV. CMS Collaboration (ukupan broj autora: 2326)

A search is performed for neutral non-standard-model Higgs bosons decaying to two muons in the context of the minimal supersymmetric standard model (MSSM). Proton-proton collision data recorded by the CMS experiment at the CERN Large Hadron Collider at a center-of-mass energy of 13TeVwere used, corresponding to an integrated luminosity of 35.9fb-1. The search is sensitive to neutral Higgs bosons produced via the gluon fusion process or in association with a bbquark pair. No significant deviations from the standard model expectation are observed. Upper limits at 95% confidence level are set in the context of the mmod+hand phenomenological MSSM scenarios on the parameter tanβas a function of the mass of the pseudoscalar Aboson, in the range from 130 to 600GeV. The results are also used to set a model-independent limit on the product of the branching fraction for the decay into a muon pair and the cross section for the production of a scalar neutral boson, either via gluon fusion, or in association with bquarks, in the mass range from 130 to 1000GeV.

A measurement for inclusive 2- and 3-jet events of the azimuthal correlation between the two jets with the largest transverse momenta, Δϕ12, is presented. The measurement considers events where the two leading jets are nearly collinear (“back-to-back”) in the transverse plane and is performed for several ranges of the leading jet transverse momentum. Proton-proton collision data collected with the CMS experiment at a center-of-mass energy of 13Te and corresponding to an integrated luminosity of 35.9fb-1 are used. Predictions based on calculations using matrix elements at leading-order and next-to-leading-order accuracy in perturbative quantum chromodynamics supplemented with leading-log parton showers and hadronization are generally in agreement with the measurements. Discrepancies between the measurement and theoretical predictions are as large as 15%, mainly in the region 177 ∘< Δϕ12< 180 ∘. The 2- and 3-jet measurements are not simultaneously described by any of models.

Kičica se u Srbiji tradicionalno koristi za lečenje različitih oboljenja, uključujući i dijabetes. Cilj ovog rada bio je rasvetljavanje mehanizama antioksidativnog i protektivnog dejstva ekstrakta kičice (EK) koji doprinose očuvanju strukture i funkcije β-ćelija pankreasa i ublažavanju komplikacija u dijabetesu. Efekti EK analizirani su na eksperimentalnom modelu dijabetesa pacova izazvanom streptozotocinom (STZ) kao i na Rin-5F β-ćelijama izloženim oksidativnom stresu primenom vodonik peroksida, natrijum nitroprusida ili STZ-a. EK je ispoljio antidijabetogeno dejstvo uočeno kao sniženje nivoa glukoze/glikohemoglobina, poboljšanje lipidnog statusa i povećanje nivoa insulina u cirkulaciji dijabetičnih pacova što se može pripisati detektovanom uticaju EK na oc uvanje strukture Langerhansovih ostrvaca i povec anju mase β-c elija koje produkuju insulin. ntioksidativni efekat EK na β-c elije ogledao se u redukovanju oksidativnih os tec enja, modulisanju aktivnosti i ekspresije enzima antioksidativne zaštite (C T, SOD, GPx i GR) i uravnotežavanju aktivnosti redoks-senzitivnih faktora (NFκB, Nrf-2, Sp1, FOXO3 ) uključenih u regulaciju transkripcije gena za antioksidativne enzime. Dodatni doprinos EK boljem preživljavanju β-ćelija i sekreciji/ekspresiji insulina ostvaren je finim promenama u aktivnosti Akt, ERK i p38 kinaza kao i PDX-1 i MafA proteina. Pored toga, antioksidativni efekat EK kod dijabetičnih životinja ogledao se u zaštiti eritrocita, jetre i bubrega od gliko-oksidativnih oštećenja što je doprinelo poboljšanju njihovih funkcionalnih parametara. Opisani efekti EK ukazuju na značaj daljeg razjašnjavanja mehanizama antioksidativnog i protektivnog dejstva EK i njegovih komponenti u cilju potencijalnog terapijskog delovanja u dijabetesu. Centaury is traditionally used in Serbia for treating different diseases, including diabetes. This study aimed to elucidate the mechanisms of the antioxidant and protective effects of the centaury extract (CE) that preserve the structure and function of pancreatic β-cells and alleviate diabetic complications. The effects of the CE were analyzed on the experimental model of streptozotocin (STZ)-induced diabetic rats and in Rin-5F β-cells exposed to oxidative stress after exposure to hydrogen peroxide, sodium nitroprusside or STZ. The CE exhibited an anti-diabetic effect, observed as a decrease in hyperglycemia and glycohemoglobin concentration, an improved lipid status, and as increased insulin level in the circulation of diabetic rats. These effects can be attributed to the detected impact of the CE on the preservation of the structure of the islets of Langerhans, and on the increase in insulin producing β-cell mass. The antioxidant effect of the CE on β-cells was manifested as a reduction in oxidative damage, changed activities and expression of antioxidant enzymes, CAT, SOD, GPx and GR, and as a readjustment of the activities of redox-sensitive factors, NFκB, Nrf-2, Sp1, FOXO3A, involved in the regulation of gene transcription of antioxidant enzymes. Additional contributions of the CE to improved β-cell survival and insulin secretion/expression were achieved by the fine-tuning of the activities of Akt, ERK and p38 kinases and transcription regulators, PDX-1 and MafA. The antioxidant effect of the CE in diabetic animals was also reflected on increased protection of erythrocytes, liver and kidneys from glyco-oxidative damage, which contributed to the improvement of their functional parameters in diabetic rats. The described effects of the CE point to the importance of further clarification of the mechanisms of the antioxidant and general protective effects of the CE and its components in view of its potential use for therapeutic intervention in diabetes.

