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Your search keyword '"Genes, Reporter"' showing total 4 results
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4 results on '"Genes, Reporter"'

1. [Differentiation of mesenchymal stem cells of adipose tissue].

Author

Salyutin RV, Zapohlska KM, Palyanytsya SS, Sirman VM, and Sokolov MF

Subjects
Adipocytes metabolism, Adipose Tissue metabolism, Aggrecans genetics, Aggrecans metabolism, Alkaline Phosphatase genetics, Alkaline Phosphatase metabolism, Animals, Biomarkers metabolism, Cell Differentiation, Chondrocytes cytology, Chondrocytes metabolism, Gene Expression, Genes, Reporter, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Immunohistochemistry, Mesenchymal Stem Cells metabolism, Mice, Mice, Transgenic, Multipotent Stem Cells metabolism, Osteoblasts cytology, Osteoblasts metabolism, Primary Cell Culture, Adipocytes cytology, Adipose Tissue cytology, Mesenchymal Stem Cells cytology, Multipotent Stem Cells cytology
Abstract

Experimental investigation were conducted with the objective to determine a stem cells, capacity to differentiate in adipogenic direction, if they were obtained from adipose tissue. The investigation results have witnessed, that the cells, obtained from adipose tissue, are capable for a tissue-speciphic differentiation in osteogenic, chondrogenic, and, principally--in adipogenic direction, what confirms a multypotent nature of mesenchymal stem cells of adipose tissue. Adipose tissue constitutes an alternative to the bone marrow, as a source of multipotent mesenchymal stem cells, which may be applied in further investigations, concerning determination of their defense possibility for the transplanted autologous adipose tissue from the tissue resorption, made in a lipophiling way.

Published
2015

2. [Cardiomyocyte differentiation of individual clones murine induced pluripotent stem cells].

Author

Malysheva SV, Budash HV, Bil'ko NM, and Heschheller J

Subjects
Animals, Ascorbic Acid pharmacology, Cell Differentiation, Cells, Cultured, Clone Cells, Embryo, Mammalian, Embryoid Bodies drug effects, Embryoid Bodies physiology, Fibroblasts cytology, Fibroblasts drug effects, Fibroblasts physiology, Genes, Reporter, Green Fluorescent Proteins, Induced Pluripotent Stem Cells drug effects, Induced Pluripotent Stem Cells physiology, Mice, Mice, Transgenic, Myocytes, Cardiac drug effects, Myocytes, Cardiac physiology, Plasmids, Transfection, DNA Transposable Elements, Embryoid Bodies cytology, Induced Pluripotent Stem Cells cytology, Myocytes, Cardiac cytology
Abstract

Cardiomyocyte differentiation of certain clones of murine induced pluripotent stem cells (iPS) was estimated. iPS were obtained due to reprogramming of murine embryonic fibroblasts with transposon-based Sleeping beauty plasmids as sene delivery systems. Differentiation was performed in suspension culture and in attached to tissue-culture plates embryoid bodies (EBs). Ascorbic acid was applied as inductor. According to the obtained results, the differentiation was tenfold more effective in attached EBs. Ascorbic acid stimulated the generation of cardiomiocytes.

Published
2013

3. [Characterization of Streptomyces globisporus 1912 lnd-cluster region containing lndY, lndYR, lndW2 and lndW genes].

Author

Tsypik O, Ostash B, Rebets' Iu, and Fedorenko V

Subjects
Aminoglycosides metabolism, Catechols metabolism, Dioxygenases metabolism, Genes, Reporter, Ligands, Open Reading Frames, Peptide Hydrolases genetics, Peptide Hydrolases metabolism, Promoter Regions, Genetic, Streptomyces metabolism, Dioxygenases genetics, Gene Expression Regulation, Bacterial, Genes, Bacterial, Multigene Family, Mutant Chimeric Proteins genetics, Streptomyces genetics
Abstract

Streptomyces globisporus 1912 lnd-cluster region which flanks structural lndZ5-lndZ6 genes contains four open reading frames lndW, lndW2, lndYR and lndY. The latter one encodes putative proteinase which can regulate landomycin production and morphogenesis like LndYR. However, results of lndY overexpression and gene knockout showed that lndY did not participate in regulation of landomycin production and morphogenesis of Streptomyces globisporus 1912. Using transcriptional fusion of promoter lndWp to catechol dioxygenase reporter gene xylE the temporal character of interaction of promoter lndWp and repressor LndYR was studied.

Published
2013

4. [Construction of a new bifunctional vector which contains EGFP-gene].

Author

Luk'ianchuk VV and Polishchuk LV

Subjects
Ampicillin chemistry, Culture Media, DNA Restriction Enzymes metabolism, DNA, Bacterial genetics, Fluorescence, Genes, Reporter, Genetic Vectors genetics, Kanamycin chemistry, Thiostrepton chemistry, Transformation, Bacterial, Cloning, Molecular methods, DNA, Bacterial chemistry, Escherichia coli genetics, Genetic Vectors chemistry, Green Fluorescent Proteins analysis, Streptomyces genetics
Abstract

A number of bifunctional vector structures (pTpE, pTpE5, pTpH2, pTpH7, pTpS1) with molecular dimension of 11.3 kb has been created. Vector plasmids can be replicated, sustainably maintained and expressed both in Gram-negative (Escherichia coli XL1 Blue) and in Gram-positive (Streptomyces levoris 165) cultures. The given bifunctional vectors contain genes of resistance to 3 antibiotics. Genes of resistance to ampicillin and canamycin are expressed in E. coli cells, gene of resistance to thiostrepton ensures the resistance of streptomycete cells to the given antibiotic. It is proved that all the studied plasmids contain active egfp-gene which allows the visual study in vivo of the functioning of the poducts ofpEGFP-C1 gene cloned to polylinker.

Published
2012

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