1. Synthesis and evaluation of tetrahydropyrazolopyridine inhibitors of anion exchange protein SLC26A4 (pendrin)
- Author
-
Mark J. Kurth, Peter M. Haggie, Marina E. Shatskikh, Julia Y. Lu, Jung-Ho Son, Alan S. Verkman, Amber A. Rivera, Joseph-Anthony Tan, Jie S. Zhu, and Puay-Wah Phuan
- Subjects
Pyridines ,Chronic rhinosinusitis ,Antiporter ,Clinical Biochemistry ,Pharmaceutical Science ,Pharmacology ,01 natural sciences ,Biochemistry ,Cystic fibrosis ,Mice ,Regioselectivity ,Drug Discovery ,Lung ,Inbred F344 ,Anion transporter ,Molecular Structure ,biology ,Ion exchange ,Chemistry ,Pharmacology and Pharmaceutical Sciences ,Transmembrane protein ,Sulfate Transporters ,5.1 Pharmaceuticals ,Respiratory ,Molecular Medicine ,Development of treatments and therapeutic interventions ,Medicinal & Biomolecular Chemistry ,Article ,Small Molecule Libraries ,Structure-Activity Relationship ,Medicinal and Biomolecular Chemistry ,Downregulation and upregulation ,SLC26A4 ,otorhinolaryngologic diseases ,medicine ,Animals ,Pendrin ,Hydrazine (antidepressant) ,Molecular Biology ,010405 organic chemistry ,Organic Chemistry ,medicine.disease ,Rats, Inbred F344 ,Rats ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Pyrazole ,biology.protein ,Pyrazoles - Abstract
Pendrin is a transmembrane chloride/anion antiporter that is strongly upregulated in the airways in rhinoviral infection, asthma, cystic fibrosis and chronic rhinosinusitis. Based on its role in the regulation of airway surface liquid depth, pendrin inhibitors have potential indications for treatment of inflammatory airways diseases. Here, a completely regioselective route to tetrahydro-pyrazolopyridine pendrin inhibitors based on 1,3-diketone and substituted hydrazine condensation was been developed. Structure-activity relationships at the tetra hydropyridyl nitrogen were investigated using a focused library, establishing the privileged nature of N-phenyl ureas and improving inhibitor potency by greater than 2-fold.
- Published
- 2019