1. Paper-based immune-affinity arrays for detection of multiple mycotoxins in cereals
- Author
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Xizhi Jiang, Liru Xia, Hongpu Chen, Yongfu Zhao, Li Li, He-Ye Wang, Yifei Jiang, Xiaolan Lv, and Min Wang
- Subjects
Paper ,Food Contamination ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Polymerization ,chemistry.chemical_compound ,Limit of Detection ,medicine ,Animals ,Humans ,Mycotoxin ,Zearalenone ,Immunoassay ,Chromatography ,Toxin ,010401 analytical chemistry ,technology, industry, and agriculture ,food and beverages ,Reproducibility of Results ,Paper based ,Equipment Design ,Mycotoxins ,0104 chemical sciences ,T-2 Toxin ,chemistry ,Luminescent Measurements ,Edible Grain ,Trichothecenes ,Antibodies, Immobilized ,Food Analysis - Abstract
Mycotoxins produced by different species of fungi may coexist in cereals and feedstuffs, and could be highly toxic for humans and animals. For quantification of multiple mycotoxins in cereals, we developed a paper-based mycotoxin immune-affinity array. First, paper-based microzone arrays were fabricated by photolithography. Then, monoclonal mycotoxin antibodies were added in a copolymerization reaction with a cross-linker to form an immune-affinity monolith on the paper-based microzone array. With use of a competitive immune-response format, paper-based mycotoxin immune-affinity arrays were successfully applied to detect mycotoxins in samples. The detection limits for deoxynivalenol, zearalenone, T-2 toxin, and HT-2 toxin were 62.7, 10.8, 0.36, and 0.23 μg·kg-1, respectively, which meet relevant requirements for these compounds in food. The recovery rates were 81–86% for deoxynivalenol, 89–117% for zearalenone, 79–86% for T-2 toxin, and 78–83% for HT-2 toxin, and showed the paper-based immune-affinity arrays had good reproducibility. In summary, the paper-based mycotoxin immune-affinity array provides a sensitive, rapid, accurate, stable, and convenient platform for detection of multiple mycotoxins in agro-foods.
- Published
- 2017