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Protein detection is paramount across various scientific, clinical, and industrial domains. Accurate and sensitive detection of proteins is pivotal for understanding biological processes, diagnosing diseases, drug development, environmental monitoring, and ensuring food safety. Traditional protein detection methods encounter sensitivity, specificity, and ease of use challenges. Molecularly imprinted polymers (MIPs), with their tailored molecular recognition sites, offer a novel approach to address these limitations. When combined with electrochemical techniques, MIP-based electrochemical methods have emerged as a revolutionary technology, showcasing enhanced sensitivity and selectivity. This article provides a comprehensive overview of MIP-based electrochemical methods for protein detection, including the principles, engineering aspects, advantages, and potential applications. The aim is to elucidate the potential of this cutting-edge technology in reshaping protein detection and its promising role in advancing biosensing technologies.