Search

Tibetisoperla elongata sp. nov. urn:lsid:zoobank.org:act: 7B5313C1-F26C-41C5-88BD-7B0486372A08 Figs 1–4 Diagnosis General color brownish. Vesicle developed and tongue-shaped, which is almost as long as its width. Aedeagus mostly membranous, except for a v-shaped ventrobasal sclerite, generally tubular and slightly curved ventrally at apex. Subgenital plate in female produced and subquadrate, posteriorly with a broad arcuate posteromedial notch. Etymology The specific epithet refers to the vesicle being long, which was presumed to be atypical for this genus. Material examined Holotype CHINA • ♂; Qinghai Province, Menyuan County, Daitong River; 37°21′37.99″ N, 101°36′55.30″ E; alt. 2782 m; 30 Jun. 2021; Wei-Hai Li and Rong-Rong Shen leg.; HIST. Paratypes CHINA • 1 ♂; same collection data as for holotype; HIST • 3 ♀♀; same collection data as for holotype; HIST • 1 ♂; same location as for holotype; 37°18′1.38″ N, 101°55′49.82″ E; alt. 2643 m; 26 Jul. 2021; Wei-Hai Li and Bin Zhang leg.; HIST. Description Male ADULT HABITUS (Figs 1A, 1C). Forewing length 8.1–8.5 mm, hindwing length 7.0– 7.2 mm (N=3). Head brown with dark brown arween ocelli and tentorial callosities. M-line dark brown. Triocellate, anterior ocellus smaller. Antennae brownish, scape brown, palpi brownish. Pronotum disc with large area of brown to dark brown rugosities and pale medial stripe. Legs brownish. Wings hyaline, veins brown. ABDOMEN (Figs 1D, 2). Abdominal terga brownish and terga 1–4 darkly sclerotized. Terga 2–9 each with a pair of small dark spots medially and dark medial strip (obscure in young individual). Tergum 9 bearing two paramedial patches of sensilla on darker posterior margin, patches separated by pale T-shaped mesal membrane. Tergum 10 bilobate, distally upcurved with 3–4 thick apical setae. Vesicle located on posterior margin of sternum 8, generally tongue-shaped, ca almost as long as its width, basally brown but apex paler, covered with hairs. Paraprocts sclerotized, with partially separated apical sclerite covered by small terminal spines. AEDEAGUS (Fig. 3). Membranous except for a v-shaped ventrobasal sclerite, tubular and slightly curved ventrally at apex, mostly covered by fine spinules, dorsoapical and ventroapical portions bald. Female ADULT HABITUS (Fig. 1B). Forewing length 9.0– 9.9 mm, hindwing length 7.9–8.2 mm. Body coloration similar to male. ABDOMEN (Fig. 4). Tergum 10 unmodified. Subgenital plate sclerotized and subquadrate, produced posteriorly covering anterior half of sternum 9, with a broad arcuate posteromedial notch. EGG. Unknown. Distribution China: Qinghai Province. Presently only known from two sites along the riverside of Daitong River in Menyuan County. Remarks The type species of Tibetisoperla, T. wangluyui Huo & Du, 2021 is a dark species and has a reduced vesicle on sternum 8, but the new species is a brownish species and has a developed vesicle which is present in typical Isoperla Banks, 1906 (Teslenko & Zhiltzova 2009; Murányi 2011; Szczytko & Kondratieff 2015; Cao et al. 2020), another genus of Isoperlinae in China. However, the large apical setae of tergum 10 and spiny apical sclerite of paraproct clearly show its placement in Tibetisoperla. The female of T. elongata is different to T. wangluyui in subgenital plate structure, the former is broadly semicircular while the latter is subquadrate. Additionally, T. elongata with a typically posterior notch which is indistinct of T. sclerotica. Terga 1–8 of female with black medial stripes and subgenital plate is broadly triangular in T. sclerotica., Published as part of Yan, Yan-Hua, Chen, Zhen-Ning & Bozdoğan, Hakan, 2022, The species of Tibetisoperla (Plecoptera: Perlodidae), pp. 125-140 in European Journal of Taxonomy 823 on pages 126-130, DOI: 10.5852/ejt.2022.823.1821, http://zenodo.org/record/6658611, {"references":["Huo Q. B. & Du Y. Z. 2021. A new genus of Isoperline (Plecoptera: Perlodidae) from Tibet, China. Zootaxa 4996 (2): 343 - 352. https: // doi. org / 10.11646 / zootaxa. 4996.2.8","Banks N. 1906. On the perlid genus Chloroperla. Entomological News 17: 174 - 175.","Teslenko V. A. & Zhiltzova L. A. 2009. Key to the Stoneflies (Insecta, Plecoptera) of Russia and Adjacent Countries. Imagines and Nymphs. Russian Academy of Sciences, Far Eastern Branch, Dalnauka, Vladivostok.","Muranyi D. 2011. Balkanian species of the genus Isoperla Banks, 1906 (Plecoptera: Perlodidae). Zootaxa 3049: 1 - 46. https: // doi. org / 10.1643 / CE- 08 - 219","Szczytko S. W. & Kondratieff B. C. 2015. A Review of the Eastern Nearctic Isoperlinae (Plecoptera: Perlodidae) with the description of twenty-two new species. Monographs of Illiesia 1: 1 - 289.","Cao Z. S., Wang Y. & Li W. H. 2020. A new species of Isoperla (Plecoptera: Perlodidae) from China. Zootaxa 4858 (2): 251 - 260. https: // doi. org / 10.11646 / zootaxa. 4858.2.6"]}

