Search

It has been established that activity of trehalosephosphate synthase (key enzyme of trehalosemycolates synthesis) under cultivation of Rhodococcus erythropolis EK-1 on the medium with n-hexadecane was 10 times higher, and amount of synthesized surface-active substances 4 times higher as compared to the growing of EK-1 strain on ethanol. Introduction of 0.2% of fumarate and 0.1% of citrate to the cultivation medium of R. erythropolis EK-1 with 2% of n-hexadecane, as well as 0.2% of fumarate to the medium with 2% of ethanol was accompanied by intensification of synthesis of surface-active trehalosemycolates, that is evidenced by the 1.3-1.6-fold increase of the amount of synthesized surface-active substances under such conditions, and by 3-5-fold increase of activity of phosphoenolpyruvate synthetase (key enzyme ofgluconeogenesis) and trehalosephosphate synthase. The paper is presented in Ukrainian.

The hexadecane oxidation of Rhodococcus erythropolls EK-1 was established to be catalyzed by the alkane hydroxylase complex containing Fe-S proteid rubredoxin. Alkane hydroxylase activity was increased twice in the presence of 10 mg/l iron ions in the cultivation medium. Sodium ions were shown to be an activator of alkane hydroxylase. Four types of alcohol dehydrogenases (NAD, NADP, pyrroloquinoline quinone and N,N-dimethyll-4-nitrosoaniline-dependent enzymes) were found in hexadecane-grown cells of R. erythropolis EK-1. The obtained data are the basis for the improvement of surface active substances technology.