Measurements of differential cross sections for inclusive very forward jet production in proton-lead collisions as a function of jet energy are presented. The data were collected with the CMS experiment at the LHC in the laboratory pseudorapidity range −6.6 < η < −5.2. Asymmetric beam energies of 4 TeV for protons and 1.58 TeV per nucleon for Pb nuclei were used, corresponding to a center-of-mass energy per nucleon pair of sNN = 5.02 TeV. Collisions with either the proton (p+Pb) or the ion (Pb+p) traveling towards the negative η hemisphere are studied. The jet cross sections are unfolded to stable-particle level cross sections with p T ≳ 3 GeV, and compared to predictions from various Monte Carlo event generators. In addition, the cross section ratio of p+Pb and Pb+p data is presented. The results are discussed in terms of the saturation of gluon densities at low fractional parton momenta. None of the models under consideration describes all the data over the full jet-energy range and for all beam configurations. Discrepancies between the differential cross sections in data and model predictions of more than two orders of magnitude are observed.[Figure not available: see fulltext.]. © 2019, The Author(s).

The pseudorapidity distributions of charged hadrons in proton-lead collisions at nucleon-nucleon center-of-mass energies root s(NN) = 5.02 and 8.16 TeV are presented. The measurements are based on data samples collected by the CMS experiment at the LHC. The number of primary charged hadrons produced in non-single-diffractive proton-lead collisions is determined in the pseudorapidity range vertical bar eta(lab)vertical bar LT 2.4. The charged-hadron multiplicity distributions are compared to the predictions from theoretical calculations and Monte Carlo event generators. In the center-of-mass pseudorapidity range vertical bar eta(cm)vertical bar LT 0.5, the average charged-hadron multiplicity densities LT dN(ch)/d eta(cm) GT vertical bar(vertical bar eta cm vertical bar) LT 0.5 are 17.1 +/- 0.01 (stat) +/- 0.59 (syst) and 20.10 +/- 0.01 (stat) +/- 0.5(syst) at root s(NN) = 5.02 and 8.16 TeV, respectively. The particle densities per participant nucleon are compared to similar measurements in proton-proton, proton-nucleus, and nucleus-nucleus collisions.

The cross sections for the production of t (t) over bar b (b) over bar and t (t) over bar jj events and their ratio sigma(t (t) over bar b (b) over bar)/sigma(t (t) over bar jj) are measured using data corresponding to an integrated luminosity of 2.3 fb(-1) collected in pp collisions at root s = 13 TeV with the CMS detector at the LHC. Events with two leptons (e or mu) and at least four reconstructed jets, including at least two identified as b quark jets, in the final state are selected. In the full phase space, the measured ratio is 0.022 +/- 0.003 (stat) +/- 0.006 (syst), the cross section sigma(t (t) over bar b (b) over bar) bis 4.0 +/- 0.6 (stat)+/- 1.3 (syst) pb and sigma(t (t) over bar jj) is 184 +/- 6 (stat)+/- 33 (syst) pb. The measurements are compared with the standard model expectations obtained from a POWHEG simulation at next-to-leading-order interfaced with PYTHIA. (c) 2017 The Author. Published by Elsevier B.V.

Results are reported from a search for new physics in 13 TeV proton-proton collisions in the final state with large missing transverse momentum and two Higgs bosons decaying via H - GT b(b)over bar. The search uses a data sample accumulated by the CMS experiment at the LHC in 2016, corresponding to an integrated luminosity of 35.9 fb(-1). The search is motivated by models based on gauge-mediated supersymmetry breaking, which predict the electroweak production of a pair of Higgsinos, each of which can decay via a cascade process to a Higgs boson and an undetected lightest supersymmetric particle. The observed event yields in the signal regions are consistent with the standard model background expectation obtained from control regions in data. Higgsinos in the mass range 230-770 GeV are excluded at 95% confidence level in the context of a simplified model for the production and decay of approximately degenerate Higgsinos.

A search is presented for narrow heavy resonances decaying to a top quark and a bottom quark using data collected by the CMS experiment at root s= 13 TeV in 2016. The data set analyzed corresponds to an integrated luminosity of 35.9fb(-1). Final states that include a single lepton (e, mu), multiple jets, and missing transverse momentum are analyzed. No evidence is found for the production of a W boson, and the production of right-handed W bosons is excluded at 95% confidence level for masses up to 3.6 TeV depending on the scenario considered. Exclusion limits for W bosons are also presented as a function of their coupling strength to left- and right-handed fermions. These limits on a W boson decaying via a top and a bottom quark are the most stringent published to date. (c) 2017 The Author. Published by Elsevier B.V.