Tibetisoperla sclerotica sp. nov. urn:lsid:zoobank.org:act: 5F951431-4255-4A09-B0BB-A645800BB1B1 Figs 5–9 Diagnosis General color dark brown. Vesicle reduced to a black transverse band. Ventrobasal sclerite of aedeagus black, generally subquadrate in outline with a triangular postcentral notch. Etymology The specific epithet refers to the sclerotized ventrobasal sclerite of everted aedeagus. Material examined Holotype CHINA • ♂; Qinghai Province, Menyuan County, Meihua village; 37°16′6.99″ N, 102°8′3.58″ E; alt. 2979 m; 30 Jul. 2021; Wei-Hai Li leg.; HIST. Paratypes CHINA – Qinghai Province • 1 ♀; same locality as for holotype; 31 Jul. 2021; HIST • 2 ♀♀; Menyuan County, Xiami Town, Qihankaigou; 37°9′30.73″ N, 102°1′52.09″ E; alt. 2694 m; 3 Jul. 2021; Wei-Hai Li and Rong-Rong Shen leg.; HIST • 1 ♀; Menyuan County, Zhugu Village, Sigou; 37°7′54.39″ N, 102°23′57.56″ E; alt. 2570 m; 28 Jul. 2021; Bin Zhang leg.; HIST • 1 ♀; Menyuan County, Liuhuanggou; 37°47′45.24″ N, 101°14′53.16″ E; alt. 3407.8 m; 29 Jul. 2021; Zhuo-Yin Jiang leg.; HIST • 2 ♀♀; Qilian County, Zhamashenxigou, Dongchagou; 38°9′14.18″ N, 100°1′22.40″ E; alt. 3015 m; 9 Jul. 2021; Wei Zeng leg.; CAU. Description Male ADULT HABITUS (Figs 5A, C–D). General color dark brown to black (Fig. 5A). Head mostly brown, with dark area around ocelli, tentorial callosities and M-line, the area sometimes extending to center of occiput. M-line dark brown. Triocellate, anterior ocellus slightly smaller. Antennae brown, scape darker, palpi brownish. Pronotum disc with dark brown rugosities and wide, brown, longititudinal stripe disjunct with thin black midline. Legs brown, femora darker. Wings hyaline, veins brown. Cerci brownish, apical segments darker. ABDOMEN (Figs 5D, 6, 7A–B). Forewing length ca 7.1 mm, hindwing length ca 6.0 mm. Abdominal tergal pattern obscure due to entirely black coloration. Tergum 9 bearing two patches of black sensilla on posterior margin by triangular medial membranous area extended anterolaterally forming a T-shaped paler marking. Tergum 10 with thin, longitudinal, pale, medial line; distal half bilobate, with 4–5 apical setae. Vesicle located on posterior margin of sternum 8, reduced to black transverse band. Paraprocts with recurved spiny apical sclerite, covered by ca. 13–14 tiny spines. AEDEAGUS (Fig. 8). Mostly membranous except for a dark ventrobasal sclerite, sclerite subquadrate with triangular postcentral notch; greatly enlarged posterior to ventrobasal sclerite. Aedeagus apical third upcurved and generally globular before narrowest nipple-like apex, brownish; mostly covered by fine spinules but lacking large spine. Female ADULT HABITUS (Figs 5B, 9A). Forewing length 7.8–9.8 mm, hindwing length 5.8–8.0 mm (N=7). Body coloration generally similar to male. ABDOMEN (Fig. 9B–D). Abdominal segments lighter and terga 1–8 with black medial tergal stripes in young individuals. Tergum 10 unmodified. Sternum 8 with sclerotized lateral portion and posterior subgenital plate, anteromedial portion membranous. Subgenital plate broadly triangular with dense long hairs, covering anterior third of sternum 9, with a posteromedial notch; lateral margins partially separated and heavily sclerotized. Sternum 9 with a pair of slope paramedial strips. EGG. Unknown. Distribution China: Qinghai Province. Presently only known from Menyuan County. Remarks The new species closely resembles T. wangluyui Huo & Du, 2021 in dark body coloration and reduced vesicle on sternum 8, but it is easily separated from that species by the large ventrobasal sclerite of aedeagus which is entirely membranous in T. wangluyui.

State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan,China; yanzai1112@sina.com (Y.C.); fangdailongtwozero@126.com (D.-L.F.); yyde2013@163.com (Y.Y.);ymyzjk@163.com (J.-K.Z.); 13880286908@163.com (H.-L.L.); xb1990625@126.com (B.X.);caokaijin@163.com (Y.L.)

