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The issue of cattle reproduction was and still is one of the main tasks in cattle breeding. Losses of farms from infertility of cows are quite significant and range within 3.19-5.41 per 1 day of infertility. Mycotoxins produced by fungi of the Fusarium family, namely deoxynivalenol (DON) and zearalenone (ZEN) adversely affect not only the functioning of all organs and systems of the cow’s body, but also produce an immunosedative effect. The purpose of this study was to establish the effect of the DON and ZEN complex on the main indicators of the immune response of cows and its correction in a comparative aspect using a feed additive based on zeolite and organic acids and recombinant α-, g-interferons. The study material was the blood of cows (serum and stabilized) sick with mycotoxicosis caused by the association of DON and ZEN. Methods used: photonephelometric using E. coli test culture, spontaneous rosette formation with sheep red blood cells according to M. Jondal, modified method of rosette formation according to M. Wansbrough-Jones, R. Limatibul’s method, simple radial immunodiffusion in gel according to G. Mancini, precipitation in a polyethylene glycol solution according to M. Digeon. Experimental studies were performed on black-spotted cows in farms of the Sumy Oblast. The dynamics of the immune indicators of cows during the development of mycotoxicosis and upon treatment with products zeolite-based, organic acids, and an aqueous solution of recombinant α-, g-interferons were studied. It was found that the indicator of bactericidal, lysozyme, complementary, and phagocytic activity of cow blood serum under treatment increased to the indicator inherent in healthy animals. The dynamics of immunoglobulins in the treatment with zeolite and organic acids and recombinant α-, g-interferons was investigated, and an increase to the level of intact cows was established. It was proved that the indicators of the immune response of cows upon using a feed additive based on zeolite and organic acids at a dose of 2.5 kg per tonne of fodder and a preparation based on an aqueous solution of recombinant α-, g-interferons at a dose of 3 ml per animal were significantly higher

The results of the dependence of obstetric diseases of cows in the occurrence of subclinical abortions against the background of the development of metritis under the influence of mycotoxins are given. Subclinical metritis is a fairly common phenomenon in cows, which causes significant economic losses. At the same time, the number of subclinical metritis in cows correlates with the number of obstetric pathology. Due to the negative impact of mycotoxins, in particular zearalenone, the balance of sex hormones is disturbed, which creates conditions for the development of saprophytic microflora in the organs of the reproductive system of cows. At the same time, deoxynivalenol helps to reduce the resistance of the cow's body in general and mucous membranes, in particular, which contributes to the development of inflammatory processes in the uterus. These processes are often subclinical. The aim of the work was to find out the role of obstetric diseases in the development of subclinical metritis and to develop clinical and prognostic tests for the occurrence of metritis and the basis for the analysis of obstetric pathology. The spread of obstetric pathology was established depending on the way cows are kept. Thus, in farms where cows were kept on tethers, the number of obstetric pathologies was 15.5 times higher. However, the number of inflammatory processes in the organs of the reproductive system in such farms was lower by 12 %. A decrease in the level of T- and Blymphocytes in the blood and suppression of their functional activity was established, which is manifested by a decrease in the level of immunoglobulin G and has a significant effect on the increase in the frequency of subclinical abortions. The dependence of the reproductive capacity of cows on the frequency of subclinical and chronic inflammatory processes of the reproductive organs, which occur against the background of mycotoxicosis, was established. The influence of mycotoxins on the functioning of the ovaries and uterus was studied, and an increase in the number of such cases was established in farms with tethered cows. The correlation between the contamination of cow feed and the reduction of fertilization in cows was found. An increase in the number of subclinical abortions under the influence of zearalenone, which is found in large quantities in feed, has been established. The dependence of ovarian disorders on increasing productivity in cows was investigated. [ABSTRACT FROM AUTHOR]

Toxicobiological eff ect of mycotoxins association of the Penicillium and Fusarium fungus (T-2 toxin at a concentration of 0.1 mg/kg, fumonisin B1 ‒ 0.5 mg/kg, vomitoxin (DON) ‒ 0.1 mg/kg, penicillic acid ‒ 1 mg/kg) was accompanied by the development of a complex pathological process in weaned piglets. In this regard, the detoxifi cation and sorption capacity of the complex feed additive "Harufi x+" based on mannanoligosaccharides was studied. The additive eff ect on the resorptive activity of mineral and vitamin nutrients of feed under the normal feeding conditions and in case of contamination with mycotoxins has been studied. The use of enterosorbent off set the toxic eff ects of micromycete metabolites, which contributed to the growth of piglets. Thus, weight growth rate increase of the piglets in group 1 (i.e., those whose diet included the additive, unlike the diet of the animals in control group) constituted 16 %, while their average weight growth rate was high and constituted 1.96 kg per day. In addition, during the study of calcium, phosphorus, magnesium, ferum, zinc, copper and manganese in the piglets blood was not found excretion of these elements with a sorbent, moreover, noted the normalization their blood level. The study content of vitamins A and E, the same as with mineral nutrients, has not been established decrease during treatment with study the pharmaceutical. The obtained results testify the active absorption in the gastrointestinal tract of the vitamin components within the fodder combined with fodder additive “Harufi x+” and high biological accessibility of its transport forms. The effi ciency of the additive can be explained by its composition, namely the complex of mineral and organic components that are formed by modifi cation of the organic cations of the mineral surface.

