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This study aims to inspect the effects of Imidacloprid (IMI) acute toxicity in the ovaries of the red palm weevil (Rhynchophorus ferrugineus). The LC50 of IMI was determined to be 15.7 ppm. We assessed the biochemical changes resulting from treatment with four IMI concentrations (10, 15, 20 and 30 ppm). IMI reduced acetylcholine esterase activity in a dose-dependent manner. Glutathione (GSH) content decreased with increasing concentration of IMI; however, GSH was not affected and accompanied by an increase in catalase activity with the highest concentration of IMI. Lipid peroxidation increased steadily as IMI concentration increased. Based on the ultrastructural analyses of the follicle cells and the ooplasm of developing oocytes, acute IMI toxicity produced damaged yolk bodies, pale nuclei, and shrinking microvilli. Fractionation of rough endoplasmic reticulum, deformed mitochondria, and autophagic bodies were also observed. In conclusion, these ovarian pathobiochemical changes could be used as biomarkers of acute IMI exposure.

The current study aims for the use of the solid-state technique as an efficient way for the preparation of zinc oxide nanoparticles (ZnONPs) as an antimicrobial agent with high concentration using sodium alginate as stabilizing agent. ZnONPs were prepared with three different concentrations: ZnONPs-1, ZnONPs-2, and ZnONPs-3 (attributed to the utilized different concentrations of zinc acetate, 1.5, 3 and 4.5 g, respectively). The as-fabricated ZnONPs (ZnONPs-1, ZnONPs-2, and ZnONPs-3) were used for the treatment of cellulosic fabrics as dressing materials for the diabetic wounds. DLS findings illustrated that the as-prepared ZnONPs exhibited average particle size equal to 78, 117, and 144 nm, respectively. The data also showed that all the formulated ZnONPs were formed with good stability (above −30 mv). The topographical images of cellulosic fabrics loaded with ZnONPs that were obtained by SEM confirmed the deposition of nanoparticles onto the surface of cellulosic fabrics with no noticeable agglomeration. The findings also outlined that the treated cellulosic fabrics dressings were proven to have enhanced bactericidal characteristics against the pathogenic microorganisms. The finding of wound contraction for the diabetic rats was measured after 21 days and reached 93.5% after treating the diabetic wound with cotton fabrics containing ZnONPs-2. Ultimately, the generated wound dressing (cellulosic fabrics loaded with ZnONPs) offers considerable promise for treating the wound infections and might be examined as a viable alternative to antibiotics and topical wound treatments.

The use of green nano-amendments is a promising approach for improving soil health and providing sustainable options to reduce root-knot nematodes (RKN) and thus increase yields. Therefore, the purpose of this research was to identify the characteristics of nano-amendments such as nanobiochar (nB), green nanobiochar (GnB), and magnetic nanobiochar (MnB) and their effect on the root-knot nematodes and tomato yield at levels of 3 and 6 mg kg−1 in sandy loam soil. The results showed that the GnB and MnB contain many functional groups (such as O-H, C=C, S-H, H-C=O, C-O, and H–O–H) and minerals (such as magnetite, ferrous sulfate monohydrate, and quartz), and they also had an elevated specific surface area. The application of the investigated soil nano-amendments significantly increased soil organic matter (OM) and microbial biomass carbon (MBC) and decreased the root-knot nematodes, playing a major role in increasing tomato growth. The highest significant values of OM and MBC were found in the soil amended by GnB at 6 mg kg−1, with increases of 84.7% and 71.5% as compared to the control, respectively. GnB6 significantly decreased the number of root galls, the egg mass, and number of nematodes per 250 cm3 soil by 77.67, 88.65, and 74.46%, respectively, compared to the control. Green nanobiochar was more efficient in accelerating the growth and yield components of the tomato plant. The addition of GnB is an effective strategy and an environmentally friendly technology to control plant parasitic nematodes and increase tomato yield. Therefore, the results recommend adding GnB at a rate of 6 mg kg−1 in sandy loam soil.

Recycling waste, such as rice straw and water treatment residuals, is important to reduce harmful effects on the environment and to improve canola yield and soil quality in degraded soils. Nanotechnology for the production of nanomaterials from biochar and water treatment residues will be a future revolution for improving soil quality and increasing canola yield in degraded soil. Therefore, this study aims to identify the properties of some recycled nanomaterials, such as nanobiochar (nB) and nanowater treatment residue (nWTR), and their effect on the biological activity and productivity of canola in degraded soils. The results showed that the nWTR and nB contain many functional groups and minerals, and they also have high negative zeta potential. The addition of the studied soil amendments significantly improved microbial biomass carbon (MBC) and biological activity, which played a major role in increasing canola yield. The highest dehydrogenase (DHA) and catalase (CLA) activity was found in nWTR-treated soil at 50 mg kg−1, with increases of 32.8% and 566.7% compared to the control, respectively. The addition of nB greatly improved the growth of canola plants in the soil. This was evident from the increase in the weight of seeds, the weight of 1000 grains, the number of pods per plant, and the highest increase was for nB added at the rate of 250 mg per kg−1 soil. The addition of 50 mg kg−1 of nWTR gave the best results in seed yield by 150.64% compared to the control. These results indicate that recycled nWTR and nB are some of the best waste recycling treatments, in addition to good soil health, in increasing soil biology and canola yield in degraded soils. In the future, research on recycled nanomaterials should examine the residual effect they have on yield, soil quality, and soil fauna in the long term.

Rutin, a food derived-polyphenolic bioflavonoid, has been acknowledged for several health benefits. This study aims to explore the ameliorative effects of rutin against carbon tetrachloride (CCl4) toxicity in male rats. Adult male rats were given either CCl4 (30% in olive oil, 3 ml/kg b.w. intraperitoneally) alone or in combination with rutin (70 mg/kg intragastrically) twice a week for 4 weeks. Our data showed that rutin mitigated CCl4 hepatorenal damage, as indicated by diagnostic markers (i.e., transaminases, alkaline phosphatase, total bilirubin, total protein, albumin, urea, uric acid and creatinine), and histopathological findings. In addition, CCl4 induced profound elevation of free radical generation and oxidative stress, as evidenced by increasing lipid peroxidation and reducing catalase, superoxide dismutase and glutathione peroxidase activities in liver, kidney and testicular tissues; these effects were suppressed by coexposure with rutin. Moreover, the increase in the levels of serum triglycerides, cholesterol, low-density lipoprotein cholesterol, and very-low-density lipoprotein cholesterol induced by CCl4 was effectively counteracted by rutin. The decrease in the level of high-density lipoprotein cholesterol in the CCl4 group was also counteracted by rutin treatment. Interestingly, the decreased levels of hormonal mediators associated with sperm production, including serum testosterone, luteinizing hormone and follicle-stimulating hormone, and the impaired sperm quality induced by CCl4 were reversed by rutin. Data from the current study clearly demonstrated that rutin supplementation could at least partly overcome CCl4-induced hepatotoxicity, nephrotoxicity and reproductive toxicity by antioxidant and antidyslipidemic effects.