Foersteria xinjiangensis Yan & Chen, sp. nov. (Figure 1) Material examined. Holotype, ��, China, Xinjiang Prov., Hami, V���VI. 2002, Hongying Hu, No. 20036490. Paratype: 1 ♂, China, Xinjiang Prov., Hami, V���VI. 2002, Hongying Hu, No. 20036507. Holotype. Female, length of body 4.5 mm, of fore wing 4.2 mm. Head. Antenna as long as fore wing, with 29 segments, third segment 0.9 times as long as fourth segment, length of third, fourth and penultimate segments 3.2, 3.6 and 1.7 times their width, respectively, subapical segments comparatively robust, submoniliform; scape punctate. Length of maxillary palp 0.6 times height of head. POL: OD: OOL= 9: 6: 15. Occipital carina reduced ventrally and far removed from hypostomal carina. Frons slightly concave and largely coarsely punctate-rugose, with distinct a median carina. Length of eye in dorsal view 1.3 times temple. Vertex convex, punctate (Figure 1, g.). Temple subparallel-sided behind eyes, punctate (Figure 1, k.). Face very coarsely transversely rugose (Figure 1, h.). Clypeus transverse, flat, truncate apically, largely smooth and its apical rim not distinctly differentiated. Malar suture complete. Length of malar space almost equal to basal width of mandible. Mandible mainly rugose. Mesosoma. Length of mesosoma 1.3 times its height. Pronope deep, elliptical. Side of pronotum medially coarsely crenulate, ventrally and dorsally densely punctulate. Mesosternal suture rather deep, narrow and smooth (Figure 1, i.). Prepectal carina complete, strong. Precoxal sulcus absent. Mesopleuron largely smooth. Mesoscutum punctate. Notauli narrow, crenulate, without a medial carina posteriorly. Scutellar sulcus deep, wide, with one carina and several lateral crenulae. Scutellum convex and punctate. Surface of propodeum coarsely reticulaterugose. Wing. Fore wing, distance from apex of marginal cell of fore wing to apex of wing 0.5 times vein 1 -R 1. 1 -SR absent. r: 3 -SR+SR 1: 2 -SR= 9: 68: 19. 1 -M smoothly curved. SR 1 curved. cu-a postfurcal, oblique. 1 -CU 1 short. m-cu distinctly antefurcal, straight. Hind wing, 1 -M: 1 r-m= 27: 16. M+CU: 1 -M= 37: 23. cu-a weakly curved and subvertical. Legs. Hind legs of female missing. Fore coxa rugose. Tarsal claws simple, without acute lobe ventrally. Middle femur swollen, rugose ventrally, with a strongly longitudinal carina. Metasoma. Length of first tergite 0.5 times its apical width, its surface reticulate-rugose, slightly convex and dorsal carinae nearly reaching to apex of tergite. Second and third tergites densely reticulate-rugose. Epipleuron of metasomal tergites densely punctate, ventro-posterior rim of third tergite with fine striae. Medio-posterior of third tergite with transverse and longitudinal lamellate protrusions dorsally (Figure 1, j.). Colour. Black. Mandible yellow, except apex black. Palpi and tegulae yellowish brown. Pronotum ventrally, propodeum, dorsal surface of metasomal tergites and tarsi dark yellowish brown. Femora, tibiae and epipleuron of metasomal tergites yellowing brown. Trochanters, fore and middle femora dark reddish brown. Pterostigma brown, veins from brown to light brown, wing membrane pale brown. Male (Figure 1, a���f.). Antenna with 28 segments, length of third, fourth and penultimate segments 2.6, 3.0 and 1.3 times their width, respectively. Length of maxillary palp 0.8 times height of head. POL: OD: OOL= 9: 5: 13. Frons slightly concave and largely coarsely transversely striated medially, with distinct a median carina, laterally punctate-rugose. Length of eye in dorsal view 1.2 times temple. Pronope deep, nearly round. Side of pronotum medially and posteriorly coarsely crenulate, ventrally rugose, dorsally punctulate. Fore wing (Figure 1, c.), distance from apex of marginal cell of fore wing to apex of wing 0.4 times vein 1 -R 1. 1 -SR absent. r: 3 -SR+SR 1: 2 -SR= 7: 48: 11. Hind coxa largely rugose-punctate. Middle femur swollen, rugose ventrally, with a strongly longitudinal carina (Figure 1, f.). Hind femora swollen, rugose ventrally, with a longitudinal lamellate protrusion. Length of femur, tibia and basitarsus of hind leg 3.0, 8.3 and 4.6 times their width, respectively. Length of both hind tibial spurs 0.4 times basitarsus. Length of ovipositor sheath almost equal to fore wing, 4.7 times hind tibia. Pronotum ventrally, propodeum and dorsal surface of metasomal tergites black. Tibia and tarsi dark yellowish brown. Hind femur (except ventrally) yellowing brown. Diagnosis. Foersteria xinjiangensis sp. nov. is similar to F. laeviuscula Sz��pligeti, but differs in having the face very coarsely transversely rugose (the latter coarsely punctate); second tergite densely reticulate-rugose (the latter only finely punctate) and medio-posterior of third tergite with lamellate protrusions dorsally (the latter without lamellate protrusions dorsally). Biology. Unknown. Distribution. Known only from the type locality, Xinjiang Province, China. Etymology. It is named after the type locality of the species, Xinjiang Province, China., Published as part of Yan, Cheng-Jin, He, Jun-Hua & Chen, Xue-Xin, 2013, Two genera Foersteria Sz��pligeti, 1896 and Polydegmon Foerster, 1862 (Hymenoptera, Braconidae, Brachistinae) from China, with description of a new species, pp. 178-184 in Zootaxa 3683 (2) on pages 179-181, DOI: 10.11646/zootaxa.3683.2.6, http://zenodo.org/record/223255

