Search

Objective: This study aimed to determine the prevalence and phenotypes of macrolide, lincosamide, and streptogramın B (MLSB) resistance in Staphylococcus aureus strains isolated from clinical samples and to assess the efficacy of mupirocin and fusidic acid against these isolates. Methods: Between January 2021 and December 2022, S. aureus strains isolated from clinical samples were identified by VITEK® MS (bioMérieux, France), and their antibiotic susceptibilities were determined using VITEK® 2 (bioMérieux, France). The disk approximation method was used to determine the MLSB resistance phenotypes using erythromycin (15 μg) and clindamycin (2 μg). For fusidic acid, a MIC of ≤1 mg/L was considered susceptible, and a MIC of >1 mg/L was considered resistant. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) 2020 guidelines were followed, with a MIC of ≤1 mg/L considered sensitive, a MIC of 2-256 mg/L indicating low-level resistance, and a MIC of >256 mg/L indicating high-level resistance for mupirocin. Results: The MLSB resistance phenotypes of the strains were as follows: 8.9% inducible (iMLSB), 4.6% chromosomal (cMLSB), 12.8% MS type, 0.8% L type, and 72.92% erythromycin and clindamycin sensitive were detected. Mupirocin resistance was detected 7.2% in 250 S. aureus strains, 14.3% in MRSA, and 2.1% in MSSA. Fusidic acid resistance was seen at 10.7% in 711 S. aureus strains, 20.1% in MRSA, and 5.5% in MSSA. Conclusion: When erythromycin resistance is detected in MRSA isolates, it is essential to determine macrolide resistance phenotypes with a simple and easy method such as the D-test and investigate clindamycin resistance for choosing appropriate antibiotics. Mupirocin resistance in S. aureus strains was higher in MRSA isolates than in MSSA isolates. It is noteworthy that fusidic acid resistance is frequently seen in both MRSA and MSSA isolates that are susceptible to erythromycin and clindamycin. In infections where fusidic acid is commonly used, it is necessary to monitor antibiotic susceptibilities closely. [ABSTRACT FROM AUTHOR]

Objective: Although Coagulase Negative Staphylococci (CoNS) species are among the microbiota members of the skin and mucosal surfaces, they cause infections especially related to medical devices such as catheters. Nowadays, antibiotic resistance is an important public health problem and rational use of antibiotics is of great importance in the treatment of bloodstream infections caused by CoNS species. The aim of this study was to investigate the antibiotic resistance profile and some virulence genes of CoNS isolates that cause catheter related bacteremia. Methods: In our study, 43 CoNS strains isolated from patients hospitalized in Manisa Celal Bayar University Hospital between 2016-2017, were evaluated for their susceptibility to ampicillin, clindamycin, mupirocin, linezolid, tigecycline, tetracycline, cefotaxime, gentamicin, vancomycin, ciprofloxacin, erythromycin and fusidic acid. The presence of clonal relationships and some virulence factors were also investigated. Susceptibility of cefoxitin (methicillin) was determined by disk diffusion and susceptibilities of other antibiotics were determined by microdilution method. AacA-aphD and aphA3 for aminoglycoside resistance, mecA and mecC for methicillin resistance, tetK and tetM for tetracycline resistance, ermA, ermB, ermC, msrA for macrolidelincosamide- streptogramin (MLS) type B resistance, icaA, IS256, nucA and sasX virulence genes were investigated by polymerase chain reaction (PCR). Clonal relationships at species level were examined by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR. Results: Considering all species of CoNS examined, the highest and the lowest resistance rates were observed for ampicilin (93 %) and linezolid (2 %), respectively. There wasn't any resistant CoNS isolate to tigecycline and vancomycin. We found that all the examined CoNS species were generally clustered in different clones. We detected mecA, aacA-aphD, aphA3, ermB, ermC and msrA antibiotic resistance genes as well as icaA, IS256 and sasX virulence genes in the studied strains. Conclusion: The results of our study revealed that the examined CoNS isolates causing bacteremia had acquired resistance to commonly used antibiotics and that these strains harboring various virulence genes. Therefore, those isolates and their infections should be carefully monitored. [ABSTRACT FROM AUTHOR]

