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Reconstruction of P. vitale glucose oxidase from apeonzyme and coenzyme (FAD) was studied as affected by iodine acetate as well as mercury and calcium ions. Mercury ions, iodine acetate as well as the labilizing fraction (flavin adenine-containing component) are established to inhibit the reconstruction affecting the sulphydryl groups of the apoenzyme which take part in addition of FAD to it. Calcium ions prevent the effect of the "labilizing" fraction, iodine acetate and mercury ions on the glucose oxidase apoenzyme as well as the effect of mercury ions on the glucose oxidase coenzyme. The effect of Ca2+ consists in "labilizing" fraction-calcium complexing.

The article deals with conditions for splitting the Penicillium vitale glucosooxidase molecule into apoenzyme and coenzyme, for reconstruction of the enzyme from apoenzyme and FAD as well as for the effect of some factors ("labilizing" fraction, dithiotreitol) on it. Specific activity of the reconstructed enzyme is on the average 85% of the initial enzyme specific activity: "the labilizing" fraction inhibits reconstruction of glucosooxidase from apoenzyme and FAD, that is due to oxidation of the apoenzyme sulphydryl groups. Possible participation of sulphydryl groups in adding FAD to apoenzyme is under discussion.