Allonychiurus megasomus sp. nov. (Figures 1���17, Tables 1���2) Type material. Holotype: female, China: Jiangsu Province: Nanjing: Qingliangshan Park, 11.iv. 2006, collection number C 9459, collected by XIE Guilin and JIANG Jigang. Paratypes: 9 females and 1 juvenile male, same data as holotype. Deposited in School of Life Science, Nanjing University, China; 2 Paratypes in the Museum National d���Histoire Naturelle, Paris, France. Abd. V present absent present present present present seta m0 (after fig. 62, Rusek 1971) Ventral tube 8 + 8 6 + 6 6 + 6 8 + 8 ?? setae Description. Body length: Up to 2.1 mm, holotype 1.46 mm. Body colour: Milky white in alcohol. Pseudocelli (pso): formula as 32 / 133 / 33343 dorsally (Figs. 1 & 2), 11 /000/01110 ventrally, and 1 / 1 / 1 on subcoxae (Fig. 1). Parapseudocelli (psx): 1 + 1, only found on sternum of Abd. I, near base of ventral tube (Fig. 13). Sensilla (s): thick and blunt, formula as 2 /012/ 22222 dorsally (Figs. 1 & 2) and 2 /000/00000 ventrally (Fig. 11). Microsensilla (ms): tiny, thick and blunt, formula as 0/011/00000 dorsally (Fig. 1). Head: Antennae short and distinctly segmented, 0.6���0.7 times as long as head. Antennal base distinctly marked with granulation. Ratio of length of antennal segments I:II:III:IV=1.0:1.6���2.0:1.5���1.9:3.3���3.7. Ant. III sensory organ composed of 5 papillae, 5 guard setae, 2 small rods and 2 clubs; clubs mulberry-shaped. Lateral ms just behind sensory organ. Ant. IV subapical organite with distal part expanded to a globular shape, basolateral ms situated in posterior row of setae (Figs. 3���6). Anterior cephalic dorsal pso located in finely granulated area near antennal base. PAO composed of 18���22 secondarily granulated vesicles arranged in 2 rows along axis of organ (Fig. 7). Dorsal seta d0 present. 4 + 4 setae between two posterior inner pso, p 1 slightly anterior to others (Fig. 1). Labral setae 4 / 3, 4, 2 (Fig. 8). Labium AC type (Fjellberg 1999), papillae A, B, C, D, E respectively with 1, 4, 0, 3 and 4 guard setae (Fig. 9). Labial proximal setae 6, basomedian setae 4 (E, F, G, and f), basolateral setae 5 (b, c, d, e, e���) (D���Haese 2003) (Fig. 10). Postlabial setae 3 + 3 along ventral groove (Fig. 11). Body chaetotaxy: Body setae smooth, of different lengths and usually arranged symmetrically. Th. I���Abd. III dorsally without median setae. Th. I with 7���8 + 7���8 dorsal setae. Th. II���III and Abd. I���III respectively with 4 + 4 and 3 + 3 dorsal setae along median line. Abd. IV with dorsal median setae m0 and p0, Abd. V with m0, Abd. VI with a0 and p0 (Figs. 1 & 2). Th. I���III with 0+ 0, 1 + 1, 1 + 1 (2 + 2) ventral setae respectively between coxae. Subcoxa 1 with 4, 5, 5, subcoxa 2 with 1, 4, 4 setae respectively on legs I���III. Appendages: Tibiotarsal setae as 21 (11, 8, 2), 20 (11, 7, 2) and 19 (11, 7, 1) respectively on legs I���III; Unguis without teeth. Unguiculus very slender and pointed, without inner basal lamella, 0.6���0.7 as long as inner edge of unguis (Fig. 12). Ventral tube with 8 + 8 distal setae and no basal setae (Fig. 13). Furcula reduced to a finely granulated area, with 4 small setae in two rows present posterior furcal rudiment (Fig. 14). Genital plate consisting of 13 setae in male (preadult) and 17���21 in female (Figs. 15 & 16). Male ventral organ absent. Anal valves with numerous acuminate setae; each lateral valve with a0 and 2 a 1 setae; upper valve with a0, b 0, 2 b 1, 2 b 2, c 0, 2 c 1 and 2 c 2 (after Yoshii 1996) (Fig. 18). Anal spines stout, set on distinct papillae, about 0.8 times length of inner edge of hind unguis (Figs. 2 & 17). Ecology. Found under moist leaf and grass litter, stones and bricks in woods of deciduous trees and bamboo. Etymology. The name of the new species is derived from Greek megas and soma. It refers to the large size of the species. Remarks. The new species is diagnosed by the arrangement and number of pseudocelli, ventral parapseudocelli, dorsal sensilla, the form of the PAO, the chaetotaxy of Abd. IV and V, the number of setae on the tibiotarsus, the unguiculus without basal lamella and the number of setae on the ventral tube. It is similar to some Asian species, such as: shanghaiensis (Rusek, 1971), kimi (Lee, 1973), shinbugensis (Lee, 1974), jongaksanensis (Weiner, 1989), flavescens (Kinoshita, 1916) in the Ant. III sensory organ, dorsal sensilla, abdominal dorsal pso, median setae on Abd. V and VI. However, it is easily separated from them by the characters listed in the Table 1., Published as part of Sun, Xin, Yan, Haijuan & Chen, Jian-Xiu, 2009, A new species of the genus Allonychiurus from China (Collembola: Onychiuridae), pp. 61-68 in Zootaxa 2194 on pages 62-66, DOI: 10.5281/zenodo.189465, {"references":["Rusek, J. (1971) Zweiter Beitrag zur Kenntnis der Collembola (Apterygota) Chinas. Acta Entomologica Bohemoslovaca, 68 (2), 108 - 137.","Fjellberg, A. (1999) The labial palp in Collembola. Zoologischer Anzeiger, 237, 309 - 330.","Yoshii, R. (1996) Identity of some Japanese Collembola IV. \" Deuteraphorura \" Group of Onychiurus. Annals of the speleological research institute of Japan (Iwaizumi), 14, 1 - 15.","Lee, B. H. (1973) Etude de la faune Coreenne des Collemboles I. Liste des Collemboles de Coree et description de trois especes nouvelles. Revue d'Ecologie et de Biologie du Sol, 10 (3), 435 - 449.","Lee, B. H. (1974) Etude de la faune Coreenne des Insectes Collemboles III. Description de huit especes nouvelles de Neanuridae et Onychiuridae. Bulletin du Museum National d'Histoire Naturelle, Paris, Serie, 220 (3), 573 - 598.","Weiner, W. M. (1989) Onychiurinae (Onychiuridae, Collembola) of North Korea: species of the Paronychiurus flavescens (Kinoshita, 1916) group. Acta zoological cracoviensia, 32 (5), 85 - 92.","Kinoshita, S. (1916) On Japanese Collembola. Zoological Magazine (Tokyo), 337, 450 - 460."]}

