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Dependence of biomass accumulation by melanin producer Pseudonadsoniella brunnea (Merepilaceae, Agaricomycotina) of the cultural medium content was aim of this study. The following 14 different types of cultural media were used: standard liquid nutrient media (Malt extract broth – МЕВ), Sabouraud Broth, potato-dextrose Broth, as well as the original (modified) nutritional media, based on Malt extract Broth, barley malt extract and Nutrient Broth. Various components were used for the modified media, in particular 0.9 % NaCl, monosaccharides (D-glucose, fructose, D (+) – xylose), disaccharides (sucrose, D (+) – maltose, D-lactose), and others. Cultivation of Ps. brunnea was carried out at pH 3-4, temperature + 24 ± 2 ºС for 28 days. The analysis of the results obtained shows that the following media: the liquid nutrient media of MEB, Sabouraud Broth, and barley malt extract with the addition of enzymatic peptone (1 %), yeast extract (0.5 %) and a mixture of these media in different proportions found to be the most optimal media for the accumulation of biomass of melanin producer Pseudonadsoniella brunnea. [ABSTRACT FROM AUTHOR]