1. Cellulolytic enzyme system of Acetivibrio cellulolyticus
- Author
-
Saddler Jn and Khan Aw
- Subjects
Cellobiose ,Glycoside Hydrolases ,Immunology ,Polyacrylamide ,Cellulase ,Biology ,Applied Microbiology and Biotechnology ,Microbiology ,Substrate Specificity ,chemistry.chemical_compound ,Enzyme system ,Genetics ,medicine ,Cellulose 1,4-beta-Cellobiosidase ,Cellulose ,Molecular Biology ,Polyacrylamide gel electrophoresis ,chemistry.chemical_classification ,Gram-Negative Anaerobic Bacteria ,Molecular mass ,beta-Glucosidase ,Acetivibrio cellulolyticus ,General Medicine ,Carboxymethyl cellulose ,Molecular Weight ,Enzyme ,chemistry ,Biochemistry ,biology.protein ,Electrophoresis, Polyacrylamide Gel ,Glucosidases ,medicine.drug - Abstract
Polyacrylamide gel electrophoresis of the cellulolytic system from culture supernates of Acetivibrio cellulolyticus showed the presence of four major enzymes: a β-glucosidase, an exoglucanase, and two endoglucanases. The relative proportions of these enzymes in the culture supernate were affected by the nature of the cellulosic substrate and by the length of the incubation period. The molecular weights of the cellulolytic enzymes were β-glucosidase, 81 000; exoglucanase, 38 000; endoglucanase C2, 33 000; and endoglucanase C3, 10 400, as estimated by their electrophoretic mobilities relative to proteins of known molecular weight. Treatment of the high molecular weight endoglucanase with SDS–mercaptoethanol led to reversible dissociation of the enzyme into polypeptide subunits similar to the low molecular weight endoglucanase. Endoglucanase activity could be assayed for directly using a novel method of incorporating carboxymethyl cellulose in the polyacrylamide gels. The molecular weights and functions of these enzymes are compared with those detected in culture filtrates of various fungi.
- Published
- 1981