1. [Cardiomyocyte differentiation of individual clones murine induced pluripotent stem cells].
- Author
-
Malysheva SV, Budash HV, Bil'ko NM, and Heschheller J
- Subjects
- Animals, Ascorbic Acid pharmacology, Cell Differentiation, Cells, Cultured, Clone Cells, Embryo, Mammalian, Embryoid Bodies drug effects, Embryoid Bodies physiology, Fibroblasts cytology, Fibroblasts drug effects, Fibroblasts physiology, Genes, Reporter, Green Fluorescent Proteins, Induced Pluripotent Stem Cells drug effects, Induced Pluripotent Stem Cells physiology, Mice, Mice, Transgenic, Myocytes, Cardiac drug effects, Myocytes, Cardiac physiology, Plasmids, Transfection, DNA Transposable Elements, Embryoid Bodies cytology, Induced Pluripotent Stem Cells cytology, Myocytes, Cardiac cytology
- Abstract
Cardiomyocyte differentiation of certain clones of murine induced pluripotent stem cells (iPS) was estimated. iPS were obtained due to reprogramming of murine embryonic fibroblasts with transposon-based Sleeping beauty plasmids as sene delivery systems. Differentiation was performed in suspension culture and in attached to tissue-culture plates embryoid bodies (EBs). Ascorbic acid was applied as inductor. According to the obtained results, the differentiation was tenfold more effective in attached EBs. Ascorbic acid stimulated the generation of cardiomiocytes.
- Published
- 2013