Yan Chen,&ast; Shengyue Chen,&ast; Yuanyuan Han, Qing Xu, Xin Zhao Department of Cardiology, the Second Hospital of Dalian Medical University, Dalian, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Xin Zhao, Email zx81830@163.comAim: To investigate the role of neutrophil-to-lymphocyte ratio(NLR) and platelet-to-lymphocyte(PLR) in predicting the risk of in-hospital mortality in elderly acute myocardial infarction(AMI) patients.Methods: This study was a single-center, retrospective and observational study. From December 2015 to December 2021, a total of 1550 elderly patients (age ≥ 60 years) with AMI with complete clinical history data were enrolled in the Second Hospital of Dalian Medical University. Routine blood tests were performed on admission, and NLR and PLR were calculated based on neutrophil, platelet, and lymphocyte counts. Outcome was defined as all-cause mortality during hospitalization. Cox regression and restricted spline cubic(RCS) models were used to evaluate the association of NLR and in-hospital mortality risk and the association of PLR with in-hospital mortality risk, respectively.Results: (1) A total of 132 (8.5%) patients died during hospitalization. From the results of blood routine, the white blood cell, neutrophil, NLR and PLR in the death group were higher than those in the non-death group, while the lymphocyte was lower than that in the non-death group, and the difference was statistically significant (P < 0.05). (2) The results of receiver operating characteristic(ROC) curves analysis showed that the predictive ability of NLR (AUC = 0.790) for in-hospital death was better than that of PLR (AUC = 0.637). (3) Multivariate Cox proportional regression hazard models showed that high NLR was associated with the risk of in-hospital mortality in elderly AMI patients (HR = 3.091, 95% CI 2.097– 4.557, P < 0.001), while high PLR was not. (4) RCS models showed a nonlinear dose-response relationship between NLR and in-hospital death (P for nonlinear = 0.0007).Conclusion: High NLR (> 6.69) is associated with the risk of in-hospital mortality in elderly patients with AMI and can be an independent predictor of poor short-term prognosis in elderly patients with AMI.Keywords: neutrophil to lymphocyte, platelet-to-lymphocyte ratio, acute myocardial infarction, prognosis, restricted cubic splines

Herui Li,&ast; Yiming Ma,&ast; Zihang Zeng, Lijuan Luo, Tiao Li, Huihui Zeng, Yan Chen Department of Respiratory and Critical Care Medicine, the Second Xiangya Hospital, Central South University, Changsha, Hunan, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Yan Chen; Huihui Zeng, Department of Respiratory and Critical Care Medicine, the Second Xiangya Hospital, Central South University, 139 Renmin Road, Changsha, 410011, Hunan, People’s Republic of China, Email chenyan99727@csu.edu.cn; bonemarrow@csu.edu.cnBackground: Surgical and medical treatments are applied to pulmonary cryptococcosis (PC) in the real world, while the prognosis of different therapies is uncertain. This study investigated diagnosis, real-world therapy, follow-up outcomes, and prognosis factors, aiming to deepen our understanding of PC.Methods: Patients pathologically diagnosed with PC were retrospectively reviewed and followed up. Further comparisons and subgroup analyses were conducted in surgical and nonsurgical treatment individuals. Univariable and multivariable logistic regression methods were used to explore the risk factors associated with treatment failure.Results: One hundred and sixty-three patients were included in this study, of whom 92 underwent surgical removal of VATS or open lung surgery (68 of them received postoperative antifungal treatment) and 71 got antifungal drugs only. Compared with nonsurgical patients, surgical patients were more immunocompetent (73 [79.3%] cases vs 33 [46.5%]), showed milder symptoms and more limited pulmonary lesions. Although they had instant treatment response owing to lesions resection, there is no significant advantage in the rate of treatment failure. Multivariable regression showed independent predictive factors associated with treatment failure were polymorphonuclear (PMN)> 6.30&ast;109/L, albumin (Alb) < 40g/L and antifungal dosage < 400mg/d. Further analysis among patients with different immune statuses or symptoms demonstrated that sufficient antifungal dosage could reduce the rate of treatment failure.Conclusion: PC showed variable and nonspecific clinical features. PC patients with limited nodules/masses and mild symptoms often led to misdiagnosis and unnecessary lung resections. The potential risk factors including higher PMN and hypoalbuminemia could help clinicians to identify PC patients with poor treatment efficiency at an early stage. To note, sufficient antifungal dosage may improve the treatment outcomes.Keywords: pulmonary cryptococcosis, diagnosis, antifungal treatment, clinical features, surgery

