1. [Functional state of the ileum, cationic peptids and zinc content in the Paneth cells of the rats following lincomycin, indomethacin and methotrexate andministration].
- Author
-
Horokhovs'kyĭ IeIu, Ieshchenko IuV, Bovt VD, Ieshchenko VA, and Iurchuk IIe
- Subjects
- Animals, Antibiosis drug effects, Antimicrobial Cationic Peptides antagonists & inhibitors, Antimicrobial Cationic Peptides biosynthesis, Bacterial Translocation drug effects, Gram-Negative Bacteria drug effects, Gram-Negative Bacteria physiology, Gram-Positive Bacteria drug effects, Gram-Positive Bacteria physiology, Ileum microbiology, Ileum physiology, Intestinal Mucosa microbiology, Intestinal Mucosa physiology, Male, Paneth Cells microbiology, Paneth Cells physiology, Permeability drug effects, Rats, Symbiosis drug effects, Zinc metabolism, Ileum drug effects, Indomethacin pharmacology, Intestinal Mucosa drug effects, Lincomycin pharmacology, Methotrexate pharmacology, Paneth Cells drug effects
- Abstract
In the paper the relation between the changes of a functional condition of ileum, composition of its microflora and secretory activity of Paneth cells has been investigated. Administration of lincomycin led to a reduction of total of symbiotic bacteria and appearance of opportunistic microflora. Statistically insignificant changes of speed of transit of intestinal contents and damage of barrier function of an intestinal epithelium were noted. The intestinal epithelium was characterized by signs of inflammation. Administration of indomethacin and methotrexate led to significant changes of a functional state of a small intestine of rats. The speed of transit of intestinal contents following indomethacin administration increased by 30%, and, on the contrary, decreased by 90% following administration of methotrexate. The ability of epithelium of ileum to adsorb vital dye in both cases increased (by 32 and 51%, respectively) indicating the damage of barrier function. Thus there were significant changes in structure of microflora of ileum: reduction of quantity symbiotic and emergence of opportunistic microflora, a translocation of bacteria to a mucous wall. It was concluded that animal of all three experimental groups had a reduction of the contents of cationic proteins and zinc in the secretory granules of Paneth cells. These results can testify that balance changes between simbiotic and opportunistic microflora are a factor which has an impact on intestine damage and also causes activation of secretory function of Paneth cells.
- Published
- 2013