A search for supersymmetry is presented based on proton-proton collision events containing identified hadronically decaying top quarks, no leptons, and an imbalance p(T)(miss) in transverse momentum. The data were collected with the CMS detector at the CERN LHC at a center-of-mass energy of 13 TeV, and correspond to an integrated luminosity of 35.9 fb(-1). Search regions are defined in terms of the multiplicity of bottom quark jet and top quark candidates, the p(T)(miss) , the scalar sum of jet transverse momenta, and themT2 mass variable. No statistically significant excess of events is observed relative to the expectation from the standard model. Lower limits on the masses of supersymmetric particles are determined at 95% confidence level in the context of simplified models with top quark production. For a model with direct top squark pair production followed by the decay of each top squark to a top quark and a neutralino, top squark masses up to 1020 GeVand neutralino masses up to 430 GeVare excluded. For amodel with pair production of gluinos followed by the decay of each gluino to a top quark-antiquark pair and a neutralino, gluino masses up to 2040 GeVand neutralino masses up to 1150 GeVare excluded. These limits extend previous results.

The Fourier coefficients v(2) and v(3) characterizing the anisotropy of the azimuthal distribution of charged particles produced in PbPb collisions at root S-NN = 5.02 TeV are measured with data collected by the CMS experiment. The measurements cover a broad transverse momentum range, 1 LT p(T) LT 100 GeV/c. The analysis focuses on the p(T) GT 10 GeV/c range, where anisotropic azimuthal distributions should reflect the path-length dependence of parton energy loss in the created medium. Results are presented in several bins of PbPb collision centrality, spanning the 60% most central events. The v(2) coefficient is measured with the scalar product and the multiparticle cumulant methods, which have different sensitivities to initial-state fluctuations. The values from both methods remain positive up to p(T) similar to 60-80 GeV/c, in all examined centrality classes. The v(3) coefficient, only measured with the scalar product method, tends to zero for p(T) greater than or similar to 20 GeV/c. Comparisons between theoretical calculations and data provide new constraints on the path-length dependence of parton energy loss in heavy ion collisions and highlight the importance of the initial-state fluctuations. (C) 2017 The Author. Published by Elsevier B.V.

A search for narrow vector resonances decaying into quark-antiquark pairs is presented. The analysis is based on data collected in proton-proton collisions at root s = 13 TeV with the CMS detector at the LHC, corresponding to an integrated luminosity of 35.9 fb(-1). The hypothetical resonance is produced with sufficiently high transverse momentum that its decay products are merged into a single jet with two-prong substructure. A signal would be identified as a peak over a smoothly falling background in the distribution of the invariant mass of the jet, using novel jet substructure techniques. No evidence for such a resonance is observed within the mass range of 50-300 GeV. Upper limits at 95% confidence level are set on the production cross section, and presented in a mass-coupling parameter space. The limits further constrain simplified models of dark matter production involving a mediator interacting between quarks and dark matter particles through a vector or axial-vector current. In the framework of these models, the results are the most sensitive to date, extending for the first time the search region to masses below 100 GeV.

In the original paper, figure 10 was incorrect. The correct figure is shown below. Additionally, the unparticle entries in table 3, as well as figure 4 and 5 were labelled with incorrect values of ΛU. The entries correctly correspond to ΛU = 1 TeV. Link to Erratum 1: [https://vinar.vin.bg.ac.rs/handle/123456789/1760] Link to the original article: [https://vinar.vin.bg.ac.rs/handle/123456789/10985]

A search for heavy narrow resonances decaying into four-lepton final states has been performed using proton-proton collision data at root s= 8 TeVcollected by the CMS experiment, corresponding to an integrated luminosity of 19.7fb(-1). No excess of events over the standard model background expectation is observed. Upper limits for a benchmark model on the product of cross section and branching fraction for the production of these heavy narrow resonances are presented. The limit excludes leptophobic Z bosons with masses below 2.5 TeV within the benchmark model. This is the first result to constrain a leptophobic Z resonance in the four-lepton channel. (C) 2017 The Author(s). Published by Elsevier B.V.

A measurement of the top quark mass (M-t) in the dileptonic t (t) over bar decay channel is performed using data from proton-proton collisions at a center-of-mass energy of 8 TeV. The data was recorded by the CMS experiment at the LHC and corresponds to an integrated luminosity of 19.7 +/- 0.5 fb(-1). Events are selected with two oppositely charged leptons (l = e, mu) and two jets identified as originating from b quarks. The analysis is based on three kinematic observables whose distributions are sensitive to the value of Mt. An invariant mass observable, M-bl, and a stransverse mass observable, M-T2, are employed in a simultaneous fit to determine the value of M-t and an overall jet energy scale factor (JSF). A complementary approach is used to construct an invariant mass observable, M-blv, that is combined with M-T2 to measure M-t. The shapes of the observables, along with their evolutions in M-t and JSF, are modeled by a nonparametric Gaussian process regression technique. The sensitivity of the observables to the value of M-t is investigated using a Fisher information density method. The top quark mass is measured to be 172.22 +/- 0.18(stat)(-0.93)(+0.89) (syst) GeV.

In the original paper, figure 10 was incorrect. The correct figure is shown above. Link to Erratum 2: [https://vinar.vin.bg.ac.rs/handle/123456789/1921] Link to the original article: [https://vinar.vin.bg.ac.rs/handle/123456789/10985]

Pair production of gamma(1S) mesons is observed at the LHC in proton-proton collisions at root s = 8 TeV by the CMS experiment in a data sample corresponding to an integrated luminosity of 20.7 fb(-1). Both gamma(1S) candidates are fully reconstructed via their decays to mu(+) mu(-). The fiducial acceptance region is defined by an absolute gamma(1S) rapidity smaller than 2.0. The fiducial cross section for the production of gamma(1S) pairs, assuming that both mesons decay isotropically, is measured to be 68.8 +/- 12.7 (stat) +/- 7.4 (syst) +/- 2.8 (B) pb, where the third uncertainty comes from the uncertainty in the branching fraction of gamma(1S) decays to mu(+) mu(-). Assuming instead that the gamma(1S) mesons are produced with different polarizations leads to variations in the measured cross section in the range from 38% to +36%.