China is one of the largest wheat production countries in the world. The wheat quality determines the price and many other aspects. The detection methods of wheat quality mainly depend on manual labor. It costs high amount of manpower and time, and the classification results are partly affected by different individuals. With the development of machine vision, an automatic classification system was presented in this study. A wheat unsound kernel identification method based on the improved YOLOv5 algorithm was designed by adding efficient channel attention (ECA). Compared with convolutional block attention module (CBAM) and squeeze-and-excitation network (SENet), the improved YOLOv5 algorithm was selected to fit the model better. The recognition results showed that YOLOv5 with the addition of the attention mechanism had a significant improvement in average accuracy over that without it. The most significant improvement was observed with the addition of ECA-YOLOv5, with an average accuracy of 96.24%, a 10% improvement over the other two models, and a 13% improvement over the original YOLOv5. This satisfied the application requirements for detection of wheat unsound kernel.

Recently, intelligent city construction has been promoted with the development of the Internet of things (IoT). The edge IoT gateway plays a critical role as the data aggregation core and processing center. Most existing gateways mainly solve heavy data storage and processing loads in cloud computing centers. There is less attention paid to multi-protocol data transmission and fusion. However, multiple products with different protocols in an IoT system require a flexible gateway compatible with multiple protocols. This paper proposes a multi-protocol edge gateway. The frame design was based on the actual demand for edge data acquisition. The gateway hardware platform used an RK3399 chip transplanted from the embedded operating system. It could support simultaneous multi-protocol access to ZigBee, LoRa, Bluetooth, and Wi-Fi. We combined the plug-and-play (PnP) hardware device access detection scheme with the system onboard interface driver to realize dynamic access detection and unified device management. In addition, the gateway also integrated data storage and access functions and partial edge computing functions. Finally, the experiment results verified that the multi-protocol edge gateway could meet the demand for data access and device control.

A direct oxidative alkylarylation reaction of N-arylacrylamides with simple alkanes for the synthesis of 3,3-disubstituted oxindoles under metal-free conditions was demonstrated. By using PhI(OAc)2 [(diacetoxy)iodobenzene] as an oxidant, a series of 3,3-disubstituted oxindoles bearing different aryl or alkyl substituents were generated in moderate to excellent chemical yields via a radical-initiated alkylation/cyclization process. The reported method features good functional group tolerance and wide substrate range, and provides an effective method for the preparation of various alkyl substituted 3,3-disubstituted oxindoles.

Species of the genus Protelenomus Kieffer (Platygastroidea, Scelionidae) are phoretic egg parasitoids of coreid bugs. The discovery, DNA sequencing, and molecular phylogenetic analysis of a Protelenomus species phoretic on Cletus punctiger (Dallas) (Hemiptera, Coreidae) shows that Protelenomus is a derived lineage of Trissolcus Ashmead. Protelenomus is treated as a junior synonym and a new species of phoretic Trissolcus, T. siliangae Yan, Chen & Talamas, is described from China.

We aimed to assess the value of dose distribution-based dosiomics and planning computed tomography-based radiomics to predict radiation-induced temporal lobe injury (TLI) and guide individualized intensity modulated radiation therapy.A total of 5599 nasopharyngeal carcinoma patients were enrolled, including 2503, 1072, 988, and 1036 patients in the training, validation, prospective test, and external test cohorts, respectively. The concordance index (C-index) was used to compare the performance of the radiomics and dosiomics models with that of the quantitative analyses of normal tissue effects in the clinic and Wen's models. The predicted TLI-free survival rates of redesigned simulated plans with the same dose-volume histogram but different dose distributions for same patient in a cohort of 30 randomly selected patients were compared by the Wilcoxon matched-pairs signed-rank test.The radiomics and dosiomics signatures were constructed based on 30 selected computed tomography features and 10 selected dose distribution features, respectively, which were important predictors of TLI-free survival (all P.001). However, the radiomics signature had a low C-index. The dosiomics risk model combining the dosiomics signature, DThe dosiomics risk model was superior to traditional models in predicting the risk of TLI. This is a promising approach to precisely predict radiation-induced toxicities and guide individualized intensity modulated radiation therapy.

