1. Association analysis of aquaporin 7 (AQP7) gene variants with semen quality and fertility in bulls
- Author
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MA, Teng-He, LIU, Ji-Feng, ZHAO, Rui-Feng, JIANG, Hao, DAI, Li-Sheng, ZHAO, Yu-Min, ZHAO, Zhi-Hui, and ZHANG, Jia-Bao
- Subjects
urogenital system ,Key words: AQP7 gene,single nucleotide polymorphisms,SNPs,association analysis,Simmental bulls,Charolais bulls - Abstract
Aquaporin 7 (AQP7) gene as a candidate Antifreeze gene was investigated and associated with fresh and frozen semen quality traits in 45 Simmental and Charolais bulls. PCR-SSCP, PCR-RFLP, and sequencing method were employed to detect the single nucleotide polymorphisms (SNPs) of the AQP7 gene. Two mutations, A264G and G371C, were identified. The allele frequencies were A (0.97, 0.96) to B (0.03, 0.04) and C (0.97, 0.91) to D (0.03, 0.09) in Simmental and Charolais bulls, respectively. A264G substitution was significantly associated with acrosome integrity ratio (P < 0.01) and motility (P < 0.05) in frozen semen. Meanwhile, G371C substitution was significantly associated with acrosome integrity ratio (P < 0.05), percentages of viable sperm (P < 0.05), and motility (P < 0.01) in frozen semen. The results prove that AQP7 gene SNPs are useful for marker-assisted selection in early seed selection in the bull industry.
- Published
- 2014