A measurement of the top quark pair production () cross section in proton-proton collisions at the centre-of-mass energy of 8 is presented using data collected with the CMS detector at the LHC, corresponding to an integrated luminosity of 19.6. This analysis is performed in the decay channels with one isolated, high transverse momentum electron or muon and at least four jets, at least one of which is required to be identified as originating from hadronization of a b quark. The calibration of the jet energy scale and the efficiency of b jet identification are determined from data. The measured cross section is . This measurement is compared with an analysis of 7 data, corresponding to an integrated luminosity of 5.0, to determine the ratio of 8 to 7 cross sections, which is found to be . The measurements are in agreement with QCD predictions up to next-to-next-to-leading order.

A search for dark matter particles is performed using events with large missing transverse momentum, at least one energetic jet, and no leptons, in proton-proton collisions at root S = 13TeV collected with the CMS detector at the LHC. The data sample corresponds to an integrated luminosity of 12.9 fb(-1). The search includes events with jets from the hadronic decays of a W or Z boson. The data are found to be in agreement with the predicted background contributions from standard model processes. The results are presented in terms of simpli fi ed models in which dark matter particles are produced through interactions involving a vector, axial-vector, scalar, or pseudoscalar mediator. Vector and axial-vector mediator particles with masses up to 1.95TeV, and scalar and pseudoscalar mediator particles with masses up to 100 and 430 GeV respectively, are excluded at 95% con fi dence level. The results are also interpreted in terms of the invisible decays of the Higgs boson, yielding an observed (expected) 95% con fi dence level upper limit of 0.44 (0.56) on the corresponding branching fraction. The results of this search provide the strongest constraints on the dark matter pair production cross section through vector and axial-vector mediators at a particle collider. When compared to the direct detection experiments, the limits obtained from this search provide stronger constraints for dark matter masses less than 5, 9, and 550 GeV, assuming vector, scalar, and axial-vector mediators, respectively. The search yields stronger constraints for dark matter masses less than 200 GeV, assuming a pseudoscalar mediator, when compared to the indirect detection results from Fermi-LAT.

Introduction: Trachoma is the most common cause of infectious blindness worldwide, initiated by repeated infection of the conjunctiva with Chlamydia trachomatis (Ct). The resulting chronic inflammation and formation of fibrotic tissue eventually lead to corneal damage. Based on the facts that epithelial to mesenchymal transition (EMT) plays an important role in the development of fibrosis and that EMT is epigenetically regulated process, the aims of this study were to reveal the capacity of Ct to induce EMT in vitro and to unveil potential underlying epigenetic mechanisms. Methods: Human conjunctival epithelial (HCjE) cells were infected with 107 IFU of Ct for 72 h. EMT-inducing signaling pathways, as well as mRNA and protein expression of EMT markers (E-cadherin, fibronectin and α-SMA) were evaluated by RT-qPCR, Immunoblotting and Immunocytochemistry. DNA methylation patterns of selected regions of E-cadherin, fibronectin and α-SMA genes were examined by Methylation-Specific PCR, High Resolution Melting analysis and Bisulfite Sequencing. Results: Infection with Ct was accompanied with the activation of EMT-inducing signaling pathways, downregulation of epithelial marker E-cadherin and upregulation of mesenchymal markers fibronectin and α-SMA. While DNA methylation status of E-cadherin gene promoter correlated with its expression, methylation status of fibronectin and α-SMA genes couldn’t be related to their expression levels. Conclusion: Ct infection of HCjE cells triggers EMT that goes along with changes in the methylation profile of the E-cadherin promoter. Sequence of events described herein could contribute to scarring process in trachoma and open up possibilities for development of new therapeutic strategies in trachoma treatment. Brajušković G, Đorđević A, editors. CoMBoS. 1st Congress of Molecular Biologists of Serbia; 2017 Sep 20-22; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Biology; 2017. p. 70.

Jet charge is an estimator of the electric charge of a quark, antiquark, or gluon initiating a jet. It is based on the momentum-weighted sum of the electric charges of the jet constituents. Measurements of three charge observables of the leading jet in transverse momentum pT are performed with dijet events. The analysis is carried out with data collected by the CMS experiment at the CERN LHC in proton-proton collisions at root s = 8 TeV corresponding to an integrated luminosity of 19.7 fb(-1). The results are presented as a function of the pT of the leading jet and compared to predictions from leading and next-to-leading-order event generators combined with parton showers. Measured jet charge distributions, unfolded for detector effects, are reported, which expand on previous measurements of the jet charge average and standard deviation in pp collisions.