In the article presents results of the research of pre-slaughter and post-slaughter characteristics of broiler chickens carcasses at the mixed T-2 and zearalenone toxicosis and use of sorbents. Indexes of body mass and semie-viscerated carcass mass in experimental groups broiler chickens, which were used by «Presorb» and coal-bentonite sorbent, was closest related to the indexes of control group broiler chickens. [ABSTRACT FROM AUTHOR]

The research results confirm that at the joint action of ochratoxin A and deoxynivalenol in blood plasma broiler chickens observed to increase in activity of gamma-glutamyltranspeptidase (GGT) which indicates about affection of hepatobiliary system. Application of enterosorbents to broiler chickens in research groups reduces toxic effects of mycotoxins to state of plasma membranes and promotes stabilization of gammaglutamyltranspeptidase activity. [ABSTRACT FROM AUTHOR]

This article describe research results of broiler chicken meat organoleptic indicators during mixed T-2 and zearalenotoxicosis while using sorbents. During experiment we fed broiler chicken with enterosorbents: Carbovet, Xerogel, Toxynil and Revital (at the first series of experiment); BioTox, Xerogel with Inulin, Presorb and сoal-bentonite sorbent (second series of experiments). At the time of organoleptic indicators research any differences compared to the control group was not found. Using the sorbents due to the mixed T-2 and zearalene toxicosis positively affects organoleptic indicators of broilers chicken meat. For these mycotoxicosis it is improbable to get less indicators than for control group in appearance taste, aroma, tenderness and succulence of broilers chicken meat. The established differences in the organoleptic indicators of broiler chicken meat may be due to the negative effect of T-2 toxin and zearalenone. [ABSTRACT FROM AUTHOR]

The research results confirm that there is a significant increase of the alkaline phosphatase activity in the blood plasma of broiler chickens at the combained action of ochratoxin A and deoxynivalenol. As is known, increasing of alkaline phosphatase activityin the blood plasma is associated with its production by the cells of the bile ducts, cholestasis and disbalance of the excretion of the enzyme in bile. In the blood plasma of broiler chickens of experimental groups which used enterosorbents for mixed mycotoxicosis the alkaline phosphatase activity in all the research periods did not differ from the index of control group, but was significantly less than the index of experimental group which fed only feed with mycotoxins. Using of the enterosorbent for broiler chickens of the experimental groups (Toxy-Nil® Plus Unike - the second experimental group, Mycofix® Plus 3.E - the third experimental group, activated birch carbon - the fourth experimental group) reduces the toxic effect of ochratoxin A and deoxynivalenol, that is confirmed by the normalization of activity the enzyme under study. [ABSTRACT FROM AUTHOR]

In the article presents results of the research of preslaughter and postmortem characteristics of broiler chickens carcass at the mixed T-2 and zearalenone toxicosis and use of sorption preparations. Applying of sorbents to broiler chickens at the mixed T-2 and zearalenone toxicosis provides an increase in body weight, carcass weight and yield. [ABSTRACT FROM AUTHOR]

The research results showed that the combined ochra- and deoxynivalenol toxicosis broiler chickens to manifest violation of transaminase activity. Application of enterosorbents to broiler chickens in research groups reduces toxic effects of ochratoxin A and deoxynivalenol and promotes normalization of transaminase activity. [ABSTRACT FROM AUTHOR]

This study describes the principles of development of investigations model of mixed T-2 and ZEA mycotoxicosis in chickens-broilers. The investigations were carried out in three stages. The first stage was the examination of the individual ingredients of diet to the content of mycotoxins. The second stage of our investigation was to study the combined effect of Т-2 toxin A and zearalenone on broiler chickens organism. The third stage - the veterinary-sanitary assessment of products of slaughter and determination of residual amounts of mycotoxins. [ABSTRACT FROM AUTHOR]