The Global Initiative for Chronic Obstructive Lung Disease (GOLD) document suggests that patients with chronic obstructive pulmonary disease (COPD) should be divided into a less symptomatic group. Moreover, single-inhaled drugs are recommended as initial inhalation therapy for them. However, many less symptomatic patients are provided double or triple-inhaled drugs as initial therapy in the real world. This study aimed to describe the inhalation prescriptions and compare the effects of different inhalation therapies on less symptomatic COPD patients. This was an observational study. Stable COPD patients were recruited and divided into a less symptomatic group including Groups A and C based on the GOLD 2019 document. We collected the data of inhalation therapies prescriptions. Then, the patients were classified into long-acting muscarinic antagonist (LAMA), long-acting β2-agonist (LABA) + inhaled corticosteroid (ICS), LABA + LAMA, and LABA + LAMA + ICS groups. All the patients were followed up for 1 year to collect exacerbation and mortality data. We found that only 45.4% of patients in Group A and 43.6% of patients in Group C received reasonable inhalation therapy in reference to the GOLD document. In addition, the LAMA group had a higher forced expiratory volume in one second (FEV1), FEV1%pred, FEV1/forced vital capacity and peak expiratory flow compared with LABA + ICS, LABA + LAMA and LABA + LAMA + ICS groups. However, we did not find any significant differences of exacerbation, hospitalization and mortality during the follow-up among different inhalation therapies groups on less symptomatic COPD patients. Over half of the less symptomatic patients received inhalation therapy that were inconsistent with the GOLD document recommendations in a Chinese population in the real world. In fact, the single inhaled drug of LAMA should be recommended and pulmonary function is not a good indicator for the choice of initial inhalation therapy in less symptomatic COPD patients.KEY MESSAGESOver half of the less symptomatic COPD patients received inhalation therapy that were inconsistent with the GOLD document recommendations in a Chinese population in the real world.The clinicians should offer a single inhaled drug of LAMA to less symptomatic COPD patients and pulmonary function is not a good indicator for the choice of initial inhalation therapy. Over half of the less symptomatic COPD patients received inhalation therapy that were inconsistent with the GOLD document recommendations in a Chinese population in the real world. The clinicians should offer a single inhaled drug of LAMA to less symptomatic COPD patients and pulmonary function is not a good indicator for the choice of initial inhalation therapy.

In this paper, we have synthesized a new metal chelating agent HPM and characterized it by NMR, IR and LC-MS/MS measurements. The HPM obtained three pKa values: pKa1=3.1988, pKa2=4.4157 and pKa3=9.0091. The pKa1 is due to the carboxyl group on the C11 position, the pKa2 is due to the hydroxyl group on the C6 position and the pKa3 is due to the NH2+ on the N8 position.

The study aimed to establish a high-performance liquid chromatography (HPLC) method for the quantitative analysis of the related substances of bedaquiline fumarate. Nuclear magnetic resonance and mass spectrometry were used for characterization and assay. A chromatographic method was used for separation. The conditions used were: gradient elution system composed of methanol 0.01mol/L KH2PO4 and 0.01 mol/L K2HPO4 (pH = 4.1) with a flow rate of 1 mL/min, at 224 nm as the detection wavelength. In this study, three degradation products of bedaquiline fumarate have been disclosed for the first time. The related impurities and degradation products of the drug were well separated. The method provided linear responses within the concentration range, which varied from 0.20 to 10.08 μg/mL with limits of detection of 0.10 μg/mL and limits of quantification of 0.20 μg/mL. The mean percent recovery varied between 91.64 and 105.89%. The method was validated for other parameters such as specificity, stability, and robustness. This method was validated and worked well for the impurity studies and quality control analysis of the laboratory-prepared samples of bedaquiline fumarate.

Mesenchymal stem cell-derived extracellular vesicles (MSCs-EVs) possess cardioprotection in acute myocardial infarction. Nevertheless, the therapeutic intervention potential and the molecular mechanism of EVs from NMN (Nicotinamide mononucleotide) preconditioned hUCMSCs (N-EVs) in acute myocardial infarction remains unknown. In the present study, EVs from hUCMSCs (M-EVs) and N-EVs were identified by electron microscopy, immunoblotting and nanoparticle tracking analysis. Compared with M-EVs, N-EVs significantly increased the proliferation, migration, and angiogenesis of HUVECs. Meanwhile, N-EVs markedly reduced apoptosis and cardiac fibrosis and promoted angiogenesis in the peri-infarct region in the MI rats. A high-throughput miRNA sequencing and qPCR methods analysis revealed that miR-210-3p was abundant in N-EVs and the expression of miR-210-3p was obviously upregulated in HUVECs after N-EVs treated. Overexpression of miR-210-3p in HUVECs significantly enhanced the tube formation, migration and proliferative capacities of HUVECs. However, downregulation of miR-210-3p in HUVECs markedly decreased the tube formation, migration and proliferative capacities of HUVECs. Furthermore, bioinformatics analysis and luciferase assays revealed that EphrinA3 (EFNA3) was a direct target of miR-210-3p. Knockdown of miR-210-3p in N-EVs significantly impaired its ability to protect the heart after myocardial infarction. Altogether, these results indicated that N-EVs promoted the infarct healing through improvement of angiogenesis by miR-210-3p via targeting the EFNA3. Graphical Abstract Created with Biorender.com.

Polychlorinated biphenyls (PCBs) are nowadays mainly unintentionally produced, since their use and manufacture has been banned. PCBs, especially those dioxin-like compounds, have been proven harmful to ecosystems and human health. Detailed information about the spatiotemporally distribution of unintentionally produced PCBs (UP-PCBs) is crucial for understanding the environmental fate and associated health risks. However, research estimating UP-PCBs emission in China is limited. In this study, a source-specific and year-varying emission factor (EF) dataset was used to compile provincial emissions of 12 dioxin-like UP-PCBs congeners for 66 sources in the Chinese mainland from 1960 to 2019. In addition to that, emissions for 2019 were allocated into 0.1∘×0.1∘ grids. The results indicated that historical national UP-PCBs emission represented an increasing trend until around 1995 and then showed an overall decreasing trend from 1995 to 2019. Cement production was the largest UP-PCBs emission source in the Chinese mainland. Geographically, East and North China contributed the largest UP-PCBs emission across the Chinese mainland. High emission densities occurred mainly in the densely populated and well-developed regions such as Yangtze River Delta Urban Agglomeration and Pearl River Delta. Furthermore, highly positive correlations of emission densities with population and GDP densities were identified.