Searches are presented for direct production of top or bottom squark pairs in proton-proton collisions at the CERN LHC. Two searches, based on complementary techniques, are performed in all-jet final states that are characterized by a significant imbalance in transverse momentum. An additional search requires the presence of a charged lepton isolated from other activity in the event. The data were collected in 2015 at a centre-of-mass energy of 13 TeV with the CMS detector and correspond to an integrated luminosity of 2.3 fb(-1). No statistically significant excess of events is found beyond the expected contribution from standard model processes. Exclusion limits are set in the context of simplified models of top or bottom squark pair production. Models with top and bottom squark masses up to 830 and 890 GeV, respectively, are probed for light neutralinos. For models with top squark masses of 675 GeV, neutralino masses up to 260 GeV are excluded at 95% confidence level.

A statistical combination of searches is presented for massive resonances decaying to WW, WZ, ZZ, WH, and ZH boson pairs in proton-proton collision data collected by the CMS experiment at the LHC. The data were taken at centre-of-mass energies of 8 and 13TeV, corresponding to respective integrated luminosities of 19.7 and up to 2.7 fb(-1). The results are interpreted in the context of heavy vector triplet and singlet models that mimic properties of composite-Higgs models predicting W and Z bosons decaying to WZ, WW, WH, and ZH bosons. A model with a bulk graviton that decays into WW and ZZ is also considered. This is the first combined search for WW, WZ, WH, and ZH resonances and yields lower limits on masses at 95% confidence level for W and Z singlets at 2.3 TeV, and for a triplet at 2.4TeV. The limits on the production cross section of a narrow bulk graviton resonance with the curvature scale of the warped extra dimension (K)over-tilde = 0.5, in the mass range of 0.6 to 4.0TeV, are the most stringent published to date. (c) 2017 The Author(s). Published by Elsevier B.V.

A measurement of the differential cross sections for a W boson produced in association with jets in the muon decay channel is presented. The measurement is based on 13 TeV proton-proton collision data corresponding to an integrated luminosity of 2.2 fb(-1), recorded by the CMS detector at the LHC. The cross sections are reported as functions of jet multiplicity, jet transverse momentum pT, jet rapidity, the scalar pT sum of the jets, and angular correlations between the muon and each jet for different jet multiplicities. The measured cross sections are in agreement with predictions that include multileg leading-order (LO) and next-to-LO matrix element calculations interfaced with parton showers, as well as a next-to-next-to-LO calculation for the W boson and one jet production.

A search is performed for anomalous interactions of the recently discovered Higgs boson using matrix element techniques with the information from its decay to four leptons and from associated Higgs boson production with two quark jets in either vector boson fusion or associated production with a vector boson. The data were recorded by the CMS experiment at the LHC at a center-of-mass energy of 13 TeV and correspond to an integrated luminosity of 38.6 fb(-1). They are combined with the data collected at center-of-mass energies of 7 and 8 TeV, corresponding to integrated luminosities of 5.1 and 19.7 fb(-1), respectively. All observations are consistent with the expectations for the standard model Higgs boson. (C) 2017 The Author. Published by Elsevier B.V.

A search for the production of heavy resonances decaying into top quark-antiquark pairs is presented. The analysis is performed in the lepton+jets and fully hadronic channels using data collected in proton-proton collisions at root s = 13 TeV using the CMS detector at the LHC, corresponding to an integrated luminosity of 2.6 fb(-1). The selection is optimized for massive resonances, where the top quarks have large Lorentz boosts. No evidence for resonant t (t) over bar production is found in the data, and upper limits on the production cross section of heavy resonances are set. The exclusion limits for resonances with masses above 2 TeV are significantly improved compared to those of previous analyses at root s = 8 TeV.

Trachoma is the leading infectious cause of blindness worldwide initiated by repeated infections of the conjunctiva with Chlamydia trachomatis (Ct). Consequent chronic inflammation results in formation of fibrotic tissue which can eventually lead to damage of the corneal surface. Lately, emerging evidence suggests involvement of epithelial to mesenchymaltransition (EMT) in development of every fibrotic process. Process of EMT represents reversible transition of epithelial into mesenchymalcells that can be traced through numerous markers such as E-cadherin, highly expressed in epithelial cells, α-SMA, a frequently used mesenchymalEMT marker and fibronectin, a glycoprotein whose expression increases during the EMT process. Recent findings have suggested that epigenetic events are master regulators of expression of many EMT related genes. Poster session presented at: CoMBoS. 1st Congress of Molecular Biologists of Serbia; 2017 Sep 20-22; Belgrade, Serbia. (P48-MBE)

A search for a signal consistent with the type-III seesaw mechanism in events with three or more electrons or muons is presented. The data sample consists of proton-proton collisions at root s = 13 TeV collected by the CMS experiment at the LHC in 2016 and corresponds to an integrated luminosity of 35.9 fb(-1). Selection criteria based on the number of leptons and the invariant mass of oppositely charged lepton pairs are used to distinguish the signal from the standard model background. The observations are consistent with the expectations from standard model processes. The results are used to place limits on the production of heavy fermions of the type-III seesaw model as a function of the branching ratio to each lepton flavor. In the scenario of equal branching fractions to each lepton flavor, heavy fermions with masses below 840 GeV are excluded. This is the most sensitive probe to date of the type-III seesaw mechanism.

