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In this study, bulblets samples were chosen in produced by in vitro micropropagation method, analyzed by observing the sample from 3 different time periods interferences (i.e. at the end of the first, second and third month). After extraction of these bulblets samples, alkaloid (nitrogen compounds) profile was determined by GC-MS; and was compared with the bulblets grown by in vivo method. Micropropagation analysis, allowed us to find the most suitable sampling period which was close to in vivo condition. In this research, twin scales of L. candidum were used as explant and as a result of regeneration, GC-MS was analyzed in 3 different nutrient media at 3 different sampling periods. The result of GC-MS analysis showed that the total obtained alkaloid percentage is the closed to the control (13.11%) in the third sample (7.42%) in the second nutrient media (0.2 mg/L NAA + 0.4 mg/L TDZ). The highest percentage of alkaloid (1H-Pyrrole, 2,5-dihydro-1-nitroso-) was obtained from the second nutrient medium and the third sample. [ABSTRACT FROM AUTHOR]