Copyright of ANKEM Antibiyotik & Kemoterapi Dergisi is the property of ANKEM Antibiyotik & Kemoterapi Dergisi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of SDU Journal of Engineering Sciences & Design / Mühendislik Bilimleri ve Tasarım Dergisi is the property of Journal of Engineering Sciences & Design and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this study, it was aimed to investigate the prevalence of Staphylococcus aureus in poultry meats (32 chickens, 9 turkeys, 9 quails) sold in Isparta and Antalya provinces, and to determine the presence of enterotoxin genes as well as antibiotic resistance profiles and resistance genes. Baird Parker agar medium was used to isolate presumptive S. aureus from poultry meats. The species-level identification of isolates was done by polymerase chain reaction (PCR) using primer pair specific to the thermostable nuclease gene (nuc) in S. aureus. As a result of the PCR analysis, amplicons of 458 bp specific to the nuc gene were obtained in 16 of 130 presumptive S. aureus isolates. The frequency of S. aureus in poultry meat samples was found to be 20% (10/50). Coagulase test showed that all the S. aureus isolates were coagulase positive. Disk diffusion test showed that all of the isolates (100 %) were susceptible to chloramphenicol and teicoplanin and resistant to penicillin G. It was determined that 81.25 % (13/16) of the isolates were methicillin resistant S. aureus (MRSA). The most common antibiotic resistance gene in S. aureus strains was found to be blaZ (62.5 %, 10/16) as a result of PCR. The presence of sea, seb, sec, sed and see genes was not detected in any of the S. aureus strains. [ABSTRACT FROM AUTHOR]

Aim: In this study, we aimed to investigate the prevalence of nasal carriage of Staphylococcus aureus in our hospital staff, and antibiotic susceptibility of these strains to methicillin and some other antibiotics. Methods: In this study, involving personnel working in operating room, neonatal and intensive care units of our hospital, nose cavity swab samples were taken from 81 subjects using cotton swabs soaked into serum physiologic. Single colony inoculation was performed on 5% sheep blood agar. The samples were incubated at 37oC for 24 hours. The Catalase and coagulase tests were performed on the strains which showed gram-positive cocci. We evaluated antimicrobial susceptibility of all S. aureus strains to oxacillin and several other antibiotics by disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) recommendations.. Results: Nasal S. aureus carrier was detected in 21 subjects (25.9%). Among all S. aureus isolated from nasal area, nine (11.1%) were methicillin-resistance and 12 (14.8%) were methicillin-susceptible S. aureus. When the occupation was considered, the carriage rates were 20.8% among doctors, 25% among auxiliary health-care workers and 29.7% among health-care workers other than doctors. There was no significant difference in carriage rate between these groups (p=0.239). The isolated strains showed no resistance to mupirocin and teicoplanin. Intranasal administration of mupirocin to all carriers for five days resulted in elimination of staphylococci with a success rate of 100%. Conclusion: According to these results, we assume that the nasal carriage of methicillin-resistant S. aureus is not an important issue in our hospital. However, it was concluded that topical mupirocin is an effective agent to be used securely in the elimination of S. aureus colonization in nasal carriers. (The Me di - cal Bul le tin of Ha se ki 2012; 50: 48-52)

Aim: In this study, we aimed to investigate the prevalence of nasal carriage of Staphylococcus aureus in our hospital staff, and antibiotic susceptibility of these strains to methicillin and some other antibiotics. Methods: In this study, involving personnel working in operating room, neonatal and intensive care units of our hospital, nose cavity swab samples were taken from 81 subjects using cotton swabs soaked into serum physiologic. Single colony inoculation was performed on 5% sheep blood agar. The samples were incubated at 37oC for 24 hours. The Catalase and coagulase tests were performed on the strains which showed gram-positive cocci. We evaluated antimicrobial susceptibility of all S. aureus strains to oxacillin and several other antibiotics by disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) recommendations.. Results: Nasal S. aureus carrier was detected in 21 subjects (25.9%). Among all S. aureus isolated from nasal area, nine (11.1%) were methicillin-resistance and 12 (14.8%) were methicillin-susceptible S. aureus. When the occupation was considered, the carriage rates were 20.8% among doctors, 25% among auxiliary health-care workers and 29.7% among health-care workers other than doctors. There was no significant difference in carriage rate between these groups (p=0.239). The isolated strains showed no resistance to mupirocin and teicoplanin. Intranasal administration of mupirocin to all carriers for five days resulted in elimination of staphylococci with a success rate of 100%. Conclusion: According to these results, we assume that the nasal carriage of methicillin-resistant S. aureus is not an important issue in our hospital. However, it was concluded that topical mupirocin is an effective agent to be used securely in the elimination of S. aureus colonization in nasal carriers. (The Medical Bulletin of Haseki 2012; 50: 48-52) [ABSTRACT FROM AUTHOR]