A search is presented for massive spin-1 Z resonances decaying to a top quark and a heavy vector-like top quark partner T. The search is based on a 2.6 fb(-1) sample of proton-proton collisions at 13TeV collected with the CMS detector at the LHC. The analysis is optimized for final states in which the T quark decays to a W boson and a bottom quark. The focus is on all-jet final states in which both the W boson and the top quark decay into quarks that evolve into jets. The decay products of the top quark and of the W boson are assumed to be highly Lorentz-boosted and cannot be reconstructed as separate jets, but are instead reconstructed as merged, wide jets. Techniques for the identification of jet substructure and jet flavour are used to distinguish signal from background events. Several models for Z bosons decaying to T quarks are excluded at 95% confidence level, with upper limits on the cross section ranging from 0.13 to 10 pb, depending on the chosen hypotheses. This is the first search for a neutral spin-1 heavy resonance decaying to a top quark and a vector-like T quark in the all-hadronic final state.

The WZ production cross section is measured by the CMS experiment at the CERN LHC in proton proton collision data samples corresponding to integrated luminosities of 4.9 fb(-1) collected at root s = 7 TeV, and 19.6fb(-1) at root s = 8 TeV. The measurements are performed using the fully-leptonic WZ decay modes with electrons and muons in the final state. The measured cross sections for 71 LT m(Z) LT 111 GeV are sigma (pp - GT WZ; root s = 7 TeV) = 20.14 +/- 1.32 (stat)+/- 0.38 (theo)+/- 1.06 (exp)+/- 0.44 (lumi) pb and sigma (pp - GT WZ; root s = 8 TeV) = 24.09 +/- 0.87 (stat) 0.80 (theo) +/- 1.40 (exp) +/- 0.63 (lumi) pb. Differential cross sections with respect to the Z boson p(T), the leading jet p(T), and the number of jets are obtained using the root s = 8 TeV data. The results are consistent with standard model predictions and constraints on anomalous triple gauge couplings are obtained.

A search for physics beyond the standard model in final states with at least one photon, large transverse momentum imbalance, and large total transverse event activity is presented. Such topologies can be produced in gauge-mediated supersymmetry models in which pair-produced gluinos or squarks decay to photons and gravitinos via shortlived neutralinos. The data sample corresponds to an integrated luminosity of 35.9 fb(-1) of proton-proton collisions at root s = 13TeV recorded by the CMS experiment at the LHC in 2016. No significant excess of events above the expected standard model background is observed. The data are interpreted in simplified models of gluino and squark pair production, in which gluinos or squarks decay via neutralinos to photons. Gluino masses of up to 1.50-2.00TeV and squark masses up to 1.30-1.65TeV are excluded at 95% confidence level, depending on the neutralino mass and branching fraction.

A search for a light pseudoscalar Higgs boson (A) produced in association with bottom quarks and decaying into a muon pair is reported. The search uses 19.7 fb(-1) of proton-proton collisions at a center-of-mass energy of 8TeV, collected by the CMS experiment. No signal is observed in the dimuon mass range from 25 to 60 GeV. Upper limits on the cross section times branching fraction, sigma(pp - GT b $(b) over bar $A) B(A - GT mu mu), are set.

A data sample of events from proton-proton collisions with two isolated same-sign leptons, missing transverse momentum, and jets is studied in a search for signatures of new physics phenomena by the CMS Collaboration at the LHC. The data correspond to an integrated luminosity of 35.9 fb(-1), and a center-of-mass energy of 13 TeV. The properties of the events are consistent with expectations from standard model processes, and no excess yield is observed. Exclusion limits at 95% confidence level are set on cross sections for the pair production of gluinos, squarks, and same-sign top quarks, as well as top-quark associated production of a heavy scalar or pseudoscalar boson decaying to top quarks, and on the standard model production of events with four top quarks. The observed lower mass limits are as high as 1500 GeV for gluinos, 830 GeV for bottom squarks. The excluded mass range for heavy (pseudo) scalar bosons is 350-360 (350-410) GeV. Additionally, model-independent limits in several topological regions are provided, allowing for further interpretations of the results.

A search is performed for Higgs-boson-mediated flavor-changing neutral currents in the decays of top quarks. The search is based on proton-proton collision data corresponding to an integrated luminosity of 19.7 fb(-1) at a center-of-mass energy of 8 TeV collected with the CMS detector at the LHC. Events in which a top quark pair is produced with one top quark decaying into a charm or up quark and a Higgs boson (H), and the other top quark decaying into a bottom quark and a W boson are selected. The Higgs boson in these events is assumed to subsequently decay into either dibosons or difermions. No signi fi cant excess is observed above the expected standard model background, and an upper limit at the 95% con fi dence level is set on the branching fraction B (t - GT Hc) of 0.40% and B (t - GT Hu) of 0.55%, where the expected upper limits are 0.43% and 0.40%, respectively. These results correspond to upper limits on the square of the flavor-changing Higgs boson Yukawa couplings vertical bar lambda(H)(tc)vertical bar(2) LT 6 . 9 x 10(-3) and vertical bar lambda(H)(tu)vertical bar(2) LT 9 . 8 x 10(-3).

