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Fatty acid composition of phospholipids which are the main structural elements of the cytoplasmic and cell membranes studied. It was established that in the fatty acid composition of blood plasma, liver, skeletal muscles phospholipids in rats with experimental hyperсholesterinemia an increase in saturated fatty acids with even and odd number of carbon atoms in chain and monounsaturated fatty acids of family n-9 took place. However, the relative content of polyunsaturated fatty acids of families n-3 and n-6 decreased. Inverse patterns due to the above fatty acids in fatty acid composition of blood plasma, liver, skeletal muscles phospholipid in rats with experimental hypercholesterinemia corrected by fish oil. It decreased the relative content of saturated fatty acids with even and odd number of carbon atoms in chain and monounsaturated fatty acids of family n-9, and increased the relative content of polyunsaturated fatty acids of families n-3 and n-6. Feeding with fish oil corrected fatty acid composition of blood plasma, liver and skeletal muscle phospholipids in rats with experimental hypercholesterolemia.