A novel method was established for analysing trace four acidic phytohormones, namely, indole-3-acetic acid, 3-indolebutyric acid, abscisic acid, and 1-naphthylacetic acid, using magnetic ordered mesoporous carbon (MOMC). MOMC was facilely synthesised via self-assembly strategy with a direct carbonisation process. The properties of MOMC were characterised using various instruments. MOMC exhibited excellent adsorption capacity towards the analytes. Various critical parameters which may influence the enrichment efficiency were evaluated, including amount of MOMC, extraction conditions, and desorption conditions. An efficient method based on MOMC magnetic solid-phase extraction coupled with ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) was developed to analyse the trace four acidic phytohormones, with good correlation coefficients (R2 = 0.9965–0.9998) and low limits of detection (0.13–9.7 ng L−1, S/N = 3). Trace acidic phytohormones in Agaricus bisporus and Hypsizygus marmoreus samples were determined with satisfactory recoveries (91.8–108%) and reproducibility (2.6–6.3%). The features indicated that MOMC provides an efficient platform for mushroom sampling; the developed method is convenient, promising, and sensitive for the detection of trace phytohormones in complicated mushroom samples.

WiFi-based imaging enables pervasive sensing in a privacy-preserving and cost-effective way. However, most of existing methods either require specialized hardware modification or suffer from poor imaging performance due to the fundamental limit of off-the-shelf commodity WiFi devices in spatial resolution. We observe that the recently developed reconfigurable intelligent surface (RIS) could be a promising solution to overcome these challenges. Thus, in this paper, we propose a RIS-aided WiFi imaging framework to achieve high-resolution imaging with the off-the-shelf WiFi devices. Specifically, we first design a beamforming method to achieve the first-stage imaging by separating the signals from different spatial locations with the aid of the RIS. Then, we propose an optimization-based super-resolution imaging algorithm by leveraging the low rank nature of the reconstructed object. During the optimization, we also explicitly take into account the effect of finite phase quantization in RIS to avoid the resolution degradation due to quantization errors. Simulation results demonstrate that our framework achieves median root mean square error (RMSE) of 0.03 and median structural similarity (SSIM) of 0.52. The visual results show that high-resolution imaging results are achieved with simulation signals at 5 GHz that are matched with commercial WiFi 802.11n/ac protocols.

N6-methyladenosine (m6A), the most abundant modification in mRNAs, has been defined as a crucial modulator in the progression of acute myelogenous leukemia (AML). Identification of the key regulators of m6A modifications in AML could provide further insights into AML biology and uncover more effective therapeutic strategies for patients with AML. Here, we report overexpression of YTHDF1, an m6A reader protein, in human AML samples at the protein level with enrichment in leukemia stem cells (LSC). Whereas YTHDF1 was dispensable for normal hematopoiesis in mice, depletion of YTHDF1 attenuated self-renewal, proliferation, and leukemic capacity of primary human and mouse AML cells in vitro and in vivo. Mechanistically, YTHDF1 promoted the translation of cyclin E2 in an m6A-dependent manner. Structure-based virtual screening of FDA-approved drugs identified tegaserod as a potential YTHDF1 inhibitor. Tegaserod blocked the direct binding of YTHDF1 with m6A-modified mRNAs and inhibited YTHDF1-regulated cyclin E2 translation. Moreover, tegaserod reduced the viability of patient-derived AML cells in vitro and prolonged survival in patient-derived xenograft models. Together, our study defines YTHDF1 as an integral regulator of AML progression by regulating the expression of m6A-modified mRNAs, which might serve as a potential therapeutic target for AML.Significance:The m6A reader YTHDF1 is required for progression of acute myelogenous leukemia and can be targeted with the FDA-approved drug tegaserod to suppress leukemia growth.

Aims To explore the predictive value of ferroptosis-related (FR) biomarkers for diabetic kidney disease (DKD) in patients with type 2 diabetes mellitus (T2DM). Methods This prospective observational study enrolled patients with T2DM at the Second Hospital of Jilin University between December 2021 and March 2022. DKD was measured by the urinary albumin-to-creatinine ratio. Receiver operating characteristic curve (ROC) analysis was performed to assess the predictive value of ferroptosis-related biomarkers for DKD.The risk factors for massive proteinuria were performed by multivariable logistic regression analysis. Results Finally, 118 patients (53.0 ± 12.2 years, 76 males) were enrolled, 52 of them without DKD (had normal proteinuria), while 66 with DKD. (Forty-one had microproteinuria, and 25 had massive proteinuria.) FR biomarkers, including acyl-CoA synthase long chain family member 4 (ACSL4), malondialdehyde (MDA), and reactive oxygen species (ROS), were significantly higher in the massive proteinuria group than in the other groups, while glutathione peroxidase 4 (GPX4) was significantly lower (all P < 0.05). The area under the ROC of the combination of GPX4, ACSL4, MDA, and ROS for predicting DKD was 0.804 (P < 0.001). Additionally, multivariate logistic regression analysis showed that the course of disease and ferritin levels were independent risk factors for massive proteinuria, while high serum iron, transferrin, and GPX4 levels were independent protective factors for massive proteinuria in patients with T2DM (all P < 0.05). Conclusions The GPX4, ACSL4, MDA, and ROS combination might have a good predictive value for DKD. Additionally, the course of disease, ferritin levels, serum iron, transferrin, and GPX4 were independently associated with massive proteinuria.