Results are reported from a search for supersymmetric particles in protonproton collisions in the final state with a single, high transverse momentum lepton; multiple jets, including at least one b-tagged jet; and large missing transverse momentum. The data sample corresponds to an integrated luminosity of 2: 3 fb(-1) at root s = 13TeV, recorded by the CMS experiment at the LHC. The search focuses on processes leading to high jet multiplicities, such as gluino pair production with (g) over tilde - GT t (t) over bar(chi) over tilde (0)(1) . The quantity M-J, defined as the sum of the masses of the large-radius jets in the event, is used in conjunction with other kinematic variables to provide discrimination between signal and background and as a key part of the background estimation method. The observed event yields in the signal regions in data are consistent with those expected for standard model backgrounds, estimated from control regions in data. Exclusion limits are obtained for a simplified model corresponding to gluino pair production with three-body decays into top quarks and neutralinos. Gluinos with a mass below 1600 GeV are excluded at a 95% confidence level for scenarios with low (chi) over tilde (0)(1) mass, and neutralinos with a mass below 800 GeV are excluded for a gluino mass of about 1300 GeV. For models with two-body gluino decays producing on-shell top squarks, the excluded region is only weakly sensitive to the top squark mass.

A measurement of the cross section for top quark-antiquark (t (t) over bar) pairs produced in association with a photon in proton-proton collisions at root s = 8TeV is presented. The analysis uses data collected with the CMS detector at the LHC, corresponding to an integrated luminosity of 19.7 fb(1). The signal is de fined as the production of a t (t) over bar pair in association with a photon having a transverse energy larger than 25 GeV and an absolute pseudorapidity smaller than 1.44. The measurement is performed in the fiducial phase space corresponding to the semileptonic decay chain of the t (t) over bar pair, and the cross section is measured relative to the inclusive t (t) over bar pair production cross section. The fiducial cross section for associated t (t) over bar pair and photon production is found to be 127 +/- 27 (stat + syst) fb per semileptonic final state. The measured value is in agreement with the theoretical prediction.

A measurement of the top quark mass is reported in events containing a single top quark produced via the electroweak t channel. The analysis is performed using data from proton-proton collisions collected with the CMS detector at the LHC at a centre-of-mass energy of 8 TeV, corresponding to an integrated luminosity of 19.7 fb(-1). Top quark candidates are reconstructed from their decay to a W boson and a b quark, with the W boson decaying leptonically to a muon and a neutrino. The final state signature and kinematic properties of single top quark events in the t channel are used to enhance the purity of the sample, suppressing the contribution from top quark pair production. A fit to the invariant mass distribution of reconstructed top quark candidates yields a value of the top quark mass of 172.95 +/- 0.77 (stat)(-0.93)(+0.97) (syst) GeV. This result is in agreement with the current world average, and represents the first measurement of the top quark mass in event topologies not dominated by top quark pair production, therefore contributing to future averages with partially uncorrelated systematic uncertainties and a largely uncorrelated statistical uncertainty.

Normalized double-differential cross sections for top quark pair (t (t) over bar) production are measured in pp collisions at a centre-of-mass energy of 8 TeV with the CMS experiment at the LHC. The analyzed data correspond to an integrated luminosity of 19.7 fb(-1). The measurement is performed in the dilepton e(+/-)mu(+/-) final state. The tt cross section is determined as a function of various pairs of observables characterizing the kinematics of the top quark and tt system. The data are compared to calculations using perturbative quantum chromodynamics at next-to-leading and approximate next-to-next-to-leading orders. They are also compared to predictions of Monte Carlo event generators that complement fixed-order computations with parton showers, hadronization, and multiple-parton interactions. Overall agreement is observed with the predictions, which is improved when the latest global sets of proton parton distribution functions are used. The inclusion of the measured tt cross sections in a fit of parametrized parton distribution functions is shown to have significant impact on the gluon distribution.

Introduction: Since diabetes is characterized by impaired ability of pancreatic betacells to respond and/or produce insulin, new approaches for renewal and replacement of deficient beta-cells are indispensable. The aim of this study is direct pancreatic alpha- to beta-cells transdifferentiation by using a new synthetic epigenetic tool, Epi-CRISPR system. Using Epi-CRISPR system we aim to introduce targeted DNA methylation and subsequent repression of genes responsible for maintaining alpha-cell identity. Methods: AlphaTC1-6 cells (α-cells) were transiently transfected with dCas9-Dnmt3a- Dnmt3L constructs and one or four different vectors containing guide RNA components for specific targeting the promoter region of aristaless-related homeobox gene (Arx). The success of α-cells transdifferentiation into insulinproducing cells was evaluated by measuring Arx and insulin mRNA level, amount of secreted insulin and by immunostaining of insulin/glucagon in the cells. Results: We observed Arx transcriptional repression in α-cell transfected with Epi- CRISPR construct that targets the Arx gene promoter inducing subsequent methylation. At fifth day post-transfection the expression of Arx was decreased in α- cells followed by consequent increase in insulin (mRNA and protein level). At the same time, the glucagon levels remained unchanged. At twelfth day posttransfection the transfected cells start to lose glucagon while still secreting insulin. Conclusion: This study is near to confirm Epi-CRISPR system functionality and to verify the concept of cell transdifferentiation through silencing of genes responsible for maintaining cell phenotype. The obtained results will be valuable for later Epi- CRISPRs use in mouse in vivo models of diabetes and eventually as a future therapy for diabetes attenuation in humans. Brajušković G, Đorđević A, editors. CoMBoS. 1st Congress of Molecular Biologists of Serbia; 2017 Sep 20-21; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Biology; 2017. p. 73.

Single top quark events produced in the t channel are used to set limits on anomalous Wtb couplings and to search for top quark flavour-changing neutral current (FCNC) interactions. The data taken with the CMS detector at the LHC in proton-proton collisions at and 8 TeV correspond to integrated luminosities of 5.0 and 19.7 fb(-1), respectively. The analysis is performed using events with one muon and two or three jets. A Bayesian neural network technique is used to discriminate between the signal and backgrounds, which are observed to be consistent with the standard model prediction. The 95% confidence level (CL) exclusion limits on anomalous right-handed vector, and left- and right-handed tensor Wtb couplings are measured to be vertical bar f (V) (R) vertical bar LT 0.16,aEuro,vertical bar f (T) (L) vertical bar LT 0.057, and - 0.049 LT f (T) (R) LT 0.048, respectively. For the FCNC couplings kappa (tug) and kappa (tcg), the 95% CL upper limits on coupling strengths are vertical bar kappa (tug)vertical bar/I GT LT 4.1 x 10(- 3) TeV-1 and vertical bar kappa (tcg)vertical bar/Lambda LT 1.8 x 10(- 2) TeV-1, where I GT is the scale for new physics, and correspond to upper limits on the branching fractions of 2.0 x 10(-5) and 4.1 x 10(-4) for the decays t - GT ug and t - GT cg, respectively.

An inclusive search is performed for supersymmetry in final states containing jets and an apparent imbalance in transverse momentum, (p) over right arrow (miss)(T),due to the production of unobserved weakly interacting particles in pp collisions at a centre-of-mass energy of 8 TeV. The data, recorded with the CMS detector at the CERN LHC, correspond to an integrated luminosity of 18.5fb(-1). The dimensionless kinematic variable alpha(T) is used to discriminate between events with genuine (p) over right arrow (miss)(T)associated with unobserved particles and spurious values of (p) over right arrow (miss)(T) Tarising from jet energy mismeasurements. No excess of event yields above the expected standard model backgrounds is observed. The results are interpreted in terms of constraints on the parameter space of several simplified models of supersymmetry that assume the pair production of top squarks. The search provides sensitivity to a broad range of top squark ((t) over tilde) decay modes, including the two-body decay (t)over tilde GT - GT c (chi) over tilde (0)(1),where c is a charm quark (chi) over tilde (0)(1) and is the lightest neutralino, as well as the four-body decay (t)over tilde GT - GT bf (f) over bar (chi) over bar (0)(1),where b is a bottom quark and f and (f) over bar are fermions produced in the decay of an intermediate off-shell W boson. These modes dominate in scenarios in which the top squark and lightest neutralino are nearly degenerate in mass. For these modes, top squarks with masses as large as 260 and 225 GeV are excluded, respectively, for the two-and four-body decays. (C) 2017 The Author. Published by Elsevier B.V.

The results of a first search for CP violation in the production and decay of top quark-antiquark (t (t) over bar) pairs are presented. The search is based on asymmetries in T-odd, triple-product correlation observables, where T is the time-reversal operator. The analysis uses a sample of proton-proton collisions at root s = 8 TeV collected by the CMS experiment, corresponding to an integrated luminosity of 19.7fb(-1). Events are selected having one electron or muon and at least four jets. The T-odd observables are measured using four-momentum vectors associated with t (t) over bar production and decay. The measured asymmetries exhibit no evidence for CP-violating effects, consistent with the expectation from the standard model.

Cross sections for the production of a Z boson in association with jets in proton-proton collisions at a centre-of-mass energy of root s = 8 TeV are measured using a data sample collected by the CMS experiment at the LHC corresponding to 19.6 fb(-1). Differential cross sections are presented as functions of up to three observables that describe the jet kinematics and the jet activity. Correlations between the azimuthal directions and the rapidities of the jets and the Z boson are studied in detail. The predictions of a number of multileg generators with leading or next-to-leading order accuracy are compared with the measurements. The comparison shows the importance of including multi-parton contributions in the matrix elements and the improvement in the predictions when nextto- leading order terms are included.

A search is presented for decays beyond the standard model of the 125 GeV Higgs bosons to a pair of light bosons, based on models with extended scalar sectors. Light boson masses between 5 and 62.5 GeV are probed in final states containing four tau leptons, two muons and two b quarks, or two muons and two tau leptons. The results are from data in proton-proton collisions corresponding to an integrated luminosity of 19.7 fb(-1), accumulated by the CMS experiment at the LHC at a center-of-mass energy of 8TeV. No evidence for such exotic decays is found in the data. Upper limits are set on the product of the cross section and branching fraction for several signal processes. The results are also compared to predictions of two-Higgs-doublet models, including those with an additional scalar singlet.

A measurement of the t (t) over bar production cross section at root s = 13 TeV is presented using proton-proton collisions, corresponding to an integrated luminosity of 2.2 fb(-1), collected with the CMS detector at the LHC. Final states with one isolated charged lepton (electron or muon) and at least one jet are selected and categorized according to the accompanying jet multiplicity. From a likelihood fit to the invariant mass distribution of the isolated lepton and a jet identified as coming from the hadronization of a bottom quark, the cross section is measured to be sigma(tt) = 888 +/- 2; (stat) (+26)(-28) (syst) +/- 20 (lumi) pb, in agreement with the standard model prediction. Using the expected dependence of the cross section on the pole mass of the top quark (m(t)), the value of m(t) is found to be 170:6 +/- 2:7 GeV.